{"entity": "researcher", "timestamp": "2026-08-15T06:51:03.268Z", "family": "Trompet", "given": "Dana", "initials": "D", "orcid": "0000-0001-9472-6184", "affiliations": ["Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, Sahlgrenska Osteoporosis Centre, Centre for Bone and Arthritis Research at the Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.", "Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/3ef4d02ec2e1427088522df2a759bf3a.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/3ef4d02ec2e1427088522df2a759bf3a"}}, "publications": [{"entity": "publication", "iuid": "0e45658433fb4fd68d5f344433957d24", "links": {"self": {"href": "https://publications.scilifelab.se/publication/0e45658433fb4fd68d5f344433957d24.json"}, "display": {"href": "https://publications.scilifelab.se/publication/0e45658433fb4fd68d5f344433957d24"}}, "title": "A transcriptional atlas of the pubertal human growth plate reveals two populations of stem cells and direct effect of growth hormone.", "authors": [{"family": "Chu", "given": "Nelson Tsz Long", "initials": "NTL", "orcid": "0000-0001-8553-6880", "researcher": {"href": "https://publications.scilifelab.se/researcher/771e0a6c700f4b4f9765f9a2811608eb.json"}}, {"family": "Dregval", "given": "Ostap", "initials": "O", "orcid": "0000-0002-9939-6492", "researcher": {"href": "https://publications.scilifelab.se/researcher/2a3d8ecff20444e9bf82f702e21e736c.json"}}, {"family": "Zaman", "given": "Farasat", "initials": "F", "orcid": "0000-0002-1832-1051", "researcher": {"href": "https://publications.scilifelab.se/researcher/9c082df96e5c43afbca28c5c6f8e7810.json"}}, {"family": "Li", "given": "Lei", "initials": "L", "orcid": "0000-0002-8026-3665", "researcher": {"href": "https://publications.scilifelab.se/researcher/31e881d87f094a92bc6b34f734ffe12f.json"}}, {"family": "Tian", "given": "Xin", "initials": "X"}, {"family": "Liu", "given": "Xin", "initials": "X"}, {"family": "Trompet", "given": "Dana", "initials": "D", "orcid": "0000-0001-9472-6184", "researcher": {"href": "https://publications.scilifelab.se/researcher/3ef4d02ec2e1427088522df2a759bf3a.json"}}, {"family": "Zhou", "given": "Baoyi", "initials": "B"}, {"family": "Heinonen", "given": "Jussi O", "initials": "JO"}, {"family": "Ohlsson", "given": "Claes", "initials": "C", "orcid": "0000-0002-9633-2805", "researcher": {"href": "https://publications.scilifelab.se/researcher/995dac358caa4a169fc889b7a3eef44a.json"}}, {"family": "S\u00e4vendahl", "given": "Lars", "initials": "L", "orcid": "0000-0003-1067-4976", "researcher": {"href": "https://publications.scilifelab.se/researcher/6da067b5b4a34625806e9335fa921f09.json"}}, {"family": "Adameyko", "given": "Igor", "initials": "I", "orcid": "0000-0001-5471-0356", "researcher": {"href": "https://publications.scilifelab.se/researcher/346f484a56cb4ad5b866b194ccd44e4f.json"}}, {"family": "Chagin", "given": "Andrei S", "initials": "AS", "orcid": "0000-0002-2696-5850", "researcher": {"href": "https://publications.scilifelab.se/researcher/909bca2fc68645e980a93b99dc150e4c.json"}}], "type": "journal article", "published": "2026-04-15", "journal": {"title": "Sci Transl Med", "issn": "1946-6242", "volume": "18", "issue": "845", "pages": "eadw3590", "issn-l": "1946-6234"}, "abstract": "The cartilaginous growth plate is a critical organ responsible for longitudinal bone growth. It closes after puberty in humans but remains open throughout life in mice. Although cartilage stem cells have been identified in murine growth