{"entity": "researcher", "timestamp": "2026-07-10T07:56:53.596Z", "family": "Larsson", "given": "Jan", "initials": "J", "orcid": "0000-0003-4373-6790", "affiliations": ["Department of Molecular Biology, Ume\u00e5 University, SE-90187 Ume\u00e5, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/5d6f8e41628d4534879edaf229575dec.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/5d6f8e41628d4534879edaf229575dec"}}, "publications": [{"entity": "publication", "iuid": "56a35d53dd074855a03836ab41cdc335", "links": {"self": {"href": "https://publications.scilifelab.se/publication/56a35d53dd074855a03836ab41cdc335.json"}, "display": {"href": "https://publications.scilifelab.se/publication/56a35d53dd074855a03836ab41cdc335"}}, "title": "Polycomb repression works without Siesta, the Drosophila ortholog of mammalian PCGF3.", "authors": [{"family": "Kahn", "given": "Tatyana G", "initials": "TG", "orcid": "0000-0001-6109-6243", "researcher": {"href": "https://publications.scilifelab.se/researcher/2981d13fc748402cad966852e9d00395.json"}}, {"family": "Garrido", "given": "Andres", "initials": "A", "orcid": "0009-0000-7738-4397", "researcher": {"href": "https://publications.scilifelab.se/researcher/68346f49e1ec4a9f813fc916f7e7fa5d.json"}}, {"family": "Yushkova", "given": "Anastasiya", "initials": "A", "orcid": "0000-0002-9037-6866", "researcher": {"href": "https://publications.scilifelab.se/researcher/93639e0c8c504000a5a2bdf459a149a1.json"}}, {"family": "Kim", "given": "Maria", "initials": "M"}, {"family": "Glotov", "given": "Alexander", "initials": "A"}, {"family": "Sreekumar", "given": "Sweda", "initials": "S"}, {"family": "Larsson", "given": "Jan", "initials": "J", "orcid": "0000-0003-4373-6790", "researcher": {"href": "https://publications.scilifelab.se/researcher/5d6f8e41628d4534879edaf229575dec.json"}}, {"family": "Schwartz", "given": "Yuri B", "initials": "YB", "orcid": "0000-0003-4790-3920", "researcher": {"href": "https://publications.scilifelab.se/researcher/2751a236629d4b8bb9fff42cad6ff614.json"}}], "type": "journal article", "published": "2026-03-06", "journal": {"title": "Sci Adv", "issn": "2375-2548", "volume": "12", "issue": "10", "pages": "eaec0733", "issn-l": "2375-2548"}, "abstract": "Polycomb group proteins mediate epigenetic repression via multisubunit complexes, including canonical Polycomb Repressive Complex 1 (PRC1), which monoubiquitylates histone H2A and binds histone H3 trimethylated at lysine-27 (H3K27me3). The RING1 subunit of PRC1, critical for H2A ubiquitylation, forms other complexes. These variant RING1 complexes also ubiquitylate H2A but cannot bind H3K27me3, and their role in epigenetic repression is debated. Using Drosophila genetics, we found that canonical PRC1 and variant RING1 complexes ubiquitylate H2A at distinct genomic regions. We established that the Drosophila PCGF protein specific for variant RING1 complexes, which we named Siesta, is not required for epigenetic repression of developmental genes but controls larval locomotion independently of H2A ubiquitylation. Leveraging a massively parallel transgenic approach, we demonstrated that H2A ubiquitylation has minimal impact on transcriptional repression. Our findings imply that Siesta-RING1 complexes operate outside the Polycomb regulatory system and that the popular PRC1 classification will benefit from revision.", "doi": "10.1126/sciadv.aec0733", "pmid": "41790891", "labels": {"NGI Uppsala (SNP&SEQ Technology Platform)": "Service", "NGI Short read": "Service", "National Genomics Infrastructure": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC12965321"}], "notes": [], "created": "2026-06-01T11:10:51.338Z", "modified": "2026-06-01T11:10:51.612Z"}, {"entity": "publication", "iuid": "2d9f608223ca4943b796ebbb3a671d0c", "links": {"self": {"href": "https://publications.scilifelab.se/publication/2d9f608223ca4943b796ebbb3a671d0c.json"}, "display": {"href": "https://publications.scilifelab.se/publication/2d9f608223ca4943b796ebbb3a671d0c"}}, "title": "Methylation of lysine 36 on histone H3 is required to control transposon activities in somatic cells.", "authors": [{"family": "Lindehell", "given": "Henrik", "initials": "H", "orcid": "0000-0003-1195-2341", "researcher": {"href": "https://publications.scilifelab.se/researcher/bffc62dbca3145d8849c0dbbb16d44b3.json"}}, {"family": "Schwartz", "given": "Yuri B", "initials": "YB"}, {"family": "Larsson", "given": "Jan", "initials": "J", "orcid": "0000-0003-4373-6790", "researcher": {"href": "https://publications.scilifelab.se/researcher/5d6f8e41628d4534879edaf229575dec.json"}}], "type": "journal article", "published": "2023-08-00", "journal": {"title": "Life Sci. Alliance", "issn": "2575-1077", "volume": "6", "issue": "8", "issn-l": "2575-1077"}, "abstract": "Transposable elements constitute a substantial portion of most eukaryotic genomes and their activity can lead to developmental and neuronal defects. In the germline, transposon activity is antagonized by the PIWI-interacting RNA pathway tasked with repression of transposon transcription and degrading transcripts that have already been produced. However, most of the genes required for transposon control are not expressed outside the germline, prompting the question: what causes deleterious transposons activity in the soma and how is it managed? Here, we