{"entity": "researcher", "timestamp": "2026-08-11T14:23:14.596Z", "family": "Karampelias", "given": "Christos", "initials": "C", "orcid": "0000-0002-9990-5760", "affiliations": ["Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/509894ee3f0e41eeaf57eb2febc18c70.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/509894ee3f0e41eeaf57eb2febc18c70"}}, "publications": [{"entity": "publication", "iuid": "62243f04b6c04fa1889cea0d14f2fad5", "links": {"self": {"href": "https://publications.scilifelab.se/publication/62243f04b6c04fa1889cea0d14f2fad5.json"}, "display": {"href": "https://publications.scilifelab.se/publication/62243f04b6c04fa1889cea0d14f2fad5"}}, "title": "Examining the liver-pancreas crosstalk reveals a role for the molybdenum cofactor in \u03b2-cell regeneration.", "authors": [{"family": "Karampelias", "given": "Christos", "initials": "C", "orcid": "0000-0002-9990-5760", "researcher": {"href": "https://publications.scilifelab.se/researcher/509894ee3f0e41eeaf57eb2febc18c70.json"}}, {"family": "B\u0103loiu", "given": "Bianca", "initials": "B", "orcid": "0009-0001-8047-3515", "researcher": {"href": "https://publications.scilifelab.se/researcher/9da7b4065fe84b5885d203adc2aaada2.json"}}, {"family": "Rathkolb", "given": "Birgit", "initials": "B"}, {"family": "da Silva-Buttkus", "given": "Patricia", "initials": "P", "orcid": "0000-0003-4705-3399", "researcher": {"href": "https://publications.scilifelab.se/researcher/324af17801cc42e799681595b27c86d5.json"}}, {"family": "Bachar-Wikstr\u00f6m", "given": "Etty", "initials": "E", "orcid": "0000-0002-3283-6721", "researcher": {"href": "https://publications.scilifelab.se/researcher/388692a214e44e9ea7497b397d237bc0.json"}}, {"family": "Marschall", "given": "Susan", "initials": "S"}, {"family": "Fuchs", "given": "Helmut", "initials": "H"}, {"family": "Gailus-Durner", "given": "Valerie", "initials": "V"}, {"family": "Chu", "given": "Lianhe", "initials": "L"}, {"family": "Hrab\u011b de Angelis", "given": "Martin", "initials": "M"}, {"family": "Andersson", "given": "Olov", "initials": "O", "orcid": "0000-0001-6715-781X", "researcher": {"href": "https://publications.scilifelab.se/researcher/f796ff515c95491e9cd47018e02220c0.json"}}], "type": "journal article", "published": "2024-11-00", "journal": {"title": "Life Sci. Alliance", "issn": "2575-1077", "issn-l": "2575-1077", "volume": "7", "issue": "11", "pages": "e202402771"}, "abstract": "Regeneration of insulin-producing \u03b2-cells is an alternative avenue to manage diabetes, and it is crucial to unravel this process in vivo during physiological responses to the lack of \u03b2-cells. Here, we aimed to characterize how hepatocytes can contribute to \u03b2-cell regeneration, either directly or indirectly via secreted proteins or metabolites, in a zebrafish model of \u03b2-cell loss. Using lineage tracing, we show that hepatocytes do not directly convert into \u03b2-cells even under extreme \u03b2-cell ablation conditions. A transcriptomic analysis of isolated hepatocytes after \u03b2-cell ablation displayed altered lipid- and glucose-related processes. Based on the transcriptomics, we performed a genetic screen that uncovers a potential role of the molybdenum cofactor (Moco) biosynthetic pathway in \u03b2-cell regeneration and glucose metabolism in zebrafish. Consistently, molybdenum cofactor synthesis 2 (Mocs2) haploinsufficiency in mice indicated dysregulated glucose metabolism and liver function. Together, our study sheds light on the liver-pancreas crosstalk and suggests that the molybdenum cofactor