{"entity": "researcher", "timestamp": "2026-07-17T07:36:23.841Z", "family": "Odell", "given": "Luke R", "initials": "LR", "orcid": "0000-0001-7658-5103", "affiliations": ["Department of Medicinal Chemistry, Uppsala University, Uppsala, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/7653ba79ce9d4e4a80be4b42f516680b.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/7653ba79ce9d4e4a80be4b42f516680b"}}, "publications": [{"entity": "publication", "iuid": "2a366cbffd9d403f89718e65c9e2b1a0", "links": {"self": {"href": "https://publications.scilifelab.se/publication/2a366cbffd9d403f89718e65c9e2b1a0.json"}, "display": {"href": "https://publications.scilifelab.se/publication/2a366cbffd9d403f89718e65c9e2b1a0"}}, "title": "Hydrazide-based reactive matrices for the sensitive detection of aldehydes and ketones by MALDI mass spectrometry imaging.", "authors": [{"family": "Lod\u00e9n", "given": "Henrik", "initials": "H", "orcid": "0000-0002-2416-5251", "researcher": {"href": "https://publications.scilifelab.se/researcher/bd5349a708e242769091df471639f5b6.json"}}, {"family": "Schembri", "given": "Luke S", "initials": "LS", "orcid": "0000-0001-5588-1077", "researcher": {"href": "https://publications.scilifelab.se/researcher/9dc760b581324125816ed40eb748104c.json"}}, {"family": "Nilsson", "given": "Anna", "initials": "A"}, {"family": "Kaya", "given": "Ibrahim", "initials": "I"}, {"family": "Shariatgorji", "given": "Reza", "initials": "R"}, {"family": "Odell", "given": "Luke R", "initials": "LR", "orcid": "0000-0001-7658-5103", "researcher": {"href": "https://publications.scilifelab.se/researcher/7653ba79ce9d4e4a80be4b42f516680b.json"}}, {"family": "Andr\u00e9n", "given": "Per E", "initials": "PE", "orcid": "0000-0002-4062-7743", "researcher": {"href": "https://publications.scilifelab.se/researcher/64f6381de42949db8d30b56b526f3e26.json"}}], "type": "journal article", "published": "2024-08-22", "journal": {"title": "Chem. Commun. (Camb.)", "issn": "1364-548X", "volume": "60", "issue": "69", "pages": "9238-9241", "issn-l": "1359-7345"}, "abstract": "A one-step, on-tissue chemical derivatisation method for MALDI mass spectrometry imaging was found to improve the detectability of aldehydes and ketones by charge-tagging. The developed reactive matrices, containing a UV-chromophore, ionisable moiety and hydrazide group, showed an equal or higher detection efficiency than Girard's reagent P, enabling improved imaging of brain metabolites without the need for additional co-matrices.", "doi": "10.1039/d4cc02475c", "pmid": "39114958", "labels": {"Spatial Mass Spectrometry": "Technology development"}, "xrefs": [], "notes": [], "created": "2024-11-20T10:38:02.805Z", "modified": "2024-11-20T10:40:23.399Z"}, {"entity": "publication", "iuid": "012eb27f39fb47bfa72235c450265967", "links": {"self": {"href": "https://publications.scilifelab.se/publication/012eb27f39fb47bfa72235c450265967.json"}, "display": {"href": "https://publications.scilifelab.se/publication/012eb27f39fb47bfa72235c450265967"}}, "title": "A Base\u2010Free, Low Temperature Click and Release Reaction for the In Situ Generation of Diazomethane", "authors": [{"family": "Schembri", "given": "Luke S", "initials": "LS", "orcid": "0000-0001-5588-1077", "researcher": {"href": "https://publications.scilifelab.se/researcher/9dc760b581324125816ed40eb748104c.json"}}, {"family": "Olaniran", "given": "Esther", "initials": "E"}, {"family": "S\u00f6derstr\u00f6m", "given": "Marcus", "initials": "M"}, {"family": "Skillinghaug", "given": "Bobo", "initials": "B"}, {"family": "Odell", "given": "Luke