{"entity": "researcher", "timestamp": "2026-07-12T08:45:28.622Z", "family": "Robert", "given": "St\u00e9phanie", "initials": "S", "orcid": "0000-0002-0013-3239", "affiliations": ["Ume\u00e5 Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, SE-901 83, Ume\u00e5, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/74a96c43520f4c9c91e44be8a7d39e6c.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/74a96c43520f4c9c91e44be8a7d39e6c"}}, "publications": [{"entity": "publication", "iuid": "acb48c2531964cb4b5a8c73639e2f114", "links": {"self": {"href": "https://publications.scilifelab.se/publication/acb48c2531964cb4b5a8c73639e2f114.json"}, "display": {"href": "https://publications.scilifelab.se/publication/acb48c2531964cb4b5a8c73639e2f114"}}, "title": "Cell-surface receptors enable perception of extracellular cytokinins.", "authors": [{"family": "Antoniadi", "given": "Ioanna", "initials": "I", "orcid": "0000-0001-9053-2788", "researcher": {"href": "https://publications.scilifelab.se/researcher/60bcbb474ded4df08079520c92de49bf.json"}}, {"family": "Nov\u00e1k", "given": "Ond\u0159ej", "initials": "O", "orcid": "0000-0003-3452-0154", "researcher": {"href": "https://publications.scilifelab.se/researcher/8c19165acb9a4ff79dd96af7fccdc5f8.json"}}, {"family": "Gelov\u00e1", "given": "Zuzana", "initials": "Z", "orcid": "0000-0003-4783-1752", "researcher": {"href": "https://publications.scilifelab.se/researcher/afc862721b52467aa092f0ecf9fc7bed.json"}}, {"family": "Johnson", "given": "Alexander", "initials": "A", "orcid": "0000-0002-2739-8843", "researcher": {"href": "https://publications.scilifelab.se/researcher/c9414092144944429b428466733e6567.json"}}, {"family": "Pl\u00edhal", "given": "Ond\u0159ej", "initials": "O", "orcid": "0000-0003-0645-2019", "researcher": {"href": "https://publications.scilifelab.se/researcher/f42115be2a6b4268a3bf6f9200479f93.json"}}, {"family": "Simersk\u00fd", "given": "Radim", "initials": "R", "orcid": "0000-0002-4481-6680", "researcher": {"href": "https://publications.scilifelab.se/researcher/218c11787cf2467a98ab7d0c826de187.json"}}, {"family": "Mik", "given": "V\u00e1clav", "initials": "V", "orcid": "0000-0002-8515-3035", "researcher": {"href": "https://publications.scilifelab.se/researcher/51f69a95dba24bd793e708f0cdcc2ded.json"}}, {"family": "Vain", "given": "Thomas", "initials": "T"}, {"family": "Mateo-Bonmat\u00ed", "given": "Eduardo", "initials": "E", "orcid": "0000-0002-2364-5173", "researcher": {"href": "https://publications.scilifelab.se/researcher/f1e96edf1a914d2f8ea8eb46c3ebacc4.json"}}, {"family": "Karady", "given": "Michal", "initials": "M"}, {"family": "Pernisov\u00e1", "given": "Mark\u00e9ta", "initials": "M", "orcid": "0000-0002-5803-2879", "researcher": {"href": "https://publications.scilifelab.se/researcher/acc243598b74495999230f9b1b0da0be.json"}}, {"family": "Pla\u010dkov\u00e1", "given": "Lenka", "initials": "L"}, {"family": "Opassathian", "given": "Korawit", "initials": "K"}, {"family": "Hej\u00e1tko", "given": "Jan", "initials": "J"}, {"family": "Robert", "given": "St\u00e9phanie", "initials": "S", "orcid": "0000-0002-0013-3239", "researcher": {"href": "https://publications.scilifelab.se/researcher/74a96c43520f4c9c91e44be8a7d39e6c.json"}}, {"family": "Friml", "given": "Ji\u0159\u00ed", "initials": "J", "orcid": "0000-0002-8302-7596", "researcher": {"href": "https://publications.scilifelab.se/researcher/6e525fe6afc5490a86223e38be195cee.json"}}, {"family": "Dole\u017eal", "given": "Karel", "initials": "K", "orcid": "0000-0003-4938-0350", "researcher": {"href": "https://publications.scilifelab.se/researcher/f209cf1d8e5d4945b81bd9f01c2b2665.json"}}, {"family": "Ljung", "given": "Karin", "initials": "K", "orcid": "0000-0003-2901-189X", "researcher": {"href": "https://publications.scilifelab.se/researcher/f91b1e1f90c24559b915ebcd265804a4.json"}}, {"family": "Turnbull", "given": "Colin", "initials": "C", "orcid": "0000-0001-6635-1418", "researcher": {"href": "https://publications.scilifelab.se/researcher/abd75adef49f4c789b69206cdb7e8cf0.json"}}], "type": "journal