{"entity": "researcher", "timestamp": "2026-08-11T14:43:35.770Z", "family": "Wang", "given": "Xiaoyang", "initials": "X", "orcid": "0000-0001-9717-8160", "affiliations": ["Centre of Perinatal Medicine and Health, Institute of Neuroscience and Physiology, Department of Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg 40530, Sweden.", "Centre of Perinatal Medicine and Health, Institute of Clinical Sciences, Department of Obstetrics and Gynecology, Sahlgrenska Academy, Gothenburg University, Gothenburg 40530, Sweden.", "Henan Key Laboratory of Child Brain Injury, Institute of Neuroscience, Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/70fbed19bca84bec93f558168444227a.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/70fbed19bca84bec93f558168444227a"}}, "publications": [{"entity": "publication", "iuid": "76a1e6398a4d4115b774554b4b61f807", "links": {"self": {"href": "https://publications.scilifelab.se/publication/76a1e6398a4d4115b774554b4b61f807.json"}, "display": {"href": "https://publications.scilifelab.se/publication/76a1e6398a4d4115b774554b4b61f807"}}, "title": "Transcriptome network analysis links perinatal Staphylococcus epidermidis infection to microglia reprogramming in the immature hippocampus.", "authors": [{"family": "Gravina", "given": "Giacomo", "initials": "G"}, {"family": "Ardalan", "given": "Maryam", "initials": "M"}, {"family": "Chumak", "given": "Tetyana", "initials": "T"}, {"family": "Rydbeck", "given": "Halfdan", "initials": "H"}, {"family": "Wang", "given": "Xiaoyang", "initials": "X", "orcid": "0000-0001-9717-8160", "researcher": {"href": "https://publications.scilifelab.se/researcher/70fbed19bca84bec93f558168444227a.json"}}, {"family": "Ek", "given": "Carl Joakim", "initials": "CJ"}, {"family": "Mallard", "given": "Carina", "initials": "C", "orcid": "0000-0001-8953-919X", "researcher": {"href": "https://publications.scilifelab.se/researcher/2d0fa7de10554b5bb5a0e1ee64902aa1.json"}}], "type": "journal article", "published": "2023-09-00", "journal": {"title": "Glia", "issn": "1098-1136", "volume": "71", "issue": "9", "pages": "2234-2249", "issn-l": "0894-1491"}, "abstract": "Staphylococcus epidermidis (S. epidermidis) is the most common nosocomial pathogen in preterm infants and associated with increased risk of cognitive delay, however, underlying mechanisms are unknown. We employed morphological, transcriptomic and physiological methods to extensively characterize microglia in the immature hippocampus following S. epidermidis infection. 3D morphological analysis revealed activation of microglia after S. epidermidis. Differential expression combined with network analysis identified NOD-receptor signaling and trans-endothelial leukocyte trafficking as major mechanisms in microglia. In support, active caspase-1 was increased in the hippocampus and using the LysM-eGFP knock-in transgenic mouse, we demonstrate infiltration of leukocytes to the brain together with disruption of the blood-brain barrier. Our findings identify activation of microglia inflammasome as a major mechanism underlying neuroinflammation following infection. The results demonstrate that neonatal S. epidermidis infection share analogies with S. aureus and neurological diseases, suggesting a previously unrecognized important role in neurodevelopmental disorders in preterm born children.", "doi": "10.1002/glia.24389", "pmid": "37246946", "labels": {"Clinical Genomics Gothenburg": "Service", "Clinical Genomics": "Service"}, "xrefs": [], "notes": [], "created": "2023-11-30T22:32:41.569Z", "modified": "2023-11-30T22:32:41.607Z"}, {"entity": "publication", "iuid": "ac80952db59f49f3b1c15eec06392e65", "links": {"self": {"href": "https://publications.scilifelab.se/publication/ac80952db59f49f3b1c15eec06392e65.json"}, "display": {"href": "https://publications.scilifelab.se/publication/ac80952db59f49f3b1c15eec06392e65"}}, "title": "Two