{"entity": "researcher", "timestamp": "2026-10-05T21:16:20.011Z", "family": "Marchalot", "given": "Anne", "initials": "A", "orcid": "0000-0002-3042-8206", "affiliations": ["Center for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institutet, Stockholm, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/95b674db5dc349eaa4438563f2256290.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/95b674db5dc349eaa4438563f2256290"}}, "publications": [{"entity": "publication", "iuid": "853fcd087cf147129106a699a63b80ef", "links": {"self": {"href": "https://publications.scilifelab.se/publication/853fcd087cf147129106a699a63b80ef.json"}, "display": {"href": "https://publications.scilifelab.se/publication/853fcd087cf147129106a699a63b80ef"}}, "title": "Tumor-infiltrating immature innate lymphoid cells in colorectal cancer are biased toward ILC1/tissue-resident NK cell differentiation.", "authors": [{"family": "Marchalot", "given": "Anne", "initials": "A", "orcid": "0000-0002-3042-8206", "researcher": {"href": "https://publications.scilifelab.se/researcher/95b674db5dc349eaa4438563f2256290.json"}}, {"family": "Ljunggren", "given": "Malin", "initials": "M"}, {"family": "Stamper", "given": "Christopher", "initials": "C"}, {"family": "Weigel", "given": "Whitney", "initials": "W"}, {"family": "Tibbitt", "given": "Christopher Andrew", "initials": "CA"}, {"family": "Meininger", "given": "Isabel", "initials": "I"}, {"family": "Pandey", "given": "Ram Vinay", "initials": "RV"}, {"family": "Franklin", "given": "Miriam", "initials": "M", "orcid": "0000-0002-9402-9976", "researcher": {"href": "https://publications.scilifelab.se/researcher/b64100c086a644b8b1b7dd56f4f09da5.json"}}, {"family": "Bassett", "given": "John Washington", "initials": "JW"}, {"family": "Wirth", "given": "Lorenz", "initials": "L"}, {"family": "Colorectal Study Group", "given": "", "initials": ""}, {"family": "Lindforss", "given": "Ulrik", "initials": "U"}, {"family": "Jansson-Palmer", "given": "Gabriella", "initials": "G"}, {"family": "Nordenvall", "given": "Caroline", "initials": "C"}, {"family": "Mj\u00f6sberg", "given": "Jenny", "initials": "J", "orcid": "0000-0002-1119-0976", "researcher": {"href": "https://publications.scilifelab.se/researcher/fcca878a7f314944bf1a4290cfd5d71d.json"}}], "type": "journal article", "published": "2026-03-27", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "17", "issue": "1", "issn-l": "2041-1723"}, "abstract": "Peritoneal metastases (PM) occur in 10% of patients with colorectal cancer (CRC) and are linked to poor outcomes. Although dysregulated innate lymphoid cells (ILC) have been described in CRC, their function in CRC-PM remains unclear. Here, we analyze tumor samples from CRC and CRC-PM patients using single-cell RNA sequencing (11 patients), flow cytometry (8 patients) and differentiation assays (24 patients). Healthy colon, primary CRC and CRC-PM tumors are infiltrated by heterogeneous populations of ILC3, ILC2, ILC1, tissue resident (tr)NK cells and conventional (c)NK cells. Compared to healthy colons, primary CRC and CRC-PM tumors are depleted of ILC3 but enriched for ILC1, trNK cells and cNK cells. CRC and CRC-PM tumors harbor two immature ILC populations, early NK and na\u00efve (n)ILC, with nILCs being transcriptionally skewed toward ILC1 and trNK cells. Indeed, co-culture of isolated nILCs with OP9-DL1 cells induces intratumoral nILC differentiation into ILC1/trNK-like cells. These findings help understand the immune pathogenesis of CRC and CRC-PM and provide insights for future ILC1 and NK cell-based therapies.", "doi": "10.1038/s41467-026-71085-9", "pmid": "41896575", "labels": {"NGI Short read": "Service", "NGI Stockholm (Genomics Production)": "Service", "National Genomics