{"entity": "researcher", "timestamp": "2026-06-15T17:47:54.144Z", "family": "Ragaller", "given": "Franziska", "initials": "F", "orcid": "0000-0002-4148-262X", "affiliations": ["Science for Life Laboratory, Department of Women's and Children's Health, Karolinska Institutet, 17165 Solna, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/9ead87aca17f476b9febf3f020567c4b.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/9ead87aca17f476b9febf3f020567c4b"}}, "publications": [{"entity": "publication", "iuid": "197e14d7b03a454986e241aada0526a6", "links": {"self": {"href": "https://publications.scilifelab.se/publication/197e14d7b03a454986e241aada0526a6.json"}, "display": {"href": "https://publications.scilifelab.se/publication/197e14d7b03a454986e241aada0526a6"}}, "title": "Studying Macromolecular Composition in Cell-Cell Interfaces Using 3D Membrane Reconstitution Systems.", "authors": [{"family": "Ragaller", "given": "Franziska", "initials": "F", "orcid": "0000-0002-4148-262X", "researcher": {"href": "https://publications.scilifelab.se/researcher/9ead87aca17f476b9febf3f020567c4b.json"}}, {"family": "Schneider", "given": "Amelie Maribel", "initials": "AM"}, {"family": "Sjule", "given": "Ellen", "initials": "E"}, {"family": "Sun", "given": "Renhua", "initials": "R"}, {"family": "Han", "given": "Xiao", "initials": "X"}, {"family": "Andronico", "given": "Luca", "initials": "L", "orcid": "0000-0002-4326-073X", "researcher": {"href": "https://publications.scilifelab.se/researcher/8818b25185e54c7e9f120d34c1fc69cd.json"}}, {"family": "Jenkins", "given": "Edward", "initials": "E"}, {"family": "Dustin", "given": "Michael", "initials": "M"}, {"family": "Achour", "given": "Adnane", "initials": "A"}, {"family": "Sezgin", "given": "Erdinc", "initials": "E", "orcid": "0000-0002-4915-388X", "researcher": {"href": "https://publications.scilifelab.se/researcher/34d3b05d68d64f698ff08dc655d2fe26.json"}}], "type": "journal article", "published": "2026-03-24", "journal": {"title": "Adv Sci (Weinh)", "issn": "2198-3844", "pages": "e22443", "issn-l": null}, "abstract": "During direct communication between two cells, the plasma membranes of each cell serve as a platform for ligand-receptor interaction initiating downstream signaling cascades. In immune cell signaling, this cell-cell interface - the immune synapse - is highly spatiotemporally organized. Multiple stimulatory and co-stimulatory signals need to be integrated over time to ensure proper immune cell function. This process is still not fully understood given the vast complexity of interactions between proteins, lipids, glycocalyx and associated cortical actin cytoskeleton. Here, we presented a fully artificial model system to study the interface between two vesicles and a semi-artificial one between a live cell and a vesicle to reconstitute 3D contacts. We investigated the distribution and reorganization of immune cell proteins at artificial and semi-artificial contacts. We show the enrichment and depletion of different proteins in the synapse and how different peptides with varying affinity presented by the same MHC class I affect T cell activation. We further explored the distribution of glycocalyx elements and showed differential partitioning of different sugar moieties in the interface. While we focused on the T cell interface here, our model systems are powerful tools to study the distribution and reorganization of lipids, proteins and glycocalyx components at any cell-cell contact.", "doi": "10.1002/advs.202522443", "pmid": "41874480", "labels": {"Integrated Microscopy Technologies