{"entity": "researcher", "timestamp": "2026-08-07T18:35:33.889Z", "family": "Anastasina", "given": "Maria", "initials": "M", "orcid": "0000-0002-4555-6446", "affiliations": ["Faculty of Biological and Environmental Sciences, Molecular and Integrative Bioscience Research Programme, University of Helsinki, 00014 Helsinki, Finland.", "Helsinki Institute of Life Sciences-Institute of Biotechnology, University of Helsinki, 00014 Helsinki, Finland."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/461d4e00b7e44cd682c5ae75825cc557.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/461d4e00b7e44cd682c5ae75825cc557"}}, "publications": [{"entity": "publication", "iuid": "fff9cfaa1b8e46869b41fe1709016ab4", "links": {"self": {"href": "https://publications.scilifelab.se/publication/fff9cfaa1b8e46869b41fe1709016ab4.json"}, "display": {"href": "https://publications.scilifelab.se/publication/fff9cfaa1b8e46869b41fe1709016ab4"}}, "title": "The structure of immature tick-borne encephalitis virus", "authors": [{"family": "Anastasina", "given": "Maria", "initials": "M", "orcid": "0000-0002-4555-6446", "researcher": {"href": "https://publications.scilifelab.se/researcher/461d4e00b7e44cd682c5ae75825cc557.json"}}, {"family": "F\u00fczik", "given": "Tibor", "initials": "T", "orcid": "0000-0002-1190-0210", "researcher": {"href": "https://publications.scilifelab.se/researcher/512bd48ba67341db8f1c43fb680b3b93.json"}}, {"family": "Domanska", "given": "Au\u0161ra", "initials": "A", "orcid": "0000-0002-3772-6003", "researcher": {"href": "https://publications.scilifelab.se/researcher/6b6fec96ed5c4cd88389e71a4fa2412b.json"}}, {"family": "Pulkkinen", "given": "Lauri IA", "initials": "LI", "orcid": "0000-0002-3919-2498", "researcher": {"href": "https://publications.scilifelab.se/researcher/dc4ee78f5bb24fe894f45f721f076a02.json"}}, {"family": "\u0160merdov\u00e1", "given": "Lenka", "initials": "L", "orcid": "0000-0001-8043-0921", "researcher": {"href": "https://publications.scilifelab.se/researcher/d621dd2f1f0242a697cf626506dcb1bb.json"}}, {"family": "Formanov\u00e1", "given": "Petra Pokorn\u00e1", "initials": "PP"}, {"family": "Strakov\u00e1", "given": "Petra", "initials": "P", "orcid": "0000-0002-3130-933X", "researcher": {"href": "https://publications.scilifelab.se/researcher/f303cbf5abdb480ea42f05bbdf3381b3.json"}}, {"family": "Nov\u00e1\u010dek", "given": "Ji\u0159\u00ed", "initials": "J", "orcid": "0000-0003-4013-3898", "researcher": {"href": "https://publications.scilifelab.se/researcher/ea192c0c03b24e46b13c795faac4e60d.json"}}, {"family": "R\u016f\u017eek", "given": "Daniel", "initials": "D", "orcid": "0000-0003-4655-2380", "researcher": {"href": "https://publications.scilifelab.se/researcher/2c83b18f883942c9aeedbf34aa13e7af.json"}}, {"family": "Plevka", "given": "Pavel", "initials": "P", "orcid": "0000-0003-4215-3315", "researcher": {"href": "https://publications.scilifelab.se/researcher/1aaf981bd3c94c0c949d07a3ef7c24b7.json"}}, {"family": "Butcher", "given": "Sarah J", "initials": "SJ", "orcid": "0000-0001-7060-5871", "researcher": {"href": "https://publications.scilifelab.se/researcher/f7076e70172b45d7ab9de4ab86775ae9.json"}}], "type": "posted-content", "published": "2023-08-04", "journal": {"title": "biorxiv", "issn": null, "issn-l": null, "volume": null, "issue": null, "pages": null}, "abstract": null, "doi": "10.1101/2023.08.04.551633", "pmid": null, "labels": {"Cryo-EM": "Service"}, "xrefs": [], "notes": [], "created": "2023-11-26T14:09:21.657Z", "modified": "2025-12-18T19:48:13.455Z"}, {"entity": "publication", "iuid": "33a19acdc3f6449db2df3f83d1a30b3d", "links": {"self": {"href": "https://publications.scilifelab.se/publication/33a19acdc3f6449db2df3f83d1a30b3d.json"}, "display": {"href": "https://publications.scilifelab.se/publication/33a19acdc3f6449db2df3f83d1a30b3d"}}, "title": "Molecular