Infrastructure": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC13035902"}, {"db": "pii", "key": "10.1038/s41467-026-71085-9"}], "notes": [], "created": "2026-04-10T12:22:32.013Z", "modified": "2026-04-10T12:22:32.241Z"}, {"entity": "publication", "iuid": "09be553050f342ae982025de836a0afd", "links": {"self": {"href": "https://publications.scilifelab.se/publication/09be553050f342ae982025de836a0afd.json"}, "display": {"href": "https://publications.scilifelab.se/publication/09be553050f342ae982025de836a0afd"}}, "title": "Tissue origin and virus specificity shape human CD8+ T cell cytotoxicity.", "authors": [{"family": "Niessl", "given": "Julia", "initials": "J", "orcid": "0000-0001-8852-1924", "researcher": {"href": "https://publications.scilifelab.se/researcher/710f1dd1e63e45d39a11d52eb21a5e88.json"}}, {"family": "M\u00fcller", "given": "Thomas R", "initials": "TR", "orcid": "0000-0001-5331-5522", "researcher": {"href": "https://publications.scilifelab.se/researcher/b411684ce1eb401fb8917def9c2a75a0.json"}}, {"family": "Constantz", "given": "Christian", "initials": "C"}, {"family": "Cai", "given": "Curtis", "initials": "C", "orcid": "0000-0002-3490-4361", "researcher": {"href": "https://publications.scilifelab.se/researcher/0ea25e71e96246d89437270a12cc9e47.json"}}, {"family": "Nils\u00e9n", "given": "Vera", "initials": "V"}, {"family": "Rivera Ballesteros", "given": "Olga", "initials": "O", "orcid": "0000-0001-6949-4270", "researcher": {"href": "https://publications.scilifelab.se/researcher/8b48f38b946d4fbc9d687da130f2e661.json"}}, {"family": "Adamo", "given": "Sarah", "initials": "S", "orcid": "0000-0002-6161-3156", "researcher": {"href": "https://publications.scilifelab.se/researcher/dbdfb6f121014c29a448232b60f32585.json"}}, {"family": "Kammann", "given": "Tobias", "initials": "T", "orcid": "0000-0002-8433-036X", "researcher": {"href": "https://publications.scilifelab.se/researcher/ead9a21a35e34fd092774ae13a211255.json"}}, {"family": "Mouchtaridi", "given": "Elli", "initials": "E"}, {"family": "Gao", "given": "Yu", "initials": "Y"}, {"family": "Akhirunnesa", "given": "Mily", "initials": "M", "orcid": "0000-0003-3136-9284", "researcher": {"href": "https://publications.scilifelab.se/researcher/fbce6754eaea4ebfa2437f9572f180ae.json"}}, {"family": "Raineri", "given": "Elisa J M", "initials": "EJM", "orcid": "0000-0002-5468-276X", "researcher": {"href": "https://publications.scilifelab.se/researcher/6fe9b94230d5498f8550c7ad897254e9.json"}}, {"family": "Weigel", "given": "Whitney", "initials": "W"}, {"family": "Kokkinou", "given": "Efthymia", "initials": "E"}, {"family": "Stamper", "given": "Christopher", "initials": "C", "orcid": "0000-0002-5568-8880", "researcher": {"href": "https://publications.scilifelab.se/researcher/19710b0b30ca436ca1d29bc89a376ddc.json"}}, {"family": "Marchalot", "given": "Anne", "initials": "A", "orcid": "0000-0002-3042-8206", "researcher": {"href": "https://publications.scilifelab.se/researcher/95b674db5dc349eaa4438563f2256290.json"}}, {"family": "Bassett", "given": "John", "initials": "J", "orcid": "0009-0002-5785-5088", "researcher": {"href": "https://publications.scilifelab.se/researcher/5cc9841bc436476492288239d3bb0fff.json"}}, {"family": "Ferreira", "given": "Sabrina", "initials": "S"}, {"family": "Rodahl", "given": "Inga", "initials": "I"}, {"family": "Wild", "given": "Nicole", "initials": "N", "orcid": "0000-0002-4009-473X", "researcher": {"href": "https://publications.scilifelab.se/researcher/17325f3df80248f7a1b4985094a86d7c.json"}}, {"family": "Stellaccio", "given": "Teresa", "initials": "T", "orcid": "0009-0002-5224-0893", "researcher": {"href": "https://publications.scilifelab.se/researcher/ddfda0141dd948a6b1cd6289b2c5e7f2.json"}}, {"family": "Brownlie", "given": "Demi", "initials": "D", "orcid": "0000-0001-5932-6425", "researcher": {"href": "https://publications.scilifelab.se/researcher/4e879a6ef8ff45bea049986707480e99.json"}}, {"family": "Ringqvist", "given": "Emma", "initials": "E", "orcid": "0000-0001-6303-6076", "researcher": {"href": "https://publications.scilifelab.se/researcher/cfad17442063471cb570d5be1c4dbc48.json"}}, {"family": "Flodstr\u00f6m-Tullberg", "given": "Malin", "initials": "M", "orcid": "0000-0003-2685-2052", "researcher": {"href": "https://publications.scilifelab.se/researcher/bb6d04afbe8141c2b9297854de64dab8.json"}}, {"family": "Llewellyn-Lacey", "given": "Sian", "initials": "S", "orcid": "0000-0002-4703-0413", "researcher": {"href": "https://publications.scilifelab.se/researcher/1a952db761ab4e66b251f69c00d14990.json"}}, {"family": "Tibbitt", "given": "Chris", "initials": "C", "orcid": "0000-0002-7730-7700", "researcher": {"href": "https://publications.scilifelab.se/researcher/6d8a6804b0be4aba9a9256dd2df71656.json"}}, {"family": "Hammer", "given": "Quirin", "initials": "Q", "orcid": "0000-0003-2968-6061", "researcher": {"href": "https://publications.scilifelab.se/researcher/3b6b2e5256c8401f83c3f89b5c5bb92f.json"}}, {"family": "Micha\u00eblsson", "given": "Jakob", "initials": "J"}, {"family": "Price", "given": "David A", "initials": "DA", "orcid": "0000-0001-9416-2737", "researcher": {"href": "https://publications.scilifelab.se/researcher/2dd5567af7984233b164e6906d4daaca.json"}}, {"family": "Mj\u00f6sberg", "given": "Jenny", "initials": "J", "orcid": "0000-0002-1119-0976", "researcher": {"href": "https://publications.scilifelab.se/researcher/fcca878a7f314944bf1a4290cfd5d71d.json"}}, {"family": "Marquardt", "given": "Nicole", "initials": "N", "orcid": "0000-0003-3186-4752", "researcher": {"href": "https://publications.scilifelab.se/researcher/b87ccad53d354bdd80af490dc8a0c019.json"}}, {"family": "Sandberg", "given": "Johan K", "initials": "JK", "orcid": "0000-0002-6275-0750", "researcher": {"href": "https://publications.scilifelab.se/researcher/7468c415a46645a3a4c3d28badcff954.json"}}, {"family": "Sekine", "given": "Takuya", "initials": "T", "orcid": "0000-0001-7649-0593", "researcher": {"href": "https://publications.scilifelab.se/researcher/42434c4f0f504dcd82e5830a727d5a04.json"}}, {"family": "Jorns", "given": "Carl", "initials": "C", "orcid": "0000-0001-7727-8113", "researcher": {"href": "https://publications.scilifelab.se/researcher/59d387bd893f45bdb3663a0ef3aae88a.json"}}, {"family": "Buggert", "given": "Marcus", "initials": "M", "orcid": "0000-0003-0633-1719", "researcher": {"href": "https://publications.scilifelab.se/researcher/9a54e4b5136642eeafa77ac5118a0c81.json"}}], "type": "journal article", "published": "2025-07-18", "journal": {"title": "Sci Immunol", "issn": "2470-9468", "volume": "10", "issue": "109", "pages": "eadq4881", "issn-l": null}, "abstract": "CD8+ T cells are classically defined by cytotoxic activity, but it has remained unclear whether cytotoxic programs are compartmentalized across tissues and memory subsets. Here, we established a human organ donor cohort and found that expression of conventional cytotoxic molecules-granulysin, perforin, and granzyme B-was most prominent among circulating memory CD8+ T cells and decreased progressively with tissue residency, inversely mirroring the expression of CD69 and CD103. Other cytotoxic molecules, including granzymes A, H, K, and M, were variably expressed across tissues, and memory CD8+ T cells targeting persistent viruses expressed multiple granzymes coordinately. In an in vitro tonsil system, transforming growth factor-\u03b2 induced discordant regulation of cytotoxic molecules and CD103. Combined with interleukin-15, this circuitry modulated proliferation and the acquisition of redirected killing activity via perforin and granzyme B. Our findings suggest that human memory CD8+ T cell cytotoxicity is intricately regulated by environmental cues reflecting tissue location and antigen specificity.", "doi": "10.1126/sciimmunol.adq4881", "pmid": "40680144", "labels": {"Affinity Proteomics Stockholm": "Service"}, "xrefs": [], "notes": [], "created": "2025-10-13T13:28:04.756Z", "modified": "2025-11-25T21:31:43.911Z"}]}