Stockholm": "Service"}, "xrefs": [], "notes": [], "created": "2026-03-26T19:13:29.629Z", "modified": "2026-03-26T19:13:29.781Z"}, {"entity": "publication", "iuid": "9e18ef322a3d4b42a45a7bbe96686990", "links": {"self": {"href": "https://publications.scilifelab.se/publication/9e18ef322a3d4b42a45a7bbe96686990.json"}, "display": {"href": "https://publications.scilifelab.se/publication/9e18ef322a3d4b42a45a7bbe96686990"}}, "title": "Studying macromolecular composition in cell-cell interfaces using 3D membrane reconstitution systems", "authors": [{"family": "Ragaller", "given": "Franziska", "initials": "F", "orcid": "0000-0002-4148-262X", "researcher": {"href": "https://publications.scilifelab.se/researcher/9ead87aca17f476b9febf3f020567c4b.json"}}, {"family": "Schneider", "given": "Amelie Maribel", "initials": "AM"}, {"family": "Sjule", "given": "Ellen", "initials": "E", "orcid": "0000-0003-0166-2826", "researcher": {"href": "https://publications.scilifelab.se/researcher/4e0fd8926ef843a0a8db936df7209107.json"}}, {"family": "Sun", "given": "Renhua", "initials": "R", "orcid": "0000-0002-8203-4946", "researcher": {"href": "https://publications.scilifelab.se/researcher/f639c1a9dca647c0b583ce74ab398f02.json"}}, {"family": "Han", "given": "Xiao", "initials": "X"}, {"family": "Jenkins", "given": "Edward", "initials": "E"}, {"family": "Dustin", "given": "Michael", "initials": "M"}, {"family": "Achour", "given": "Adnane", "initials": "A"}, {"family": "Sezgin", "given": "Erdinc", "initials": "E", "orcid": "0000-0002-4915-388X", "researcher": {"href": "https://publications.scilifelab.se/researcher/34d3b05d68d64f698ff08dc655d2fe26.json"}}], "type": "posted-content", "published": "2025-11-02", "journal": {"title": "biorxiv", "issn": null, "issn-l": null, "volume": null, "issue": null, "pages": null}, "abstract": null, "doi": "10.1101/2025.11.01.686034", "pmid": null, "labels": {"Integrated Microscopy Technologies Stockholm": "Service"}, "xrefs": [], "notes": [], "created": "2025-11-05T10:03:39.995Z", "modified": "2025-12-18T19:00:09.690Z"}, {"entity": "publication", "iuid": "48bf48701cf44883bb1fe87945bf952b", "links": {"self": {"href": "https://publications.scilifelab.se/publication/48bf48701cf44883bb1fe87945bf952b.json"}, "display": {"href": "https://publications.scilifelab.se/publication/48bf48701cf44883bb1fe87945bf952b"}}, "title": "Prodan-based solvatochromic probes for polarity imaging of organelles", "authors": [{"family": "Aknine", "given": "Nathan", "initials": "N"}, {"family": "Holban", "given": "Paula", "initials": "P"}, {"family": "Ragaller", "given": "Franziska", "initials": "F", "orcid": "0000-0002-4148-262X", "researcher": {"href": "https://publications.scilifelab.se/researcher/9ead87aca17f476b9febf3f020567c4b.json"}}, {"family": "Carravilla", "given": "Pablo", "initials": "P", "orcid": "0000-0001-6592-7630", "researcher": {"href": "https://publications.scilifelab.se/researcher/b791a8068a724c179b731f0446b1737a.json"}}, {"family": "Gurdap", "given": "Cenk O", "initials": "CO", "orcid": "0000-0002-5327-2523", "researcher": {"href": "https://publications.scilifelab.se/researcher/9e3e5501e8d84f46884f1001367ab262.json"}}, {"family": "Cetinkaya", "given": "Agit", "initials": "A"}, {"family": "Sezgin", "given": "Erdinc", "initials": "E", "orcid": "0000-0002-4915-388X", "researcher": {"href": "https://publications.scilifelab.se/researcher/34d3b05d68d64f698ff08dc655d2fe26.json"}}, {"family": "Klymchenko", "given": "Andrey