{"entity": "researcher", "timestamp": "2026-08-15T06:29:19.763Z", "family": "K\u00f6hler", "given": "Stephan J", "initials": "SJ", "orcid": "0000-0001-9707-9023", "affiliations": ["Department of Aquatic Sciences and Assessment, Soil-Water-Environment Center, Swedish University of Agricultural Sciences, Box 7050, SE-750 07, Uppsala, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/955fd789119a4e5f9ec314275095a70e.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/955fd789119a4e5f9ec314275095a70e"}}, "publications": [{"entity": "publication", "iuid": "872c7a106b1e474792fbaa94e504a7ca", "links": {"self": {"href": "https://publications.scilifelab.se/publication/872c7a106b1e474792fbaa94e504a7ca.json"}, "display": {"href": "https://publications.scilifelab.se/publication/872c7a106b1e474792fbaa94e504a7ca"}}, "title": "Avoiding Mitochondrial Apoptosis by the Bcl-2-Driven Bax Oligomerization on Membrane Surfaces", "authors": [{"family": "Ayscough", "given": "Sophie E", "initials": "SE"}, {"family": "Clifton", "given": "Luke A", "initials": "LA", "orcid": "0000-0001-8754-362X", "researcher": {"href": "https://publications.scilifelab.se/researcher/e26f83508bc7451a9d66123eb9ea747c.json"}}, {"family": "\u00c5d\u00e9n", "given": "J\u00f6rgen", "initials": "J", "orcid": "0000-0002-4480-1219", "researcher": {"href": "https://publications.scilifelab.se/researcher/00206abad0de45eb90dc6bd3d5f3cf22.json"}}, {"family": "K\u00f6hler", "given": "Sebastian", "initials": "S", "orcid": "0000-0001-9707-9023", "researcher": {"href": "https://publications.scilifelab.se/researcher/955fd789119a4e5f9ec314275095a70e.json"}}, {"family": "Paracini", "given": "Nicol\u00f2", "initials": "N", "orcid": "0000-0003-3178-4867", "researcher": {"href": "https://publications.scilifelab.se/researcher/640d007b304247b9bebd4837bdeba788.json"}}, {"family": "Doutch", "given": "James", "initials": "J", "orcid": "0000-0003-0747-8368", "researcher": {"href": "https://publications.scilifelab.se/researcher/4e1b2f6a54554068b76215bdc2554b61.json"}}, {"family": "Bragginton", "given": "\u00c9il\u00eds C", "initials": "\u00c9C"}, {"family": "Leung", "given": "Anna E", "initials": "AE", "orcid": "0000-0002-8196-9774", "researcher": {"href": "https://publications.scilifelab.se/researcher/0f083dbf21ad4cdbbbea7248331949bf.json"}}, {"family": "Bogojevic", "given": "Oliver", "initials": "O"}, {"family": "Poon", "given": "Jia Fei", "initials": "JF", "orcid": "0000-0002-5568-7415", "researcher": {"href": "https://publications.scilifelab.se/researcher/743b924c930841eea6c30fddd62f7a10.json"}}, {"family": "Nagy", "given": "Tam\u00e1s Mil\u00e1n", "initials": "TM", "orcid": "0000-0003-4766-1992", "researcher": {"href": "https://publications.scilifelab.se/researcher/22a9fd6e6a4e46b2ae2bc0166f62e092.json"}}, {"family": "Wacklin-Knecht", "given": "Hanna P", "initials": "HP", "orcid": "0000-0002-5248-1147", "researcher": {"href": "https://publications.scilifelab.se/researcher/962e09a261294bcea30f21ae45b23177.json"}}, {"family": "Gr\u00f6bner", "given": "Gerhard", "initials": "G", "orcid": "0000-0001-7380-8797", "researcher": {"href": "https://publications.scilifelab.se/researcher/85bd86ebc85d4653bc880bc9be25bc80.json"}}], "type": "journal-article", "published": "2026-03-20", "journal": {"title": "ACS Chem. Biol.", "issn": "1554-8929", "volume": "21", "issue": "3", "pages": "565-576", "issn-l": null}, "abstract": "The Bcl-2 family of proteins governs mitochondrial outer membrane (MOM) permeabilization, a critical step in apoptosis that is dysfunctional in many cancers. Although cellular studies have long implicated direct interactions between the pore-forming apoptotic Bax protein and its opponent, the antiapoptotic Bcl-2 protein in apoptosis regulation, the underlying basic principles behind this control remained unresolved. To provide in-depth insight, we carried out a systematic biophysical study in which we utilized neutron reflectometry (NR) and ATR-FTIR to elucidate the molecular communication between those proteins in and around the mitochondrial membrane environment. The spatial and temporal changes across model MOM surfaces were resolved during the interaction of Bax with Bcl-2. The NR-derived membrane surface Bax distributions suggested that Bcl-2 mediated Bax sequestration through both Bcl-2/Bax heterodimerization and Bax/Bax oligomerization. Kinetic analysis revealed a two-step process: rapid formation of Bcl-2/Bax heterodimers, followed by slower Bax oligomerization on these complexes. Importantly, this sequestration mechanism was also observed in the presence of cardiolipin, a