{"entity": "researcher", "timestamp": "2026-06-15T18:11:52.058Z", "family": "Sjule", "given": "Ellen", "initials": "E", "orcid": "0000-0003-0166-2826", "affiliations": [], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/4e0fd8926ef843a0a8db936df7209107.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/4e0fd8926ef843a0a8db936df7209107"}}, "publications": [{"entity": "publication", "iuid": "9e18ef322a3d4b42a45a7bbe96686990", "links": {"self": {"href": "https://publications.scilifelab.se/publication/9e18ef322a3d4b42a45a7bbe96686990.json"}, "display": {"href": "https://publications.scilifelab.se/publication/9e18ef322a3d4b42a45a7bbe96686990"}}, "title": "Studying macromolecular composition in cell-cell interfaces using 3D membrane reconstitution systems", "authors": [{"family": "Ragaller", "given": "Franziska", "initials": "F", "orcid": "0000-0002-4148-262X", "researcher": {"href": "https://publications.scilifelab.se/researcher/9ead87aca17f476b9febf3f020567c4b.json"}}, {"family": "Schneider", "given": "Amelie Maribel", "initials": "AM"}, {"family": "Sjule", "given": "Ellen", "initials": "E", "orcid": "0000-0003-0166-2826", "researcher": {"href": "https://publications.scilifelab.se/researcher/4e0fd8926ef843a0a8db936df7209107.json"}}, {"family": "Sun", "given": "Renhua", "initials": "R", "orcid": "0000-0002-8203-4946", "researcher": {"href": "https://publications.scilifelab.se/researcher/f639c1a9dca647c0b583ce74ab398f02.json"}}, {"family": "Han", "given": "Xiao", "initials": "X"}, {"family": "Jenkins", "given": "Edward", "initials": "E"}, {"family": "Dustin", "given": "Michael", "initials": "M"}, {"family": "Achour", "given": "Adnane", "initials": "A"}, {"family": "Sezgin", "given": "Erdinc", "initials": "E", "orcid": "0000-0002-4915-388X", "researcher": {"href": "https://publications.scilifelab.se/researcher/34d3b05d68d64f698ff08dc655d2fe26.json"}}], "type": "posted-content", "published": "2025-11-02", "journal": {"title": "biorxiv", "issn": null, "issn-l": null, "volume": null, "issue": null, "pages": null}, "abstract": null, "doi": "10.1101/2025.11.01.686034", "pmid": null, "labels": {"Integrated Microscopy Technologies Stockholm": "Service"}, "xrefs": [], "notes": [], "created": "2025-11-05T10:03:39.995Z", "modified": "2025-12-18T19:00:09.690Z"}, {"entity": "publication", "iuid": "d2c5072fe8644c0b8b5297199b1fd0e5", "links": {"self": {"href": "https://publications.scilifelab.se/publication/d2c5072fe8644c0b8b5297199b1fd0e5.json"}, "display": {"href": "https://publications.scilifelab.se/publication/d2c5072fe8644c0b8b5297199b1fd0e5"}}, "title": "Measuring plasma membrane fluidity using confocal microscopy.", "authors": [{"family": "Carravilla", "given": "Pablo", "initials": "P", "orcid": "0000-0001-6592-7630", "researcher": {"href": "https://publications.scilifelab.se/researcher/b791a8068a724c179b731f0446b1737a.json"}}, {"family": "Andronico", "given": "Luca", "initials": "L"}, {"family": "Schlegel", "given": "Jan", "initials": "J", "orcid": "0000-0003-3159-8079", "researcher": {"href": "https://publications.scilifelab.se/researcher/c94351acc3934c3084f1ed0429a1879a.json"}}, {"family": "Urem", "given": "Yagmur B", "initials": "YB", "orcid": "0009-0008-5053-2191", "researcher": {"href": "https://publications.scilifelab.se/researcher/78c952b0962443199c9be0ce64d48e4e.json"}}, {"family": "Sjule", "given": "Ellen", "initials": "E", "orcid": "0000-0003-0166-2826", "researcher": {"href": "https://publications.scilifelab.se/researcher/4e0fd8926ef843a0a8db936df7209107.json"}}, {"family": "Ragaller", "given": "Franziska", "initials": "F", "orcid": "0000-0002-4148-262X", "researcher": {"href": "https://publications.scilifelab.se/researcher/9ead87aca17f476b9febf3f020567c4b.json"}}, {"family": "Weber", "given": "Florian", "initials": "F", "orcid": "0000-0002-8946-1377", "researcher": {"href": "https://publications.scilifelab.se/researcher/2d4b9aaf4b7041cdbf1d99f3a0d833f2.json"}}, {"family": "Gurdap", "given": "Cenk