S", "initials": "AS", "orcid": "0000-0002-2423-830X", "researcher": {"href": "https://publications.scilifelab.se/researcher/2e5109394fec48b4a38f382ad62d7d31.json"}}], "type": "posted-content", "published": "2025-10-17", "journal": {"title": "biorxiv", "issn": null, "issn-l": null, "volume": null, "issue": null, "pages": null}, "abstract": null, "doi": "10.1101/2025.10.17.683001", "pmid": null, "labels": {"Integrated Microscopy Technologies Stockholm": "Service"}, "xrefs": [], "notes": [], "created": "2025-10-21T09:38:09.353Z", "modified": "2025-12-18T19:01:18.923Z"}, {"entity": "publication", "iuid": "d2c5072fe8644c0b8b5297199b1fd0e5", "links": {"self": {"href": "https://publications.scilifelab.se/publication/d2c5072fe8644c0b8b5297199b1fd0e5.json"}, "display": {"href": "https://publications.scilifelab.se/publication/d2c5072fe8644c0b8b5297199b1fd0e5"}}, "title": "Measuring plasma membrane fluidity using confocal microscopy.", "authors": [{"family": "Carravilla", "given": "Pablo", "initials": "P", "orcid": "0000-0001-6592-7630", "researcher": {"href": "https://publications.scilifelab.se/researcher/b791a8068a724c179b731f0446b1737a.json"}}, {"family": "Andronico", "given": "Luca", "initials": "L"}, {"family": "Schlegel", "given": "Jan", "initials": "J", "orcid": "0000-0003-3159-8079", "researcher": {"href": "https://publications.scilifelab.se/researcher/c94351acc3934c3084f1ed0429a1879a.json"}}, {"family": "Urem", "given": "Yagmur B", "initials": "YB", "orcid": "0009-0008-5053-2191", "researcher": {"href": "https://publications.scilifelab.se/researcher/78c952b0962443199c9be0ce64d48e4e.json"}}, {"family": "Sjule", "given": "Ellen", "initials": "E", "orcid": "0000-0003-0166-2826", "researcher": {"href": "https://publications.scilifelab.se/researcher/4e0fd8926ef843a0a8db936df7209107.json"}}, {"family": "Ragaller", "given": "Franziska", "initials": "F", "orcid": "0000-0002-4148-262X", "researcher": {"href": "https://publications.scilifelab.se/researcher/9ead87aca17f476b9febf3f020567c4b.json"}}, {"family": "Weber", "given": "Florian", "initials": "F", "orcid": "0000-0002-8946-1377", "researcher": {"href": "https://publications.scilifelab.se/researcher/2d4b9aaf4b7041cdbf1d99f3a0d833f2.json"}}, {"family": "Gurdap", "given": "Cenk O", "initials": "CO", "orcid": "0000-0002-5327-2523", "researcher": {"href": "https://publications.scilifelab.se/researcher/9e3e5501e8d84f46884f1001367ab262.json"}}, {"family": "Ascioglu", "given": "Yavuz", "initials": "Y"}, {"family": "Sych", "given": "Taras", "initials": "T", "orcid": "0000-0002-7330-9063", "researcher": {"href": "https://publications.scilifelab.se/researcher/d104a7d8096b4a4ab2a9fe618489fc7e.json"}}, {"family": "Lorent", "given": "Joseph", "initials": "J"}, {"family": "Sezgin", "given": "Erdinc", "initials": "E", "orcid": "0000-0002-4915-388X", "researcher": {"href": "https://publications.scilifelab.se/researcher/34d3b05d68d64f698ff08dc655d2fe26.json"}}], "type": "journal article", "published": "2025-02-19", "journal": {"title": "Nat Protoc", "issn": "1750-2799", "issn-l": null}, "abstract": "Membrane fluidity is a crucial parameter for cellular physiology. Recent evidence suggests that fluidity varies between cell types and states and in diseases. As membrane fluidity has gradually become an important consideration in cell biology and biomedicine, it is essential to have reliable and quantitative ways to measure it in cells. In the past decade, there has been substantial progress both in chemical probes and in imaging tools to make membrane fluidity measurements easier and more reliable. We have recently established a robust pipeline, using confocal imaging and new environment-sensitive probes, that has been successfully used for several studies. Here we present