R", "initials": "LR", "orcid": "0000-0001-7658-5103", "researcher": {"href": "https://publications.scilifelab.se/researcher/7653ba79ce9d4e4a80be4b42f516680b.json"}}], "type": "journal-article", "published": "2023-06-13", "journal": {"title": "Adv. Synth. Catal.", "issn": "1615-4150", "volume": "365", "issue": "11", "pages": "1839-1845", "issn-l": null}, "abstract": null, "doi": "10.1002/adsc.202300132", "pmid": null, "labels": {"Spatial Mass Spectrometry": "Service"}, "xrefs": [], "notes": [], "created": "2023-11-30T14:43:11.826Z", "modified": "2025-12-01T07:51:50.302Z"}, {"entity": "publication", "iuid": "0e83e82f332d45cc99e0bc033b1c90c2", "links": {"self": {"href": "https://publications.scilifelab.se/publication/0e83e82f332d45cc99e0bc033b1c90c2.json"}, "display": {"href": "https://publications.scilifelab.se/publication/0e83e82f332d45cc99e0bc033b1c90c2"}}, "title": "On-Tissue Chemical Derivatization for Comprehensive Mapping of Brain Carboxyl and Aldehyde Metabolites by MALDI-MS Imaging.", "authors": [{"family": "Kaya", "given": "Ibrahim", "initials": "I"}, {"family": "Schembri", "given": "Luke S", "initials": "LS", "orcid": "0000-0001-5588-1077", "researcher": {"href": "https://publications.scilifelab.se/researcher/9dc760b581324125816ed40eb748104c.json"}}, {"family": "Nilsson", "given": "Anna", "initials": "A"}, {"family": "Shariatgorji", "given": "Reza", "initials": "R", "orcid": "0000-0001-9484-0921", "researcher": {"href": "https://publications.scilifelab.se/researcher/7762e9f6779c4780a4077c557eb7a3b6.json"}}, {"family": "Baijnath", "given": "Sooraj", "initials": "S", "orcid": "0000-0001-7860-1779", "researcher": {"href": "https://publications.scilifelab.se/researcher/a918cd14121f40bf88ad33082921e5c2.json"}}, {"family": "Zhang", "given": "Xiaoqun", "initials": "X"}, {"family": "Bezard", "given": "Erwan", "initials": "E"}, {"family": "Svenningsson", "given": "Per", "initials": "P"}, {"family": "Odell", "given": "Luke R", "initials": "LR", "orcid": "0000-0001-7658-5103", "researcher": {"href": "https://publications.scilifelab.se/researcher/7653ba79ce9d4e4a80be4b42f516680b.json"}}, {"family": "Andr\u00e9n", "given": "Per E", "initials": "PE", "orcid": "0000-0002-4062-7743", "researcher": {"href": "https://publications.scilifelab.se/researcher/64f6381de42949db8d30b56b526f3e26.json"}}], "type": "journal article", "published": "2023-05-03", "journal": {"title": "J. Am. Soc. Mass Spectrom.", "issn": "1879-1123", "volume": "34", "issue": "5", "pages": "836-846", "issn-l": "1044-0305"}, "abstract": "The visualization of small metabolites by MALDI mass spectrometry imaging in brain tissue sections is challenging due to low detection sensitivity and high background interference. We present an on-tissue chemical derivatization MALDI mass spectrometry imaging approach for the comprehensive mapping of carboxyls and aldehydes in brain tissue sections. In this approach, the AMPP (1-(4-(aminomethyl)phenyl)pyridin-1-ium chloride) derivatization reagent is used for the covalent charge-tagging of molecules containing carboxylic acid (in the presence of peptide coupling reagents) and aldehydes. This includes free fatty acids and the associated metabolites, fatty aldehydes, dipeptides, neurotoxic reactive aldehydes, amino acids, neurotransmitters and associated metabolites, as well as tricarboxylic acid cycle metabolites. We performed sensitive ultrahigh mass resolution MALDI-MS detection and imaging of various carboxyl- and aldehyde-containing endogenous metabolites simultaneously in rodent brain tissue sections. We verified the AMPP-derivatized metabolites by tandem MS for structural elucidation. This approach allowed us to image numerous aldehydes and carboxyls, including certain metabolites which had been undetectable in brain tissue sections. We also