{"entity": "researcher", "timestamp": "2026-08-08T16:49:18.366Z", "family": "Faria", "given": "Rui", "initials": "R", "orcid": "0000-0001-6635-685X", "affiliations": ["InBIO Laborat\u00f3rio Associado, CIBIO, Centro de Investiga\u00e7\u00e3o em Biodiversidade e Recursos Gen\u00e9ticos Universidade do Porto Vair\u00e3o Portugal.", "BIOPOLIS Program in Genomics, Biodiversity and Land Planning, CIBIO Campus de Vair\u00e3o Vair\u00e3o Portugal."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/4dbdd2fff57949148124073f004f74c2.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/4dbdd2fff57949148124073f004f74c2"}}, "publications": [{"entity": "publication", "iuid": "e2ea7c02a3da445f8d79556975eaaf52", "links": {"self": {"href": "https://publications.scilifelab.se/publication/e2ea7c02a3da445f8d79556975eaaf52.json"}, "display": {"href": "https://publications.scilifelab.se/publication/e2ea7c02a3da445f8d79556975eaaf52"}}, "title": "Coupling of twelve putative chromosomal inversions maintains a strong barrier to gene flow between snail ecotypes.", "authors": [{"family": "Le Moan", "given": "Alan", "initials": "A", "orcid": "0000-0002-9124-6844", "researcher": {"href": "https://publications.scilifelab.se/researcher/609a745ce1fb42fea85cc8d55db25acf.json"}}, {"family": "Stankowski", "given": "Sean", "initials": "S", "orcid": "0000-0003-0472-9299", "researcher": {"href": "https://publications.scilifelab.se/researcher/62d9805534164f3ea59cd97a3ad89435.json"}}, {"family": "Rafajlovi\u0107", "given": "Marina", "initials": "M", "orcid": "0000-0003-2177-4622", "researcher": {"href": "https://publications.scilifelab.se/researcher/c19ea9bb89f644e897e85f38a6341684.json"}}, {"family": "Ortega-Martinez", "given": "Olga", "initials": "O", "orcid": "0000-0003-2734-6434", "researcher": {"href": "https://publications.scilifelab.se/researcher/bc84f54ca2cc49bc869cd23adeffc7ac.json"}}, {"family": "Faria", "given": "Rui", "initials": "R", "orcid": "0000-0001-6635-685X", "researcher": {"href": "https://publications.scilifelab.se/researcher/4dbdd2fff57949148124073f004f74c2.json"}}, {"family": "Butlin", "given": "Roger K", "initials": "RK", "orcid": "0000-0003-4736-0954", "researcher": {"href": "https://publications.scilifelab.se/researcher/e510a963ebeb4e8c8af68b10a001a326.json"}}, {"family": "Johannesson", "given": "Kerstin", "initials": "K", "orcid": "0000-0003-0176-7986", "researcher": {"href": "https://publications.scilifelab.se/researcher/a376951d80cd405183f4ff8606df8bbc.json"}}], "type": "journal article", "published": "2024-08-00", "journal": {"title": "Evolution Letters", "issn": "2056-3744", "volume": "8", "issue": "4", "pages": "575-586", "issn-l": "2056-3744"}, "abstract": "Chromosomal rearrangements can lead to the coupling of reproductive barriers, but whether and how they contribute to the completion of speciation remains unclear. Marine snails of the genus Littorina repeatedly form hybrid zones between populations segregating for multiple inversion arrangements, providing opportunities to study their barrier effects. Here, we analyzed 2 adjacent transects across hybrid zones between 2 ecotypes of Littorina fabalis (\"large\" and \"dwarf\") adapted to different wave exposure conditions on a Swedish island. Applying whole-genome sequencing, we found 12 putative inversions on 9 of 17 chromosomes. Nine of the putative inversions reached near differential fixation between the 2 ecotypes, and all were in strong linkage disequilibrium. These inversions cover 20% of the genome and carry 93% of divergent single nucleotide polymorphisms (SNPs). Bimodal hybrid zones in both transects indicated that the 2 ecotypes of Littorina fabalis maintain their genetic and phenotypic integrity following contact. The bimodality reflects the strong coupling between inversion clines and the extension of the barrier effect across the whole genome. Demographic inference suggests that coupling arose during a period of allopatry and has been maintained for > 1,000 generations after secondary contact. Overall, this study shows that the coupling of multiple chromosomal inversions contributes to strong reproductive isolation. Notably, 2 of the putative inversions overlap with inverted genomic regions associated with ecotype differences in a closely related species (Littorina