{"entity": "researcher", "timestamp": "2026-07-20T22:04:41.804Z", "family": "Hennig", "given": "Lars", "initials": "L", "orcid": "0000-0002-6645-1862", "affiliations": ["Department of Plant Biology, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, Uppsala, 75007, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/9b350e5d9ba74c27bf1709ff0457e605.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/9b350e5d9ba74c27bf1709ff0457e605"}}, "publications": [{"entity": "publication", "iuid": "984a5218407a416aa006a0f62bb5521b", "links": {"self": {"href": "https://publications.scilifelab.se/publication/984a5218407a416aa006a0f62bb5521b.json"}, "display": {"href": "https://publications.scilifelab.se/publication/984a5218407a416aa006a0f62bb5521b"}}, "title": "Role of H1 and DNA methylation in selective regulation of transposable elements during heat stress.", "authors": [{"family": "Liu", "given": "Shujing", "initials": "S", "orcid": "0000-0002-5783-4204", "researcher": {"href": "https://publications.scilifelab.se/researcher/4a1916bba339494f995b0a7a84428fcf.json"}}, {"family": "de Jonge", "given": "Jennifer", "initials": "J"}, {"family": "Trejo-Arellano", "given": "Minerva S", "initials": "MS", "orcid": "0000-0002-1982-3475", "researcher": {"href": "https://publications.scilifelab.se/researcher/0d1fc0e7004b41de889d2223941385c7.json"}}, {"family": "Santos-Gonz\u00e1lez", "given": "Juan", "initials": "J"}, {"family": "K\u00f6hler", "given": "Claudia", "initials": "C", "orcid": "0000-0002-2619-4857", "researcher": {"href": "https://publications.scilifelab.se/researcher/accd3f9307614c8ab67154dd5e50cdac.json"}}, {"family": "Hennig", "given": "Lars", "initials": "L", "orcid": "0000-0002-6645-1862", "researcher": {"href": "https://publications.scilifelab.se/researcher/9b350e5d9ba74c27bf1709ff0457e605.json"}}], "type": "journal article", "published": "2021-02-00", "journal": {"title": "New Phytol.", "issn": "1469-8137", "volume": "229", "issue": "4", "pages": "2238-2250", "issn-l": "0028-646X"}, "abstract": "Heat-stressed Arabidopsis plants release heterochromatin-associated transposable element (TE) silencing, yet it is not accompanied by major reductions of epigenetic repressive modifications. In this study, we explored the functional role of histone H1 in repressing heterochromatic TEs in response to heat stress. We generated and analyzed RNA and bisulfite-sequencing data of wild-type and h1 mutant seedlings before and after heat stress. Loss of H1 caused activation of pericentromeric Gypsy elements upon heat treatment, despite these elements remaining highly methylated. By contrast, nonpericentromeric Copia elements became activated concomitantly with loss of DNA methylation. The same Copia elements became activated in heat-treated chromomethylase 2 (cmt2) mutants, indicating that H1 represses Copia elements through maintaining DNA methylation under heat. We discovered that H1 is required for TE repression in response to heat stress, but its functional role differs depending on TE location. Strikingly, H1-deficient plants treated with the DNA methyltransferase inhibitor zebularine were highly tolerant to heat stress, suggesting that both H1 and DNA methylation redundantly suppress the plant response to heat stress.", "doi": "10.1111/nph.17018", "pmid": "33091182", "labels": {"National Genomics Infrastructure": "Service", "NGI Uppsala (SNP&SEQ Technology Platform)": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC7894476"}], "notes": [], "created": "2020-12-08T23:37:01.805Z", "modified": "2021-11-10T12:27:17.083Z"}, {"entity": "publication", "iuid": "3d6084989c4c436bb04585ec88b5f5f5", "links": {"self": {"href": "https://publications.scilifelab.se/publication/3d6084989c4c436bb04585ec88b5f5f5.json"}, "display": {"href": "https://publications.scilifelab.se/publication/3d6084989c4c436bb04585ec88b5f5f5"}}, "title": "Polycomb Repressive Complex 2-mediated histone modification H3K27me3 is associated with embryogenic potential in Norway spruce.", "authors": [{"family": "Nakamura", "given": "Miyuki", "initials": "M", "orcid": "0000-0002-8153-7293", "researcher": {"href": "https://publications.scilifelab.se/researcher/fa9f227109b54fa9a1457a1f7af197af.json"}}, {"family": "Batista", "given": "Rita A", "initials": "RA"}, {"family": "K\u00f6hler", "given": "Claudia", "initials": "C", "orcid": "0000-0002-2619-4857", "researcher": {"href": "https://publications.scilifelab.se/researcher/accd3f9307614c8ab67154dd5e50cdac.json"}}, {"family": "Hennig", "given": "Lars", "initials": "L", "orcid": "0000-0002-6645-1862", "researcher": {"href": "https://publications.scilifelab.se/researcher/9b350e5d9ba74c27bf1709ff0457e605.json"}}], "type": "journal article", "published": "2020-10-22", "journal": {"title": "J. Exp. Bot.", "issn": "1460-2431", "volume": "71", "issue": "20", "pages": "6366-6378", "issn-l": "0022-0957"}, "abstract": "Epigenetic reprogramming during germ cell formation is essential to gain pluripotency and thus embryogenic potential. The histone modification H3K27me3, which is catalysed by the Polycomb repressive complex 2 (PRC2), regulates important developmental processes in both plants and animals, and defects in PRC2 components cause pleiotropic developmental abnormalities. Nevertheless, the role of H3K27me3 in determining embryogenic potential in gymnosperms is still elusive. To address this, we generated H3K27me3 profiles of Norway spruce (Picea abies) embryonic callus and non-embryogenic callus using CUT&RUN, which is a powerful method for chromatin profiling. Here, we show that H3K27me3 mainly accumulated in genic regions in the Norway spruce genome, similarly to what is observed in other plant species. Interestingly, H3K27me3 levels in embryonic callus were much lower than those in the other examined tissues, but markedly increased upon embryo induction. These results show that H3K27me3 levels are associated with the embryogenic potential of a given tissue, and that the early phase of somatic embryogenesis is accompanied by changes in H3K27me3 levels. Thus, our study provides novel insights into the role of this epigenetic mark in spruce embryogenesis and reinforces the importance of PRC2 as a key regulator of cell fate determination across different plant species.", "doi": "10.1093/jxb/eraa365", "pmid": "32894759", "labels": {"National Genomics Infrastructure": "Service", "NGI Uppsala (SNP&SEQ Technology Platform)": "Service"}, "xrefs": [{"db": "pii", "key": "5902563"}, {"db": "pmc", "key": "PMC7586741"}], "notes": [], "created": "2020-11-16T09:34:27.793Z", "modified": "2021-11-10T12:46:16.725Z"}]}