demonstrated the application of on-tissue derivatization to carboxyls and aldehydes in coronal brain tissue sections of a nonhuman primate Parkinson's disease model. Our methodology provides a powerful tool for the sensitive, simultaneous spatial molecular imaging of numerous aldehydes and carboxylic acids during pathological states, including neurodegeneration, in brain tissue.", "doi": "10.1021/jasms.2c00336", "pmid": "37052344", "labels": {"Spatial Mass Spectrometry": "Technology development"}, "xrefs": [{"db": "pmc", "key": "PMC10161219"}], "notes": [], "created": "2023-11-30T10:16:33.021Z", "modified": "2023-11-30T10:16:33.076Z"}, {"entity": "publication", "iuid": "427143398ef547e2b13bbc005c229189", "links": {"self": {"href": "https://publications.scilifelab.se/publication/427143398ef547e2b13bbc005c229189.json"}, "display": {"href": "https://publications.scilifelab.se/publication/427143398ef547e2b13bbc005c229189"}}, "title": "Region-Specific and Age-Dependent Multitarget Effects of Acetylcholinesterase Inhibitor Tacrine on Comprehensive Neurotransmitter Systems.", "authors": [{"family": "Fridjonsdottir", "given": "Elva", "initials": "E"}, {"family": "Vallianatou", "given": "Theodosia", "initials": "T", "orcid": "0000-0002-1477-7756", "researcher": {"href": "https://publications.scilifelab.se/researcher/ae610b7669754328a119654fdfcd6af4.json"}}, {"family": "Mantas", "given": "Ioannis", "initials": "I"}, {"family": "Shariatgorji", "given": "Reza", "initials": "R", "orcid": "0000-0001-9484-0921", "researcher": {"href": "https://publications.scilifelab.se/researcher/7762e9f6779c4780a4077c557eb7a3b6.json"}}, {"family": "Nilsson", "given": "Anna", "initials": "A"}, {"family": "Schembri", "given": "Luke S", "initials": "LS", "orcid": "0000-0001-5588-1077", "researcher": {"href": "https://publications.scilifelab.se/researcher/9dc760b581324125816ed40eb748104c.json"}}, {"family": "Odell", "given": "Luke R", "initials": "LR", "orcid": "0000-0001-7658-5103", "researcher": {"href": "https://publications.scilifelab.se/researcher/7653ba79ce9d4e4a80be4b42f516680b.json"}}, {"family": "Svenningsson", "given": "Per", "initials": "P"}, {"family": "Andr\u00e9n", "given": "Per E", "initials": "PE", "orcid": "0000-0002-4062-7743", "researcher": {"href": "https://publications.scilifelab.se/researcher/64f6381de42949db8d30b56b526f3e26.json"}}], "type": "journal article", "published": "2022-01-21", "journal": {"title": "ACS Chem. Biol.", "issn": "1554-8937", "volume": "17", "issue": "1", "pages": "147-158", "issn-l": "1554-8929"}, "abstract": "Regional brain distribution and metabolism of neurotransmitters and their response to drug treatment are fundamentally important for understanding the central effects of neuroactive substances. We used matrix-assisted laser desorption/ionization mass spectrometry imaging in combination with multivariate analysis to visualize in anatomical detail metabolic effects of aging and tacrine-mediated acetylcholinesterase inhibition on comprehensive neurotransmitter systems in multiple mouse brain regions of 12-week-old and 14-month-old mice. We detected age-related increases in 3,4-dihydroxyphenylacetaldehyde and histamine, indicating oxidative stress and aging deficits in astrocytes. Tacrine had a significant impact on the metabolism of neurotransmitters in both age groups; predominantly, there was an increased norepinephrine turnover throughout the brain and decreased 3-methoxy tyramine, a marker for dopamine release, in the striatum. The striatal levels of histamine were only elevated after tacrine administration in the older animals. Our results demonstrated that tacrine is a multitarget and region-specific neuroactive