{"entity": "researcher", "timestamp": "2026-07-13T09:38:24.783Z", "family": "Suarez", "given": "Carolina", "initials": "C", "orcid": "0000-0001-5988-4048", "affiliations": [], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/86cadb16f7cf45eca8030af1a8ae860e.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/86cadb16f7cf45eca8030af1a8ae860e"}}, "publications": [{"entity": "publication", "iuid": "9cffa610a5254003956c5dedb591e1d0", "links": {"self": {"href": "https://publications.scilifelab.se/publication/9cffa610a5254003956c5dedb591e1d0.json"}, "display": {"href": "https://publications.scilifelab.se/publication/9cffa610a5254003956c5dedb591e1d0"}}, "title": "Microbial communities in slow sand filters for drinking water treatment adapt to organic matter altered by ozonation.", "authors": [{"family": "Rosenqvist", "given": "Tage", "initials": "T"}, {"family": "Hilding", "given": "Johanna", "initials": "J"}, {"family": "Suarez", "given": "Carolina", "initials": "C", "orcid": "0000-0001-5988-4048", "researcher": {"href": "https://publications.scilifelab.se/researcher/86cadb16f7cf45eca8030af1a8ae860e.json"}}, {"family": "Paul", "given": "Catherine J", "initials": "CJ", "orcid": "0000-0003-0323-8359", "researcher": {"href": "https://publications.scilifelab.se/researcher/0c169e96b18c403b9c18228126e6fd8d.json"}}], "type": "journal article", "published": "2025-02-15", "journal": {"title": "Water Res.", "issn": "1879-2448", "issn-l": "0043-1354", "volume": "270", "issue": null, "pages": "122843"}, "abstract": "Changing natural organic matter quality from anthropogenic activity and stricter requirements for micropollutant removal challenges existing systems for drinking water production. Ozonation of water followed by biofiltration, such as passage through a slow sand filter (SSF), is a partial solution. Biofiltration relies on biofilms (microbial communities within extracellular matrices). However, the effects of ozonation on SSF microbial communities are unknown. In this study, genome-resolved and read-based metagenomics were used to compare the microbial communities of two full-scale SSFs employing conventional pre-treatment to a 20 m2 SSF operated in parallel with ozonation as additional pre-treatment. The SSF microbial community receiving ozonated water was less diverse than those receiving non-ozonated water. Families Hyphomicrobiaceae, Acetobacteraceae, Sphingomonadaceae and Burkholderiaceae were more abundant when ozone was used, as were genes for metabolism of single-carbon organic compounds. Conversely, genes for metabolism of aromatic compounds and fatty acids were less abundant. Metagenome assembled genomes associated with the non-ozonated SSFs were enriched with several glycoside hydrolases, while those associated with the ozonated SSF were enriched with genes for 1-2 carbon compound metabolism. No indications of increased microbial risk (pathogens or antibiotic resistance genes) were detected as a consequence of ozonation. This study shows how microbial communities of SSFs adapt to changes in organic matter quality, highlighting the key role of biofilters for production of safe and sustainable drinking water in a changing climate.", "doi": "10.1016/j.watres.2024.122843", "pmid": "39612821", "labels": {"Bioinformatics Support for Computational Resources": "Service", "NGI Short read": "Service", "National Genomics Infrastructure": "Service", "NGI Stockholm (Genomics Production)": "Service"}, "xrefs": [{"db": "pii", "key": "S0043-1354(24)01742-1"}], "notes": [], "created": "2025-02-28T14:13:29.572Z", "modified": "2025-05-27T08:38:18.414Z"}, {"entity": "publication", "iuid": "bb49b19b908c48f6b2159e5e8e208b77", "links": {"self": {"href": "https://publications.scilifelab.se/publication/bb49b19b908c48f6b2159e5e8e208b77.json"}, "display": {"href": "https://publications.scilifelab.se/publication/bb49b19b908c48f6b2159e5e8e208b77"}}, "title": "Novel and unusual genes for nitrogen and metal cycling in Planctomycetota- and KSB1-affiliated metagenome-assembled genomes reconstructed from a marine subsea tunnel.", "authors": [{"family": "Suarez", "given": "Carolina", "initials": "C", "orcid": "0000-0001-5988-4048", "researcher": {"href": "https://publications.scilifelab.se/researcher/86cadb16f7cf45eca8030af1a8ae860e.json"}}, {"family": "Hackl", "given": "Thomas", "initials": "T", "orcid": "0000-0002-0022-320X", "researcher": {"href": "https://publications.scilifelab.se/researcher/0d7de95e834842c3b506c92f9ef648f5.json"}}, {"family": "Wilen", "given": "Britt-Marie", "initials": "BM", "orcid": "0000-0001-6155-7759", "researcher": {"href": "https://publications.scilifelab.se/researcher/17fcd853ec9d43bda259e8d440a92eb7.json"}}, {"family": "Persson", "given": "Frank", "initials": "F", "orcid": "0000-0002-0269-9375", "researcher": {"href": "https://publications.scilifelab.se/researcher/40290cae1a7e44c1939801323653b31c.json"}}, {"family": "Hagelia", "given": "Per", "initials": "P"}, {"family": "Jetten", "given": "Mike