{"entity": "researcher", "timestamp": "2026-07-22T17:07:23.731Z", "family": "Stepanauskaite", "given": "Lina", "initials": "L", "orcid": "0000-0003-4173-6009", "affiliations": ["Science for Life Laboratory, Department of Protein Science, KTH Royal Institute of Technology, 17121, Solna, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/429f33fed9a44ff4b70ff88d5af917d9.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/429f33fed9a44ff4b70ff88d5af917d9"}}, "publications": [{"entity": "publication", "iuid": "92139d604e30428c85b0f16ddccb99c8", "links": {"self": {"href": "https://publications.scilifelab.se/publication/92139d604e30428c85b0f16ddccb99c8.json"}, "display": {"href": "https://publications.scilifelab.se/publication/92139d604e30428c85b0f16ddccb99c8"}}, "title": "Spatial profiling of the mouse colonic immune landscape associated with colitis and sex.", "authors": [{"family": "Holm", "given": "Matilda", "initials": "M"}, {"family": "Stepanauskait\u0117", "given": "Lina", "initials": "L", "orcid": "0000-0003-4173-6009", "researcher": {"href": "https://publications.scilifelab.se/researcher/429f33fed9a44ff4b70ff88d5af917d9.json"}}, {"family": "B\u00e4ckstr\u00f6m", "given": "Anna", "initials": "A"}, {"family": "Birgersson", "given": "Madeleine", "initials": "M", "orcid": "0000-0002-5876-0710", "researcher": {"href": "https://publications.scilifelab.se/researcher/68ea3a27e23a4f978e9c4e74ebdfbf11.json"}}, {"family": "Socciarelli", "given": "Fabio", "initials": "F"}, {"family": "Archer", "given": "Amena", "initials": "A", "orcid": "0000-0002-0400-4151", "researcher": {"href": "https://publications.scilifelab.se/researcher/4502538fe3e84cb6a6618c972fa10b08.json"}}, {"family": "Stadler", "given": "Charlotte", "initials": "C", "orcid": "0000-0002-6781-1938", "researcher": {"href": "https://publications.scilifelab.se/researcher/2db3b27c7d7143cbacc8c1dd8ac90a31.json"}}, {"family": "Williams", "given": "Cecilia", "initials": "C", "orcid": "0000-0002-0602-2062", "researcher": {"href": "https://publications.scilifelab.se/researcher/89989da8d4e64dd3a30b87fc62ceefae.json"}}], "type": "journal article", "published": "2024-11-29", "journal": {"title": "Commun Biol", "issn": "2399-3642", "issn-l": "2399-3642", "volume": "7", "issue": "1", "pages": "1595"}, "abstract": "Inflammatory intestinal conditions are a major disease burden. Numerous factors shape the distribution of immune cells in the colon, but a spatial characterization of the homeostatic and inflamed colonic immune microenvironment is lacking. Here, we use the COMET platform for multiplex immunofluorescence to profile the infiltration of nine immune cell populations in mice of both sexes (N = 16) with full spatial context, including in regions of squamous metaplasia. Unsupervised clustering, neighborhood analysis, and manual quantification along the proximal-distal axis characterized the colonic immune landscape, quantified cell-cell interactions, and revealed sex differences. The distal colon was the most affected region during colitis, which was pronounced in males, who exhibited a sex-dependent increase of B cells and reduction of M2-like macrophages. Regions of squamous metaplasia exhibited strong infiltration of numerous immune cell populations, especially in males. Females exhibited more helper T cells and neutrophils at homeostasis and increased M2-like macrophage infiltration in the mid-colon upon colitis. Sex differences were corroborated by plasma cytokine profiles. Our results provide a foundation for future studies of inflammatory intestinal conditions.", "doi": "10.1038/s42003-024-07276-1", "pmid": "39613949", "labels": {"Affinity Proteomics Stockholm": "Service", "Spatial Proteomics": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC11606951"}, {"db": "pii", "key": "10.1038/s42003-024-07276-1"}], "notes": [], "created": "2024-12-04T20:14:13.972Z", "modified": "2025-02-17T09:58:33.843Z"}, {"entity": "publication", "iuid": "923e09636e01418bba0d80b842c05813", "links": {"self": {"href": "https://publications.scilifelab.se/publication/923e09636e01418bba0d80b842c05813.json"}, "display": {"href": "https://publications.scilifelab.se/publication/923e09636e01418bba0d80b842c05813"}}, "title": "High-fat diet and estrogen modulate the gut microbiota in a sex-dependent manner in mice.", "authors": [{"family": "Hases", "given": "Linnea", "initials": "L", "orcid": "0000-0001-6741-7204", "researcher": {"href": "https://publications.scilifelab.se/researcher/eeb5e09b88e94ab59a6ca9f8c457c5f4.json"}}, {"family": "Stepanauskaite", "given": "Lina", "initials": "L", "orcid": "0000-0003-4173-6009", "researcher": {"href": "https://publications.scilifelab.se/researcher/429f33fed9a44ff4b70ff88d5af917d9.json"}}, {"family": "Birgersson", "given": "Madeleine", "initials": "M", "orcid": "0000-0002-5876-0710", "researcher": {"href": "https://publications.scilifelab.se/researcher/68ea3a27e23a4f978e9c4e74ebdfbf11.json"}}, {"family": "Brusselaers", "given": "Nele", "initials": "N"}, {"family": "Schuppe-Koistinen", "given": "Ina", "initials": "I"}, {"family": "Archer", "given": "Amena", "initials": "A", "orcid": "0000-0002-0400-4151", "researcher": {"href": "https://publications.scilifelab.se/researcher/4502538fe3e84cb6a6618c972fa10b08.json"}}, {"family": "Engstrand", "given": "Lars", "initials": "L"}, {"family": "Williams", "given": "Cecilia", "initials": "C", "orcid": "0000-0002-0602-2062", "researcher": {"href": "https://publications.scilifelab.se/researcher/89989da8d4e64dd3a30b87fc62ceefae.json"}}], "type": "journal article", "published": "2023-01-09", "journal": {"title": "Commun Biol", "issn": "2399-3642", "volume": "6", "issue": "1", "pages": "20", "issn-l": "2399-3642"}, "abstract": "A high-fat diet can lead to gut microbiota dysbiosis, chronic intestinal inflammation, and metabolic syndrome. Notably, resulting phenotypes, such as glucose and insulin levels, colonic crypt cell proliferation, and macrophage infiltration, exhibit sex differences, and females are less affected. This is, in part, attributed to sex hormones. To investigate if there are sex differences in the microbiota and if estrogenic ligands can attenuate high-fat diet-induced dysbiosis, we used whole-genome shotgun sequencing to characterize the impact of diet, sex, and estrogenic ligands on the microbial composition of the cecal content of mice. We here report clear host sex differences along with remarkably sex-dependent responses to high-fat diet. Females, specifically, exhibited increased abundance of Blautia hansenii, and its levels correlated negatively with insulin levels in both sexes. Estrogen treatment had a modest impact on the microbiota diversity but altered a few important species in males. This included Collinsella aerofaciens F, which we show correlated with colonic macrophage infiltration. In conclusion, male and female mice exhibit clear differences in their cecal microbial composition and in how diet and estrogens impact the composition. Further, specific microbial strains are significantly correlated with metabolic parameters.", "doi": "10.1038/s42003-022-04406-5", "pmid": "36624306", "labels": {"Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC9829864"}, {"db": "pii", "key": "10.1038/s42003-022-04406-5"}], "notes": [], "created": "2023-11-27T21:49:55.417Z", "modified": "2024-01-16T13:48:34.180Z"}]}