agent, inducing age-specific responses. Although well-studied, the complete mechanisms of the action of tacrine are not fully understood, and the current findings reveal features that may help explain its treatment-related effectiveness and central side effects.", "doi": "10.1021/acschembio.1c00803", "pmid": "34932310", "labels": {"Spatial Mass Spectrometry": "Technology development"}, "xrefs": [{"db": "pmc", "key": "PMC8787750"}], "notes": [], "created": "2022-11-16T14:01:55.350Z", "modified": "2022-11-16T14:01:55.392Z"}, {"entity": "publication", "iuid": "aab5be8b28f949029e6e9dafb461eca8", "links": {"self": {"href": "https://publications.scilifelab.se/publication/aab5be8b28f949029e6e9dafb461eca8.json"}, "display": {"href": "https://publications.scilifelab.se/publication/aab5be8b28f949029e6e9dafb461eca8"}}, "title": "Mass spectrometry imaging identifies abnormally elevated brain l-DOPA levels and extrastriatal monoaminergic dysregulation in l-DOPA-induced dyskinesia.", "authors": [{"family": "Fridjonsdottir", "given": "Elva", "initials": "E", "orcid": "0000-0002-3779-3556", "researcher": {"href": "https://publications.scilifelab.se/researcher/d39a6e68683d4414bb87a0dc7033a7a4.json"}}, {"family": "Shariatgorji", "given": "Reza", "initials": "R", "orcid": "0000-0001-9484-0921", "researcher": {"href": "https://publications.scilifelab.se/researcher/7762e9f6779c4780a4077c557eb7a3b6.json"}}, {"family": "Nilsson", "given": "Anna", "initials": "A", "orcid": "0000-0002-6183-6931", "researcher": {"href": "https://publications.scilifelab.se/researcher/79c9fe163d31414e8b66aa0834538627.json"}}, {"family": "Vallianatou", "given": "Theodosia", "initials": "T", "orcid": "0000-0002-1477-7756", "researcher": {"href": "https://publications.scilifelab.se/researcher/ae610b7669754328a119654fdfcd6af4.json"}}, {"family": "Odell", "given": "Luke R", "initials": "LR", "orcid": "0000-0001-7658-5103", "researcher": {"href": "https://publications.scilifelab.se/researcher/7653ba79ce9d4e4a80be4b42f516680b.json"}}, {"family": "Schembri", "given": "Luke S", "initials": "LS", "orcid": "0000-0001-5588-1077", "researcher": {"href": "https://publications.scilifelab.se/researcher/9dc760b581324125816ed40eb748104c.json"}}, {"family": "Svenningsson", "given": "Per", "initials": "P", "orcid": "0000-0001-6727-3802", "researcher": {"href": "https://publications.scilifelab.se/researcher/5199496295334771ba3c5621a83a6f43.json"}}, {"family": "Fernagut", "given": "Pierre-Olivier", "initials": "P"}, {"family": "Crossman", "given": "Alan R", "initials": "AR"}, {"family": "Bezard", "given": "Erwan", "initials": "E", "orcid": "0000-0002-0410-4638", "researcher": {"href": "https://publications.scilifelab.se/researcher/c33e3029113549ea96e60c7952e5a19a.json"}}, {"family": "Andr\u00e9n", "given": "Per E", "initials": "PE", "orcid": "0000-0002-4062-7743", "researcher": {"href": "https://publications.scilifelab.se/researcher/64f6381de42949db8d30b56b526f3e26.json"}}], "type": "journal article", "published": "2021-01-00", "journal": {"title": "Sci Adv", "issn": "2375-2548", "issn-l": "2375-2548", "volume": "7", "issue": "2", "pages": null}, "abstract": "l-DOPA treatment for Parkinson's disease frequently leads to dyskinesias, the pathophysiology of which is poorly understood. We used MALDI-MSI to map the distribution of l-DOPA and monoaminergic pathways in brains of dyskinetic and nondyskinetic primates. We report elevated levels of l-DOPA, and its metabolite 3-O-methyldopa, in all measured brain regions of dyskinetic animals and increases in dopamine and metabolites in all regions analyzed except the striatum. In dyskinesia, dopamine levels correlated well with l-DOPA levels in extrastriatal