S M", "initials": "MSM", "orcid": "0000-0002-4691-7039", "researcher": {"href": "https://publications.scilifelab.se/researcher/1b43ecab3b254c8eb61a10099e3fda63.json"}}, {"family": "Dalcin Martins", "given": "Paula", "initials": "P", "orcid": "0000-0003-1242-0267", "researcher": {"href": "https://publications.scilifelab.se/researcher/fd51c24d64564b0ca5d066c5dcabbecd.json"}}], "type": "journal article", "published": "2023-01-17", "journal": {"title": "FEMS Microbiol. Lett.", "issn": "1574-6968", "volume": "370", "issn-l": "0378-1097"}, "abstract": "The Oslofjord subsea road tunnel is a unique environment in which the typically anoxic marine deep subsurface is exposed to oxygen. Concrete biodeterioration and steel corrosion in the tunnel have been linked to the growth of iron- and manganese-oxidizing biofilms in areas of saline water seepage. Surprisingly, previous 16S rRNA gene surveys of biofilm samples revealed microbial communities dominated by sequences affiliated with nitrogen-cycling microorganisms. This study aimed to identify microbial genomes with metabolic potential for novel nitrogen- and metal-cycling reactions, representing biofilm microorganisms that could link these cycles and play a role in concrete biodeterioration. We reconstructed 33 abundant, novel metagenome-assembled genomes (MAGs) affiliated with the phylum Planctomycetota and the candidate phylum KSB1. We identified novel and unusual genes and gene clusters in these MAGs related to anaerobic ammonium oxidation, nitrite oxidation, and other nitrogen-cycling reactions. Additionally, 26 of 33 MAGs also had the potential for iron, manganese, and arsenite cycling, suggesting that bacteria represented by these genomes might couple these reactions. Our results expand the diversity of microorganisms putatively involved in nitrogen and metal cycling, and contribute to our understanding of potential biofilm impacts on built infrastructure.", "doi": "10.1093/femsle/fnad049", "pmid": "37291701", "labels": {"Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pii", "key": "7192431"}], "notes": [], "created": "2023-11-27T21:52:12.844Z", "modified": "2024-01-16T13:48:34.124Z"}, {"entity": "publication", "iuid": "fc535be61b6b43f8869dd1955c2d0546", "links": {"self": {"href": "https://publications.scilifelab.se/publication/fc535be61b6b43f8869dd1955c2d0546.json"}, "display": {"href": "https://publications.scilifelab.se/publication/fc535be61b6b43f8869dd1955c2d0546"}}, "title": "Disturbance-based management of ecosystem services and disservices in partial nitritation-anammox biofilms.", "authors": [{"family": "Suarez", "given": "Carolina", "initials": "C", "orcid": "0000-0001-5988-4048", "researcher": {"href": "https://publications.scilifelab.se/researcher/86cadb16f7cf45eca8030af1a8ae860e.json"}}, {"family": "Sedlacek", "given": "Christopher J", "initials": "CJ"}, {"family": "Gustavsson", "given": "David J I", "initials": "DJI"}, {"family": "Eiler", "given": "Alexander", "initials": "A"}, {"family": "Modin", "given": "Oskar", "initials": "O", "orcid": "0000-0002-9232-6096", "researcher": {"href": "https://publications.scilifelab.se/researcher/e1cfa2dd97cc472c9307498a65b5a8c6.json"}}, {"family": "Hermansson", "given": "Malte", "initials": "M"}, {"family": "Persson", "given": "Frank", "initials": "F", "orcid": "0000-0002-0269-9375", "researcher": {"href": "https://publications.scilifelab.se/researcher/40290cae1a7e44c1939801323653b31c.json"}}], "type": "journal article", "published": "2022-06-08", "journal": {"title": "NPJ Biofilms Microbiomes", "issn": "2055-5008", "issn-l": "2055-5008", "volume": "8", "issue": "1", "pages": "47"}, "abstract": "The resistance and resilience provided by functional redundancy, a common feature of microbial communities, is not always advantageous. An example is nitrite oxidation in partial nitritation-anammox (PNA) reactors designed for nitrogen removal in wastewater treatment, where suppression of nitrite oxidizers like Nitrospira is sought. In these ecosystems, biofilms provide microhabitats with oxygen gradients, allowing