{"entity": "researcher", "timestamp": "2026-08-07T19:35:10.277Z", "family": "Guo", "given": "Yongzhi", "initials": "Y", "orcid": "0000-0002-5057-9095", "affiliations": ["Department of Clinical Sciences, Swedish University of Agricultural Sciences, P.O. Box 7023, SE-75007 Uppsala, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/8040d6c76eb6467585d054d55a1965fe.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/8040d6c76eb6467585d054d55a1965fe"}}, "publications": [{"entity": "publication", "iuid": "da70971f10de48a2a607c9b51dc45c38", "links": {"self": {"href": "https://publications.scilifelab.se/publication/da70971f10de48a2a607c9b51dc45c38.json"}, "display": {"href": "https://publications.scilifelab.se/publication/da70971f10de48a2a607c9b51dc45c38"}}, "title": "Enrichment of Cis-Acting Regulatory Elements in Differentially Methylated Regions Following Lipopolysaccharide Treatment of Bovine Endometrial Epithelial Cells.", "authors": [{"family": "Jhamat", "given": "Naveed", "initials": "N", "orcid": "0009-0002-9876-4262", "researcher": {"href": "https://publications.scilifelab.se/researcher/e95627ce396b48f3828f038706cad7ac.json"}}, {"family": "Guo", "given": "Yongzhi", "initials": "Y", "orcid": "0000-0002-5057-9095", "researcher": {"href": "https://publications.scilifelab.se/researcher/8040d6c76eb6467585d054d55a1965fe.json"}}, {"family": "Han", "given": "Jilong", "initials": "J", "orcid": "0000-0001-9747-8163", "researcher": {"href": "https://publications.scilifelab.se/researcher/6849bbc1472c447a82af373ca5a44e21.json"}}, {"family": "Humblot", "given": "Patrice", "initials": "P", "orcid": "0000-0002-5292-1798", "researcher": {"href": "https://publications.scilifelab.se/researcher/7d74c76f33864331ab7c72614ae855f0.json"}}, {"family": "Bongcam-Rudloff", "given": "Erik", "initials": "E", "orcid": "0000-0002-1947-8288", "researcher": {"href": "https://publications.scilifelab.se/researcher/6970ca57259d498588ecf9e1ad28a9b0.json"}}, {"family": "Andersson", "given": "G\u00f6ran", "initials": "G", "orcid": "0000-0001-5131-3144", "researcher": {"href": "https://publications.scilifelab.se/researcher/39ce81c314db47c8ad63c3ed38dffcb3.json"}}, {"family": "Niazi", "given": "Adnan", "initials": "A", "orcid": "0000-0003-0311-5279", "researcher": {"href": "https://publications.scilifelab.se/researcher/c9e07c9891804a60980eb07956a7cd0d.json"}}], "type": "journal article", "published": "2024-09-11", "journal": {"title": "Int J Mol Sci", "issn": "1422-0067", "volume": "25", "issue": "18", "issn-l": null}, "abstract": "Endometritis is an inflammatory disease that negatively influences fertility and is common in milk-producing cows. An in vitro model for bovine endometrial inflammation was used to identify enrichment of cis-acting regulatory elements in differentially methylated regions (DMRs) in the genome of in vitro-cultured primary bovine endometrial epithelial cells (bEECs) before and after treatment with lipopolysaccharide (LPS) from E. coli, a key player in the development of endometritis. The enriched regulatory elements contain binding sites for transcription factors with established roles in inflammation and hypoxia including NFKB and Hif-1\u03b1. We further showed co-localization of certain enriched cis-acting regulatory motifs including ARNT, Hif-1\u03b1, and NRF1. Our results show an intriguing interplay between increased mRNA levels in LPS-treated bEECs of the mRNAs encoding the key transcription factors such as AHR, EGR2, and STAT1, whose binding sites were enriched in the DMRs. Our results demonstrate an extraordinary cis-regulatory complexity in these DMRs having binding sites for both inflammatory and hypoxia-dependent transcription factors. Obtained data using this in vitro model for bacterial-induced endometrial inflammation have provided valuable information regarding key transcription factors relevant for clinical endometritis in both cattle and humans.", "doi": "10.3390/ijms25189832", "pmid": "39337320", "labels": {"NGI Short read": "Service", "NGI Uppsala (SNP&SEQ Technology Platform)": "Service", "National Genomics