saxatilis), suggesting the same regions, with similar structural variants, repeatedly contribute to ecotype evolution in distinct species.", "doi": "10.1093/evlett/qrae014", "pmid": "39479507", "labels": {"Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC11523631"}, {"db": "pii", "key": "qrae014"}], "notes": [], "created": "2024-11-25T10:23:07.835Z", "modified": "2025-02-28T14:17:31.399Z"}, {"entity": "publication", "iuid": "2ee8b678c1cc4b938cd6947cc5231c97", "links": {"self": {"href": "https://publications.scilifelab.se/publication/2ee8b678c1cc4b938cd6947cc5231c97.json"}, "display": {"href": "https://publications.scilifelab.se/publication/2ee8b678c1cc4b938cd6947cc5231c97"}}, "title": "An allozyme polymorphism is associated with a large chromosomal inversion in the marine snail Littorina fabalis.", "authors": [{"family": "Le Moan", "given": "Alan", "initials": "A", "orcid": "0000-0002-9124-6844", "researcher": {"href": "https://publications.scilifelab.se/researcher/609a745ce1fb42fea85cc8d55db25acf.json"}}, {"family": "Panova", "given": "Marina", "initials": "M", "orcid": "0000-0002-4147-6473", "researcher": {"href": "https://publications.scilifelab.se/researcher/d2398a3ba7fb4cd2b25968f7760b267b.json"}}, {"family": "De Jode", "given": "Aur\u00e9lien", "initials": "A", "orcid": "0000-0003-0428-439X", "researcher": {"href": "https://publications.scilifelab.se/researcher/b1ff392d2a03435795aa8a8a1ee8dd65.json"}}, {"family": "Ortega-Martinez", "given": "Olga", "initials": "O"}, {"family": "Duvetorp", "given": "M\u00e5rten", "initials": "M"}, {"family": "Faria", "given": "Rui", "initials": "R", "orcid": "0000-0001-6635-685X", "researcher": {"href": "https://publications.scilifelab.se/researcher/4dbdd2fff57949148124073f004f74c2.json"}}, {"family": "Butlin", "given": "Roger", "initials": "R", "orcid": "0000-0003-4736-0954", "researcher": {"href": "https://publications.scilifelab.se/researcher/e510a963ebeb4e8c8af68b10a001a326.json"}}, {"family": "Johannesson", "given": "Kerstin", "initials": "K", "orcid": "0000-0003-0176-7986", "researcher": {"href": "https://publications.scilifelab.se/researcher/a376951d80cd405183f4ff8606df8bbc.json"}}], "type": "journal article", "published": "2023-02-00", "journal": {"title": "Evol Appl", "issn": "1752-4571", "volume": "16", "issue": "2", "pages": "279-292", "issn-l": "1752-4571"}, "abstract": "Understanding the genetic targets of natural selection is one of the most challenging goals of population genetics. Some of the earliest candidate genes were identified from associations between allozyme allele frequencies and environmental variation. One such example is the clinal polymorphism in the arginine kinase (Ak) gene in the marine snail Littorina fabalis. While other enzyme loci do not show differences in allozyme frequencies among populations, the Ak alleles are near differential fixation across repeated wave exposure gradients in Europe. Here, we use this case to illustrate how a new sequencing toolbox can be employed to characterize the genomic architecture associated with historical candidate genes. We found that the Ak alleles differ by nine nonsynonymous substitutions, which perfectly explain the different migration patterns of the allozymes during electrophoresis. Moreover, by exploring the genomic context of the Ak gene, we found that the three main Ak alleles are located on different arrangements of a putative chromosomal inversion that reaches near fixation at the opposing ends of two transects covering a wave exposure gradient. This shows Ak is part of a large (3/4 of the chromosome) genomic block of differentiation, in which Ak is unlikely to be the only target of divergent selection. Nevertheless, the nonsynonymous substitutions among Ak alleles and the complete association of one allele with one inversion arrangement suggest that the Ak gene is a strong candidate to contribute to the adaptive significance of the inversion.", "doi": "10.1111/eva.13427", "pmid": "36793696", "labels": {"National Genomics Infrastructure": "Service", "NGI Short read": "Service", "NGI Stockholm (Genomics Production)": "Service", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC9923470"}, {"db": "pii", "key": "EVA13427"}], "notes": [], "created": "2023-10-04T12:04:49.853Z", "modified": "2024-01-16T13:48:34.062Z"}]}