regions, such as hippocampus, amygdala, bed nucleus of the stria terminalis, and cortical areas, but not in the striatum. Our results demonstrate that l-DOPA-induced dyskinesia is linked to a dysregulation of l-DOPA metabolism throughout the brain. The inability of extrastriatal brain areas to regulate the formation of dopamine during l-DOPA treatment introduces the potential of dopamine or even l-DOPA itself to modulate neuronal signaling widely across the brain, resulting in unwanted side effects.", "doi": "10.1126/sciadv.abe5948", "pmid": "33523980", "labels": {"Spatial Mass Spectrometry": "Technology development"}, "xrefs": [{"db": "pii", "key": "7/2/eabe5948"}, {"db": "pmc", "key": "PMC7787486"}], "notes": [], "created": "2021-03-26T12:47:07.296Z", "modified": "2024-02-09T14:58:49.154Z"}, {"entity": "publication", "iuid": "833bd35c6f9343eca70e9d216f2dac9a", "links": {"self": {"href": "https://publications.scilifelab.se/publication/833bd35c6f9343eca70e9d216f2dac9a.json"}, "display": {"href": "https://publications.scilifelab.se/publication/833bd35c6f9343eca70e9d216f2dac9a"}}, "title": "Rational Design of Azastatin as a Potential ADC Payload with Reduced Bystander Killing.", "authors": [{"family": "Hartmann", "given": "Rafael W", "initials": "RW", "orcid": "0000-0002-5520-0179", "researcher": {"href": "https://publications.scilifelab.se/researcher/7c8d3d38aa874be68232147db4b1dde0.json"}}, {"family": "Fahrner", "given": "Raphael", "initials": "R", "orcid": "0000-0002-6737-0917", "researcher": {"href": "https://publications.scilifelab.se/researcher/4bff9aa2f39b428599d006065f0400d9.json"}}, {"family": "Shevshenko", "given": "Denys", "initials": "D", "orcid": "0000-0001-8779-0153", "researcher": {"href": "https://publications.scilifelab.se/researcher/fe6b3911262c4355af202746e3be90b7.json"}}, {"family": "Fyrkn\u00e4s", "given": "M\u00e5rten", "initials": "M"}, {"family": "Larsson", "given": "Rolf", "initials": "R"}, {"family": "Lehmann", "given": "Fredrik", "initials": "F", "orcid": "0000-0001-7385-3870", "researcher": {"href": "https://publications.scilifelab.se/researcher/822d2043ec8c4d3bb5341a1dfd007d4e.json"}}, {"family": "Odell", "given": "Luke R", "initials": "LR", "orcid": "0000-0001-7658-5103", "researcher": {"href": "https://publications.scilifelab.se/researcher/7653ba79ce9d4e4a80be4b42f516680b.json"}}], "type": "journal article", "published": "2020-12-15", "journal": {"title": "ChemMedChem", "issn": "1860-7187", "volume": "15", "issue": "24", "pages": "2500-2512", "issn-l": "1860-7179"}, "abstract": "Auristatins are a class of ultrapotent microtubule inhibitors, whose growing clinical popularity in oncology is based upon their use as payloads in antibody-drug conjugates (ADCs). The most widely utilized auristatin, MMAE, has however been shown to cause apoptosis in non-pathological cells proximal to the tumour (\"bystander killing\"). Herein, we introduce azastatins, a new class of auristatin derivatives encompassing a side chain amine for antibody conjugation. The synthesis of Cbz-azastatin methyl ester, which included the C2-elongation and diastereoselective reduction of two proteinogenic amino acids as key transformations, was accomplished in 22 steps and 0.76 % overall yield. While Cbz-protected azastatin methyl ester (0.13-3.0 nM) inhibited proliferation more potently than MMAE (0.47-6.5 nM), removal of the Cbz-group yielded dramatically increased IC50 -values (9.8-170 nM). We attribute the reduced apparent cytotoxicity of the deprotected azastatin methyl esters to a lack of membrane permeability. These results clearly establish the azastatins as a novel class of cytotoxic payloads ideally suited for use in next-generation