{"entity": "researcher", "timestamp": "2026-06-15T18:08:19.976Z", "family": "Gurdap", "given": "Cenk Onur", "initials": "CO", "orcid": "0000-0002-5327-2523", "affiliations": [], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/9e3e5501e8d84f46884f1001367ab262.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/9e3e5501e8d84f46884f1001367ab262"}}, "publications": [{"entity": "publication", "iuid": "48bf48701cf44883bb1fe87945bf952b", "links": {"self": {"href": "https://publications.scilifelab.se/publication/48bf48701cf44883bb1fe87945bf952b.json"}, "display": {"href": "https://publications.scilifelab.se/publication/48bf48701cf44883bb1fe87945bf952b"}}, "title": "Prodan-based solvatochromic probes for polarity imaging of organelles", "authors": [{"family": "Aknine", "given": "Nathan", "initials": "N"}, {"family": "Holban", "given": "Paula", "initials": "P"}, {"family": "Ragaller", "given": "Franziska", "initials": "F", "orcid": "0000-0002-4148-262X", "researcher": {"href": "https://publications.scilifelab.se/researcher/9ead87aca17f476b9febf3f020567c4b.json"}}, {"family": "Carravilla", "given": "Pablo", "initials": "P", "orcid": "0000-0001-6592-7630", "researcher": {"href": "https://publications.scilifelab.se/researcher/b791a8068a724c179b731f0446b1737a.json"}}, {"family": "Gurdap", "given": "Cenk O", "initials": "CO", "orcid": "0000-0002-5327-2523", "researcher": {"href": "https://publications.scilifelab.se/researcher/9e3e5501e8d84f46884f1001367ab262.json"}}, {"family": "Cetinkaya", "given": "Agit", "initials": "A"}, {"family": "Sezgin", "given": "Erdinc", "initials": "E", "orcid": "0000-0002-4915-388X", "researcher": {"href": "https://publications.scilifelab.se/researcher/34d3b05d68d64f698ff08dc655d2fe26.json"}}, {"family": "Klymchenko", "given": "Andrey S", "initials": "AS", "orcid": "0000-0002-2423-830X", "researcher": {"href": "https://publications.scilifelab.se/researcher/2e5109394fec48b4a38f382ad62d7d31.json"}}], "type": "posted-content", "published": "2025-10-17", "journal": {"title": "biorxiv", "issn": null, "issn-l": null, "volume": null, "issue": null, "pages": null}, "abstract": null, "doi": "10.1101/2025.10.17.683001", "pmid": null, "labels": {"Integrated Microscopy Technologies Stockholm": "Service"}, "xrefs": [], "notes": [], "created": "2025-10-21T09:38:09.353Z", "modified": "2025-12-18T19:01:18.923Z"}, {"entity": "publication", "iuid": "d2c5072fe8644c0b8b5297199b1fd0e5", "links": {"self": {"href": "https://publications.scilifelab.se/publication/d2c5072fe8644c0b8b5297199b1fd0e5.json"}, "display": {"href": "https://publications.scilifelab.se/publication/d2c5072fe8644c0b8b5297199b1fd0e5"}}, "title": "Measuring plasma membrane fluidity using confocal microscopy.", "authors": [{"family": "Carravilla", "given": "Pablo", "initials": "P", "orcid": "0000-0001-6592-7630", "researcher": {"href": "https://publications.scilifelab.se/researcher/b791a8068a724c179b731f0446b1737a.json"}}, {"family": "Andronico", "given": "Luca", "initials": "L"}, {"family": "Schlegel", "given": "Jan", "initials": "J", "orcid": "0000-0003-3159-8079", "researcher": {"href": "https://publications.scilifelab.se/researcher/c94351acc3934c3084f1ed0429a1879a.json"}}, {"family": "Urem", "given": "Yagmur B", "initials": "YB", "orcid": "0009-0008-5053-2191", "researcher": {"href": "https://publications.scilifelab.se/researcher/78c952b0962443199c9be0ce64d48e4e.json"}}, {"family": "Sjule", "given": "Ellen", "initials": "E", "orcid": "0000-0003-0166-2826", "researcher": {"href": "https://publications.scilifelab.se/researcher/4e0fd8926ef843a0a8db936df7209107.json"}}, {"family": "Ragaller", "given": "Franziska", "initials": "F", "orcid": "0000-0002-4148-262X", "researcher": {"href": "https://publications.scilifelab.se/researcher/9ead87aca17f476b9febf3f020567c4b.json"}}, {"family": "Weber", "given": "Florian", "initials": "F", "orcid": "0000-0002-8946-1377", "researcher": {"href": "https://publications.scilifelab.se/researcher/2d4b9aaf4b7041cdbf1d99f3a0d833f2.json"}}, {"family": "Gurdap", "given": "Cenk