{"entity": "researcher", "timestamp": "2026-08-11T14:14:56.856Z", "family": "Sdougkou", "given": "Kalliroi", "initials": "K", "orcid": "0000-0001-9463-655X", "affiliations": [], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/975451f91bf44300a7e4b0dbb91ea401.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/975451f91bf44300a7e4b0dbb91ea401"}}, "publications": [{"entity": "publication", "iuid": "cbe37784889b4f2fa20d782492f9f6d6", "links": {"self": {"href": "https://publications.scilifelab.se/publication/cbe37784889b4f2fa20d782492f9f6d6.json"}, "display": {"href": "https://publications.scilifelab.se/publication/cbe37784889b4f2fa20d782492f9f6d6"}}, "title": "Chemical exposomics in biobanked plasma samples and associations with breast cancer risk factors", "authors": [{"family": "Edlund", "given": "Jessica", "initials": "J"}, {"family": "Sdougkou", "given": "Kalliroi", "initials": "K", "orcid": "0000-0001-9463-655X", "researcher": {"href": "https://publications.scilifelab.se/researcher/975451f91bf44300a7e4b0dbb91ea401.json"}}, {"family": "Papazian", "given": "Stefano", "initials": "S", "orcid": "0000-0003-2538-8702", "researcher": {"href": "https://publications.scilifelab.se/researcher/5de990c59ec4460e92c414dbc0ed0b16.json"}}, {"family": "Wu", "given": "Wendy Yi Ying", "initials": "WYY"}, {"family": "Martin", "given": "Jonathan W", "initials": "JW", "orcid": "0000-0001-6265-4294", "researcher": {"href": "https://publications.scilifelab.se/researcher/f275a68856cb459ebbc933b18c3e315d.json"}}, {"family": "Harlid", "given": "Sophia", "initials": "S", "orcid": "0000-0001-8540-6891", "researcher": {"href": "https://publications.scilifelab.se/researcher/76a824f2687b460890ae6d9ef40d97c9.json"}}], "type": "journal-article", "published": "2025-07-00", "journal": {"title": "J Expo Sci Environ Epidemiol", "issn": "1559-064X", "issn-l": null, "volume": "35", "issue": "4", "pages": "567-577"}, "abstract": "The chemical exposome includes exposure to numerous environmental and endogenous molecules, many of which have been linked to reproductive outcomes due to their endocrine-disrupting properties. As several breast cancer risk factors, including age and parity, are related to reproduction, it is imperative to investigate the interplay between such factors and the chemical exposome prior to conducting large scale exposome-based breast cancer studies.\n\nThis pilot study aimed to provide an overview of the chemical exposome in plasma samples from healthy women and identify associations between environmental exposures and three risk factors for breast cancer: age, parity, and age at menarche.\n\nPlasma samples (n = 161), were selected based on reproductive history from 100 women participating in the Northern Sweden Health and Disease Study, between 1987 and 2006. Samples were analyzed by liquid chromatography high-resolution mass spectrometry (LC-HRMS) for 77 priority target analytes including contaminants and hormones, with simultaneous untargeted profiling of the chemical exposome and metabolome. Linear mixed effects models were applied to test associations between risk factors and chemical levels.\n\nFifty-five target analytes were detected in at least one individual and over 94,000 untargeted features were detected across all samples. Among untargeted features, 430 could be annotated and were broadly classified as environmental (246), endogenous (167) or ambiguous (17). Applying mixed effect models to features detected in at least 70% of the samples (16,778), we found seven targeted analytes (including caffeine and various per- and poly-fluoroalkyl substances) and 38 untargeted features, positively associated with age. The directionality of these associations reversed for parity, decreasing with increasing births. Seven separate targeted analytes were associated with age at menarche.\n\nThis study demonstrates how a comprehensive chemical exposome approach can be used to inform future research prioritization regarding associations between known and unknown substances, reproduction, and breast cancer risk.