{"entity": "researcher", "timestamp": "2026-07-17T17:15:13.778Z", "family": "Li", "given": "Qishuang", "initials": "Q", "orcid": "0000-0002-2148-7917", "affiliations": ["State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, 16 Neinanxiaojie, Dongcheng district, Beijing, China."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/7afb27a365e047039b7134b38461d4bc.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/7afb27a365e047039b7134b38461d4bc"}}, "publications": [{"entity": "publication", "iuid": "67f7141c96474ec1bb0e2619dd27f05f", "links": {"self": {"href": "https://publications.scilifelab.se/publication/67f7141c96474ec1bb0e2619dd27f05f.json"}, "display": {"href": "https://publications.scilifelab.se/publication/67f7141c96474ec1bb0e2619dd27f05f"}}, "title": "De novo production of protoberberine and benzophenanthridine alkaloids through metabolic engineering of yeast.", "authors": [{"family": "Jiao", "given": "Xiang", "initials": "X", "orcid": "0000-0001-8043-3719", "researcher": {"href": "https://publications.scilifelab.se/researcher/405364bbdf444b598c5bc13a3c6e4426.json"}}, {"family": "Fu", "given": "Xiaozhi", "initials": "X", "orcid": "0000-0002-4465-7528", "researcher": {"href": "https://publications.scilifelab.se/researcher/cdb3c34994ca41afa148487fc571aa8d.json"}}, {"family": "Li", "given": "Qishuang", "initials": "Q", "orcid": "0000-0002-2148-7917", "researcher": {"href": "https://publications.scilifelab.se/researcher/7afb27a365e047039b7134b38461d4bc.json"}}, {"family": "Bu", "given": "Junling", "initials": "J"}, {"family": "Liu", "given": "Xiuyu", "initials": "X"}, {"family": "Savolainen", "given": "Otto", "initials": "O"}, {"family": "Huang", "given": "Luqi", "initials": "L"}, {"family": "Guo", "given": "Juan", "initials": "J", "orcid": "0000-0002-1327-4428", "researcher": {"href": "https://publications.scilifelab.se/researcher/0f6b24fad40f4fe480512aa9da62682a.json"}}, {"family": "Nielsen", "given": "Jens", "initials": "J", "orcid": "0000-0002-9955-6003", "researcher": {"href": "https://publications.scilifelab.se/researcher/7a596e289be4438a8a2653b1f25fea8b.json"}}, {"family": "Chen", "given": "Yun", "initials": "Y", "orcid": "0000-0002-2146-6008", "researcher": {"href": "https://publications.scilifelab.se/researcher/c8a0c4c96b7e491496aa0991c7e9a460.json"}}], "type": "journal article", "published": "2024-10-09", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "15", "issue": "1", "pages": "8759", "issn-l": "2041-1723"}, "abstract": "Protoberberine alkaloids and benzophenanthridine alkaloids (BZDAs) are subgroups of benzylisoquinoline alkaloids (BIAs), which represent a diverse class of plant-specialized natural metabolites with many pharmacological properties. Microbial biosynthesis has been allowed for accessibility and scalable production of high-value BIAs. Here, we engineer Saccharomyces cerevisiae to de novo produce a series of protoberberines and BZDAs, including palmatine, berberine, chelerythrine, sanguinarine and chelirubine. An ER compartmentalization strategy is developed to improve vacuole protein berberine bridge enzyme (BBE) activity, resulting in >200% increase on the production of the key intermediate (S)-scoulerine. Another promiscuous vacuole protein dihydrobenzophenanthridine oxidase (DBOX) has been identified to catalyze two-electron oxidation on various tetrahydroprotoberberines at N7-C8 position and dihydrobenzophenanthridine alkaloids. Furthermore, cytosolically expressed DBOX can alleviate the limitation on BBE. This study highlights the potential of microbial cell factories for the biosynthesis of a diverse group of BIAs through engineering of heterologous plant enzymes.", "doi": "10.1038/s41467-024-53045-3", "pmid": "39384562", "labels": {"Integrated Microscopy Technologies Gothenburg": "Service", "Chalmers Mass Spectrometry Infrastructure": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC11464499"}, {"db": "pii", "key": "10.1038/s41467-024-53045-3"}], "notes": [], "created": "2024-11-15T12:07:09.645Z", "modified": "2024-11-27T15:29:12.299Z"}]}