our detailed protocol for membrane fluidity measurement, from labeling to imaging and image analysis. The protocol takes ~4 h and requires basic expertise in cell culture, wet lab and microscopy.", "doi": "10.1038/s41596-024-01122-8", "pmid": "39972239", "labels": {"Integrated Microscopy Technologies Stockholm": "Service"}, "xrefs": [{"db": "pii", "key": "10.1038/s41596-024-01122-8"}], "notes": [], "created": "2025-03-11T12:49:46.696Z", "modified": "2025-03-24T08:20:58.620Z"}, {"entity": "publication", "iuid": "149b1072f1304f8789c9949d7b997492", "links": {"self": {"href": "https://publications.scilifelab.se/publication/149b1072f1304f8789c9949d7b997492.json"}, "display": {"href": "https://publications.scilifelab.se/publication/149b1072f1304f8789c9949d7b997492"}}, "title": "Apoptosis-mediated ADAM10 activation removes a mucin barrier promoting T cell efferocytosis.", "authors": [{"family": "Drexhage", "given": "Linnea Z", "initials": "LZ", "orcid": "0000-0001-7679-4466", "researcher": {"href": "https://publications.scilifelab.se/researcher/0773a617433d46ceb0ea431f60ee59d1.json"}}, {"family": "Zhang", "given": "Shengpan", "initials": "S", "orcid": "0000-0002-1134-7101", "researcher": {"href": "https://publications.scilifelab.se/researcher/dfdfec78321d488eae1c131484d6a1f0.json"}}, {"family": "Dupont", "given": "Maeva", "initials": "M"}, {"family": "Ragaller", "given": "Franziska", "initials": "F", "orcid": "0000-0002-4148-262X", "researcher": {"href": "https://publications.scilifelab.se/researcher/9ead87aca17f476b9febf3f020567c4b.json"}}, {"family": "Sjule", "given": "Ellen", "initials": "E", "orcid": "0000-0003-0166-2826", "researcher": {"href": "https://publications.scilifelab.se/researcher/4e0fd8926ef843a0a8db936df7209107.json"}}, {"family": "Cabezas-Caballero", "given": "Jose", "initials": "J", "orcid": "0000-0001-9871-3428", "researcher": {"href": "https://publications.scilifelab.se/researcher/460c76524cf748efabf39b555b34f9ee.json"}}, {"family": "Deimel", "given": "Lachlan P", "initials": "LP", "orcid": "0000-0003-3803-871X", "researcher": {"href": "https://publications.scilifelab.se/researcher/fc322e4e39da4a769c515a5c77b844cf.json"}}, {"family": "Robertson", "given": "Helen", "initials": "H", "orcid": "0000-0002-9190-8799", "researcher": {"href": "https://publications.scilifelab.se/researcher/e1cbf72e1e184e91917ffe3208e9095c.json"}}, {"family": "Russell", "given": "Rebecca A", "initials": "RA", "orcid": "0000-0002-6517-6483", "researcher": {"href": "https://publications.scilifelab.se/researcher/7ce1e727941a4626823fc4d4db07088c.json"}}, {"family": "Dushek", "given": "Omer", "initials": "O", "orcid": "0000-0001-5847-5226", "researcher": {"href": "https://publications.scilifelab.se/researcher/444272de68ea4e5fb05ca044d92fee24.json"}}, {"family": "Sezgin", "given": "Erdinc", "initials": "E", "orcid": "0000-0002-4915-388X", "researcher": {"href": "https://publications.scilifelab.se/researcher/34d3b05d68d64f698ff08dc655d2fe26.json"}}, {"family": "Karaji", "given": "Niloofar", "initials": "N"}, {"family": "Sattentau", "given": "Quentin J", "initials": "QJ", "orcid": "0000-0001-7170-1937", "researcher": {"href": "https://publications.scilifelab.se/researcher/d78243e447114e179d1aef437cb35717.json"}}], "type": "journal article", "published": "2024-01-15", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "15", "issue": "1", "pages": "541", "issn-l": "2041-1723"}, "abstract": "Efferocytic clearance of apoptotic cells in general, and T cells in particular, is required for tissue and immune homeostasis. Transmembrane mucins are extended glycoproteins