\n\nThis study illustrates how chemical exposomics of long-term stored blood samples offers valuable insights to discover chemical exposures and their potential links to disease in humans, particularly those related to reproduction and breast cancer risk factors.", "doi": "10.1038/s41370-024-00736-0", "pmid": "39643621", "labels": {"Exposomics": "Collaborative"}, "xrefs": [{"db": "pii", "key": "10.1038/s41370-024-00736-0"}], "notes": [], "created": "2024-12-09T08:15:03.382Z", "modified": "2025-09-12T11:18:28.103Z"}, {"entity": "publication", "iuid": "a33f84c19445476ea4ddd447d928debc", "links": {"self": {"href": "https://publications.scilifelab.se/publication/a33f84c19445476ea4ddd447d928debc.json"}, "display": {"href": "https://publications.scilifelab.se/publication/a33f84c19445476ea4ddd447d928debc"}}, "title": "Chemical Exposomics in Human Plasma by Lipid Removal and Large-Volume Injection Gas Chromatography\u2013High-Resolution Mass Spectrometry", "authors": [{"family": "Xie", "given": "Hongyu", "initials": "H", "orcid": "0000-0002-2422-0492", "researcher": {"href": "https://publications.scilifelab.se/researcher/e038d701867b464f87f94df18e108b42.json"}}, {"family": "Sdougkou", "given": "Kalliroi", "initials": "K", "orcid": "0000-0001-9463-655X", "researcher": {"href": "https://publications.scilifelab.se/researcher/975451f91bf44300a7e4b0dbb91ea401.json"}}, {"family": "Bonnefille", "given": "B\u00e9nilde", "initials": "B", "orcid": "0000-0001-5141-7111", "researcher": {"href": "https://publications.scilifelab.se/researcher/eb5cae7e57b1443a8b296824dc186b04.json"}}, {"family": "Papazian", "given": "Stefano", "initials": "S", "orcid": "0000-0003-2538-8702", "researcher": {"href": "https://publications.scilifelab.se/researcher/5de990c59ec4460e92c414dbc0ed0b16.json"}}, {"family": "Bergdahl", "given": "Ingvar A", "initials": "IA", "orcid": "0000-0003-1227-6859", "researcher": {"href": "https://publications.scilifelab.se/researcher/dac737f9a94e49e5b4edbbd540ef1be4.json"}}, {"family": "Rantakokko", "given": "Panu", "initials": "P"}, {"family": "Martin", "given": "Jonathan W", "initials": "JW", "orcid": "0000-0001-6265-4294", "researcher": {"href": "https://publications.scilifelab.se/researcher/f275a68856cb459ebbc933b18c3e315d.json"}}], "type": "journal-article", "published": "2024-10-08", "journal": {"title": "Environ. Sci. Technol.", "issn": "0013-936X", "volume": "58", "issue": "40", "pages": "17592-17605", "issn-l": null}, "abstract": null, "doi": "10.1021/acs.est.4c05942", "pmid": null, "labels": {"Exposomics": "Technology development"}, "xrefs": [], "notes": [], "created": "2024-09-25T08:26:23.102Z", "modified": "2024-12-09T08:21:15.255Z"}, {"entity": "publication", "iuid": "365acf895bc04a4f8634782cc4578d55", "links": {"self": {"href": "https://publications.scilifelab.se/publication/365acf895bc04a4f8634782cc4578d55.json"}, "display": {"href": "https://publications.scilifelab.se/publication/365acf895bc04a4f8634782cc4578d55"}}, "title": "Longitudinal Exposomics in a Multiomic Wellness Cohort Reveals Distinctive and Dynamic Environmental Chemical Mixtures in Blood.", "authors": [{"family": "Sdougkou", "given": "Kalliroi", "initials": "K", "orcid": "0000-0001-9463-655X", "researcher": {"href": "https://publications.scilifelab.se/researcher/975451f91bf44300a7e4b0dbb91ea401.json"}}, {"family": "Papazian", "given": "Stefano", "initials": "S", "orcid": "0000-0003-2538-8702", "researcher": {"href": "https://publications.scilifelab.se/researcher/5de990c59ec4460e92c414dbc0ed0b16.json"}}, {"family": "Bonnefille", "given": "B\u00e9nilde", "initials": "B", "orcid": "0000-0001-5141-7111", "researcher": {"href": "https://publications.scilifelab.se/researcher/eb5cae7e57b1443a8b296824dc186b04.json"}}, {"family": "Xie", "given": "Hongyu", "initials": "H", "orcid": "0000-0002-2422-0492", "researcher": {"href": "https://publications.scilifelab.se/researcher/e038d701867b464f87f94df18e108b42.json"}}, {"family": "Edfors", "given": "Fredrik", "initials": "F"}, {"family": "Fagerberg", "given": "Linn", "initials": "L"}, {"family": "Uhl\u00e9n", "given": "Mathias", "initials": "M"}, {"family": "Bergstr\u00f6m", "given": "G\u00f6ran", "initials": "G"}, {"family": "Martin", "given": "Leah J", "initials": "LJ"}, {"family": "Martin", "given": "Jonathan