plates, their existence in humans and their regulation by growth hormone (GH), the most widely used therapy for growth retardation, remain unknown. Here, we characterized the cellular and molecular organization of early pubertal human growth plates using unique surgical specimens from growth-restricting procedures and examined their direct responsiveness to GH. Single-cell and spatial analyses revealed two distinct stemlike populations in the resting zone, differing in proliferative activity, molecular identity, and regulatory cues. The root stem cells express multiple skeletal stem cell markers but not parathyroid hormone-related peptide and reside in a specialized microenvironment low in WNT and TGF-\u03b2 growth factors. A similar population was identified in transcriptionally profiled unsorted murine growth plates, and clonal lineage tracing demonstrated that these root cells, marked by expression of the Prrx1 gene, generate extensive chondrocyte clones and differentiate into stromal and osteoblastic lineages, confirming their stem cell properties. Human growth plate explant cultures showed that GH directly activates JAK/STAT, TGF-\u03b2, and ERK intracellular signaling pathways, inhibits AKT signaling, and stimulates cartilage growth and proliferation of cartilage stem cells and chondrocytes in the proliferative zone. Together, these findings uncover a conserved dual stem cell organization in human and mouse growth plates and define direct mechanisms of GH action, providing a framework for optimizing growth-promoting therapies.", "doi": "10.1126/scitranslmed.adw3590", "pmid": "41984930", "labels": {"NGI Short read": "Service", "NGI Stockholm (Genomics Production)": "Service", "National Genomics Infrastructure": "Service"}, "xrefs": [], "notes": [], "created": "2026-05-11T12:02:43.939Z", "modified": "2026-05-11T12:02:44.448Z"}, {"entity": "publication", "iuid": "85067a3ac4764a808733b9d4d4adc266", "links": {"self": {"href": "https://publications.scilifelab.se/publication/85067a3ac4764a808733b9d4d4adc266.json"}, "display": {"href": "https://publications.scilifelab.se/publication/85067a3ac4764a808733b9d4d4adc266"}}, "title": "Testosterone exacerbates neutrophilia and cardiac injury in myocardial infarction via actions in bone marrow.", "authors": [{"family": "Svedlund Eriksson", "given": "Elin", "initials": "E", "orcid": "0009-0006-0169-6929", "researcher": {"href": "https://publications.scilifelab.se/researcher/afb8b198881944a28e575e8ca8a2cc1d.json"}}, {"family": "Lantero Rodriguez", "given": "Marta", "initials": "M", "orcid": "0000-0002-9912-6716", "researcher": {"href": "https://publications.scilifelab.se/researcher/bee7c52fd83149fbbb01ced1cf5fa79d.json"}}, {"family": "Halvorsen", "given": "Bente", "initials": "B", "orcid": "0000-0002-6529-6485", "researcher": {"href": "https://publications.scilifelab.se/researcher/83218278a90c41609ea44fcd6315d395.json"}}, {"family": "Johansson", "given": "Inger", "initials": "I", "orcid": "0009-0008-3579-9031", "researcher": {"href": "https://publications.scilifelab.se/researcher/bbc07dcc5a824676a0f788ebe060ca02.json"}}, {"family": "M\u00e5rtensson", "given": "Anna K F", "initials": "AKF", "orcid": "0000-0002-8786-4965", "researcher": {"href": "https://publications.scilifelab.se/researcher/13ca4b04916d4318b8e00f1f5f4ca6d3.json"}}, {"family": "Wilhelmson", "given": "Anna