O", "initials": "CO", "orcid": "0000-0002-5327-2523", "researcher": {"href": "https://publications.scilifelab.se/researcher/9e3e5501e8d84f46884f1001367ab262.json"}}, {"family": "Ascioglu", "given": "Yavuz", "initials": "Y"}, {"family": "Sych", "given": "Taras", "initials": "T", "orcid": "0000-0002-7330-9063", "researcher": {"href": "https://publications.scilifelab.se/researcher/d104a7d8096b4a4ab2a9fe618489fc7e.json"}}, {"family": "Lorent", "given": "Joseph", "initials": "J"}, {"family": "Sezgin", "given": "Erdinc", "initials": "E", "orcid": "0000-0002-4915-388X", "researcher": {"href": "https://publications.scilifelab.se/researcher/34d3b05d68d64f698ff08dc655d2fe26.json"}}], "type": "journal article", "published": "2025-02-19", "journal": {"title": "Nat Protoc", "issn": "1750-2799", "issn-l": null}, "abstract": "Membrane fluidity is a crucial parameter for cellular physiology. Recent evidence suggests that fluidity varies between cell types and states and in diseases. As membrane fluidity has gradually become an important consideration in cell biology and biomedicine, it is essential to have reliable and quantitative ways to measure it in cells. In the past decade, there has been substantial progress both in chemical probes and in imaging tools to make membrane fluidity measurements easier and more reliable. We have recently established a robust pipeline, using confocal imaging and new environment-sensitive probes, that has been successfully used for several studies. Here we present our detailed protocol for membrane fluidity measurement, from labeling to imaging and image analysis. The protocol takes ~4 h and requires basic expertise in cell culture, wet lab and microscopy.", "doi": "10.1038/s41596-024-01122-8", "pmid": "39972239", "labels": {"Integrated Microscopy Technologies Stockholm": "Service"}, "xrefs": [{"db": "pii", "key": "10.1038/s41596-024-01122-8"}], "notes": [], "created": "2025-03-11T12:49:46.696Z", "modified": "2025-03-24T08:20:58.620Z"}, {"entity": "publication", "iuid": "fb9d76d357e04f8089f321a7f617e93e", "links": {"self": {"href": "https://publications.scilifelab.se/publication/fb9d76d357e04f8089f321a7f617e93e.json"}, "display": {"href": "https://publications.scilifelab.se/publication/fb9d76d357e04f8089f321a7f617e93e"}}, "title": "High-throughput analysis of membrane order unveils a hidden dimension in immune cell states", "authors": [{"family": "Andronico", "given": "Luca A", "initials": "LA", "orcid": "0000-0002-4326-073X", "researcher": {"href": "https://publications.scilifelab.se/researcher/8818b25185e54c7e9f120d34c1fc69cd.json"}}, {"family": "Gurdap", "given": "Cenk O", "initials": "CO", "orcid": "0000-0002-5327-2523", "researcher": {"href": "https://publications.scilifelab.se/researcher/9e3e5501e8d84f46884f1001367ab262.json"}}, {"family": "Arora", "given": "Abishek", "initials": "A", "orcid": "0000-0002-6149-4417", "researcher": {"href": "https://publications.scilifelab.se/researcher/384ef4f8d6eb49d6873c87556843a7a2.json"}}, {"family": "Sandoz", "given": "Patrick A", "initials": "PA", "orcid": "0000-0002-8379-7267", "researcher": {"href": "https://publications.scilifelab.se/researcher/acd737132e4b4b378257ae8f0316b3a2.json"}}, {"family": "Jiang", "given": "Yidan", "initials": "Y"}, {"family": "Giatrellis", "given": "Sarantis", "initials": "S", "orcid": "0009-0002-6412-0530", "researcher": {"href": "https://publications.scilifelab.se/researcher/9554fd11c5a649a88d9d3cb51776012a.json"}}, {"family": "de