ADC development.", "doi": "10.1002/cmdc.202000497", "pmid": "33063934", "labels": {"Drug Discovery and Development": null}, "xrefs": [{"db": "pmc", "key": "PMC7756782"}], "notes": [], "created": "2021-12-08T12:26:21.225Z", "modified": "2025-10-17T13:05:07.940Z"}, {"entity": "publication", "iuid": "2bb8023f2c4d46a08d765987bffeadb4", "links": {"self": {"href": "https://publications.scilifelab.se/publication/2bb8023f2c4d46a08d765987bffeadb4.json"}, "display": {"href": "https://publications.scilifelab.se/publication/2bb8023f2c4d46a08d765987bffeadb4"}}, "title": "Comprehensive mapping of neurotransmitter networks by MALDI-MS imaging.", "authors": [{"family": "Shariatgorji", "given": "Mohammadreza", "initials": "M"}, {"family": "Nilsson", "given": "Anna", "initials": "A"}, {"family": "Fridjonsdottir", "given": "Elva", "initials": "E", "orcid": "0000-0002-3779-3556", "researcher": {"href": "https://publications.scilifelab.se/researcher/d39a6e68683d4414bb87a0dc7033a7a4.json"}}, {"family": "Vallianatou", "given": "Theodosia", "initials": "T"}, {"family": "K\u00e4llback", "given": "Patrik", "initials": "P"}, {"family": "Katan", "given": "Luay", "initials": "L"}, {"family": "S\u00e4vmarker", "given": "Jonas", "initials": "J"}, {"family": "Mantas", "given": "Ioannis", "initials": "I"}, {"family": "Zhang", "given": "Xiaoqun", "initials": "X"}, {"family": "Bezard", "given": "Erwan", "initials": "E", "orcid": "0000-0002-0410-4638", "researcher": {"href": "https://publications.scilifelab.se/researcher/c33e3029113549ea96e60c7952e5a19a.json"}}, {"family": "Svenningsson", "given": "Per", "initials": "P"}, {"family": "Odell", "given": "Luke R", "initials": "LR", "orcid": "0000-0001-7658-5103", "researcher": {"href": "https://publications.scilifelab.se/researcher/7653ba79ce9d4e4a80be4b42f516680b.json"}}, {"family": "Andr\u00e9n", "given": "Per E", "initials": "PE", "orcid": "0000-0002-4062-7743", "researcher": {"href": "https://publications.scilifelab.se/researcher/64f6381de42949db8d30b56b526f3e26.json"}}], "type": "journal article", "published": "2019-10-00", "journal": {"title": "Nat. Methods", "issn": "1548-7105", "volume": "16", "issue": "10", "pages": "1021-1028", "issn-l": "1548-7091"}, "abstract": "We present a mass spectrometry imaging (MSI) approach for the comprehensive mapping of neurotransmitter networks in specific brain regions. Our fluoromethylpyridinium-based reactive matrices facilitate the covalent charge-tagging of molecules containing phenolic hydroxyl and/or primary or secondary amine groups, including dopaminergic and serotonergic neurotransmitters and their associated metabolites. These matrices improved the matrix-assisted laser desorption/ionization (MALDI)-MSI detection limit toward low-abundance neurotransmitters and facilitated the simultaneous imaging of neurotransmitters in fine structures of the brain at a lateral resolution of 10 \u00b5m. We demonstrate strategies for the identification of unknown molecular species using the innate chemoselectivity of the reactive matrices and the unique isotopic pattern of a brominated reactive matrix. We illustrate the capabilities of the developed method on Parkinsonian brain samples from human post-mortem tissue and animal models. The direct imaging of neurotransmitter systems provides a method for exploring how various neurological diseases affect specific brain regions through neurotransmitter modulation.", "doi": "10.1038/s41592-019-0551-3", "pmid": "31548706", "labels": {"Spatial Mass Spectrometry": "Technology development"}, "xrefs": [{"db": "pii", "key": "10.1038/s41592-019-0551-3"}], "notes": [], "created": "2020-01-24T09:24:21.630Z", "modified": "2021-12-03T11:53:56.410Z"}]}