{"entity": "researcher", "timestamp": "2026-07-20T13:15:36.278Z", "family": "Birgersson", "given": "Madeleine", "initials": "M", "orcid": "0000-0002-5876-0710", "affiliations": ["Science for Life Laboratory, Department of Protein Science, KTH Royal Institute of Technology, 171 21 Solna, Sweden.", "Department of Biosciences and Nutrition, Karolinska Institutet, 141 83 Huddinge, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/68ea3a27e23a4f978e9c4e74ebdfbf11.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/68ea3a27e23a4f978e9c4e74ebdfbf11"}}, "publications": [{"entity": "publication", "iuid": "92139d604e30428c85b0f16ddccb99c8", "links": {"self": {"href": "https://publications.scilifelab.se/publication/92139d604e30428c85b0f16ddccb99c8.json"}, "display": {"href": "https://publications.scilifelab.se/publication/92139d604e30428c85b0f16ddccb99c8"}}, "title": "Spatial profiling of the mouse colonic immune landscape associated with colitis and sex.", "authors": [{"family": "Holm", "given": "Matilda", "initials": "M"}, {"family": "Stepanauskait\u0117", "given": "Lina", "initials": "L", "orcid": "0000-0003-4173-6009", "researcher": {"href": "https://publications.scilifelab.se/researcher/429f33fed9a44ff4b70ff88d5af917d9.json"}}, {"family": "B\u00e4ckstr\u00f6m", "given": "Anna", "initials": "A"}, {"family": "Birgersson", "given": "Madeleine", "initials": "M", "orcid": "0000-0002-5876-0710", "researcher": {"href": "https://publications.scilifelab.se/researcher/68ea3a27e23a4f978e9c4e74ebdfbf11.json"}}, {"family": "Socciarelli", "given": "Fabio", "initials": "F"}, {"family": "Archer", "given": "Amena", "initials": "A", "orcid": "0000-0002-0400-4151", "researcher": {"href": "https://publications.scilifelab.se/researcher/4502538fe3e84cb6a6618c972fa10b08.json"}}, {"family": "Stadler", "given": "Charlotte", "initials": "C", "orcid": "0000-0002-6781-1938", "researcher": {"href": "https://publications.scilifelab.se/researcher/2db3b27c7d7143cbacc8c1dd8ac90a31.json"}}, {"family": "Williams", "given": "Cecilia", "initials": "C", "orcid": "0000-0002-0602-2062", "researcher": {"href": "https://publications.scilifelab.se/researcher/89989da8d4e64dd3a30b87fc62ceefae.json"}}], "type": "journal article", "published": "2024-11-29", "journal": {"title": "Commun Biol", "issn": "2399-3642", "issn-l": "2399-3642", "volume": "7", "issue": "1", "pages": "1595"}, "abstract": "Inflammatory intestinal conditions are a major disease burden. Numerous factors shape the distribution of immune cells in the colon, but a spatial characterization of the homeostatic and inflamed colonic immune microenvironment is lacking. Here, we use the COMET platform for multiplex immunofluorescence to profile the infiltration of nine immune cell populations in mice of both sexes (N = 16) with full spatial context, including in regions of squamous metaplasia. Unsupervised clustering, neighborhood analysis, and manual quantification along the proximal-distal axis characterized the colonic immune landscape, quantified cell-cell interactions, and revealed sex differences. The distal colon was the most affected region during colitis, which was pronounced in males, who exhibited a sex-dependent increase of B cells and reduction of M2-like macrophages. Regions of squamous metaplasia exhibited strong infiltration of numerous immune cell populations, especially in males. Females exhibited more helper T cells and neutrophils at homeostasis and increased M2-like macrophage infiltration in the mid-colon upon colitis. Sex differences were corroborated by plasma cytokine profiles. Our results provide a foundation for future studies of inflammatory intestinal conditions.", "doi": "10.1038/s42003-024-07276-1", "pmid": "39613949", "labels": {"Affinity Proteomics Stockholm": "Service", "Spatial Proteomics": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC11606951"}, {"db": "pii", "key": "10.1038/s42003-024-07276-1"}], "notes": [], "created": "2024-12-04T20:14:13.972Z", "modified": "2025-02-17T09:58:33.843Z"}, {"entity": "publication", "iuid": "923e09636e01418bba0d80b842c05813", "links": {"self": {"href": "https://publications.scilifelab.se/publication/923e09636e01418bba0d80b842c05813.json"}, "display": {"href": "https://publications.scilifelab.se/publication/923e09636e01418bba0d80b842c05813"}}, "title": "High-fat diet and estrogen modulate the gut microbiota in a sex-dependent manner in mice.", "authors": [{"family": "Hases", "given": "Linnea", "initials": "L", "orcid": "0000-0001-6741-7204", "researcher": {"href": "https://publications.scilifelab.se/researcher/eeb5e09b88e94ab59a6ca9f8c457c5f4.json"}}, {"family": "Stepanauskaite", "given": "Lina", "initials": "L", "orcid": "0000-0003-4173-6009", "researcher": {"href": "https://publications.scilifelab.se/researcher/429f33fed9a44ff4b70ff88d5af917d9.json"}}, {"family": "Birgersson", "given": "Madeleine", "initials": "M", "orcid": "0000-0002-5876-0710", "researcher": {"href": "https://publications.scilifelab.se/researcher/68ea3a27e23a4f978e9c4e74ebdfbf11.json"}}, {"family": "Brusselaers", "given": "Nele", "initials": "N"}, {"family": "Schuppe-Koistinen", "given": "Ina", "initials": "I"}, {"family": "Archer", "given": "Amena", "initials": "A", "orcid": "0000-0002-0400-4151", "researcher": {"href": "https://publications.scilifelab.se/researcher/4502538fe3e84cb6a6618c972fa10b08.json"}}, {"family": "Engstrand", "given": "Lars", "initials": "L"}, {"family": "Williams", "given": "Cecilia", "initials": "C", "orcid": "0000-0002-0602-2062", "researcher": {"href": "https://publications.scilifelab.se/researcher/89989da8d4e64dd3a30b87fc62ceefae.json"}}], "type": "journal