biosynthesis pathway should be further studied in relation to glucose metabolism and diabetes.", "doi": "10.26508/lsa.202402771", "pmid": "39159974", "labels": {"NGI Short read": null, "NGI Stockholm (Genomics Production)": null, "National Genomics Infrastructure": null, "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC11333758"}, {"db": "pii", "key": "7/11/e202402771"}], "notes": [], "created": "2024-09-30T08:03:57.463Z", "modified": "2025-02-28T14:23:56.417Z"}, {"entity": "publication", "iuid": "145644634f9d45cc8e81be8b9a64ec6c", "links": {"self": {"href": "https://publications.scilifelab.se/publication/145644634f9d45cc8e81be8b9a64ec6c.json"}, "display": {"href": "https://publications.scilifelab.se/publication/145644634f9d45cc8e81be8b9a64ec6c"}}, "title": "Biodistribution of DNA-origami nanostructures in live zebrafish embryos with single-cell resolution", "authors": [{"family": "Kolonelou", "given": "Christina", "initials": "C", "orcid": "0000-0002-5625-5569", "researcher": {"href": "https://publications.scilifelab.se/researcher/10f25477ca214d38a87d4998f6199bc4.json"}}, {"family": "Br\u00e4utigam", "given": "Lars", "initials": "L"}, {"family": "Edwards", "given": "Steven", "initials": "S"}, {"family": "Engstr\u00f6m", "given": "Enya", "initials": "E", "orcid": "0009-0009-6574-0652", "researcher": {"href": "https://publications.scilifelab.se/researcher/d28127bf85bb4581bb77e33e480f61a6.json"}}, {"family": "Dias", "given": "Jos\u00e9 M", "initials": "JM", "orcid": "0000-0002-1402-0323", "researcher": {"href": "https://publications.scilifelab.se/researcher/82499805c5c24c46b8c2ec6427345c89.json"}}, {"family": "Spratt", "given": "Joel", "initials": "J", "orcid": "0000-0002-8720-5011", "researcher": {"href": "https://publications.scilifelab.se/researcher/7614bed2922848859b450473137f9a80.json"}}, {"family": "Karampelias", "given": "Christos", "initials": "C", "orcid": "0000-0002-9990-5760", "researcher": {"href": "https://publications.scilifelab.se/researcher/509894ee3f0e41eeaf57eb2febc18c70.json"}}, {"family": "Wennmalm", "given": "Stefan", "initials": "S", "orcid": "0000-0002-1850-5440", "researcher": {"href": "https://publications.scilifelab.se/researcher/467044d60a724e60b594812747ee2d2a.json"}}, {"family": "Brismar", "given": "Hjalmar", "initials": "H", "orcid": "0000-0003-0578-4003", "researcher": {"href": "https://publications.scilifelab.se/researcher/1ec23336e2ef4e298f340876f1136dce.json"}}, {"family": "Andersson", "given": "Olov", "initials": "O", "orcid": "0000-0001-6715-781X", "researcher": {"href": "https://publications.scilifelab.se/researcher/f796ff515c95491e9cd47018e02220c0.json"}}, {"family": "Teixeira", "given": "Ana I", "initials": "AI", "orcid": "0000-0001-8169-8815", "researcher": {"href": "https://publications.scilifelab.se/researcher/064f9662cf0247ef9737e7dbcfdeb885.json"}}], "type": "posted-content", "published": "2023-12-23", "journal": {"title": "biorxiv", "issn": null, "issn-l": null, "volume": null, "issue": null, "pages": null}, "abstract": null, "doi": "10.1101/2023.12.22.572973", "pmid": null, "labels": {"Integrated Microscopy Technologies Stockholm": "Collaborative"}, "xrefs": [], "notes": [], "created": "2024-01-10T10:12:41.964Z", "modified": "2025-12-18T19:35:36.703Z"}, {"entity": "publication", "iuid": "ad3b96b9f1e04610964e663761b2ac63", "links": {"self": {"href": "https://publications.scilifelab.se/publication/ad3b96b9f1e04610964e663761b2ac63.json"}, "display": {"href": "https://publications.scilifelab.se/publication/ad3b96b9f1e04610964e663761b2ac63"}}, "title": "Reinforcing