{"entity": "researcher", "timestamp": "2026-06-15T18:03:28.751Z", "family": "Dolan", "given": "Brendan", "initials": "B", "orcid": "0000-0001-9062-3882", "affiliations": ["Department of Medical Biochemistry and Cell Biology, University of Gothenburg, 405 30 Gothenburg, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/6ff7d8f515ef4707ab6bca8da108f115.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/6ff7d8f515ef4707ab6bca8da108f115"}}, "publications": [{"entity": "publication", "iuid": "313987203417421e9c853c7e0a9afc12", "links": {"self": {"href": "https://publications.scilifelab.se/publication/313987203417421e9c853c7e0a9afc12.json"}, "display": {"href": "https://publications.scilifelab.se/publication/313987203417421e9c853c7e0a9afc12"}}, "title": "MUC17 is an essential small intestinal glycocalyx component that is disrupted in Crohn's disease.", "authors": [{"family": "Layunta", "given": "Elena", "initials": "E"}, {"family": "J\u00e4verfelt", "given": "Sofia", "initials": "S"}, {"family": "van de Koolwijk", "given": "Fleur C", "initials": "FC"}, {"family": "Sivertsson", "given": "Molly", "initials": "M"}, {"family": "Dolan", "given": "Brendan", "initials": "B", "orcid": "0000-0001-9062-3882", "researcher": {"href": "https://publications.scilifelab.se/researcher/6ff7d8f515ef4707ab6bca8da108f115.json"}}, {"family": "Arike", "given": "Liisa", "initials": "L", "orcid": "0000-0002-2184-0960", "researcher": {"href": "https://publications.scilifelab.se/researcher/8a6ecb751dca49a598e3d13cc92ee5cf.json"}}, {"family": "Thulin", "given": "Sara Im", "initials": "SI"}, {"family": "Vallance", "given": "Bruce A", "initials": "BA"}, {"family": "Pelaseyed", "given": "Thaher", "initials": "T", "orcid": "0000-0002-6434-3913", "researcher": {"href": "https://publications.scilifelab.se/researcher/9dc0aa3d9762420caa7efaaa19c1174b.json"}}], "type": "journal article", "published": "2024-12-19", "journal": {"title": "JCI Insight", "issn": "2379-3708", "volume": "10", "issue": "3", "issn-l": "2379-3708"}, "abstract": "Crohn's disease (CD) is the chronic inflammation of the terminal ileum and colon triggered by a dysregulated immune response to bacteria, but insights into specific molecular perturbations at the critical bacteria-epithelium interface are limited. Here, we report that the membrane mucin MUC17 protected small intestinal enterocytes against commensal and pathogenic bacteria. In noninflamed CD ileum, reduced MUC17 levels and a compromised glycocalyx barrier allowed recurrent bacterial contact with enterocytes. Muc17 deletion in mice rendered the small intestine particularly prone to atypical bacterial infection while maintaining resistance to colitis. The loss of Muc17 resulted in spontaneous deterioration of epithelial homeostasis and in the extraintestinal translocation of bacteria. Finally, Muc17-deficient mice harbored specific small intestinal bacterial taxa observed in patients with CD. Our findings highlight MUC17 as an essential region-specific line of defense in the small intestine with relevance for early epithelial defects in CD.", "doi": "10.1172/jci.insight.181481", "pmid": "39699961", "labels": {"Bioinformatics Support for Computational Resources": "Service", "Glycoproteomics and MS Proteomics": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC11948581"}, {"db": "pii", "key": "181481"}], "notes": [], "created": "2025-02-28T14:21:40.060Z", "modified": "2025-11-28T10:52:24.618Z"}, {"entity": "publication", "iuid": "40fe795e4a6b41dc96ed06c6d7accad8", "links": {"self": {"href": "https://publications.scilifelab.se/publication/40fe795e4a6b41dc96ed06c6d7accad8.json"}, "display": {"href": "https://publications.scilifelab.se/publication/40fe795e4a6b41dc96ed06c6d7accad8"}}, "title": "BPP43_05035 