{"entity": "researcher", "timestamp": "2026-08-19T20:37:41.346Z", "family": "Nowakowska", "given": "Maria B", "initials": "MB", "orcid": "0000-0003-2592-2442", "affiliations": ["Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/6d7fb5e257e941fd8738d9ff147d6b61.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/6d7fb5e257e941fd8738d9ff147d6b61"}}, "publications": [{"entity": "publication", "iuid": "588352e9404f4729b0d75cdc614fd16e", "links": {"self": {"href": "https://publications.scilifelab.se/publication/588352e9404f4729b0d75cdc614fd16e.json"}, "display": {"href": "https://publications.scilifelab.se/publication/588352e9404f4729b0d75cdc614fd16e"}}, "title": "Structure of the complete 14-subunit botulinum neurotoxin B complex reveals a unique anchoring through the narrow central pore of HA70.", "authors": [{"family": "Kr\u010d", "given": "Ajda", "initials": "A", "orcid": "0009-0007-5842-8527", "researcher": {"href": "https://publications.scilifelab.se/researcher/cda5c360c20149279692c5d69df7a694.json"}}, {"family": "Ko\u0161enina", "given": "Sara Persson", "initials": "SP", "orcid": "0000-0001-7893-0249", "researcher": {"href": "https://publications.scilifelab.se/researcher/9370d4ecf19c438bb205c43c23f94f26.json"}}, {"family": "Nowakowska", "given": "Maria B", "initials": "MB", "orcid": "0000-0003-2592-2442", "researcher": {"href": "https://publications.scilifelab.se/researcher/6d7fb5e257e941fd8738d9ff147d6b61.json"}}, {"family": "Masuyer", "given": "Geoffrey", "initials": "G", "orcid": "0000-0002-9527-2310", "researcher": {"href": "https://publications.scilifelab.se/researcher/41dcc0806dba4a56bb04725812f3a000.json"}}, {"family": "Stenmark", "given": "P\u00e5l", "initials": "P", "orcid": "0000-0003-4777-3417", "researcher": {"href": "https://publications.scilifelab.se/researcher/d97eba9f5edf4d76a5259c4baa8366c5.json"}}], "type": "journal article", "published": "2025-08-29", "journal": {"title": "Sci Adv", "issn": "2375-2548", "volume": "11", "issue": "35", "pages": "eadx5058", "issn-l": "2375-2548"}, "abstract": "Botulinum neurotoxin serotype B1 (BoNT/B) is a highly potent neurotoxin and therapeutic agent. Here, we present the structure of the complete 14-subunit (780 kDa) progenitor toxin complex (L-PTC) and of five subcomplexes. The structures show how the toxin interacts with its associated components in their role to protect and deliver BoNT/B across epithelial barriers. Each subcomplex, including the M-PTC, M-PTC-HA70, NTNH-HA70, and HA70 trimer, provides detailed understanding of the assembly mechanism, in which the NTNH-nLoop adopts a unique fold that locks the M-PTC into a central pore formed by HA70. The HA subcomplex presents a tripod architecture with flexible legs that may adapt to the rugged cell surface. Mass photometry reveals the pH dependence of BoNT/B release from the complex which is unexpectedly influenced by the presence of HA70. This study provides the complete L-PTC structure, offering insights into its assemblage and supporting the development of countermeasures and therapeutic applications.", "doi": "10.1126/sciadv.adx5058", "pmid": "40864696", "labels": {"Cryo-EM": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC12383256"}], "notes": [], "created": "2025-09-03T12:07:51.156Z", "modified": "2025-11-24T10:54:02.699Z"}]}