highly expressed in the cell glycocalyx that function as a barrier to phagocytosis. Whether and how mucins may be regulated during cell death to facilitate efferocytic corpse clearance is not well understood. Here we show that normal and transformed human T cells express a subset of mucins which are rapidly and selectively removed from the cell surface during apoptosis. This process is mediated by the ADAM10 sheddase, the activity of which is associated with XKR8-catalyzed flipping of phosphatidylserine to the outer leaflet of the plasma membrane. Mucin clearance enhances uptake of apoptotic T cells by macrophages, confirming mucins as an enzymatically-modulatable barrier to efferocytosis. Together these findings demonstrate a glycocalyx regulatory pathway with implications for therapeutic intervention in the clearance of normal and transformed apoptotic T cells.", "doi": "10.1038/s41467-023-44619-8", "pmid": "38225245", "labels": {"Integrated Microscopy Technologies Stockholm": "Service"}, "xrefs": [{"db": "pii", "key": "10.1038/s41467-023-44619-8"}], "notes": [], "created": "2024-01-16T06:21:10.968Z", "modified": "2024-01-16T06:21:11.640Z"}, {"entity": "publication", "iuid": "6f8b796fc932411ca4ac6f38416b8a4e", "links": {"self": {"href": "https://publications.scilifelab.se/publication/6f8b796fc932411ca4ac6f38416b8a4e.json"}, "display": {"href": "https://publications.scilifelab.se/publication/6f8b796fc932411ca4ac6f38416b8a4e"}}, "title": "Quantifying fluorescence lifetime responsiveness of environment sensitive probes for membrane fluidity measurements", "authors": [{"family": "Ragaller", "given": "Franziska", "initials": "F", "orcid": "0000-0002-4148-262X", "researcher": {"href": "https://publications.scilifelab.se/researcher/9ead87aca17f476b9febf3f020567c4b.json"}}, {"family": "Sjule", "given": "Ellen", "initials": "E", "orcid": "0000-0003-0166-2826", "researcher": {"href": "https://publications.scilifelab.se/researcher/4e0fd8926ef843a0a8db936df7209107.json"}}, {"family": "Urem", "given": "Yagmur Balim", "initials": "YB", "orcid": "0009-0008-5053-2191", "researcher": {"href": "https://publications.scilifelab.se/researcher/78c952b0962443199c9be0ce64d48e4e.json"}}, {"family": "Schlegel", "given": "Jan", "initials": "J", "orcid": "0000-0003-3159-8079", "researcher": {"href": "https://publications.scilifelab.se/researcher/c94351acc3934c3084f1ed0429a1879a.json"}}, {"family": "El", "given": "Rojbin", "initials": "R"}, {"family": "Urbancic", "given": "Dunja", "initials": "D"}, {"family": "Urbancic", "given": "Iztok", "initials": "I", "orcid": "0000-0003-3603-6585", "researcher": {"href": "https://publications.scilifelab.se/researcher/426193c07b2b4ab895595d89b8d97a0d.json"}}, {"family": "Blom", "given": "Hans", "initials": "H", "orcid": "0000-0002-5584-9170", "researcher": {"href": "https://publications.scilifelab.se/researcher/3ce356a74dc84e0ea6af85397f11d869.json"}}, {"family": "Sezgin", "given": "Erdinc", "initials": "E", "orcid": "0000-0002-4915-388X", "researcher": {"href": "https://publications.scilifelab.se/researcher/34d3b05d68d64f698ff08dc655d2fe26.json"}}], "type": "posted-content", "published": "2023-10-25", "journal": {"title": "biorxiv", "issn": null, "issn-l": null, "volume": null, "issue": null, "pages": null}, "abstract": null, "doi": "10.1101/2023.10.23.563572", "pmid": null, "labels": {"Integrated Microscopy Technologies