one-carbon metabolism via folic acid/Folr1 promotes \u03b2-cell differentiation.", "authors": [{"family": "Karampelias", "given": "Christos", "initials": "C", "orcid": "0000-0002-9990-5760", "researcher": {"href": "https://publications.scilifelab.se/researcher/509894ee3f0e41eeaf57eb2febc18c70.json"}}, {"family": "Rezanejad", "given": "Habib", "initials": "H", "orcid": "0000-0003-3067-6602", "researcher": {"href": "https://publications.scilifelab.se/researcher/8759a9d7c6654216b6336f9fb3d0fb92.json"}}, {"family": "Rosko", "given": "Mandy", "initials": "M"}, {"family": "Duan", "given": "Likun", "initials": "L", "orcid": "0000-0003-3293-6682", "researcher": {"href": "https://publications.scilifelab.se/researcher/8ab937d463cc4fc29a5d31eb9e75ac54.json"}}, {"family": "Lu", "given": "Jing", "initials": "J"}, {"family": "Pazzagli", "given": "Laura", "initials": "L", "orcid": "0000-0002-1908-6073", "researcher": {"href": "https://publications.scilifelab.se/researcher/06de06fae02a4de28dbda5456129fc18.json"}}, {"family": "Bertolino", "given": "Philippe", "initials": "P", "orcid": "0000-0001-8064-8269", "researcher": {"href": "https://publications.scilifelab.se/researcher/2fddf1c144e04b80893cdf2c00a7e85b.json"}}, {"family": "Cesta", "given": "Carolyn E", "initials": "CE", "orcid": "0000-0001-5759-9366", "researcher": {"href": "https://publications.scilifelab.se/researcher/804a85a43f0e42e9ba01f7b77a0e02fb.json"}}, {"family": "Liu", "given": "Xiaojing", "initials": "X", "orcid": "0000-0002-4231-6017", "researcher": {"href": "https://publications.scilifelab.se/researcher/550389a9d42447f6a93b0eb67c9ab119.json"}}, {"family": "Korbutt", "given": "Gregory S", "initials": "GS"}, {"family": "Andersson", "given": "Olov", "initials": "O", "orcid": "0000-0001-6715-781X", "researcher": {"href": "https://publications.scilifelab.se/researcher/f796ff515c95491e9cd47018e02220c0.json"}}], "type": "journal article", "published": "2021-06-07", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "12", "issue": "1", "pages": "3362", "issn-l": "2041-1723"}, "abstract": "Diabetes can be caused by an insufficiency in \u03b2-cell mass. Here, we performed a genetic screen in a zebrafish model of \u03b2-cell loss to identify pathways promoting \u03b2-cell regeneration. We found that both folate receptor 1 (folr1) overexpression and treatment with folinic acid, stimulated \u03b2-cell differentiation in zebrafish. Treatment with folinic acid also stimulated \u03b2-cell differentiation in cultures of neonatal pig islets, showing that the effect could be translated to a mammalian system. In both zebrafish and neonatal pig islets, the increased \u03b2-cell differentiation originated from ductal cells. Mechanistically, comparative metabolomic analysis of zebrafish with/without \u03b2-cell ablation and with/without folinic acid treatment indicated \u03b2-cell regeneration could be attributed to changes in the pyrimidine, carnitine, and serine pathways. Overall, our results suggest evolutionarily conserved and previously unknown roles for folic acid and one-carbon metabolism in the generation of \u03b2-cells.", "doi": "10.1038/s41467-021-23673-0", "pmid": "34099692", "labels": {"National Genomics Infrastructure": "Service", "NGI Stockholm (Genomics Production)": "Service", "NGI Stockholm (Genomics Applications)": "Service", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pii", "key": "10.1038/s41467-021-23673-0"}, {"db": "pmc", "key": "PMC8184927"}], "notes": [], "created": "2021-10-01T09:01:03.656Z", "modified": "2024-01-16T13:48:39.535Z"}]}