O", "initials": "CO", "orcid": "0000-0002-5327-2523", "researcher": {"href": "https://publications.scilifelab.se/researcher/9e3e5501e8d84f46884f1001367ab262.json"}}, {"family": "Ascioglu", "given": "Yavuz", "initials": "Y"}, {"family": "Sych", "given": "Taras", "initials": "T", "orcid": "0000-0002-7330-9063", "researcher": {"href": "https://publications.scilifelab.se/researcher/d104a7d8096b4a4ab2a9fe618489fc7e.json"}}, {"family": "Lorent", "given": "Joseph", "initials": "J"}, {"family": "Sezgin", "given": "Erdinc", "initials": "E", "orcid": "0000-0002-4915-388X", "researcher": {"href": "https://publications.scilifelab.se/researcher/34d3b05d68d64f698ff08dc655d2fe26.json"}}], "type": "journal article", "published": "2025-02-19", "journal": {"title": "Nat Protoc", "issn": "1750-2799", "issn-l": null}, "abstract": "Membrane fluidity is a crucial parameter for cellular physiology. Recent evidence suggests that fluidity varies between cell types and states and in diseases. As membrane fluidity has gradually become an important consideration in cell biology and biomedicine, it is essential to have reliable and quantitative ways to measure it in cells. In the past decade, there has been substantial progress both in chemical probes and in imaging tools to make membrane fluidity measurements easier and more reliable. We have recently established a robust pipeline, using confocal imaging and new environment-sensitive probes, that has been successfully used for several studies. Here we present our detailed protocol for membrane fluidity measurement, from labeling to imaging and image analysis. The protocol takes ~4 h and requires basic expertise in cell culture, wet lab and microscopy.", "doi": "10.1038/s41596-024-01122-8", "pmid": "39972239", "labels": {"Integrated Microscopy Technologies Stockholm": "Service"}, "xrefs": [{"db": "pii", "key": "10.1038/s41596-024-01122-8"}], "notes": [], "created": "2025-03-11T12:49:46.696Z", "modified": "2025-03-24T08:20:58.620Z"}, {"entity": "publication", "iuid": "149b1072f1304f8789c9949d7b997492", "links": {"self": {"href": "https://publications.scilifelab.se/publication/149b1072f1304f8789c9949d7b997492.json"}, "display": {"href": "https://publications.scilifelab.se/publication/149b1072f1304f8789c9949d7b997492"}}, "title": "Apoptosis-mediated ADAM10 activation removes a mucin barrier promoting T cell efferocytosis.", "authors": [{"family": "Drexhage", "given": "Linnea Z", "initials": "LZ", "orcid": "0000-0001-7679-4466", "researcher": {"href": "https://publications.scilifelab.se/researcher/0773a617433d46ceb0ea431f60ee59d1.json"}}, {"family": "Zhang", "given": "Shengpan", "initials": "S", "orcid": "0000-0002-1134-7101", "researcher": {"href": "https://publications.scilifelab.se/researcher/dfdfec78321d488eae1c131484d6a1f0.json"}}, {"family": "Dupont", "given": "Maeva", "initials": "M"}, {"family": "Ragaller", "given": "Franziska", "initials": "F", "orcid": "0000-0002-4148-262X", "researcher": {"href": "https://publications.scilifelab.se/researcher/9ead87aca17f476b9febf3f020567c4b.json"}}, {"family": "Sjule", "given": "Ellen", "initials": "E", "orcid": "0000-0003-0166-2826", "researcher": {"href": "https://publications.scilifelab.se/researcher/4e0fd8926ef843a0a8db936df7209107.json"}}, {"family": "Cabezas-Caballero", "given": "Jose", "initials": "J", "orcid": "0000-0001-9871-3428", "researcher": {"href": "https://publications.scilifelab.se/researcher/460c76524cf748efabf39b555b34f9ee.json"}}, {"family": "Deimel", "given": "Lachlan P", "initials": "LP", "orcid": "0000-0003-3803-871X", "researcher": {"href": "https://publications.scilifelab.se/researcher/fc322e4e39da4a769c515a5c77b844cf.json"}}, {"family": "Robertson", "given": "Helen", "initials": "H", "orcid": "0000-0002-9190-8799", "researcher": {"href": "https://publications.scilifelab.se/researcher/e1cbf72e1e184e91917ffe3208e9095c.json"}}, {"family": "Russell", "given": "Rebecca A", "initials": "RA", "orcid": "0000-0002-6517-6483", "researcher": {"href": "https://publications.scilifelab.se/researcher/7ce1e727941a4626823fc4d4db07088c.json"}}, {"family": "Dushek", "given": "Omer", "initials": "O", "orcid": "0000-0001-5847-5226", "researcher": {"href": "https://publications.scilifelab.se/researcher/444272de68ea4e5fb05ca044d92fee24.json"}}, {"family": "Sezgin", "given": "Erdinc", "initials": "E", "orcid": "0000-0002-4915-388X", "researcher": {"href": "https://publications.scilifelab.se/researcher/34d3b05d68d64f698ff08dc655d2fe26.json"}}, {"family": "Karaji", "given": "Niloofar", "initials": "N"}, {"family": "Sattentau", "given": "Quentin