different isoforms of osteopontin modulate myelination and axonal integrity.", "authors": [{"family": "Nilsson", "given": "Gisela", "initials": "G", "orcid": "0000-0003-4423-5082", "researcher": {"href": "https://publications.scilifelab.se/researcher/c07681f5131b4c58bd71119c56f5d435.json"}}, {"family": "Mottahedin", "given": "Amin", "initials": "A", "orcid": "0000-0002-3677-2198", "researcher": {"href": "https://publications.scilifelab.se/researcher/82a4c6f53ae241edaf5147addb082567.json"}}, {"family": "Zelco", "given": "Aura", "initials": "A", "orcid": "0000-0002-5851-6355", "researcher": {"href": "https://publications.scilifelab.se/researcher/f66e84b556ab4454b96ba929cfa2b104.json"}}, {"family": "Lauschke", "given": "Volker M", "initials": "VM", "orcid": "0000-0002-1140-6204", "researcher": {"href": "https://publications.scilifelab.se/researcher/29c123916fbf4948a911560c1a259496.json"}}, {"family": "Ek", "given": "C Joakim", "initials": "CJ", "orcid": "0000-0002-5764-7679", "researcher": {"href": "https://publications.scilifelab.se/researcher/991c705e88dd472cae9e98ad540660ab.json"}}, {"family": "Song", "given": "Juan", "initials": "J"}, {"family": "Ardalan", "given": "Maryam", "initials": "M", "orcid": "0000-0003-3414-1584", "researcher": {"href": "https://publications.scilifelab.se/researcher/ceef7775ba414a1ab0f5039db2f0014b.json"}}, {"family": "Hua", "given": "Sha", "initials": "S"}, {"family": "Zhang", "given": "Xiaoli", "initials": "X", "orcid": "0000-0001-5111-9405", "researcher": {"href": "https://publications.scilifelab.se/researcher/3d5f1af1b5f3452d8db56bbe3eea440c.json"}}, {"family": "Mallard", "given": "Carina", "initials": "C", "orcid": "0000-0001-8953-919X", "researcher": {"href": "https://publications.scilifelab.se/researcher/2d0fa7de10554b5bb5a0e1ee64902aa1.json"}}, {"family": "Hagberg", "given": "Henrik", "initials": "H", "orcid": "0000-0003-3962-1448", "researcher": {"href": "https://publications.scilifelab.se/researcher/ca1150b5f07c441096f2716c0564e1d0.json"}}, {"family": "Leavenworth", "given": "Jianmei W", "initials": "JW", "orcid": "0000-0002-4100-9883", "researcher": {"href": "https://publications.scilifelab.se/researcher/d1b287368adf4edabe671e33ab56c550.json"}}, {"family": "Wang", "given": "Xiaoyang", "initials": "X", "orcid": "0000-0001-9717-8160", "researcher": {"href": "https://publications.scilifelab.se/researcher/70fbed19bca84bec93f558168444227a.json"}}], "type": "journal article", "published": "2023-08-00", "journal": {"title": "FASEB Bioadv", "issn": "2573-9832", "issn-l": null, "volume": "5", "issue": "8", "pages": "336-353"}, "abstract": "Abnormal myelination underlies the pathology of white matter diseases such as preterm white matter injury and multiple sclerosis. Osteopontin (OPN) has been suggested to play a role in myelination. Murine OPN mRNA is translated into a secreted isoform (sOPN) or an intracellular isoform (iOPN). Whether there is an isoform-specific involvement of OPN in myelination is unknown. Here we generated mouse models that either lacked both OPN isoforms in all cells (OPN-KO) or lacked sOPN systemically but expressed iOPN specifically in oligodendrocytes (OLs-iOPN-KI). Transcriptome analysis of isolated oligodendrocytes from the neonatal brain showed that genes and pathways related to increase of myelination and altered cell cycle control were enriched in the absence of the two OPN isoforms in OPN-KO mice compared to control mice. Accordingly, adult OPN-KO mice showed an increased axonal myelination, as revealed