article", "published": "2020-08-27", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "11", "issue": "1", "pages": "4284", "issn-l": "2041-1723"}, "abstract": "Cytokinins are mobile multifunctional plant hormones with roles in development and stress resilience. Although their Histidine Kinase receptors are substantially localised to the endoplasmic reticulum, cellular sites of cytokinin perception and importance of spatially heterogeneous cytokinin distribution continue to be debated. Here we show that cytokinin perception by plasma membrane receptors is an effective additional path for cytokinin response. Readout from a Two Component Signalling cytokinin-specific reporter (TCSn::GFP) closely matches intracellular cytokinin content in roots, yet we also find cytokinins in extracellular fluid, potentially enabling action at the cell surface. Cytokinins covalently linked to beads that could not pass the plasma membrane increased expression of both TCSn::GFP and Cytokinin Response Factors. Super-resolution microscopy of GFP-labelled receptors and diminished TCSn::GFP response to immobilised cytokinins in cytokinin receptor mutants, further indicate that receptors can function at the cell surface. We argue that dual intracellular and surface locations may augment flexibility of cytokinin responses.", "doi": "10.1038/s41467-020-17700-9", "pmid": "32855409", "labels": {"Swedish Metabolomics Centre": "Service"}, "xrefs": [{"db": "pii", "key": "10.1038/s41467-020-17700-9"}, {"db": "pmc", "key": "PMC7453015"}], "notes": [], "created": "2020-12-11T11:56:05.497Z", "modified": "2025-10-17T13:03:16.685Z"}, {"entity": "publication", "iuid": "33658aaead7a401091083bf538938f14", "links": {"self": {"href": "https://publications.scilifelab.se/publication/33658aaead7a401091083bf538938f14.json"}, "display": {"href": "https://publications.scilifelab.se/publication/33658aaead7a401091083bf538938f14"}}, "title": "Chemical Screening Pipeline for Identification of Specific Plant Autophagy Modulators.", "authors": [{"family": "Dauphinee", "given": "Adrian N", "initials": "AN", "orcid": "0000-0002-3363-198X", "researcher": {"href": "https://publications.scilifelab.se/researcher/ee402a3843fb47f894129d05e432e132.json"}}, {"family": "Cardoso", "given": "Catarina", "initials": "C", "orcid": "0000-0003-3323-4447", "researcher": {"href": "https://publications.scilifelab.se/researcher/6804b1e09cdc4ded859259ccaaebf3a3.json"}}, {"family": "Dalman", "given": "Kerstin", "initials": "K", "orcid": "0000-0002-9919-9149", "researcher": {"href": "https://publications.scilifelab.se/researcher/dcaac86d01994ea3b2059984412f1e9a.json"}}, {"family": "Ohlsson", "given": "Jonas A", "initials": "JA", "orcid": "0000-0001-8598-2899", "researcher": {"href": "https://publications.scilifelab.se/researcher/a5ce6fe7d5ff4071986fe1cf286b2f8a.json"}}, {"family": "Fick", "given": "Stina Berglund", "initials": "SB", "orcid": "0000-0003-4968-8830", "researcher": {"href": "https://publications.scilifelab.se/researcher/29ba8a613d394ef4bd333eb052219035.json"}}, {"family": "Robert", "given": "St\u00e9phanie", "initials": "S", "orcid": "0000-0002-0013-3239", "researcher": {"href": "https://publications.scilifelab.se/researcher/74a96c43520f4c9c91e44be8a7d39e6c.json"}}, {"family": "Hicks", "given": "Glenn R", "initials": "GR", "orcid": "0000-0002-2502-7782", "researcher": {"href": "https://publications.scilifelab.se/researcher/aca704d8180549e4a4786fcae24172a8.json"}}, {"family": "Bozhkov", "given": "Peter