article", "published": "2023-01-09", "journal": {"title": "Commun Biol", "issn": "2399-3642", "volume": "6", "issue": "1", "pages": "20", "issn-l": "2399-3642"}, "abstract": "A high-fat diet can lead to gut microbiota dysbiosis, chronic intestinal inflammation, and metabolic syndrome. Notably, resulting phenotypes, such as glucose and insulin levels, colonic crypt cell proliferation, and macrophage infiltration, exhibit sex differences, and females are less affected. This is, in part, attributed to sex hormones. To investigate if there are sex differences in the microbiota and if estrogenic ligands can attenuate high-fat diet-induced dysbiosis, we used whole-genome shotgun sequencing to characterize the impact of diet, sex, and estrogenic ligands on the microbial composition of the cecal content of mice. We here report clear host sex differences along with remarkably sex-dependent responses to high-fat diet. Females, specifically, exhibited increased abundance of Blautia hansenii, and its levels correlated negatively with insulin levels in both sexes. Estrogen treatment had a modest impact on the microbiota diversity but altered a few important species in males. This included Collinsella aerofaciens F, which we show correlated with colonic macrophage infiltration. In conclusion, male and female mice exhibit clear differences in their cecal microbial composition and in how diet and estrogens impact the composition. Further, specific microbial strains are significantly correlated with metabolic parameters.", "doi": "10.1038/s42003-022-04406-5", "pmid": "36624306", "labels": {"Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC9829864"}, {"db": "pii", "key": "10.1038/s42003-022-04406-5"}], "notes": [], "created": "2023-11-27T21:49:55.417Z", "modified": "2024-01-16T13:48:34.180Z"}, {"entity": "publication", "iuid": "df3618d6438546bdbd1578a94586afc8", "links": {"self": {"href": "https://publications.scilifelab.se/publication/df3618d6438546bdbd1578a94586afc8.json"}, "display": {"href": "https://publications.scilifelab.se/publication/df3618d6438546bdbd1578a94586afc8"}}, "title": "Colitis Induces Sex-Specific Intestinal Transcriptomic Responses in Mice.", "authors": [{"family": "Hases", "given": "Linnea", "initials": "L"}, {"family": "Birgersson", "given": "Madeleine", "initials": "M", "orcid": "0000-0002-5876-0710", "researcher": {"href": "https://publications.scilifelab.se/researcher/68ea3a27e23a4f978e9c4e74ebdfbf11.json"}}, {"family": "Indukuri", "given": "Rajitha", "initials": "R", "orcid": "0000-0001-6570-842X", "researcher": {"href": "https://publications.scilifelab.se/researcher/94148ec0ffb74f9bb0243c18a1256c22.json"}}, {"family": "Archer", "given": "Amena", "initials": "A", "orcid": "0000-0002-0400-4151", "researcher": {"href": "https://publications.scilifelab.se/researcher/4502538fe3e84cb6a6618c972fa10b08.json"}}, {"family": "Williams", "given": "Cecilia", "initials": "C", "orcid": "0000-0002-0602-2062", "researcher": {"href": "https://publications.scilifelab.se/researcher/89989da8d4e64dd3a30b87fc62ceefae.json"}}], "type": "journal article", "published": "2022-09-08", "journal": {"title": "Int J Mol Sci", "issn": "1422-0067", "issn-l": null, "volume": "23", "issue": "18", "pages": null}, "abstract": "There are significant sex differences in colorectal cancer (CRC), including in incidence, onset, and molecular characteristics. Further, while inflammatory bowel disease (IBD) is a risk factor for CRC in both sexes, men with IBD have a 60% higher risk of developing CRC compared to women. In this study, we investigated sex differences during colitis-associated CRC (CAC) using a chemically induced CAC mouse model. The mice were treated with azoxymethane (AOM) and dextran sodium sulfate (DSS) and followed for 9 and 15 weeks. We performed RNA-sequencing of colon samples from males (n = 15) and females (n = 15) to study different stages of inflammation and identify corresponding transcriptomic sex differences in non-tumor colon tissue. We found a significant transcriptome response to AOM/DSS treatment in both sexes, including in pathways related to inflammation and cell proliferation. Notably, we found a stronger response in males and that male-specific differentially expressed genes were involved in NF\u03baB signaling and circadian rhythm. Further, an overrepresented proportion of male-specific gene regulations were predicted to be targets of Stat3, whereas for females, targets of the glucocorticoid receptor (Gr/Nr3c1) were overrepresented. At 15 weeks, the most apparent sex difference involved genes with functions in T cell proliferation, followed by the regulation of demethylases. The majority of sex differences were thus related to inflammation and the immune system. Our novel data, profiling the transcriptomic response to chemically induced colitis and CAC, indicate clear sex differences in CRC initiation and progression.", "doi": "10.3390/ijms231810408", "pmid": "36142324", "labels": {"National Genomics Infrastructure": "Service", "NGI Stockholm (Genomics Production)": "Service", "NGI Short read": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC9499483"}, {"db": "pii", "key": "ijms231810408"}], "notes": [], "created": "2022-12-19T10:36:01.618Z", "modified": "2023-10-16T12:40:09.780Z"}]}