Stockholm": "Collaborative"}, "xrefs": [], "notes": [], "created": "2024-01-10T10:29:03.392Z", "modified": "2025-12-18T19:44:49.894Z"}, {"entity": "publication", "iuid": "fb49c8197b1246479e091dce2f63137f", "links": {"self": {"href": "https://publications.scilifelab.se/publication/fb49c8197b1246479e091dce2f63137f.json"}, "display": {"href": "https://publications.scilifelab.se/publication/fb49c8197b1246479e091dce2f63137f"}}, "title": "Dissecting the mechanisms of environment sensitivity of smart probes for quantitative assessment of membrane properties.", "authors": [{"family": "Ragaller", "given": "Franziska", "initials": "F", "orcid": "0000-0002-4148-262X", "researcher": {"href": "https://publications.scilifelab.se/researcher/9ead87aca17f476b9febf3f020567c4b.json"}}, {"family": "Andronico", "given": "Luca", "initials": "L"}, {"family": "Sykora", "given": "Jan", "initials": "J"}, {"family": "Kulig", "given": "Waldemar", "initials": "W", "orcid": "0000-0001-7568-0029", "researcher": {"href": "https://publications.scilifelab.se/researcher/9ab921f1f0514764a3f95fb14e087a96.json"}}, {"family": "Rog", "given": "Tomasz", "initials": "T"}, {"family": "Urem", "given": "Yagmur Balim", "initials": "YB"}, {"family": "Danylchuk", "given": "Dmytro I", "initials": "DI"}, {"family": "Hof", "given": "Martin", "initials": "M"}, {"family": "Klymchenko", "given": "Andrey", "initials": "A"}, {"family": "Amaro", "given": "Mariana", "initials": "M"}, {"family": "Vattulainen", "given": "Ilpo", "initials": "I", "orcid": "0000-0001-7408-3214", "researcher": {"href": "https://publications.scilifelab.se/researcher/cd93a41698e64d5e9c4c1b32cad2f412.json"}}, {"family": "Sezgin", "given": "Erdinc", "initials": "E", "orcid": "0000-0002-4915-388X", "researcher": {"href": "https://publications.scilifelab.se/researcher/34d3b05d68d64f698ff08dc655d2fe26.json"}}], "type": "journal article", "published": "2022-09-00", "journal": {"title": "Open Biol", "issn": "2046-2441", "volume": "12", "issue": "9", "pages": "220175", "issn-l": "2046-2441"}, "abstract": "The plasma membrane, as a highly complex cell organelle, serves as a crucial platform for a multitude of cellular processes. Its collective biophysical properties are largely determined by the structural diversity of the different lipid species it accommodates. Therefore, a detailed investigation of biophysical properties of the plasma membrane is of utmost importance for a comprehensive understanding of biological processes occurring therein. During the past two decades, several environment-sensitive probes have been developed and become popular tools to investigate membrane properties. Although these probes are assumed to report on membrane order in similar ways, their individual mechanisms remain to be elucidated. In this study, using model membrane systems, we characterized the probes Pro12A, NR12S and NR12A in depth and examined their sensitivity to parameters with potential biological implications, such as the degree of lipid saturation, double bond position and configuration (cis versus trans), phospholipid headgroup and cholesterol content. Applying spectral imaging together with atomistic molecular dynamics simulations and time-dependent fluorescent shift analyses, we unravelled individual sensitivities of these probes to different biophysical properties, their distinct localizations and specific relaxation processes in membranes. Overall, Pro12A, NR12S and NR12A serve together as a toolbox with a wide range of applications allowing to select the most appropriate probe for each specific research question.", "doi": "10.1098/rsob.220175", "pmid": "36099931", "labels": {"Integrated Microscopy Technologies Stockholm": "Service"}, "xrefs": [], "notes": [], "created": "2022-09-16T07:58:04.554Z", "modified": "2022-09-16T07:58:04.714Z"}]}