Boer", "given": "Leon", "initials": "L"}, {"family": "Carannante", "given": "Valentina", "initials": "V"}, {"family": "Iskrak", "given": "Sofiia", "initials": "S"}, {"family": "Mikes", "given": "Jaromir", "initials": "J", "orcid": "0000-0002-9941-7855", "researcher": {"href": "https://publications.scilifelab.se/researcher/21c127bffa7c4a01af7fad8ba6bac90b.json"}}, {"family": "Buggert", "given": "Marcus", "initials": "M", "orcid": "0000-0003-0633-1719", "researcher": {"href": "https://publications.scilifelab.se/researcher/9a54e4b5136642eeafa77ac5118a0c81.json"}}, {"family": "\u00d6sterborg", "given": "Anders", "initials": "A", "orcid": "0000-0001-6489-4865", "researcher": {"href": "https://publications.scilifelab.se/researcher/4a1df5f584834eec8d7f90ab421a31ec.json"}}, {"family": "\u00d6nfelt", "given": "Bj\u00f6rn", "initials": "B", "orcid": "0000-0001-5178-7593", "researcher": {"href": "https://publications.scilifelab.se/researcher/14bcdcf94cab40ff92319869d243f5d8.json"}}, {"family": "Klymchenko", "given": "Andrey", "initials": "A", "orcid": "0000-0002-2423-830X", "researcher": {"href": "https://publications.scilifelab.se/researcher/2e5109394fec48b4a38f382ad62d7d31.json"}}, {"family": "Brodin", "given": "Petter", "initials": "P", "orcid": "0000-0002-8103-0046", "researcher": {"href": "https://publications.scilifelab.se/researcher/40097353cdb24e52bf2330eb687042bf.json"}}, {"family": "Sezgin", "given": "Erdinc", "initials": "E", "orcid": "0000-0002-4915-388X", "researcher": {"href": "https://publications.scilifelab.se/researcher/34d3b05d68d64f698ff08dc655d2fe26.json"}}], "type": "posted-content", "published": "2024-01-16", "journal": {"title": "biorxiv", "issn": null, "issn-l": null, "volume": null, "issue": null, "pages": null}, "abstract": null, "doi": "10.1101/2024.01.15.575649", "pmid": null, "labels": {"Integrated Microscopy Technologies Stockholm": "Service"}, "xrefs": [], "notes": [], "created": "2024-01-29T10:52:39.251Z", "modified": "2025-12-18T19:32:03.682Z"}, {"entity": "publication", "iuid": "86d3c36a527344fea74a8555b432baad", "links": {"self": {"href": "https://publications.scilifelab.se/publication/86d3c36a527344fea74a8555b432baad.json"}, "display": {"href": "https://publications.scilifelab.se/publication/86d3c36a527344fea74a8555b432baad"}}, "title": "How Does Liquid-Liquid Phase Separation in Model Membranes Reflect Cell Membrane Heterogeneity?", "authors": [{"family": "Sych", "given": "Taras", "initials": "T", "orcid": "0000-0002-7330-9063", "researcher": {"href": "https://publications.scilifelab.se/researcher/d104a7d8096b4a4ab2a9fe618489fc7e.json"}}, {"family": "Gurdap", "given": "Cenk Onur", "initials": "CO", "orcid": "0000-0002-5327-2523", "researcher": {"href": "https://publications.scilifelab.se/researcher/9e3e5501e8d84f46884f1001367ab262.json"}}, {"family": "Wedemann", "given": "Linda", "initials": "L"}, {"family": "Sezgin", "given": "Erdinc", "initials": "E", "orcid": "0000-0002-4915-388X", "researcher": {"href": "https://publications.scilifelab.se/researcher/34d3b05d68d64f698ff08dc655d2fe26.json"}}], "type": "journal article", "published": "2021-04-28", "journal": {"title": "Membranes", "issn": "2077-0375", "volume": "11", "issue": "5", "pages": "323", "issn-l": null}, "abstract": "Although liquid-liquid phase separation of cytoplasmic or nuclear components in cells has been a major focus in cell biology, it is only recently that the principle of phase separation has been a long-standing concept and extensively studied in biomembranes. Membrane phase separation has been reconstituted in simplified model systems, and its detailed physicochemical principles, including essential phase diagrams, have been extensively explored. These model membrane systems have proven very useful to study the heterogeneity in cellular membranes, however, concerns have been raised about how reliably they can represent native membranes. In this review, we will discuss how phase-separated membrane systems can mimic cellular membranes and where they fail to reflect the native cell membrane heterogeneity. We also include a few humble suggestions on which phase-separated systems should be used for certain applications, and which interpretations should be avoided to prevent unreliable conclusions.", "doi": "10.3390/membranes11050323", "pmid": "33925240", "labels": {"Integrated Microscopy Technologies Stockholm": "Service"}, "xrefs": [{"db": "pii", "key": "membranes11050323"}, {"db": "pmc", "key": "PMC8146956"}], "notes": [], "created": "2021-08-06T11:16:45.351Z", "modified": "2021-11-10T12:25:26.526Z"}]}