W", "initials": "JW", "orcid": "0000-0001-6265-4294", "researcher": {"href": "https://publications.scilifelab.se/researcher/f275a68856cb459ebbc933b18c3e315d.json"}}], "type": "journal article", "published": "2024-09-17", "journal": {"title": "Environ. Sci. Technol.", "issn": "1520-5851", "volume": "58", "issue": "37", "pages": "16302-16315", "issn-l": "0013-936X"}, "abstract": "Chemical exposomes can now be comprehensively measured in human blood, but knowledge of their variability and longitudinal stability is required for robust application in cohort studies. Here, we applied high-resolution chemical exposomics to plasma of 46 adults, each sampled 6 times over 2 years in a multiomic cohort, resulting in 276 individual exposomes. In addition to quantitative analysis of 83 priority target analytes, we discovered and semiquantified substances that have rarely or never been reported in humans, including personal care products, pesticide transformation products, and polymer additives. Hierarchical cluster analysis for 519 confidently annotated substances revealed unique and distinctive coexposures, including clustered pesticides, poly(ethylene glycols), chlorinated phenols, or natural substances from tea and coffee; interactive heatmaps were publicly deposited to support open exploration of the complex (meta)data. Intraclass correlation coefficients (ICC) for all annotated substances demonstrated the relatively low stability of the exposome compared to that of proteome, microbiome, and endogenous small molecules. Implications are that the chemical exposome must be measured more frequently than other omics in longitudinal studies and four longitudinal exposure types are defined that can be considered in study design. In this small cohort, mixed-effect models nevertheless revealed significant associations between testosterone and perfluoroalkyl substances, demonstrating great potential for longitudinal exposomics in precision health research.", "doi": "10.1021/acs.est.4c05235", "pmid": "39236221", "labels": {"Exposomics": "Collaborative"}, "xrefs": [], "notes": [], "created": "2024-09-19T13:35:28.253Z", "modified": "2024-09-19T13:35:28.870Z"}, {"entity": "publication", "iuid": "98a6c5b3b3f64f71a494554a11926e35", "links": {"self": {"href": "https://publications.scilifelab.se/publication/98a6c5b3b3f64f71a494554a11926e35.json"}, "display": {"href": "https://publications.scilifelab.se/publication/98a6c5b3b3f64f71a494554a11926e35"}}, "title": "Phospholipid Removal for Enhanced Chemical Exposomics in Human Plasma", "authors": [{"family": "Sdougkou", "given": "Kalliroi", "initials": "K", "orcid": "0000-0001-9463-655X", "researcher": {"href": "https://publications.scilifelab.se/researcher/975451f91bf44300a7e4b0dbb91ea401.json"}}, {"family": "Xie", "given": "Hongyu", "initials": "H", "orcid": "0000-0002-2422-0492", "researcher": {"href": "https://publications.scilifelab.se/researcher/e038d701867b464f87f94df18e108b42.json"}}, {"family": "Papazian", "given": "Stefano", "initials": "S", "orcid": "0000-0003-2538-8702", "researcher": {"href": "https://publications.scilifelab.se/researcher/5de990c59ec4460e92c414dbc0ed0b16.json"}}, {"family": "Bonnefille", "given": "B\u00e9nilde", "initials": "B", "orcid": "0000-0001-5141-7111", "researcher": {"href": "https://publications.scilifelab.se/researcher/eb5cae7e57b1443a8b296824dc186b04.json"}}, {"family": "Bergdahl", "given": "Ingvar A", "initials": "IA"}, {"family": "Martin", "given": "Jonathan W", "initials": "JW", "orcid": "0000-0001-6265-4294", "researcher": {"href": "https://publications.scilifelab.se/researcher/f275a68856cb459ebbc933b18c3e315d.json"}}], "type": "journal-article", "published": "2023-07-18", "journal": {"title": "Environ. Sci. Technol.", "issn": "0013-936X", "issn-l": null, "volume": "57", "issue": "28", "pages": "10173-10184"}, "abstract": "The challenge of chemical exposomics in human plasma is the 1000-fold concentration gap between endogenous substances and environmental pollutants. Phospholipids are the major endogenous small molecules in plasma, thus we validated a chemical exposomics protocol with an optimized phospholipid-removal step prior to targeted and non-targeted liquid chromatography high-resolution mass