Infrastructure": "Service"}, "xrefs": [{"db": "pii", "key": "ijms25189832"}, {"db": "pmc", "key": "PMC11432661"}], "notes": [], "created": "2024-09-30T06:07:03.182Z", "modified": "2024-09-30T06:07:04.535Z"}, {"entity": "publication", "iuid": "55e97ce1989c4bb184fae3027bb2bf9c", "links": {"self": {"href": "https://publications.scilifelab.se/publication/55e97ce1989c4bb184fae3027bb2bf9c.json"}, "display": {"href": "https://publications.scilifelab.se/publication/55e97ce1989c4bb184fae3027bb2bf9c"}}, "title": "Differential gene expression in bovine endometrial epithelial cells after challenge with LPS; specific implications for genes involved in embryo maternal interactions.", "authors": [{"family": "Guo", "given": "Yongzhi", "initials": "Y", "orcid": "0000-0002-5057-9095", "researcher": {"href": "https://publications.scilifelab.se/researcher/8040d6c76eb6467585d054d55a1965fe.json"}}, {"family": "van Schaik", "given": "Tom", "initials": "T", "orcid": "0000-0001-7850-5074", "researcher": {"href": "https://publications.scilifelab.se/researcher/79ccfcbccc214a7591e33d611edf0e8a.json"}}, {"family": "Jhamat", "given": "Naveed", "initials": "N"}, {"family": "Niazi", "given": "Adnan", "initials": "A", "orcid": "0000-0003-0311-5279", "researcher": {"href": "https://publications.scilifelab.se/researcher/c9e07c9891804a60980eb07956a7cd0d.json"}}, {"family": "Chanrot", "given": "Metasu", "initials": "M"}, {"family": "Charpigny", "given": "Gilles", "initials": "G"}, {"family": "Valarcher", "given": "Jean Francois", "initials": "JF"}, {"family": "Bongcam-Rudloff", "given": "Erik", "initials": "E"}, {"family": "Andersson", "given": "G\u00f6ran", "initials": "G"}, {"family": "Humblot", "given": "Patrice", "initials": "P"}], "type": "journal article", "published": "2019-09-05", "journal": {"title": "PLoS ONE", "issn": "1932-6203", "volume": "14", "issue": "9", "pages": "e0222081", "issn-l": "1932-6203"}, "abstract": "Lipopolysaccharide (LPS) expressed on the surface of Gram-negative bacteria activates pro-inflammatory pathways, dys-regulates the function of endometrial cells and is a key player in the mechanisms involved in endometritis. This study aimed to investigate the effects of LPS on bovine endometrial epithelial cells (bEEC) from whole transcriptome with a special focus on genes involved in embryo-maternal interactions. Following in vitro culture, bEEC from three cows were exposed to 0, 2, and 8 \u03bcg/mL LPS for 24h. RNA samples extracted at 0 and 24 hours were analyzed by RNA sequencing (RNA-seq). At 24h, 2035 differentially expressed genes (DEGs) were identified between controls and samples treated with 2 \u03bcg/mL LPS. Gene ontology analysis showed that over-expressed DEGs were associated to immune response, response to stress and external stimuli, catalytic activity, and cell cycle. Genes associated with cell membrane and cell adhesion pathways were under-expressed. LPS induced changes in expression of specific genes related to embryo-maternal interactions including under-expression of eight members of the cadherin superfamily, over-expression of six members of the mucin family, and differential expression of a large set of genes binding the above molecules and of more than 20 transcripts coding for cytokines and their receptors. Type I interferon-\u03c4 dependent genes were also over-expressed. From a sub-set of 19 genes, (biological replicates of bEEC from cows taken at time 6 (n = 3), 24 (n = 6) and 48 hours (n = 3), and 2 technical replicates per sample) differential gene expression was confirmed by RT2-qPCR (r2 between fold changes at 24 hours by RT2-qPCR and RNA-seq = 0.97). These results indicate that LPS affects the function of bEEC in many ways by differential transcription, glycolytic metabolism and oxidative stress. Many transcriptomic signatures related to implantation and embryo maternal interactions were strongly affected by LPS. These results pave the way for further studies to investigate the duration of these changes and their possible impact on endometrial function and fertility.", "doi": "10.1371/journal.pone.0222081", "pmid": "31487323", "labels": {"NGI Short read": "Service", "NGI Uppsala (SNP&SEQ Technology Platform)": "Service", "National Genomics Infrastructure": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC6728075"}, {"db": "pii", "key": "PONE-D-19-15238"}], "notes": [], "created": "2024-09-30T06:07:45.782Z", "modified": "2024-09-30T06:07:46.035Z"}]}