{"entity": "researcher", "timestamp": "2026-04-20T22:47:32.825Z", "family": "Arnberg", "given": "N", "initials": "N", "orcid": "0000-0002-7069-6678", "affiliations": ["Department of Clinical Microbiology, Section of Virology, Ume\u00e5 University, Ume\u00e5, Sweden. niklas.arnberg@umu.se lars-anders.carlson@umu.se."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/8764d7981e174798ab8411d36e7d0f1b.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/8764d7981e174798ab8411d36e7d0f1b"}}, "publications": [{"entity": "publication", "iuid": "fae7836eebed4c84bdc531fa2a25e0e9", "links": {"self": {"href": "https://publications.scilifelab.se/publication/fae7836eebed4c84bdc531fa2a25e0e9.json"}, "display": {"href": "https://publications.scilifelab.se/publication/fae7836eebed4c84bdc531fa2a25e0e9"}}, "title": "Extracellular Galectin 4 Drives Immune Evasion and Promotes T-cell Apoptosis in Pancreatic Cancer.", "authors": [{"family": "Lidstr\u00f6m", "given": "Tommy", "initials": "T", "orcid": "0000-0002-1353-316X", "researcher": {"href": "https://publications.scilifelab.se/researcher/1011864c212648d6b9132d33928c7752.json"}}, {"family": "Cumming", "given": "Joshua", "initials": "J", "orcid": "0000-0003-3661-7443", "researcher": {"href": "https://publications.scilifelab.se/researcher/fd0f3e688ff047ef8e814134c0446774.json"}}, {"family": "Gaur", "given": "Rahul", "initials": "R", "orcid": "0000-0003-0529-7176", "researcher": {"href": "https://publications.scilifelab.se/researcher/9990aa5c88384a5e8a012fb8b009b1de.json"}}, {"family": "Fr\u00e4ngsmyr", "given": "Lars", "initials": "L", "orcid": "0000-0002-6523-5156", "researcher": {"href": "https://publications.scilifelab.se/researcher/4d4ef137f0024aa5ae5954247c10eeb3.json"}}, {"family": "Pateras", "given": "Ioannis S", "initials": "IS", "orcid": "0000-0002-6406-9811", "researcher": {"href": "https://publications.scilifelab.se/researcher/c4a203631ae04776b0583407cc1733ca.json"}}, {"family": "Mickert", "given": "Matthias J", "initials": "MJ", "orcid": "0000-0002-1542-5998", "researcher": {"href": "https://publications.scilifelab.se/researcher/4e269d0e706647829da55f108f69f95b.json"}}, {"family": "Franklin", "given": "Oskar", "initials": "O", "orcid": "0000-0002-3777-6887", "researcher": {"href": "https://publications.scilifelab.se/researcher/3ef2966672d941f881f588707c1edd37.json"}}, {"family": "Forsell", "given": "Mattias N E", "initials": "MNE", "orcid": "0000-0001-6904-742X", "researcher": {"href": "https://publications.scilifelab.se/researcher/5947732f625a43e8a08055fc214649b6.json"}}, {"family": "Arnberg", "given": "Niklas", "initials": "N", "orcid": "0000-0002-7069-6678", "researcher": {"href": "https://publications.scilifelab.se/researcher/8764d7981e174798ab8411d36e7d0f1b.json"}}, {"family": "Dongre", "given": "Mitesh", "initials": "M", "orcid": "0000-0002-7151-1137", "researcher": {"href": "https://publications.scilifelab.se/researcher/6acd00be909d41399277be66d9600d41.json"}}, {"family": "Patthey", "given": "Cedric", "initials": "C", "orcid": "0000-0002-2627-9578", "researcher": {"href": "https://publications.scilifelab.se/researcher/6b7707e8bd3d4e029fb1e1f43df86ad4.json"}}, {"family": "\u00d6hlund", "given": "Daniel", "initials": "D", "orcid": "0000-0002-5847-2778", "researcher": {"href": "https://publications.scilifelab.se/researcher/42e9e473f68c460098a37e22d0a41369.json"}}], "type": "journal article", "published": "2023-01-03", "journal": {"title": "Cancer Immunol Res", "issn": "2326-6074", "volume": "11", "issue": "1", "pages": "72-92", "issn-l": null}, "abstract": "Pancreatic ductal adenocarcinoma (PDAC) is characterized by rich deposits of extracellular matrix (ECM), affecting the pathophysiology of the disease. Here, we identified galectin 4 (gal 4) as a cancer cell-produced protein that was deposited into the ECM of PDAC tumors and detected high-circulating levels of gal 4 in patients with PDAC. In orthotopic transplantation