{"entity": "researcher", "timestamp": "2026-03-17T03:09:16.519Z", "family": "Oltean", "given": "Mihai", "initials": "M", "orcid": "0000-0003-3783-5207", "affiliations": ["The Transplant Institute, Sahlgrenska University Hospital, 41345 Gothenburg, Sweden.", "Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy at the University of Gothenburg, 41345 Gothenburg, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/60a46e232d2644afbe9a4f3d89316a7a.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/60a46e232d2644afbe9a4f3d89316a7a"}}, "publications": [{"entity": "publication", "iuid": "fa1f2b26ed3949e2bdbd3c28dd0edf08", "links": {"self": {"href": "https://publications.scilifelab.se/publication/fa1f2b26ed3949e2bdbd3c28dd0edf08.json"}, "display": {"href": "https://publications.scilifelab.se/publication/fa1f2b26ed3949e2bdbd3c28dd0edf08"}}, "title": "Cryo-EM structure of CLCA1 identifies CLCA1 as a founding member of a novel metzincin family", "authors": [{"family": "Nystr\u00f6m", "given": "Elisabeth", "initials": "E", "orcid": "0000-0002-6970-7894", "researcher": {"href": "https://publications.scilifelab.se/researcher/09bd302f8a1341f6a1a5aaf3bfe94a94.json"}}, {"family": "van der Post", "given": "Sjoerd", "initials": "S", "orcid": "0000-0002-7965-5311", "researcher": {"href": "https://publications.scilifelab.se/researcher/26adc68875cb4cb08bcf969868b42890.json"}}, {"family": "Barrett", "given": "Doireann Bradley", "initials": "DB"}, {"family": "Raba", "given": "Grete", "initials": "G", "orcid": "0000-0002-7764-3878", "researcher": {"href": "https://publications.scilifelab.se/researcher/e9c5c71873aa4992bec09f9f6aabba5c.json"}}, {"family": "Pelaseyed", "given": "Thaher", "initials": "T", "orcid": "0000-0002-6434-3913", "researcher": {"href": "https://publications.scilifelab.se/researcher/9dc0aa3d9762420caa7efaaa19c1174b.json"}}, {"family": "Oltean", "given": "Mihai", "initials": "M", "orcid": "0000-0003-3783-5207", "researcher": {"href": "https://publications.scilifelab.se/researcher/60a46e232d2644afbe9a4f3d89316a7a.json"}}, {"family": "Luis", "given": "Ana S", "initials": "AS", "orcid": "0000-0002-5086-7353", "researcher": {"href": "https://publications.scilifelab.se/researcher/06abcf6fc3584357afbd80d6537fdd48.json"}}, {"family": "Trillo-Muyo", "given": "Sergio", "initials": "S", "orcid": "0000-0002-3135-9134", "researcher": {"href": "https://publications.scilifelab.se/researcher/2e6b6b830e9145a2ae3e6c10895acbee.json"}}], "type": "posted-content", "published": "2025-10-18", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2025.10.18.683246", "pmid": null, "labels": {"Integrated Microscopy Technologies Gothenburg": "Service"}, "xrefs": [], "notes": [], "created": "2026-01-12T10:07:24.335Z", "modified": "2026-01-12T10:07:24.558Z"}, {"entity": "publication", "iuid": "6c6cbdbfe7064dcfb2e87e001b74625e", "links": {"self": {"href": "https://publications.scilifelab.se/publication/6c6cbdbfe7064dcfb2e87e001b74625e.json"}, "display": {"href": "https://publications.scilifelab.se/publication/6c6cbdbfe7064dcfb2e87e001b74625e"}}, "title": "Proteomic analysis of human kidney biopsies unveils emerging acute kidney injury very early after liver graft reperfusion.", "authors": [{"family": "Nor\u00e9n", "given": "\u00c5sa", "initials": "\u00c5"}, {"family": "Boi", "given": "Roberto", "initials": "R"}, {"family": "Pullerits", "given": "Rille", "initials": "R"}, {"family": "M\u00f6lne", "given": "Johan", "initials": "J"}, {"family": "Ebefors", "given": "Kerstin", "initials": "K"}, {"family": "Friman", "given": "Styrbj\u00f6rn", "initials": "S"}, {"family": "Sihlbom", "given": "Carina", "initials": "C"}, {"family": "Herlenius", "given": "Gustaf", "initials": "G"}, {"family": "Nystr\u00f6m", "given": "Jenny", "initials": "J"}, {"family": "Oltean", "given": "Mihai", "initials": "M", "orcid": "0000-0003-3783-5207", "researcher": {"href": "https://publications.scilifelab.se/researcher/60a46e232d2644afbe9a4f3d89316a7a.json"}}], "type": "journal article", "published": "2025-06-16", "journal": {"title": "J Transl Med", "issn": "1479-5876", "volume": "23", "issue": "1", "pages": "658", "issn-l": "1479-5876"}, "abstract": "Acute kidney injury (AKI) is a frequent complication after liver transplantation (LT) and is associated with morbidity, mortality, and late development of chronic kidney disease. Risk factors for AKI after LT include patient, perioperative and graft-related factors. The exact renal molecular mechanisms behind AKI in LT are unclear.\n\nAlterations in the proteome were investigated in kidney biopsies from 21 patients undergoing LT using quantitative proteomics. The most upregulated protein was validated using immunohistochemistry. In addition, serum levels of interleukin (IL)-33, insulin-like growth factor binding protein (IGFBP)-7 and high-mobility group box (HMGB)-1 were analyzed. In silico data validation was performed using 14 recently published proteomics and transcriptomics datasets.