V", "initials": "PV", "orcid": "0000-0002-8819-3884", "researcher": {"href": "https://publications.scilifelab.se/researcher/858ffb21080f4b75ae982f76ade8d742.json"}}, {"family": "Minina", "given": "Elena A", "initials": "EA", "orcid": "0000-0002-2619-1859", "researcher": {"href": "https://publications.scilifelab.se/researcher/63cb7fc5ae9348ea97ce21d5f86ad623.json"}}], "type": "journal article", "published": "2019-11-00", "journal": {"title": "Plant Physiol.", "issn": "1532-2548", "issn-l": "0032-0889", "volume": "181", "issue": "3", "pages": "855-866"}, "abstract": "Autophagy is a major catabolic process in eukaryotes with a key role in homeostasis, programmed cell death, and aging. In plants, autophagy is also known to regulate agronomically important traits such as stress resistance, longevity, vegetative biomass, and seed yield. Despite its significance, there is still a shortage of reliable tools modulating plant autophagy. Here, we describe the first robust pipeline for identification of specific plant autophagy-modulating compounds. Our screening protocol comprises four phases: (1) high-throughput screening of chemical compounds in cell cultures of tobacco (Nicotiana tabacum); (2) confirmation of the identified hits in planta using Arabidopsis (Arabidopsis thaliana); (3) further characterization of the effect using conventional molecular biology methods; and (4) verification of chemical specificity on autophagy in planta. The methods detailed here streamline the identification of specific plant autophagy modulators and aid in unraveling the molecular mechanisms of plant autophagy.", "doi": "10.1104/pp.19.00647", "pmid": "31488572", "labels": {"Chemical Biology Consortium Sweden": "Collaborative"}, "xrefs": [{"db": "pii", "key": "pp.19.00647"}, {"db": "pmc", "key": "PMC6836817"}], "notes": [], "created": "2019-09-09T07:24:05.074Z", "modified": "2025-10-17T13:04:28.430Z"}, {"entity": "publication", "iuid": "a758a54e14db475586eb19b410a7141a", "links": {"self": {"href": "https://publications.scilifelab.se/publication/a758a54e14db475586eb19b410a7141a.json"}, "display": {"href": "https://publications.scilifelab.se/publication/a758a54e14db475586eb19b410a7141a"}}, "title": "A role for the auxin precursor anthranilic acid in root gravitropism via regulation of PIN-FORMED protein polarity and relocalisation in Arabidopsis.", "authors": [{"family": "Doyle", "given": "Siamsa M", "initials": "SM", "orcid": "0000-0003-4889-3496", "researcher": {"href": "https://publications.scilifelab.se/researcher/4a82fafd2ab34ff2bac338ce451bb4bb.json"}}, {"family": "Rigal", "given": "Adeline", "initials": "A"}, {"family": "Grones", "given": "Peter", "initials": "P", "orcid": "0000-0003-4132-4151", "researcher": {"href": "https://publications.scilifelab.se/researcher/20417b1485074106b74042617b5e2e59.json"}}, {"family": "Karady", "given": "Michal", "initials": "M", "orcid": "0000-0002-5603-706X", "researcher": {"href": "https://publications.scilifelab.se/researcher/114911ccd31849e1a7584b5a52e9eb14.json"}}, {"family": "Barange", "given": "Deepak K", "initials": "DK", "orcid": "0000-0003-1279-1068", "researcher": {"href": "https://publications.scilifelab.se/researcher/d00627926367423093b7e1645ee55ad1.json"}}, {"family": "Majda", "given": "Mateusz", "initials": "M", "orcid": "0000-0003-3405-2901", "researcher": {"href": "https://publications.scilifelab.se/researcher/582d7b364d4d4442a7f470e52ed031bc.json"}}, {"family": "Pa\u0159\u00edzkov\u00e1", "given": "Barbora", "initials": "B", "orcid": "0000-0002-8125-2271", "researcher": {"href": "https://publications.scilifelab.se/researcher/c9dd3d0c062245a5b188e774955cd210.json"}}, {"family": "Karampelias", "given": "Michael", "initials": "M", "orcid": "0000-0002-2095-988X", "researcher": {"href": "https://publications.scilifelab.se/researcher/dbd70d20b66b4fb4a8d1308b2f54e2a2.json"}}, {"family": "Zwiewka", "given