spectrometry. Increased injection volume with negligible matrix effect permitted sensitive multiclass targeted analysis of 77 priority analytes; median MLOQ = 0.05 ng/mL for 200 \u03bcL plasma. In non-targeted acquisition, mean total signal intensities of non-phospholipids were enhanced 6-fold in positive (max 28-fold) and 4-fold in negative mode (max 58-fold) compared to a control method without phospholipid removal. Moreover, 109 and 28% more non-phospholipid molecular features were detected by exposomics in positive and negative mode, respectively, allowing new substances to be annotated that were non-detectable without phospholipid removal. In individual adult plasma (100 \u03bcL, n = 34), 28 analytes were detected and quantified among 10 chemical classes, and quantitation of per- and polyfluoroalkyl substances (PFAS) was externally validated by independent targeted analysis. Retrospective discovery and semi-quantification of PFAS-precursors was demonstrated, and widespread fenuron exposure is reported in plasma for the first time. The new exposomics method is complementary to metabolomics protocols, relies on open science resources, and can be scaled to support large studies of the exposome.", "doi": "10.1021/acs.est.3c00663", "pmid": "37394749", "labels": {"Exposomics": "Technology development"}, "xrefs": [], "notes": [], "created": "2023-07-04T11:51:43.597Z", "modified": "2024-01-15T08:39:22.950Z"}, {"entity": "publication", "iuid": "3cdb7e5e0b4f40fca5fcd809ed682f9e", "links": {"self": {"href": "https://publications.scilifelab.se/publication/3cdb7e5e0b4f40fca5fcd809ed682f9e.json"}, "display": {"href": "https://publications.scilifelab.se/publication/3cdb7e5e0b4f40fca5fcd809ed682f9e"}}, "title": "Nontarget mass spectrometry and in silico molecular characterization of air pollution from the Indian subcontinent", "authors": [{"family": "Papazian", "given": "Stefano", "initials": "S", "orcid": "0000-0003-2538-8702", "researcher": {"href": "https://publications.scilifelab.se/researcher/5de990c59ec4460e92c414dbc0ed0b16.json"}}, {"family": "D\u2019Agostino", "given": "Lisa A", "initials": "LA"}, {"family": "Sadiktsis", "given": "Ioannis", "initials": "I", "orcid": "0000-0002-1598-7093", "researcher": {"href": "https://publications.scilifelab.se/researcher/9e5bcda95f214e099a0c10e50884ad51.json"}}, {"family": "Froment", "given": "Jean", "initials": "J", "orcid": "0000-0003-4844-2919", "researcher": {"href": "https://publications.scilifelab.se/researcher/6322367545114b6bac92761a16065e1b.json"}}, {"family": "Bonnefille", "given": "B\u00e9nilde", "initials": "B", "orcid": "0000-0001-5141-7111", "researcher": {"href": "https://publications.scilifelab.se/researcher/eb5cae7e57b1443a8b296824dc186b04.json"}}, {"family": "Sdougkou", "given": "Kalliroi", "initials": "K", "orcid": "0000-0001-9463-655X", "researcher": {"href": "https://publications.scilifelab.se/researcher/975451f91bf44300a7e4b0dbb91ea401.json"}}, {"family": "Xie", "given": "Hongyu", "initials": "H"}, {"family": "Athanassiadis", "given": "Ioannis", "initials": "I", "orcid": "0000-0001-7069-3330", "researcher": {"href": "https://publications.scilifelab.se/researcher/708b8f152b7a4c1093e1ac83792a1419.json"}}, {"family": "Budhavant", "given": "Krishnakant", "initials": "K"}, {"family": "Dasari", "given": "Sanjeev", "initials": "S"}, {"family": "Andersson", "given": "August", "initials": "A", "orcid": "0000-0002-4659-7055", "researcher": {"href": "https://publications.scilifelab.se/researcher/036822bd7b694a8d91650b9276721d35.json"}}, {"family": "Gustafsson", "given": "\u00d6rjan", "initials": "\u00d6"}, {"family": "Martin", "given": "Jonathan W", "initials": "JW", "orcid": "0000-0001-6265-4294", "researcher": {"href": "https://publications.scilifelab.se/researcher/f275a68856cb459ebbc933b18c3e315d.json"}}], "type": "journal-article", "published": "2022-02-18", "journal": {"title": "Commun Earth Environ", "issn": "2662-4435", "volume": "3", "issue": "1", "issn-l": null}, "abstract": null, "doi": "10.1038/s43247-022-00365-1", "pmid": null, "labels": {"Exposomics": "Technology development"}, "xrefs": [], "notes": [], "created": "2023-05-23T13:49:43.962Z", "modified": "2023-06-19T13:41:34.747Z"}]}