experiments, we observed increased infiltration of T cells and prolonged survival in immunocompetent mice transplanted with cancer cells with reduced expression of gal 4. Increased survival was not observed in immunodeficient RAG1-/- mice, demonstrating that the effect was mediated by the adaptive immune system. By performing single-cell RNA-sequencing, we found that the myeloid compartment and cancer-associated fibroblast (CAF) subtypes were altered in the transplanted tumors. Reduced gal 4 expression associated with a higher proportion of myofibroblastic CAFs and reduced numbers of inflammatory CAFs. We also found higher proportions of M1 macrophages, T cells, and antigen-presenting dendritic cells in tumors with reduced gal 4 expression. Using a coculture system, we observed that extracellular gal 4 induced apoptosis in T cells by binding N-glycosylation residues on CD3\u03b5/\u03b4. Hence, we show that gal 4 is involved in immune evasion and identify gal 4 as a promising drug target for overcoming immunosuppression in PDAC.", "doi": "10.1158/2326-6066.CIR-21-1088", "pmid": "36478037", "labels": {"Clinical Genomics Ume\u00e5": "Service", "Bioinformatics Support for Computational Resources": "Service", "Clinical Genomics": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC9808371"}, {"db": "pii", "key": "711503"}], "notes": [], "created": "2023-11-29T09:42:40.822Z", "modified": "2024-11-25T10:29:05.262Z"}, {"entity": "publication", "iuid": "e37b1471124a457e810b1b48da79d5c4", "links": {"self": {"href": "https://publications.scilifelab.se/publication/e37b1471124a457e810b1b48da79d5c4.json"}, "display": {"href": "https://publications.scilifelab.se/publication/e37b1471124a457e810b1b48da79d5c4"}}, "title": "Human species D adenovirus hexon capsid protein mediates cell entry through a direct interaction with CD46.", "authors": [{"family": "Persson", "given": "B David", "initials": "BD"}, {"family": "John", "given": "Lijo", "initials": "L", "orcid": "0000-0002-5459-5359", "researcher": {"href": "https://publications.scilifelab.se/researcher/3c8a9610a72c421e9a347c4d951985b9.json"}}, {"family": "Rafie", "given": "Karim", "initials": "K", "orcid": "0000-0003-2418-0061", "researcher": {"href": "https://publications.scilifelab.se/researcher/0c32b813de00417e8fa716ea5c6059c7.json"}}, {"family": "Strebl", "given": "Michael", "initials": "M", "orcid": "0000-0001-9287-5549", "researcher": {"href": "https://publications.scilifelab.se/researcher/9b9ca05cf53d4a44bd103d09c1ebd3b4.json"}}, {"family": "Fr\u00e4ngsmyr", "given": "Lars", "initials": "L"}, {"family": "Ballmann", "given": "Monika Z", "initials": "MZ"}, {"family": "Mindler", "given": "Katja", "initials": "K", "orcid": "0000-0002-6730-4151", "researcher": {"href": "https://publications.scilifelab.se/researcher/7e33242648164606ac361780ab34ba0f.json"}}, {"family": "Havenga", "given": "Menzo", "initials": "M", "orcid": "0000-0003-4094-6362", "researcher": {"href": "https://publications.scilifelab.se/researcher/2e3ae342284f4a049e22b9a5c4f5914b.json"}}, {"family": "Lemckert", "given": "Angelique", "initials": "A", "orcid": "0000-0003-0151-0953", "researcher": {"href": "https://publications.scilifelab.se/researcher/cfedb8d7b16142ff91a2c235d86f0020.json"}}, {"family": "Stehle", "given": "Thilo", "initials": "T"}, {"family": "Carlson", "given": "Lars-Anders", "initials": "LA", "orcid": "0000-0003-2342-6488", "researcher": {"href": "https://publications.scilifelab.se/researcher/ba0e366ce21e49b48212bbed5a0a7bd1.json"}}, {"family": "Arnberg", "given": "Niklas", "initials": "N", "orcid": "0000-0002-7069-6678", "researcher": {"href": "https://publications.scilifelab.se/researcher/8764d7981e174798ab8411d36e7d0f1b.json"}}], "type": "journal article", "published": "2021-01-19", "journal": {"title": "Proc. Natl. Acad. Sci. U.S.A.", "issn": "1091-6490", "volume": "118", "issue": "3", "issn-l": "0027-8424"}, "abstract": "Human adenovirus species D (HAdV-D) types are currently being explored as vaccine vectors for coronavirus disease 2019 (COVID-19) and other severe infectious diseases. The efficacy of such vector-based vaccines depends on functional interactions with receptors on host cells. Adenoviruses of different species are assumed to enter host cells mainly by interactions between the knob domain of the protruding fiber capsid protein and cellular receptors. Using a cell-based receptor-screening assay, we identified CD46 as a receptor for HAdV-D56. The function of CD46 was validated in infection experiments using cells lacking and overexpressing CD46, and by competition infection experiments using soluble CD46. Remarkably, unlike HAdV-B types that engage CD46 through interactions with the knob domain of the fiber protein, HAdV-D types infect host cells through a direct interaction between CD46 and the hexon protein. Soluble hexon proteins (but not fiber knob) inhibited HAdV-D56 infection, and surface plasmon analyses demonstrated that CD46 binds to HAdV-D hexon (but not fiber knob) proteins. Cryoelectron microscopy analysis of the HAdV-D56 virion-CD46 complex confirmed the interaction and showed that CD46 binds to the central cavity of hexon trimers. Finally, soluble CD46 inhibited infection by 16 out of 17 investigated HAdV-D types, suggesting that CD46 is an important receptor for a large group of adenoviruses. In conclusion, this study identifies a noncanonical entry mechanism used by human adenoviruses, which adds to the knowledge of adenovirus biology and can also be useful for development of adenovirus-based vaccine vectors.", "doi": "10.1073/pnas.2020732118", "pmid": "33384338", "labels": {"Cryo-EM": "Service"}, "xrefs": [{"db": "pii", "key": "2020732118"}, {"db": "pmc", "key": "PMC7826407"}], "notes": [], "created": "2021-12-13T22:04:11.352Z", "modified": "2021-12-13T22:04:11.571Z"}, {"entity": "publication", "iuid": "f2cdfa285b444ec9b4e48be0907149d9", "links": {"self": {"href": "https://publications.scilifelab.se/publication/f2cdfa285b444ec9b4e48be0907149d9.json"}, "display": {"href": "https://publications.scilifelab.se/publication/f2cdfa285b444ec9b4e48be0907149d9"}}, "title": "The structure of enteric human adenovirus 41-A leading cause of diarrhea in children.", "authors": [{"family": "Rafie", "given": "K", "initials": "K", "orcid": "0000-0003-2418-0061", "researcher": {"href": "https://publications.scilifelab.se/researcher/0c32b813de00417e8fa716ea5c6059c7.json"}}, {"family": "Lenman", "given": "A", "initials": "A", "orcid": "0000-0001-5109-9408", "researcher": {"href": "https://publications.scilifelab.se/researcher/c41b8142e00444afa728b6e4a2ae331a.json"}}, {"family": "Fuchs", "given": "J", "initials": "J"}, {"family": "Rajan", "given": "A", "initials": "A", "orcid": "0000-0002-4873-8528", "researcher": {"href": "https://publications.scilifelab.se/researcher/261182551e72486895b04cebcaa22d69.json"}}, {"family": "Arnberg", "given": "N", "initials": "N", "orcid": "0000-0002-7069-6678", "researcher": {"href": "https://publications.scilifelab.se/researcher/8764d7981e174798ab8411d36e7d0f1b.json"}}, {"family": "Carlson", "given": "L-A", "initials": "LA", "orcid": "0000-0003-2342-6488", "researcher": {"href": "https://publications.scilifelab.se/researcher/ba0e366ce21e49b48212bbed5a0a7bd1.json"}}], "type": "journal article", "published": "2021-01-00", "journal": {"title": "Sci Adv", "issn": "2375-2548", "volume": "7", "issue": "2", "issn-l": "2375-2548"}, "abstract": "Human adenovirus (HAdV) types F40 and F41 are a prominent cause of diarrhea and diarrhea-associated mortality in young children worldwide. These enteric HAdVs differ notably in tissue tropism and pathogenicity from respiratory and ocular adenoviruses, but the structural basis for this divergence has been unknown. Here, we present the first structure of an enteric HAdV-HAdV-F41-determined by cryo-electron microscopy to a resolution of 3.8 \u00c5. The