": "Marta", "initials": "M"}, {"family": "P\u011bn\u010d\u00edk", "given": "Ale\u0161", "initials": "A"}, {"family": "Almqvist", "given": "Fredrik", "initials": "F"}, {"family": "Ljung", "given": "Karin", "initials": "K", "orcid": "0000-0003-2901-189X", "researcher": {"href": "https://publications.scilifelab.se/researcher/f91b1e1f90c24559b915ebcd265804a4.json"}}, {"family": "Nov\u00e1k", "given": "Ond\u0159ej", "initials": "O", "orcid": "0000-0003-3452-0154", "researcher": {"href": "https://publications.scilifelab.se/researcher/8c19165acb9a4ff79dd96af7fccdc5f8.json"}}, {"family": "Robert", "given": "St\u00e9phanie", "initials": "S", "orcid": "0000-0002-0013-3239", "researcher": {"href": "https://publications.scilifelab.se/researcher/74a96c43520f4c9c91e44be8a7d39e6c.json"}}], "type": "journal article", "published": "2019-08-00", "journal": {"title": "New Phytol.", "issn": "1469-8137", "issn-l": "0028-646X", "volume": "223", "issue": "3", "pages": "1420-1432"}, "abstract": "distribution of auxin within plant tissues is of great importance for developmental plasticity, including root gravitropic growth. Auxin flow is directed by the subcellular polar distribution and dynamic relocalisation of auxin transporters such as the PIN-FORMED (PIN) efflux carriers, which can be influenced by the main natural plant auxin indole-3-acetic acid (IAA). Anthranilic acid (AA) is an important early precursor of IAA and previously published studies with AA analogues have suggested that AA may also regulate PIN localisation. Using Arabidopsis thaliana as a model species, we studied an AA-deficient mutant displaying agravitropic root growth, treated seedlings with AA and AA analogues and transformed lines to over-produce AA while inhibiting its conversion to downstream IAA precursors. We showed that AA rescues root gravitropic growth in the AA-deficient mutant at concentrations that do not rescue IAA levels. Overproduction of AA affects root gravitropism without affecting IAA levels. Treatments with, or deficiency in, AA result in defects in PIN polarity and gravistimulus-induced PIN relocalisation in root cells. Our results revealed a previously unknown role for AA in the regulation of PIN subcellular localisation and dynamics involved in root gravitropism, which is independent of its better known role in IAA biosynthesis.", "doi": "10.1111/nph.15877", "pmid": "31038751", "labels": {"Swedish Metabolomics Centre": "Service", "Chemical Biology Consortium Sweden": "Service"}, "xrefs": [], "notes": [], "created": "2020-01-07T15:52:13.037Z", "modified": "2025-10-17T13:04:28.531Z"}, {"entity": "publication", "iuid": "f59c91815d66466eba5b3ad85f9fe687", "links": {"self": {"href": "https://publications.scilifelab.se/publication/f59c91815d66466eba5b3ad85f9fe687.json"}, "display": {"href": "https://publications.scilifelab.se/publication/f59c91815d66466eba5b3ad85f9fe687"}}, "title": "Selective auxin agonists induce specific AUX/IAA protein degradation to modulate plant development.", "authors": [{"family": "Vain", "given": "Thomas", "initials": "T"}, {"family": "Raggi", "given": "Sara", "initials": "S", "orcid": "0000-0002-7925-5772", "researcher": {"href": "https://publications.scilifelab.se/researcher/b7f5c77f8e6e4904b5dd2d8241316589.json"}}, {"family": "Ferro", "given": "Noel", "initials": "N"}, {"family": "Barange", "given": "Deepak Kumar", "initials": "DK"}, {"family": "Kieffer", "given": "Martin", "initials": "M"}, {"family": "Ma", "given": "Qian", "initials": "Q"}, {"family": "Doyle", "given": "Siamsa