\n\nIn post-reperfusion biopsies, we identified 731 differentially regulated proteins between patients with and without AKI. The most upregulated pathways were related to inflammation, integrin signaling and extracellular matrix (ECM) remodeling. The most downregulated pathways were traceable to a mitochondrial origin. HMGB-1 was found to be already upregulated (15%) 2 h after LT in patients who later developed AKI. The AKI group also showed upregulation of the alarmin IGFBP-7, caspases 1, 4 and 8, nuclear factor kappa B subunits, and the inflammasome adaptor protein PYCARD. Circulating IL-33 and HMGB-1 (but not IGFBP-7) increased during LT but returned to normal levels within 24 h. Altogether, these findings indicate ongoing inflammatory signaling activity in the kidneys of LT recipients who ultimately develop moderate or severe AKI shortly after liver graft reperfusion.\n\nLT induces extensive alarmin signaling and ECM remodeling in the kidneys of recipients who develop postoperative AKI. Further strategies to curtail this phenomenon are mandated. Trial registration https://www.researchweb.org/is/en/vgr/project/278585 , Registered 24 May 2022 (Retrospectively registered).", "doi": "10.1186/s12967-025-06695-w", "pmid": "40524147", "labels": {"Glycoproteomics and MS Proteomics": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC12172208"}, {"db": "pii", "key": "10.1186/s12967-025-06695-w"}], "notes": [], "created": "2025-10-23T09:39:17.958Z", "modified": "2025-10-23T09:39:18.049Z"}, {"entity": "publication", "iuid": "0913ce699e3b4b72a5f11076be4ded5b", "links": {"self": {"href": "https://publications.scilifelab.se/publication/0913ce699e3b4b72a5f11076be4ded5b.json"}, "display": {"href": "https://publications.scilifelab.se/publication/0913ce699e3b4b72a5f11076be4ded5b"}}, "title": "Liver Graft Proteomics Reveals Potential Incipient Mechanisms behind Early Renal Dysfunction after Liver Transplantation.", "authors": [{"family": "Nor\u00e9n", "given": "\u00c5sa", "initials": "\u00c5"}, {"family": "Oltean", "given": "Mihai", "initials": "M", "orcid": "0000-0003-3783-5207", "researcher": {"href": "https://publications.scilifelab.se/researcher/60a46e232d2644afbe9a4f3d89316a7a.json"}}, {"family": "Friman", "given": "Styrbj\u00f6rn", "initials": "S"}, {"family": "Molinaro", "given": "Antonio", "initials": "A"}, {"family": "M\u00f6lne", "given": "Johan", "initials": "J"}, {"family": "Sihlbom", "given": "Carina", "initials": "C", "orcid": "0000-0002-1704-2333", "researcher": {"href": "https://publications.scilifelab.se/researcher/4039f4c57baf4052ab7a3f0296d46a7a.json"}}, {"family": "Herlenius", "given": "Gustaf", "initials": "G"}, {"family": "Thorsell", "given": "Annika", "initials": "A"}], "type": "journal article", "published": "2022-10-08", "journal": {"title": "Int J Mol Sci", "issn": "1422-0067", "volume": "23", "issue": "19", "issn-l": null}, "abstract": "Acute kidney injury (AKI) is frequent after liver transplantation (LT) and correlates with later development of chronic kidney disease. Its etiology is multifactorial and combines pre-, intra-, and postoperative factors. Additionally, the liver graft itself seems an important element in the development of AKI, yet the detailed mechanisms remain unclear. We hypothesized that grafts of LT recipients developing significant early AKI may show distinct proteomic alterations, and we set out to identify proteome differences between LT recipients developing moderate or severe AKI (n = 7) and LT recipients without early renal injury (n = 7). Liver biopsies obtained one hour after reperfusion were assessed histologically and using quantitative proteomics. Several cytokines and serum amyloid A2 (SAA2) were analyzed in serum samples obtained preoperatively, 2\u22124 h, and 20\u221224 h after graft reperfusion, respectively. LT induced mild histological alterations without significant differences between groups but uniformly altered liver function tests peaking on postoperative day 1, with a trend towards more severe alterations in patients developing AKI. Global quantitative proteomic analysis revealed 136 proteins differing significantly in their expression levels (p < 0.05, FC 20%): 80 proteins had higher and 56 had lower levels in the AKI group. Most of these proteins were related to immune and inflammatory responses, host defense, and neutrophil degranulation. No differences between the studied pro- and anti-inflammatory cytokines or SAA2 between groups were found at any moment. Our results suggest that grafts of LT patients who develop early AKI reveal a distinct proteome dominated by an early yet prominent activation of the innate immunity. These findings support the hypothesis that AKI after LT may be favored by certain graft characteristics.", "doi": "10.3390/ijms231911929", "pmid": "36233231", "labels": {"Glycoproteomics and MS Proteomics": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC9569532"}, {"db": "pii", "key": "ijms231911929"}], "notes": [], "created": "2023-03-07T14:29:39.657Z", "modified": "2024-01-16T13:46:28.266Z"}]}