by transmission electron microscopy imaging, and increased expression of myelin-related proteins. In contrast, neonatal oligodendrocytes from OLs-iOPN-KI mice compared to control mice showed differential regulation of genes and pathways related to the increase of cell adhesion, motility, and vasculature development, and the decrease of axonal/neuronal development. OLs-iOPN-KI mice showed abnormal myelin formation in the early phase of myelination in young mice and signs of axonal degeneration in adulthood. These results suggest an OPN isoform-specific involvement, and a possible interplay between the isoforms, in myelination, and axonal integrity. Thus, the two isoforms of OPN need to be separately considered in therapeutic strategies targeting OPN in white matter injury and diseases.", "doi": "10.1096/fba.2023-00030", "pmid": "37554545", "labels": {"Clinical Genomics Gothenburg": "Service", "Integrated Microscopy Technologies Gothenburg": "Service", "Clinical Genomics": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC10405251"}, {"db": "pii", "key": "FBA21405"}], "notes": [], "created": "2023-11-30T22:35:19.238Z", "modified": "2023-12-03T13:28:50.770Z"}, {"entity": "publication", "iuid": "d6f8fc37642e488783da206805ed9197", "links": {"self": {"href": "https://publications.scilifelab.se/publication/d6f8fc37642e488783da206805ed9197.json"}, "display": {"href": "https://publications.scilifelab.se/publication/d6f8fc37642e488783da206805ed9197"}}, "title": "Temporal brain transcriptome analysis reveals key pathological events after germinal matrix hemorrhage in neonatal rats.", "authors": [{"family": "Song", "given": "Juan", "initials": "J"}, {"family": "Nilsson", "given": "Gisela", "initials": "G"}, {"family": "Xu", "given": "Yiran", "initials": "Y"}, {"family": "Zelco", "given": "Aura", "initials": "A"}, {"family": "Rocha-Ferreira", "given": "Eridan", "initials": "E"}, {"family": "Wang", "given": "Yafeng", "initials": "Y"}, {"family": "Zhang", "given": "Xiaoli", "initials": "X"}, {"family": "Zhang", "given": "Shan", "initials": "S"}, {"family": "Ek", "given": "Joakim", "initials": "J"}, {"family": "Hagberg", "given": "Henrik", "initials": "H"}, {"family": "Zhu", "given": "Changlian", "initials": "C"}, {"family": "Wang", "given": "Xiaoyang", "initials": "X", "orcid": "0000-0001-9717-8160", "researcher": {"href": "https://publications.scilifelab.se/researcher/70fbed19bca84bec93f558168444227a.json"}}], "type": "journal article", "published": "2022-09-00", "journal": {"title": "J. Cereb. Blood Flow Metab.", "issn": "1559-7016", "volume": "42", "issue": "9", "pages": "1632-1649", "issn-l": "0271-678X"}, "abstract": "Germinal matrix hemorrhage (GMH) is a common complication in preterm infants and is associated with high risk of adverse neurodevelopmental outcomes. We used a rat GMH model and performed RNA sequencing to investigate the signaling pathways and biological processes following hemorrhage. GMH induced brain injury characterized by early hematoma and subsequent tissue loss. At 6 hours after GMH, gene expression indicated an increase in mitochondrial activity such as ATP metabolism and oxidative phosphorylation along with upregulation of cytoprotective pathways and heme metabolism. At 24 hours after GMH, the expression pattern suggested an increase in cell cycle progression and downregulation of neurodevelopmental-related pathways. At 72 hours after GMH, there was an increase in genes related to inflammation and an upregulation of ferroptosis. Hemoglobin components and genes related to heme metabolism and ferroptosis such as Hmox1, Alox15, and Alas2 were among the most upregulated genes. We observed dysregulation of processes involved