J", "initials": "QJ", "orcid": "0000-0001-7170-1937", "researcher": {"href": "https://publications.scilifelab.se/researcher/d78243e447114e179d1aef437cb35717.json"}}], "type": "journal article", "published": "2024-01-15", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "15", "issue": "1", "pages": "541", "issn-l": "2041-1723"}, "abstract": "Efferocytic clearance of apoptotic cells in general, and T cells in particular, is required for tissue and immune homeostasis. Transmembrane mucins are extended glycoproteins highly expressed in the cell glycocalyx that function as a barrier to phagocytosis. Whether and how mucins may be regulated during cell death to facilitate efferocytic corpse clearance is not well understood. Here we show that normal and transformed human T cells express a subset of mucins which are rapidly and selectively removed from the cell surface during apoptosis. This process is mediated by the ADAM10 sheddase, the activity of which is associated with XKR8-catalyzed flipping of phosphatidylserine to the outer leaflet of the plasma membrane. Mucin clearance enhances uptake of apoptotic T cells by macrophages, confirming mucins as an enzymatically-modulatable barrier to efferocytosis. Together these findings demonstrate a glycocalyx regulatory pathway with implications for therapeutic intervention in the clearance of normal and transformed apoptotic T cells.", "doi": "10.1038/s41467-023-44619-8", "pmid": "38225245", "labels": {"Integrated Microscopy Technologies Stockholm": "Service"}, "xrefs": [{"db": "pii", "key": "10.1038/s41467-023-44619-8"}], "notes": [], "created": "2024-01-16T06:21:10.968Z", "modified": "2024-01-16T06:21:11.640Z"}, {"entity": "publication", "iuid": "6f8b796fc932411ca4ac6f38416b8a4e", "links": {"self": {"href": "https://publications.scilifelab.se/publication/6f8b796fc932411ca4ac6f38416b8a4e.json"}, "display": {"href": "https://publications.scilifelab.se/publication/6f8b796fc932411ca4ac6f38416b8a4e"}}, "title": "Quantifying fluorescence lifetime responsiveness of environment sensitive probes for membrane fluidity measurements", "authors": [{"family": "Ragaller", "given": "Franziska", "initials": "F", "orcid": "0000-0002-4148-262X", "researcher": {"href": "https://publications.scilifelab.se/researcher/9ead87aca17f476b9febf3f020567c4b.json"}}, {"family": "Sjule", "given": "Ellen", "initials": "E", "orcid": "0000-0003-0166-2826", "researcher": {"href": "https://publications.scilifelab.se/researcher/4e0fd8926ef843a0a8db936df7209107.json"}}, {"family": "Urem", "given": "Yagmur Balim", "initials": "YB", "orcid": "0009-0008-5053-2191", "researcher": {"href": "https://publications.scilifelab.se/researcher/78c952b0962443199c9be0ce64d48e4e.json"}}, {"family": "Schlegel", "given": "Jan", "initials": "J", "orcid": "0000-0003-3159-8079", "researcher": {"href": "https://publications.scilifelab.se/researcher/c94351acc3934c3084f1ed0429a1879a.json"}}, {"family": "El", "given": "Rojbin", "initials": "R"}, {"family": "Urbancic", "given": "Dunja", "initials": "D"}, {"family": "Urbancic", "given": "Iztok", "initials": "I", "orcid": "0000-0003-3603-6585", "researcher": {"href": "https://publications.scilifelab.se/researcher/426193c07b2b4ab895595d89b8d97a0d.json"}}, {"family": "Blom", "given": "Hans", "initials": "H", "orcid": "0000-0002-5584-9170", "researcher": {"href": "https://publications.scilifelab.se/researcher/3ce356a74dc84e0ea6af85397f11d869.json"}}, {"family": "Sezgin", "given": "Erdinc", "initials": "E", "orcid": "0000-0002-4915-388X", "researcher": {"href": "https://publications.scilifelab.se/researcher/34d3b05d68d64f698ff08dc655d2fe26.json"}}], "type": "posted-content", "published": "2023-10-25", "journal": {"title": "biorxiv", "issn": null, "issn-l": null, "volume": null, "issue": null, "pages": null}, "abstract": null, "doi": "10.1101/2023.10.23.563572", "pmid": null, "labels": {"Integrated Microscopy Technologies Stockholm": "Collaborative"}, "xrefs": [], "notes": [], "created": "2024-01-10T10:29:03.392Z", "modified": "2025-12-18T19:44:49.894Z"}]}