lipid known to promote the formation of an apoptotic pore by Bax in the absence of Bcl-2. These findings suggest a fundamental mechanism by which cancer cells may evade apoptosis by exploiting Bcl-2's ability to neutralize Bax through structural entrapment, even if excess Bax is present, either in response to treatment or natural death signals.", "doi": "10.1021/acschembio.5c00913", "pmid": "41705766", "labels": {"Swedish NMR Centre": "Collaborative"}, "xrefs": [], "notes": [], "created": "2026-03-06T09:26:31.753Z", "modified": "2026-03-24T09:09:22.716Z"}, {"entity": "publication", "iuid": "2087c60a5a82438891ed417372e22fe0", "links": {"self": {"href": "https://publications.scilifelab.se/publication/2087c60a5a82438891ed417372e22fe0.json"}, "display": {"href": "https://publications.scilifelab.se/publication/2087c60a5a82438891ed417372e22fe0"}}, "title": "Ectomycorrhizal fungi integrate nitrogen mobilisation and mineral weathering in boreal forest soil.", "authors": [{"family": "Mahmood", "given": "Shahid", "initials": "S", "orcid": "0000-0001-6280-4387", "researcher": {"href": "https://publications.scilifelab.se/researcher/af95eec78fe44938b73a8d1e91484b66.json"}}, {"family": "Fahad", "given": "Zaenab", "initials": "Z", "orcid": "0009-0000-5835-5160", "researcher": {"href": "https://publications.scilifelab.se/researcher/4c20dfdb0c7340778f20b1e239808c56.json"}}, {"family": "Bolou-Bi", "given": "Emile B", "initials": "EB", "orcid": "0000-0001-7803-3214", "researcher": {"href": "https://publications.scilifelab.se/researcher/488aee2af3a844d1b7fbbfcfde399d66.json"}}, {"family": "King", "given": "Katharine", "initials": "K", "orcid": "0009-0009-8075-9466", "researcher": {"href": "https://publications.scilifelab.se/researcher/2421e6cb187745cc909f72a6ac9375ee.json"}}, {"family": "K\u00f6hler", "given": "Stephan J", "initials": "SJ", "orcid": "0000-0001-9707-9023", "researcher": {"href": "https://publications.scilifelab.se/researcher/955fd789119a4e5f9ec314275095a70e.json"}}, {"family": "Bishop", "given": "Kevin", "initials": "K", "orcid": "0000-0002-8057-1051", "researcher": {"href": "https://publications.scilifelab.se/researcher/2cf39f8ead0744f591dcdbcb6caee99a.json"}}, {"family": "Ekblad", "given": "Alf", "initials": "A", "orcid": "0000-0003-4384-5014", "researcher": {"href": "https://publications.scilifelab.se/researcher/55b407368cf342698c0b681ef8cf2ba9.json"}}, {"family": "Finlay", "given": "Roger D", "initials": "RD", "orcid": "0000-0002-3652-2930", "researcher": {"href": "https://publications.scilifelab.se/researcher/9cf62270530f402faa530c435cb6829c.json"}}], "type": "journal article", "published": "2023-09-11", "journal": {"title": "New Phytol.", "issn": "1469-8137", "issn-l": "0028-646X"}, "abstract": "Tree growth in boreal forests is driven by ectomycorrhizal fungal mobilisation of organic nitrogen and mineral nutrients in soils with discrete organic and mineral horizons. However, there are no studies of how ectomycorrhizal mineral weathering and organic nitrogen mobilisation processes are integrated across the soil profile. We studied effects of organic matter (OM) availability on ectomycorrhizal functioning by altering the proportions of natural organic and mineral soil in reconstructed podzol profiles containing Pinus sylvestris plants, using 13 CO2 pulse labelling, patterns of naturally occurring stable isotopes (26 Mg and 15 N) and high-throughput DNA sequencing of fungal amplicons. Reduction in OM resulted in nitrogen limitation of plant growth and decreased allocation of photosynthetically derived carbon and mycelial growth in mineral horizons. Fractionation patterns of 26 Mg indicated that magnesium mobilisation and uptake occurred primarily in the deeper mineral horizon and was driven by carbon allocation to ectomycorrhizal mycelium. In this horizon, relative abundance of ectomycorrhizal fungi, carbon allocation and base cation mobilisation all increased with increased OM availability. Allocation of carbon through ectomycorrhizal fungi integrates organic nitrogen mobilisation and mineral weathering across soil horizons, improving the efficiency of plant nutrient acquisition. Our findings have fundamental implications for sustainable forest management and belowground carbon sequestration.", "doi": "10.1111/nph.19260", "pmid": "37697631", "labels": {"NGI Long read": "Service", "National Genomics Infrastructure": "Service", "NGI Uppsala (Uppsala Genome Center)": "Service", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [], "notes": [], "created": "2023-10-10T08:50:30.284Z", "modified": "2024-01-16T13:48:32.278Z"}]}