Organisation of Tick-Borne Encephalitis Virus.", "authors": [{"family": "Pulkkinen", "given": "Lauri I A", "initials": "LIA", "orcid": "0000-0002-3919-2498", "researcher": {"href": "https://publications.scilifelab.se/researcher/dc4ee78f5bb24fe894f45f721f076a02.json"}}, {"family": "Barrass", "given": "Sarah V", "initials": "SV", "orcid": "0000-0001-7319-6268", "researcher": {"href": "https://publications.scilifelab.se/researcher/9acb6c8e408e4ff4a01e0af56a6cb5e3.json"}}, {"family": "Domanska", "given": "Au\u0161ra", "initials": "A", "orcid": "0000-0002-3772-6003", "researcher": {"href": "https://publications.scilifelab.se/researcher/6b6fec96ed5c4cd88389e71a4fa2412b.json"}}, {"family": "\u00d6verby", "given": "Anna K", "initials": "AK", "orcid": "0000-0001-6553-0940", "researcher": {"href": "https://publications.scilifelab.se/researcher/506b0e2b2d884f868df73c7663b9ffb7.json"}}, {"family": "Anastasina", "given": "Maria", "initials": "M", "orcid": "0000-0002-4555-6446", "researcher": {"href": "https://publications.scilifelab.se/researcher/461d4e00b7e44cd682c5ae75825cc557.json"}}, {"family": "Butcher", "given": "Sarah J", "initials": "SJ", "orcid": "0000-0001-7060-5871", "researcher": {"href": "https://publications.scilifelab.se/researcher/f7076e70172b45d7ab9de4ab86775ae9.json"}}], "type": "journal article", "published": "2022-04-11", "journal": {"title": "Viruses", "issn": "1999-4915", "volume": "14", "issue": "4", "issn-l": "1999-4915"}, "abstract": "Tick-borne encephalitis virus (TBEV) is a pathogenic, enveloped, positive-stranded RNA virus in the family Flaviviridae. Structural studies of flavivirus virions have primarily focused on mosquito-borne species, with only one cryo-electron microscopy (cryo-EM) structure of a tick-borne species published. Here, we present a 3.3 \u00c5 cryo-EM structure of the TBEV virion of the Kuutsalo-14 isolate, confirming the overall organisation of the virus. We observe conformational switching of the peripheral and transmembrane helices of M protein, which can explain the quasi-equivalent packing of the viral proteins and highlights their importance in stabilising membrane protein arrangement in the virion. The residues responsible for M protein interactions are highly conserved in TBEV but not in the structurally studied Hypr strain, nor in mosquito-borne flaviviruses. These interactions may compensate for the lower number of hydrogen bonds between E proteins in TBEV compared to the mosquito-borne flaviviruses. The structure reveals two lipids bound in the E protein which are important for virus assembly. The lipid pockets are comparable to those recently described in mosquito-borne Zika, Spondweni, Dengue, and Usutu viruses. Our results thus advance the understanding of tick-borne flavivirus architecture and virion-stabilising interactions.", "doi": "10.3390/v14040792", "pmid": "35458522", "labels": {"Cryo-EM": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC9027435"}, {"db": "pii", "key": "v14040792"}], "notes": [], "created": "2023-11-26T14:08:02.582Z", "modified": "2023-11-26T14:08:02.704Z"}]}