structure reveals extensive alterations to the virion exterior as compared to nonenteric HAdVs, including a unique arrangement of capsid protein IX. The structure also provides new insights into conserved aspects of HAdV architecture such as a proposed location of core protein V, which links the viral DNA to the capsid, and assembly-induced conformational changes in the penton base protein. Our findings provide the structural basis for adaptation of enteric HAdVs to a fundamentally different tissue tropism.", "doi": "10.1126/sciadv.abe0974", "pmid": "33523995", "labels": {"Cryo-EM": "Service", "Glycoproteomics and MS Proteomics": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC7793593"}, {"db": "pii", "key": "7/2/eabe0974"}], "notes": [], "created": "2022-04-01T06:47:38.187Z", "modified": "2024-01-16T13:46:30.920Z"}, {"entity": "publication", "iuid": "736d53a3e91243fe9fea1220d17bc7a1", "links": {"self": {"href": "https://publications.scilifelab.se/publication/736d53a3e91243fe9fea1220d17bc7a1.json"}, "display": {"href": "https://publications.scilifelab.se/publication/736d53a3e91243fe9fea1220d17bc7a1"}}, "title": "Enteric Species F Human Adenoviruses use Laminin-Binding Integrins as Co-Receptors for Infection of Ht-29 Cells.", "authors": [{"family": "Rajan", "given": "Anandi", "initials": "A"}, {"family": "Persson", "given": "B David", "initials": "BD"}, {"family": "Fr\u00e4ngsmyr", "given": "Lars", "initials": "L"}, {"family": "Olofsson", "given": "Annelie", "initials": "A"}, {"family": "Sandblad", "given": "Linda", "initials": "L", "orcid": "0000-0003-3492-3287", "researcher": {"href": "https://publications.scilifelab.se/researcher/070825e0190a4e9a932e79663d2bc89f.json"}}, {"family": "Heino", "given": "Jyrki", "initials": "J"}, {"family": "Takada", "given": "Yoshikazu", "initials": "Y"}, {"family": "Mould", "given": "A Paul", "initials": "AP"}, {"family": "Schnapp", "given": "Lynn M", "initials": "LM"}, {"family": "Gall", "given": "Jason", "initials": "J"}, {"family": "Arnberg", "given": "Niklas", "initials": "N", "orcid": "0000-0002-7069-6678", "researcher": {"href": "https://publications.scilifelab.se/researcher/8764d7981e174798ab8411d36e7d0f1b.json"}}], "type": "journal article", "published": "2018-07-03", "journal": {"title": "Sci Rep", "issn": "2045-2322", "volume": "8", "issue": "1", "pages": "10019", "issn-l": "2045-2322"}, "abstract": "The enteric species F human adenovirus types 40 and 41 (HAdV-40 and -41) are the third most common cause of infantile gastroenteritis in the world. Knowledge about HAdV-40 and -41 cellular infection is assumed to be fundamentally different from that of other HAdVs since HAdV-40 and -41 penton bases lack the \u03b1V-integrin-interacting RGD motif. This motif is used by other HAdVs mainly for internalization and endosomal escape. We hypothesised that the penton bases of HAdV-40 and -41 interact with integrins independently of the RGD motif. HAdV-41 transduction of a library of rodent cells expressing specific human integrin subunits pointed to the use of laminin-binding \u03b12-, \u03b13- and \u03b16-containing integrins as well as other integrins as candidate co-receptors. Specific laminins prevented internalisation and infection, and recombinant, soluble HAdV-41 penton base proteins prevented infection of human intestinal HT-29 cells. Surface plasmon resonance analysis demonstrated that HAdV-40 and -41 penton base proteins bind to \u03b16-containing integrins with an affinity similar to that of previously characterised penton base:integrin interactions. With these results, we propose that laminin-binding integrins are co-receptors for HAdV-40 and -41.", "doi": "10.1038/s41598-018-28255-7", "pmid": "29968781", "labels": {"Cryo-EM": "Service"}, "xrefs": [{"db": "pii", "key": "10.1038/s41598-018-28255-7"}, {"db": "pmc", "key": "PMC6030200"}], "notes": [], "created": "2019-01-10T21:29:28.960Z", "modified": "2021-07-05T17:20:56.109Z"}]}