show that disruptions of the Histone 3 lysine 36 methylation machinery led to increased transposon transcription in Drosophila melanogaster brains and that there is division of labour for the repression of transposable elements between the different methyltransferases Set2, NSD, and Ash1. Furthermore, we show that disruption of methylation leads to somatic activation of key genes in the PIWI-interacting RNA pathway and the preferential production of RNA from dual-strand piRNA clusters.", "doi": "10.26508/lsa.202201832", "pmid": "37169594", "labels": {"NGI Short read": "Service", "National Genomics Infrastructure": "Service", "NGI Stockholm (Genomics Production)": "Service", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC10176111"}, {"db": "pii", "key": "6/8/e202201832"}], "notes": [], "created": "2023-10-04T12:51:01.650Z", "modified": "2024-01-16T13:48:32.747Z"}, {"entity": "publication", "iuid": "a21aae4fea454a37a843e4ee311e249f", "links": {"self": {"href": "https://publications.scilifelab.se/publication/a21aae4fea454a37a843e4ee311e249f.json"}, "display": {"href": "https://publications.scilifelab.se/publication/a21aae4fea454a37a843e4ee311e249f"}}, "title": "Modulation of RNA stability regulates gene expression in two opposite ways: through buffering of RNA levels upon global perturbations and by supporting adapted differential expression.", "authors": [{"family": "Faucillion", "given": "Marie-Line", "initials": "ML"}, {"family": "Johansson", "given": "Anna-Mia", "initials": "AM"}, {"family": "Larsson", "given": "Jan", "initials": "J", "orcid": "0000-0003-4373-6790", "researcher": {"href": "https://publications.scilifelab.se/researcher/5d6f8e41628d4534879edaf229575dec.json"}}], "type": "journal article", "published": "2022-05-06", "journal": {"title": "Nucleic Acids Res.", "issn": "1362-4962", "issn-l": "0305-1048", "volume": "50", "issue": "8", "pages": "4372-4388"}, "abstract": "The steady state levels of RNAs, often referred to as expression levels, result from a well-balanced combination of RNA transcription and decay. Alterations in RNA levels will therefore result from tight regulation of transcription rates, decay rates or both. Here, we explore the role of RNA stability in achieving balanced gene expression and present genome-wide RNA stabilities in Drosophila melanogaster male and female cells as well as male cells depleted of proteins essential for dosage compensation. We identify two distinct RNA-stability mediated responses involved in regulation of gene expression. The first of these responds to acute and global changes in transcription and thus counteracts potentially harmful gene mis-expression by shifting the RNA stability in the direction opposite to the transcriptional change. The second response enhances inter-individual differential gene expression by adjusting the RNA stability in the same direction as a transcriptional change. Both mechanisms are global, act on housekeeping as well as non-housekeeping genes and were observed in both flies and mammals. Additionally, we show that, in contrast to mammals, modulation of RNA stability does not detectably contribute to dosage compensation of the sex-chromosomes in D. melanogaster.", "doi": "10.1093/nar/gkac208", "pmid": "35390159", "labels": {"National Genomics Infrastructure": "Service", "NGI Stockholm (Genomics Production)": "Service", "NGI Short read": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC9071389"}, {"db": "pii", "key": "6564801"}], "notes": [], "created": "2022-08-19T08:37:16.435Z", "modified": "2023-10-12T11:27:55.185Z"}, {"entity": "publication", "iuid": "8d8a7c48cf414bbd9c480513a1994913", "links": {"self": {"href": "https://publications.scilifelab.se/publication/8d8a7c48cf414bbd9c480513a1994913.json"}, "display": {"href": "https://publications.scilifelab.se/publication/8d8a7c48cf414bbd9c480513a1994913"}}, "title": "The role of H3K36 methylation and associated methyltransferases in chromosome-specific gene regulation.", "authors": [{"family": "Lindehell", "given": "Henrik", "initials": "H", "orcid": "0000-0003-1195-2341", "researcher": {"href": "https://publications.scilifelab.se/researcher/bffc62dbca3145d8849c0dbbb16d44b3.json"}}, {"family": "Glotov", "given": "Alexander", "initials": "A"}, {"family": "Dorafshan", "given": "Eshagh", "initials": "E"}, {"family": "Schwartz", "given": "Yuri B", "initials": "YB", "orcid": "0000-0003-4790-3920", "researcher": {"href": "https://publications.scilifelab.se/researcher/2751a236629d4b8bb9fff42cad6ff614.json"}}, {"family": "Larsson", "given": "Jan", "initials": "J", "orcid": "0000-0003-4373-6790", "researcher": {"href": "https://publications.scilifelab.se/researcher/5d6f8e41628d4534879edaf229575dec.json"}}], "type": "journal article", "published": "2021-10-00", "journal": {"title": "Sci Adv", "issn": "2375-2548", "volume": "7", "issue": "40", "pages": "eabh4390", "issn-l": "2375-2548"}, "abstract": "[Figure: see text].", "doi": "10.1126/sciadv.abh4390", "pmid": "34597135", "labels": {"NGI Stockholm (Genomics Applications)": "Service", "NGI Stockholm (Genomics Production)": "Service", "National Genomics Infrastructure": "Service", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [], "notes": [], "created": "2021-12-06T13:49:37.169Z", "modified": "2024-01-16T13:48:38.326Z"}]}