{"entity": "researcher", "timestamp": "2026-08-10T11:34:14.061Z", "family": "Gonz\u00e1lez-L\u00f3pez", "given": "Adri\u00e1n", "initials": "A", "orcid": "0000-0002-4302-6855", "affiliations": ["Department of Cell and Molecular Biology, Uppsala University, BMC, Uppsala, Sweden.", "Uppsala Antibiotic Center, Uppsala University, Uppsala, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/4cdef9bebbf940a5812ce94fa81519e9.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/4cdef9bebbf940a5812ce94fa81519e9"}}, "publications": [{"entity": "publication", "iuid": "d8643fc1bb1a43d3b9fd1f76d1c85fec", "links": {"self": {"href": "https://publications.scilifelab.se/publication/d8643fc1bb1a43d3b9fd1f76d1c85fec.json"}, "display": {"href": "https://publications.scilifelab.se/publication/d8643fc1bb1a43d3b9fd1f76d1c85fec"}}, "title": "Structural mechanism of FusB-mediated rescue from fusidic acid inhibition of protein synthesis.", "authors": [{"family": "Gonz\u00e1lez-L\u00f3pez", "given": "Adri\u00e1n", "initials": "A", "orcid": "0000-0002-4302-6855", "researcher": {"href": "https://publications.scilifelab.se/researcher/4cdef9bebbf940a5812ce94fa81519e9.json"}}, {"family": "Ge", "given": "Xueliang", "initials": "X", "orcid": "0000-0001-7954-3195", "researcher": {"href": "https://publications.scilifelab.se/researcher/5d0da6ede33c4780ba09400c10b80565.json"}}, {"family": "Larsson", "given": "Daniel S D", "initials": "DSD", "orcid": "0000-0001-7683-6419", "researcher": {"href": "https://publications.scilifelab.se/researcher/9e2b9321c1064208b9c0a1b11dde571d.json"}}, {"family": "Sihlbom Wallem", "given": "Carina", "initials": "C"}, {"family": "Sanyal", "given": "Suparna", "initials": "S", "orcid": "0000-0002-7124-792X", "researcher": {"href": "https://publications.scilifelab.se/researcher/c5c5ffabce284f28885f6594da1460d6.json"}}, {"family": "Selmer", "given": "Maria", "initials": "M", "orcid": "0000-0001-9079-2774", "researcher": {"href": "https://publications.scilifelab.se/researcher/860221451df4451b8c035ff4cf25c812.json"}}], "type": "journal article", "published": "2025-04-18", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "16", "issue": "1", "pages": "3693", "issn-l": "2041-1723"}, "abstract": "The antibiotic resistance protein FusB rescues protein synthesis from inhibition by fusidic acid (FA), which locks elongation factor G (EF-G) to the ribosome after GTP hydrolysis. Here, we present time-resolved single-particle cryo-EM structures explaining the mechanism of FusB-mediated rescue. FusB binds to the FA-trapped EF-G on the ribosome, causing large-scale conformational changes of EF-G that break interactions with the ribosome, tRNA, and mRNA. This leads to dissociation of EF-G from the ribosome, followed by FA release. We also observe two independent binding sites of FusB on the classical-state ribosome, overlapping with the binding site of EF-G to each of the ribosomal subunits, yet not inhibiting tRNA delivery. The affinity of FusB to the ribosome and the concentration of FusB in S. aureus during FusB-mediated resistance support that direct binding of FusB to ribosomes could occur in the cell. Our results reveal an intricate resistance mechanism involving specific interactions of FusB with both EF-G and the ribosome, and a non-canonical release pathway of EF-G.", "doi": "10.1038/s41467-025-58902-3", "pmid": "40251147", "labels": {"Cryo-EM": "Service", "Glycoproteomics and MS Proteomics": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC12008383"}, {"db": "pii", "key": "10.1038/s41467-025-58902-3"}], "notes": [], "created": "2025-04-24T09:33:19.909Z", "modified": "2025-10-29T14:59:52.946Z"}, {"entity": "publication", "iuid": "23e2b6a4fd7641269a2145e9df329eec", "links": {"self": {"href": "https://publications.scilifelab.se/publication/23e2b6a4fd7641269a2145e9df329eec.json"}, "display": {"href": "https://publications.scilifelab.se/publication/23e2b6a4fd7641269a2145e9df329eec"}}, "title": "Structures of theStaphylococcus aureusribosome inhibited by fusidic acid and fusidic acid cyclopentane", "authors": [{"family": "Gonz\u00e1lez-L\u00f3pez", "given": "Adri\u00e1n", "initials": "A", "orcid": "0000-0002-4302-6855", "researcher": {"href": "https://publications.scilifelab.se/researcher/4cdef9bebbf940a5812ce94fa81519e9.json"}}, {"family": "Larsson", "given": "Daniel S D", "initials": "DSD", "orcid": "0000-0001-7683-6419", "researcher": {"href": "https://publications.scilifelab.se/researcher/9e2b9321c1064208b9c0a1b11dde571d.json"}}, {"family": "Koripella", "given": "Ravi Kiran", "initials": "RK"}, {"family": "Cain", "given": "Brett N", "initials": "BN"}, {"family": "Chavez", "given": "Martin Garcia", "initials": "MG"}, {"family": "Hergenrother", "given": "Paul J", "initials": "PJ"}, {"family": "Sanyal", "given": "Suparna", "initials": "S", "orcid": "0000-0002-7124-792X", "researcher": {"href": "https://publications.scilifelab.se/researcher/c5c5ffabce284f28885f6594da1460d6.json"}}, {"family": "Selmer", "given": "Maria", "initials": "M", "orcid": "0000-0001-9079-2774", "researcher": {"href": "https://publications.scilifelab.se/researcher/860221451df4451b8c035ff4cf25c812.json"}}], "type": "posted-content", "published": "2023-11-20", "journal": {"title": "biorxiv", "issn": null, "issn-l": null, "volume": null, "issue": null, "pages": null}, "abstract": null, "doi": "10.1101/2023.11.17.567578", "pmid": null, "labels": {"Cryo-EM": "Service"}, "xrefs": [], "notes": [], "created": "2023-11-27T10:38:38.547Z", "modified": "2025-12-18T19:41:02.048Z"}]}