is a Brachyspira pilosicoli cell surface adhesin that weakens the integrity of the epithelial barrier during infection.", "authors": [{"family": "Rajan", "given": "Anandi", "initials": "A", "orcid": "0000-0002-4873-8528", "researcher": {"href": "https://publications.scilifelab.se/researcher/261182551e72486895b04cebcaa22d69.json"}}, {"family": "Gallego", "given": "Pablo", "initials": "P"}, {"family": "Dolan", "given": "Brendan", "initials": "B", "orcid": "0000-0001-9062-3882", "researcher": {"href": "https://publications.scilifelab.se/researcher/6ff7d8f515ef4707ab6bca8da108f115.json"}}, {"family": "Patel", "given": "Piyush", "initials": "P"}, {"family": "Dwibedi", "given": "Chinmay", "initials": "C", "orcid": "0000-0001-6416-4440", "researcher": {"href": "https://publications.scilifelab.se/researcher/a0b0dbf807be449da1856c0c018f13a3.json"}}, {"family": "Luis", "given": "Ana S", "initials": "AS", "orcid": "0000-0002-5086-7353", "researcher": {"href": "https://publications.scilifelab.se/researcher/06abcf6fc3584357afbd80d6537fdd48.json"}}, {"family": "Trillo-Muyo", "given": "Sergio", "initials": "S", "orcid": "0000-0002-3135-9134", "researcher": {"href": "https://publications.scilifelab.se/researcher/2e6b6b830e9145a2ae3e6c10895acbee.json"}}, {"family": "Arike", "given": "Liisa", "initials": "L", "orcid": "0000-0002-2184-0960", "researcher": {"href": "https://publications.scilifelab.se/researcher/8a6ecb751dca49a598e3d13cc92ee5cf.json"}}, {"family": "van der Post", "given": "Sjoerd", "initials": "S", "orcid": "0000-0002-7965-5311", "researcher": {"href": "https://publications.scilifelab.se/researcher/26adc68875cb4cb08bcf969868b42890.json"}}, {"family": "Simr\u00e9n", "given": "Magnus", "initials": "M", "orcid": "0000-0003-1931-4926", "researcher": {"href": "https://publications.scilifelab.se/researcher/bef16e586fb144c4897df021225f3843.json"}}, {"family": "Pelaseyed", "given": "Thaher", "initials": "T", "orcid": "0000-0002-6434-3913", "researcher": {"href": "https://publications.scilifelab.se/researcher/9dc0aa3d9762420caa7efaaa19c1174b.json"}}], "type": "journal article", "published": "2024-09-30", "journal": {"title": "Gut Microbes", "issn": "1949-0984", "volume": "16", "issue": "1", "pages": "2409247", "issn-l": null}, "abstract": "The anaerobic spirochete Brachyspira causes intestinal spirochetosis, characterized by the intimate attachment of bacterial cells to the colonic mucosa, potentially leading to symptoms such as diarrhea, abdominal pain, and weight loss. Despite the clinical significance of Brachyspira infections, the mechanism of the interaction between Brachyspira and the colon epithelium is not known. We characterized the molecular mechanism of the B. pilosicoli-epithelium interaction and its impact on the epithelial barrier during infection. Through a proteomics approach, we identified BPP43_05035 as a candidate B. pilosicoli surface protein that mediates bacterial attachment to cultured human colonic epithelial cells. The crystal structure of BPP43_05035 revealed a globular lipoprotein with a six-bladed beta-propeller domain. Blocking the native BPP43_05035 on B. pilosicoli, either with a specific antibody or via competitive inhibition, abrogated its binding to epithelial cells, which required cell surface-exposed N-glycans. Proximity labeling and interaction assays revealed that BPP43_05035 bound to tight junctions, thereby increasing the permeability of the epithelial monolayer. Extending our investigation to humans, we discovered a downregulation of tight junction and brush border genes in B. pilosicoli-infected patients carrying detectable levels of epithelium-bound BPP43_05035. Collectively, our findings identify