{"entity": "researcher", "timestamp": "2026-08-07T18:18:41.302Z", "family": "Oliver", "given": "Cecilia", "initials": "C", "orcid": "0000-0002-5231-7910", "affiliations": ["Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural, Sciences and Linnean Center for Plant Biology, Uppsala, Sweden. cecilia.oliver.velasco@slu.se."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/8fdb426c5e2e48e985eb8939a1c40d6e.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/8fdb426c5e2e48e985eb8939a1c40d6e"}}, "publications": [{"entity": "publication", "iuid": "90262d7622894dd583b0207a75b665a6", "links": {"self": {"href": "https://publications.scilifelab.se/publication/90262d7622894dd583b0207a75b665a6.json"}, "display": {"href": "https://publications.scilifelab.se/publication/90262d7622894dd583b0207a75b665a6"}}, "title": "Accumulation dynamics of ARGONAUTE proteins during meiosis in Arabidopsis.", "authors": [{"family": "Oliver", "given": "Cecilia", "initials": "C", "orcid": "0000-0002-5231-7910", "researcher": {"href": "https://publications.scilifelab.se/researcher/8fdb426c5e2e48e985eb8939a1c40d6e.json"}}, {"family": "Martinez", "given": "German", "initials": "G", "orcid": "0000-0002-5215-0866", "researcher": {"href": "https://publications.scilifelab.se/researcher/591f629ea8ed44c2bd9cd417dcebd8bc.json"}}], "type": "journal article", "published": "2022-06-00", "journal": {"title": "Plant Reprod", "issn": "2194-7961", "volume": "35", "issue": "2", "pages": "153-160", "issn-l": "2194-7953"}, "abstract": "Meiosis is a specialized cell division that is key for reproduction and genetic diversity in sexually reproducing plants. Recently, different RNA silencing pathways have been proposed to carry a specific activity during meiosis, but the pathways involved during this process remain unclear. Here, we explored the subcellular localization of different ARGONAUTE (AGO) proteins, the main effectors of RNA silencing, during male meiosis in Arabidopsis thaliana using immunolocalizations with commercially available antibodies. We detected the presence of AGO proteins associated with posttranscriptional gene silencing (AGO1, 2, and 5) in the cytoplasm and the nucleus, while AGOs associated with transcriptional gene silencing (AGO4 and 9) localized exclusively in the nucleus. These results indicate that the localization of different AGOs correlates with their predicted roles at the transcriptional and posttranscriptional levels and provide an overview of their timing and potential role during meiosis.", "doi": "10.1007/s00497-021-00434-z", "pmid": "34812935", "labels": {"Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pii", "key": "10.1007/s00497-021-00434-z"}, {"db": "pmc", "key": "PMC9110482"}], "notes": [], "created": "2022-11-09T15:46:54.059Z", "modified": "2024-01-16T13:48:36.366Z"}, {"entity": "publication", "iuid": "70304e2e74c14f8c98b28d736df71cf4", "links": {"self": {"href": "https://publications.scilifelab.se/publication/70304e2e74c14f8c98b28d736df71cf4.json"}, "display": {"href": "https://publications.scilifelab.se/publication/70304e2e74c14f8c98b28d736df71cf4"}}, "title": "The miRNome function transitions from regulating developmental genes to transposable elements during pollen maturation.", "authors": [{"family": "Oliver", "given": "Cecilia", "initials": "C", "orcid": "0000-0002-5231-7910", "researcher": {"href": "https://publications.scilifelab.se/researcher/8fdb426c5e2e48e985eb8939a1c40d6e.json"}}, {"family": "Annacondia", "given": "Maria Luz", "initials": "ML", "orcid": "0000-0001-7998-8362", "researcher": {"href": "https://publications.scilifelab.se/researcher/7b4d89a422254e47a38fe438fe99bdaf.json"}}, {"family": "Wang", "given": "Zhenxing", "initials": "Z", "orcid": "0000-0001-5102-7121", "researcher": {"href": "https://publications.scilifelab.se/researcher/4b6c9f88c65d4fbc94da6d7598102335.json"}}, {"family": "Jullien", "given": "Pauline E", "initials": "PE", "orcid": "0000-0003-1212-3246", "researcher": {"href": "https://publications.scilifelab.se/researcher/7a97f95d2f1046b3a7027dedf5a31082.json"}}, {"family": "Slotkin", "given": "R Keith", "initials": "RK", "orcid": "0000-0001-9582-3533", "researcher": {"href": "https://publications.scilifelab.se/researcher/ac639e0390ef4140a566e5cbbf65084a.json"}}, {"family": "K\u00f6hler", "given": "Claudia", "initials": "C", "orcid": "0000-0002-2619-4857", "researcher": {"href": "https://publications.scilifelab.se/researcher/accd3f9307614c8ab67154dd5e50cdac.json"}}, {"family": "Martinez", "given": "German", "initials": "G", "orcid": "0000-0002-5215-0866", "researcher": {"href": "https://publications.scilifelab.se/researcher/591f629ea8ed44c2bd9cd417dcebd8bc.json"}}], "type": "journal article", "published": "2022-02-03", "journal": {"title": "Plant Cell", "issn": "1532-298X", "volume": "34", "issue": "2", "pages": "784-801", "issn-l": "1040-4651"}, "abstract": "Animal and plant microRNAs (miRNAs) are essential for the spatio-temporal regulation of development. Together with this role, plant miRNAs have been proposed to target transposable elements (TEs) and stimulate the production of epigenetically active small interfering RNAs. This activity is evident in the plant male gamete containing structure, the male gametophyte or pollen grain. How the dual role of plant miRNAs, regulating both genes and TEs, is integrated during pollen development and which mRNAs are regulated by miRNAs in this cell type at a genome-wide scale are unknown. Here, we provide a detailed analysis of miRNA dynamics and activity during pollen development in Arabidopsis thaliana using small RNA and degradome parallel analysis of RNA end high-throughput sequencing. Furthermore, we uncover miRNAs loaded into the two main active Argonaute (AGO) proteins in the uninuclear and mature pollen grain, AGO1 and AGO5. Our results indicate that the developmental progression from microspore to mature pollen grain is characterized by a transition from miRNAs targeting developmental genes to miRNAs regulating TE activity.", "doi": "10.1093/plcell/koab280", "pmid": "34755870", "labels": {"NGI Short read": "Service", "NGI Uppsala (SNP&SEQ Technology Platform)": "Service", "National Genomics Infrastructure": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC8824631"}, {"db": "pii", "key": "6424912"}], "notes": [], "created": "2022-11-29T09:51:29.116Z", "modified": "2022-11-29T09:51:29.270Z"}]}