M", "initials": "SM", "orcid": "0000-0003-4889-3496", "researcher": {"href": "https://publications.scilifelab.se/researcher/4a82fafd2ab34ff2bac338ce451bb4bb.json"}}, {"family": "Thelander", "given": "Mattias", "initials": "M"}, {"family": "Pa\u0159\u00edzkov\u00e1", "given": "Barbora", "initials": "B", "orcid": "0000-0002-8125-2271", "researcher": {"href": "https://publications.scilifelab.se/researcher/c9dd3d0c062245a5b188e774955cd210.json"}}, {"family": "Nov\u00e1k", "given": "Ond\u0159ej", "initials": "O", "orcid": "0000-0003-3452-0154", "researcher": {"href": "https://publications.scilifelab.se/researcher/8c19165acb9a4ff79dd96af7fccdc5f8.json"}}, {"family": "Ismail", "given": "Alexandre", "initials": "A"}, {"family": "Enquist", "given": "Per-Anders", "initials": "P"}, {"family": "Rigal", "given": "Adeline", "initials": "A"}, {"family": "\u0141angowska", "given": "Ma\u0142gorzata", "initials": "M"}, {"family": "Ramans Harborough", "given": "Sigurd", "initials": "S"}, {"family": "Zhang", "given": "Yi", "initials": "Y"}, {"family": "Ljung", "given": "Karin", "initials": "K", "orcid": "0000-0003-2901-189X", "researcher": {"href": "https://publications.scilifelab.se/researcher/f91b1e1f90c24559b915ebcd265804a4.json"}}, {"family": "Callis", "given": "Judy", "initials": "J"}, {"family": "Almqvist", "given": "Fredrik", "initials": "F", "orcid": "0000-0002-0504-4446", "researcher": {"href": "https://publications.scilifelab.se/researcher/6f8d848fccce44da9bcd4e79f7eae64c.json"}}, {"family": "Kepinski", "given": "Stefan", "initials": "S"}, {"family": "Estelle", "given": "Mark", "initials": "M"}, {"family": "Pauwels", "given": "Laurens", "initials": "L", "orcid": "0000-0002-0221-9052", "researcher": {"href": "https://publications.scilifelab.se/researcher/6cbb68c7a39a447d937693fede33a7c9.json"}}, {"family": "Robert", "given": "St\u00e9phanie", "initials": "S", "orcid": "0000-0002-0013-3239", "researcher": {"href": "https://publications.scilifelab.se/researcher/74a96c43520f4c9c91e44be8a7d39e6c.json"}}], "type": "journal article", "published": "2019-03-26", "journal": {"title": "Proc. Natl. Acad. Sci. U.S.A.", "issn": "1091-6490", "issn-l": "0027-8424", "volume": "116", "issue": "13", "pages": "6463-6472"}, "abstract": "Auxin phytohormones control most aspects of plant development through a complex and interconnected signaling network. In the presence of auxin, AUXIN/INDOLE-3-ACETIC ACID (AUX/IAA) transcriptional repressors are targeted for degradation by the SKP1-CULLIN1-F-BOX (SCF) ubiquitin-protein ligases containing TRANSPORT INHIBITOR RESISTANT 1/AUXIN SIGNALING F-BOX (TIR1/AFB). CULLIN1-neddylation is required for SCFTIR1/AFB functionality, as exemplified by mutants deficient in the NEDD8-activating enzyme subunit AUXIN-RESISTANT 1 (AXR1). Here, we report a chemical biology screen that identifies small molecules requiring AXR1 to modulate plant development. We selected four molecules of interest, RubNeddin 1 to 4 (RN1 to -4), among which RN3 and RN4 trigger selective auxin responses at transcriptional, biochemical, and morphological levels. This selective activity is explained by their ability to consistently promote the interaction between TIR1 and a specific subset of AUX/IAA proteins, stimulating the degradation of particular AUX/IAA combinations. Finally, we performed a genetic screen using RN4, the RN with the greatest potential for dissecting auxin perception, which revealed that the chromatin remodeling ATPase BRAHMA is implicated in auxin-mediated apical hook development. These results demonstrate the power of selective auxin agonists to dissect auxin perception for plant developmental functions, as well as offering opportunities to discover new molecular players involved in auxin responses.", "doi": "10.1073/pnas.1809037116", "pmid": "30850516", "labels": {"Swedish Metabolomics Centre": "Service", "Chemical Biology Consortium Sweden": "Collaborative"}, "xrefs": [{"db": "pii", "key": "1809037116"}, {"db": "pmc", "key": "PMC6442611"}], "notes": [], "created": "2019-06-17T08:28:28.156Z", "modified": "2025-10-17T13:04:28.584Z"}]}