BPP43_05035 as a B. pilosicoli adhesin that weakens the colonic epithelial barrier during infection.", "doi": "10.1080/19490976.2024.2409247", "pmid": "39349383", "labels": {"Glycoproteomics and MS Proteomics": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC11444514"}], "notes": [], "created": "2024-11-27T15:36:03.615Z", "modified": "2024-11-27T15:36:03.880Z"}, {"entity": "publication", "iuid": "d4607c3b5d1647179a3ed651e6923fa2", "links": {"self": {"href": "https://publications.scilifelab.se/publication/d4607c3b5d1647179a3ed651e6923fa2.json"}, "display": {"href": "https://publications.scilifelab.se/publication/d4607c3b5d1647179a3ed651e6923fa2"}}, "title": "Clearance of small intestinal crypts involves goblet cell mucus secretion by intracellular granule rupture and enterocyte ion transport.", "authors": [{"family": "Dolan", "given": "Brendan", "initials": "B", "orcid": "0000-0001-9062-3882", "researcher": {"href": "https://publications.scilifelab.se/researcher/6ff7d8f515ef4707ab6bca8da108f115.json"}}, {"family": "Ermund", "given": "Anna", "initials": "A", "orcid": "0000-0002-3233-043X", "researcher": {"href": "https://publications.scilifelab.se/researcher/8215eeb434f84deaafa2f9f198bfb2bc.json"}}, {"family": "Martinez-Abad", "given": "Beatriz", "initials": "B", "orcid": "0000-0002-0521-3473", "researcher": {"href": "https://publications.scilifelab.se/researcher/2d3781ca1b0245908c2030b4707cc34d.json"}}, {"family": "Johansson", "given": "Malin E V", "initials": "MEV", "orcid": "0000-0002-4237-6677", "researcher": {"href": "https://publications.scilifelab.se/researcher/520dab35c19049c8b3f1083a92e60d56.json"}}, {"family": "Hansson", "given": "Gunnar C", "initials": "GC", "orcid": "0000-0002-1900-1869", "researcher": {"href": "https://publications.scilifelab.se/researcher/44b3815603154322a6dac16f2fc1c1e9.json"}}], "type": "journal article", "published": "2022-09-20", "journal": {"title": "Sci Signal", "issn": "1937-9145", "volume": "15", "issue": "752", "pages": "eabl5848", "issn-l": "1945-0877"}, "abstract": "Goblet cells in the small intestinal crypts contain large numbers of mucin granules that are rapidly discharged to clean bacteria from the crypt. Because acetylcholine released by neuronal and nonneuronal cells controls many aspects of intestinal epithelial function, we used tissue explants and organoids to investigate the response of the small intestinal crypt to cholinergic stimulation. The activation of muscarinic acetylcholine receptors initiated a coordinated and rapid emptying of crypt goblet cells that flushed the crypt contents into the intestinal lumen. Cholinergic stimulation induced an expansion of the granule contents followed by intracellular rupture of the mucin granules. The mucus expanded intracellularly before the rupture of the goblet cell apical membrane and continued to expand after its release into the crypt lumen. The goblet cells recovered from membrane rupture and replenished their stores of mucin granules. Mucus secretion from the goblet cells depended on Ca2+ signaling and the expansion of the mucus in the crypt depended on gap junctions and on ion and water transport by enterocytes adjacent to the goblet cells. This distinctive mode of mucus secretion, which we refer to as \"expanding secretion,\" efficiently cleans the small intestine crypt through coordinated mucus, ion, and fluid secretion by goblet cells and enterocytes.", "doi": "10.1126/scisignal.abl5848", "pmid": "36126118", "labels": {"Integrated Microscopy Technologies Gothenburg": "Service"}, "xrefs": [{"db": "mid", "key": "NIHMS1851593"}, {"db": "pmc", "key": "PMC9749883"}], "notes": [], "created": "2023-02-16T08:26:10.686Z", "modified": "2023-02-16T08:26:10.773Z"}]}