{"entity": "researcher", "timestamp": "2026-06-08T05:45:08.090Z", "family": "Huoman", "given": "Johanna", "initials": "J", "orcid": "0000-0003-2509-2418", "affiliations": [], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/471444d05e834a9b86a5a6d187da2c04.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/471444d05e834a9b86a5a6d187da2c04"}}, "publications": [{"entity": "publication", "iuid": "beb46404be78423ebb108a8aed3fceee", "links": {"self": {"href": "https://publications.scilifelab.se/publication/beb46404be78423ebb108a8aed3fceee.json"}, "display": {"href": "https://publications.scilifelab.se/publication/beb46404be78423ebb108a8aed3fceee"}}, "title": "Combined prenatal Lactobacillus reuteri and \u03c9-3 supplementation synergistically modulates DNA methylation in neonatal T helper cells.", "authors": [{"family": "Huoman", "given": "Johanna", "initials": "J", "orcid": "0000-0003-2509-2418", "researcher": {"href": "https://publications.scilifelab.se/researcher/471444d05e834a9b86a5a6d187da2c04.json"}}, {"family": "Mart\u00ednez-Enguita", "given": "David", "initials": "D"}, {"family": "Olsson", "given": "Elin", "initials": "E"}, {"family": "Ernerudh", "given": "Jan", "initials": "J"}, {"family": "Nilsson", "given": "Lennart", "initials": "L"}, {"family": "Duch\u00e9n", "given": "Karel", "initials": "K"}, {"family": "Gustafsson", "given": "Mika", "initials": "M"}, {"family": "Jenmalm", "given": "Maria C", "initials": "MC"}], "type": "journal article", "published": "2021-06-30", "journal": {"title": "Clin Epigenetics", "issn": "1868-7083", "issn-l": "1868-7075", "volume": "13", "issue": "1", "pages": "135"}, "abstract": "Environmental exposures may alter DNA methylation patterns of T helper cells. As T helper cells are instrumental for allergy development, changes in methylation patterns may constitute a mechanism of action for allergy preventive interventions. While epigenetic effects of separate perinatal probiotic or \u03c9-3 fatty acid supplementation have been studied previously, the combined treatment has not been assessed. We aimed to investigate epigenome-wide DNA methylation patterns from a sub-group of children in an on-going randomised double-blind placebo-controlled allergy prevention trial using pre- and postnatal combined Lactobacillus reuteri and \u03c9-3 fatty acid treatment. To this end, > 866000 CpG sites (MethylationEPIC 850K array) in cord blood CD4+ T cells were examined in samples from all four study arms (double-treatment: n = 18, single treatments: probiotics n = 16, \u03c9-3 n = 15, and double placebo: n = 14). Statistical and bioinformatic analyses identified treatment-associated differentially methylated CpGs and genes, which were used to identify putatively treatment-induced network modules. Pathway analyses inferred biological relevance, and comparisons were made to an independent allergy data set.\r\n\r\nComparing the active treatments to the double placebo group, most differentially methylated CpGs and genes were hypermethylated, possibly suggesting induction of transcriptional inhibition. The double-treated group showed the largest number of differentially methylated CpGs, of which many were unique, suggesting synergy between interventions. Clusters within the double-treated network module consisted of immune-related pathways, including T cell receptor signalling, and antigen processing and presentation, with similar pathways revealed for the single-treatment modules. CpGs derived from differential methylation and network module analyses were enriched in an independent allergy data set, particularly in the double-treatment group, proposing treatment-induced DNA methylation changes as relevant for allergy development.\r\n\r\nPrenatal L. reuteri and/or \u03c9-3 fatty acid treatment results in hypermethylation and affects immune- and allergy-related pathways in neonatal T helper cells, with potentially synergistic effects between the interventions and relevance for allergic disease. Further studies need to address these findings on a transcriptional level, and whether the results associate to allergy development in the children. Understanding the role of DNA methylation in regulating effects of perinatal probiotic and \u03c9-3 interventions may provide essential knowledge in the development of efficacious allergy preventive strategies. Trial registration ClinicalTrials.gov, ClinicalTrials.gov-ID: NCT01542970. Registered 27th of February 2012-Retrospectively registered, https://clinicaltrials.gov/ct2/show/NCT01542970 .", "doi": "10.1186/s13148-021-01115-4", "pmid": "34193262", "labels": {"NGI Uppsala (SNP&SEQ Technology Platform)": "Service", "National Genomics Infrastructure": "Service"}, "xrefs": [{"db": "pii", "key": "10.1186/s13148-021-01115-4"}, {"db": "pmc", "key": "PMC8247185"}, {"db": "ClinicalTrials.gov", "key": "NCT01542970"}], "notes": [], "created": "2021-08-19T13:41:37.013Z", "modified": "2021-12-07T13:21:25.193Z"}, {"entity": "publication", "iuid": "f005a36d6a6944bc939d90904088154d", "links": {"self": {"href": "https://publications.scilifelab.se/publication/f005a36d6a6944bc939d90904088154d.json"}, "display": {"href": "https://publications.scilifelab.se/publication/f005a36d6a6944bc939d90904088154d"}}, "title": "Pre- and postnatal Lactobacillus reuteri treatment alters DNA methylation of infant T helper cells.", "authors": [{"family": "Forsberg", "given": "Anna", "initials": "A"}, {"family": "Huoman", "given": "Johanna", "initials": "J", "orcid": "0000-0003-2509-2418", "researcher": {"href": "https://publications.scilifelab.se/researcher/471444d05e834a9b86a5a6d187da2c04.json"}}, {"family": "S\u00f6derholm", "given": "Simon", "initials": "S", "orcid": "0000-0001-5350-7102", "researcher": {"href": "https://publications.scilifelab.se/researcher/75f4744864904171b6a708e7a8d2b471.json"}}, {"family": "Bhai Mehta", "given": "Ratnesh", "initials": "R"}, {"family": "Nilsson", "given": "Lennart", "initials": "L", "orcid": "0000-0002-5680-6367", "researcher": {"href": "https://publications.scilifelab.se/researcher/19f74feeb54c4d588b879ea9e8843662.json"}}, {"family": "Abrahamsson", "given": "Thomas R", "initials": "TR"}, {"family": "Ernerudh", "given": "Jan", "initials": "J"}, {"family": "Gustafsson", "given": "Mika", "initials": "M"}, {"family": "Jenmalm", "given": "Maria C", "initials": "MC", "orcid": "0000-0002-2117-5366", "researcher": {"href": "https://publications.scilifelab.se/researcher/bf1f485192744e0c95ccecdb5471b577.json"}}], "type": "journal article", "published": "2020-07-00", "journal": {"title": "Pediatr Allergy Immunol", "issn": "1399-3038", "volume": "31", "issue": "5", "pages": "544-553", "issn-l": "0905-6157"}, "abstract": "Perinatal childhood exposures, including probiotic supplementation, may affect epigenetic modifications and impact on immune maturation and allergy development. The aim of this study was to assess the effects of pre- and postnatal Lactobacillus reuteri supplementation on DNA methylation in relation to immune maturation and allergy development.\n\nDNA methylation patterns were investigated for allergy-related T helper subsets using a locus-specific method and at a genome-wide scale using the Illumina 450K array. From a randomised, double-blind, placebo-controlled allergy prevention trial with pre- and postnatal probiotic supplementation, CD4+ T helper cells were obtained at birth (from cord blood), and 12 and 24 months of age (total (placebo/probiotics); locus-specific method: CB = 32 (17/15), 12 months = 24 (9/15), 24 months = 35 (15/20); Illumina: CB = 19 (10/9), 12 months = 10 (6/4), 24 months = 19(11/8)).\n\nComparing probiotics to placebo, the greatest genome-wide differential DNA methylation was observed at birth, where the majority of sites were hypomethylated, indicating transcriptional accessibility in the probiotic group. Bioinformatic analyses, including network analyses, revealed a module containing 91 genes, enriched for immune-related pathways such as chemotaxis, PI3K-Akt, MAPK and TGF-\u03b2 signalling. A majority of the module genes were associated with atopic manifestations (OR = 1.43, P = 2.4 \u00d7 10-6 ), and a classifier built on this model could predict allergy development (AUC = 0.78, P = 3.0 \u00d7 10e-3 ). Pathways such as IFN-\u03b3 signalling and T-cell activation were more hypermethylated at birth compared with later in life in both intervention groups over time, in line with DNA methylation patterns in the IFNG locus obtained by the locus-specific methodology.\n\nMaternal L. reuteri supplementation during pregnancy alters DNA methylation patterns in CD4+ T cells towards enhanced immune activation at birth, which may affect immune maturation and allergy development.", "doi": "10.1111/pai.13240", "pmid": "32150651", "labels": {"National Genomics Infrastructure": "Service", "NGI Uppsala (SNP&SEQ Technology Platform)": "Service", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [], "notes": [], "created": "2020-03-12T15:01:36.562Z", "modified": "2024-01-16T13:48:42.269Z"}]}