{"entity": "researcher", "timestamp": "2026-10-05T21:31:32.205Z", "family": "Savenkov", "given": "Eugene I", "initials": "EI", "orcid": "0000-0002-5802-5089", "affiliations": [], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/5e4c252bb7ec444c935c0aae5387df53.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/5e4c252bb7ec444c935c0aae5387df53"}}, "publications": [{"entity": "publication", "iuid": "eff23dc9016246219df7655c04dbc6c8", "links": {"self": {"href": "https://publications.scilifelab.se/publication/eff23dc9016246219df7655c04dbc6c8.json"}, "display": {"href": "https://publications.scilifelab.se/publication/eff23dc9016246219df7655c04dbc6c8"}}, "title": "Comparative Transcriptome Profiling of Nicotiana benthamiana Plants Infected with Potato Mop-Top Virus and Its Mutant Lacking a Gene for the 8K Protein Underlines the Role of Chloroplasts During Infection.", "authors": [{"family": "Roy", "given": "Shweta", "initials": "S"}, {"family": "Nemes", "given": "Katalin", "initials": "K"}, {"family": "Saripella", "given": "Ganapathi Varma", "initials": "GV"}, {"family": "Vetukuri", "given": "Ramesh Raju", "initials": "RR", "orcid": "0000-0001-7129-5326", "researcher": {"href": "https://publications.scilifelab.se/researcher/cbb5fb93506a4b3a8faacc38397a741c.json"}}, {"family": "Siddique", "given": "Abu Bakar", "initials": "AB"}, {"family": "Savenkov", "given": "Eugene I", "initials": "EI", "orcid": "0000-0002-5802-5089", "researcher": {"href": "https://publications.scilifelab.se/researcher/5e4c252bb7ec444c935c0aae5387df53.json"}}], "type": "journal article", "published": "2026-03-31", "journal": {"title": "Mol. Plant Microbe Interact.", "issn": "0894-0282", "pages": "MPMI10250146R", "issn-l": null}, "abstract": "Potato mop-top virus (PMTV) is a significant pathogen causing potato \"spraing\" disease worldwide. The PMTV 8K protein functions as a weak viral suppressor of RNA silencing (VSR), has viroporin activity, and plays a role in pathogenicity by promoting viral long-distance movement and modulating host responses. Uniquely, PMTV can establish systemic infection in the absence of the 8K protein, though the infection is slightly delayed. To elucidate the molecular mechanisms underlying PMTV-host interactions, we conducted comprehensive RNA-seq analysis comparing wild-type PMTV with a mutant lacking the 8K gene (PMTV-\u03948K) in Nicotiana benthamiana. Our transcriptomic analysis shows that wild-type PMTV and PMTV-\u03948K elicit largely distinct transcriptional responses in the host, with more unique than shared differentially expressed genes. The analysis also revealed extensive reprogramming of metabolic pathways, stress responses, and defense mechanisms. Notably, wild-type PMTV induced more defense-related transcription factors, including 27 WRKY genes compared with 8 in PMTV-\u03948K infections. RNA-silencing pathway genes displayed distinct expression patterns, with AGO2, RDR1, and AGO-MEL1 showing notably enhanced upregulation (up to 9.7-fold) in PMTV-\u03948K infections. Functional analysis identified chloroplast-associated genes GNS2, CHUP1, and KIN5l as host restriction factors. Virus-induced gene silencing experiments confirmed that GNS2 and CHUP1 restrict viral accumulation under both infection scenarios (wild-type PMTV and PMTV-\u03948K), and localization studies revealed that the TGB2 protein and GNS2 colocalize at chloroplast structures. These findings provide insights into PMTV pathogenesis, suggest that 8K is a multifunctional protein operating through diverse mechanisms, and advance understanding of viral suppression strategies. [Formula: see text] Copyright \u00a9 2026 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.", "doi": "10.1094/MPMI-10-25-0146-R", "pmid": "41642887", "labels": {"NGI Uppsala (SNP&SEQ Technology Platform)": "Service", "NGI Short read": "Service", "National Genomics Infrastructure": "Service"}, "xrefs": [], "notes": [], "created": "2026-06-01T11:10:49.177Z", "modified": "2026-06-01T11:10:49.273Z"}, {"entity": "publication", "iuid": "3c2e6b91baaf43ae9efca4b999d27a81", "links": {"self": {"href": "https://publications.scilifelab.se/publication/3c2e6b91baaf43ae9efca4b999d27a81.json"}, "display": {"href": "https://publications.scilifelab.se/publication/3c2e6b91baaf43ae9efca4b999d27a81"}}, "title": "Effect of RNA silencing suppression activity of chrysanthemum virus B p12 protein on small RNA species.", "authors": [{"family": "Vetukuri", "given": "Ramesh R", "initials": "RR"}, {"family": "Kalyandurg", "given": "Pruthvi B", "initials": "PB"}, {"family": "Saripella", "given": "Ganapathi Varma", "initials": "GV"}, {"family": "Sen", "given": "Diya", "initials": "D"}, {"family": "Gil", "given": "Jose Fernando", "initials": "JF"}, {"family": "Lukhovitskaya", "given": "Nina I", "initials": "NI"}, {"family": "Grenville-Briggs", "given": "Laura J", "initials": "LJ"}, {"family": "Savenkov", "given": "Eugene I", "initials": "EI", "orcid": "0000-0002-5802-5089", "researcher": {"href": "https://publications.scilifelab.se/researcher/5e4c252bb7ec444c935c0aae5387df53.json"}}], "type": "journal article", "published": "2020-12-00", "journal": {"title": "Arch Virol", "issn": "1432-8798", "volume": "165", "issue": "12", "pages": "2953-2959", "issn-l": null}, "abstract": "Chrysanthemum virus B encodes a multifunctional p12 protein that acts as a transcriptional activator in the nucleus and as a suppressor of RNA silencing in the cytoplasm. Here, we investigated the impact of p12 on accumulation of major classes of small RNAs (sRNAs). The results show dramatic changes in the sRNA profiles characterised by an overall reduction in sRNA accumulation, changes in the pattern of size distribution of canonical siRNAs and in the ratio between sense and antisense strands, lower abundance of siRNAs with a U residue at the 5'-terminus, and changes in the expression of certain miRNAs, most of which were downregulated.", "doi": "10.1007/s00705-020-04832-y", "pmid": "33040310", "labels": {"NGI Stockholm (Genomics Production)": "Service", "National Genomics Infrastructure": "Service", "NGI Stockholm (Genomics Applications)": "Service"}, "xrefs": [{"db": "pii", "key": "10.1007/s00705-020-04832-y"}, {"db": "pmc", "key": "PMC7588395"}], "notes": [], "created": "2020-12-07T16:32:30.292Z", "modified": "2021-11-10T12:44:41.396Z"}]}