in development, mitochondrial function, cholesterol biosynthesis, and inflammation, all of which contribute to neurodevelopmental deterioration following GMH. This study is the first temporal transcriptome profile providing a comprehensive overview of the molecular mechanisms underlying brain injury following GMH, and it provides useful guidance in the search for therapeutic interventions.", "doi": "10.1177/0271678X221098811", "pmid": "35491813", "labels": {"Integrated Microscopy Technologies Gothenburg": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC9441725"}], "notes": [], "created": "2023-02-16T08:25:37.304Z", "modified": "2023-02-16T08:25:37.329Z"}, {"entity": "publication", "iuid": "fd8ad9ec383e498c9f373930bccf2c76", "links": {"self": {"href": "https://publications.scilifelab.se/publication/fd8ad9ec383e498c9f373930bccf2c76.json"}, "display": {"href": "https://publications.scilifelab.se/publication/fd8ad9ec383e498c9f373930bccf2c76"}}, "title": "Single-cell atlas reveals meningeal leukocyte heterogeneity in the developing mouse brain.", "authors": [{"family": "Zelco", "given": "Aura", "initials": "A"}, {"family": "B\u00f6rjesson", "given": "Vanja", "initials": "V"}, {"family": "de Kanter", "given": "Jurrian K", "initials": "JK"}, {"family": "Lebrero-Fernandez", "given": "Cristina", "initials": "C"}, {"family": "Lauschke", "given": "Volker M", "initials": "VM"}, {"family": "Rocha-Ferreira", "given": "Eridan", "initials": "E"}, {"family": "Nilsson", "given": "Gisela", "initials": "G"}, {"family": "Nair", "given": "Syam", "initials": "S"}, {"family": "Svedin", "given": "Pernilla", "initials": "P"}, {"family": "Bemark", "given": "Mats", "initials": "M"}, {"family": "Hagberg", "given": "Henrik", "initials": "H"}, {"family": "Mallard", "given": "Carina", "initials": "C"}, {"family": "Holstege", "given": "Frank C P", "initials": "FCP"}, {"family": "Wang", "given": "Xiaoyang", "initials": "X", "orcid": "0000-0001-9717-8160", "researcher": {"href": "https://publications.scilifelab.se/researcher/70fbed19bca84bec93f558168444227a.json"}}], "type": "journal article", "published": "2021-08-01", "journal": {"title": "Genes Dev.", "issn": "1549-5477", "issn-l": "0890-9369", "volume": "35", "issue": "15-16", "pages": "1190-1207"}, "abstract": "The meninges are important for brain development and pathology. Using single-cell RNA sequencing, we have generated the first comprehensive transcriptional atlas of neonatal mouse meningeal leukocytes under normal conditions and after perinatal brain injury. We identified almost all known leukocyte subtypes and found differences between neonatal and adult border-associated macrophages, thus highlighting that neonatal border-associated macrophages are functionally immature with regards to immune responses compared with their adult counterparts. We also identified novel meningeal microglia-like cell populations that may participate in white matter development. Early after the hypoxic-ischemic insult, neutrophil numbers increased and they exhibited increased granulopoiesis, suggesting that the meninges are an important site of immune cell expansion with implications for the initiation of inflammatory cascades after neonatal brain injury. Our study provides a single-cell resolution view of the importance of meningeal leukocytes at the early stage of development in health and disease.", "doi": "10.1101/gad.348190.120", "pmid": "34301765", "labels": {"Clinical Genomics Gothenburg": "Collaborative", "Clinical Genomics": "Collaborative"}, "xrefs": [{"db": "pii", "key": "gad.348190.120"}, {"db": "pmc", "key": "PMC8336895"}], "notes": [], "created": "2022-03-29T13:44:08.378Z", "modified": "2023-05-15T16:44:24.926Z"}]}