the coexistence of aerobic and anaerobic bacteria. We designed a disturbance experiment where PNA biofilms, treating water from a high-rate activated sludge process, were constantly or intermittently exposed to anaerobic sidestream wastewater, which has been proposed to inhibit nitrite oxidizers. With increasing sidestream exposure we observed decreased abundance, alpha-diversity, functional versatility, and hence functional redundancy, among Nitrospira in the PNA biofilms, while the opposite patterns were observed for anammox bacteria within Brocadia. At the same time, species turnover was observed for aerobic ammonia-oxidizing Nitrosomonas populations. The different exposure regimens were associated with metagenomic assembled genomes of Nitrosomonas, Nitrospira, and Brocadia, encoding genes related to N-cycling, substrate usage, and osmotic stress response, possibly explaining the three different patterns by niche differentiation. These findings imply that disturbances can be used to manage the functional redundancy of biofilm microbiomes in a desirable direction, which should be considered when designing operational strategies for wastewater treatment.", "doi": "10.1038/s41522-022-00308-w", "pmid": "35676296", "labels": {"National Genomics Infrastructure": "Service", "NGI Stockholm (Genomics Production)": "Service", "NGI Short read": "Service", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC9178042"}, {"db": "pii", "key": "10.1038/s41522-022-00308-w"}], "notes": [], "created": "2022-08-19T08:38:09.681Z", "modified": "2024-01-16T13:48:36.210Z"}, {"entity": "publication", "iuid": "a014663cca0947d8baf49994504a6cd9", "links": {"self": {"href": "https://publications.scilifelab.se/publication/a014663cca0947d8baf49994504a6cd9.json"}, "display": {"href": "https://publications.scilifelab.se/publication/a014663cca0947d8baf49994504a6cd9"}}, "title": "Thickness determines microbial community structure and function in nitrifying biofilms via deterministic assembly.", "authors": [{"family": "Suarez", "given": "Carolina", "initials": "C", "orcid": "0000-0001-5988-4048", "researcher": {"href": "https://publications.scilifelab.se/researcher/86cadb16f7cf45eca8030af1a8ae860e.json"}}, {"family": "Piculell", "given": "Maria", "initials": "M"}, {"family": "Modin", "given": "Oskar", "initials": "O", "orcid": "0000-0002-9232-6096", "researcher": {"href": "https://publications.scilifelab.se/researcher/e1cfa2dd97cc472c9307498a65b5a8c6.json"}}, {"family": "Langenheder", "given": "Silke", "initials": "S"}, {"family": "Persson", "given": "Frank", "initials": "F"}, {"family": "Hermansson", "given": "Malte", "initials": "M"}], "type": "journal article", "published": "2019-03-25", "journal": {"volume": "9", "issn": "2045-2322", "issue": "1", "pages": "5110", "title": "Sci Rep", "issn-l": "2045-2322"}, "abstract": "Microbial biofilms are ubiquitous in aquatic environments where they provide important ecosystem functions. A key property believed to influence the community structure and function of biofilms is thickness. However, since biofilm thickness is inextricably linked to external factors such as water flow, temperature, development age and nutrient conditions, its importance is difficult to quantify. Here, we designed an experimental system in a wastewater treatment plant whereby nitrifying biofilms with different thicknesses (50 or 400 \u00b5m) were grown in a single reactor, and thus subjected to identical external conditions. The 50 and 400 \u00b5m biofilm communities were significantly different. This beta-diversity between biofilms of different thickness was primarily caused by deterministic factors. Turnover (species replacement) contributed more than nestedness (species loss) to the beta-diversity, i.e. the 50 \u00b5m communities were not simply a subset of the 400 \u00b5m communities. Moreover, the two communities differed in the composition of nitrogen-transforming bacteria and in nitrogen transformation rates. The study illustrates that biofilm thickness alone is a key driver for community composition and ecosystem function, which has implications for biotechnological applications and for our general understanding of biofilm ecology.", "doi": "10.1038/s41598-019-41542-1", "pmid": "30911066", "labels": {"Integrated Microscopy Technologies Gothenburg": "Service"}, "xrefs": [{"db": "pii", "key": "10.1038/s41598-019-41542-1"}, {"db": "pmc", "key": "PMC6434030"}], "notes": [], "created": "2020-01-23T16:13:32.374Z", "modified": "2021-06-16T14:44:51.298Z"}]}