S", "initials": "AS", "orcid": "0000-0003-0640-0251", "researcher": {"href": "https://publications.scilifelab.se/researcher/73ed1ad8dfa2406081cef9eb005b3ad6.json"}}, {"family": "Huse", "given": "Camilla", "initials": "C", "orcid": "0000-0002-7694-5196", "researcher": {"href": "https://publications.scilifelab.se/researcher/1025010995224e44bae6f9c15813de38.json"}}, {"family": "Ueland", "given": "Thor", "initials": "T"}, {"family": "Aukrust", "given": "P\u00e5l", "initials": "P", "orcid": "0000-0003-0919-836X", "researcher": {"href": "https://publications.scilifelab.se/researcher/b52ec6832f0742b4be1f6dd2e455a1ed.json"}}, {"family": "Broch", "given": "Kaspar", "initials": "K", "orcid": "0000-0003-4316-805X", "researcher": {"href": "https://publications.scilifelab.se/researcher/8c4d9562e7294f849d3ff4508d353bf9.json"}}, {"family": "Gullestad", "given": "Lars", "initials": "L"}, {"family": "Amundsen", "given": "Brage H\u00f8yem", "initials": "BH", "orcid": "0000-0001-7914-7395", "researcher": {"href": "https://publications.scilifelab.se/researcher/b7ee62e8f93047258965bcf42bb4a475.json"}}, {"family": "Andersen", "given": "Geir \u00d8ystein", "initials": "G\u00d8", "orcid": "0000-0003-1578-6440", "researcher": {"href": "https://publications.scilifelab.se/researcher/a498990cfa894f74bd3a273a1bbaee58.json"}}, {"family": "Karlsson", "given": "Mikael C I", "initials": "MCI", "orcid": "0000-0001-5582-614X", "researcher": {"href": "https://publications.scilifelab.se/researcher/9b7a0612c55f4ab8865b6e5f59347271.json"}}, {"family": "Hagberg Thulin", "given": "Malin", "initials": "M", "orcid": "0000-0002-8673-1247", "researcher": {"href": "https://publications.scilifelab.se/researcher/fbf6e0d2d6f748e39f32a70e1e4d597f.json"}}, {"family": "Camponeschi", "given": "Alessandro", "initials": "A", "orcid": "0000-0002-6472-2438", "researcher": {"href": "https://publications.scilifelab.se/researcher/ccbaffafd6a24f4abed1e402219fa472.json"}}, {"family": "Trompet", "given": "Dana", "initials": "D", "orcid": "0000-0001-9472-6184", "researcher": {"href": "https://publications.scilifelab.se/researcher/3ef4d02ec2e1427088522df2a759bf3a.json"}}, {"family": "Hammarsten", "given": "Ola", "initials": "O"}, {"family": "Redfors", "given": "Bj\u00f6rn", "initials": "B"}, {"family": "Bor\u00e9n", "given": "Jan", "initials": "J"}, {"family": "Omerovic", "given": "Elmir", "initials": "E"}, {"family": "Levin", "given": "Malin C", "initials": "MC"}, {"family": "Chagin", "given": "Andrei S", "initials": "AS", "orcid": "0000-0002-2696-5850", "researcher": {"href": "https://publications.scilifelab.se/researcher/909bca2fc68645e980a93b99dc150e4c.json"}}, {"family": "Dahl", "given": "Tuva B", "initials": "TB", "orcid": "0000-0001-7818-9411", "researcher": {"href": "https://publications.scilifelab.se/researcher/ff93848d0aac4a9fbf8ec2bbedebadea.json"}}, {"family": "Tivesten", "given": "\u00c5sa", "initials": "\u00c5", "orcid": "0000-0002-8318-0486", "researcher": {"href": "https://publications.scilifelab.se/researcher/55ade4099dfd4859970a6fe21af50870.json"}}], "type": "journal article", "published": "2025-02-05", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "16", "issue": "1", "pages": "1142", "issn-l": "2041-1723"}, "abstract": "Men develop larger infarct sizes than women after a myocardial infarction (MI), but the mechanism underlying this sex difference is unknown. Here, we demonstrated that blood neutrophil counts post-MI were higher in male than female mice. Castration-induced testosterone deficiency reduced blood neutrophil counts to the level in females and increased survival post-MI. These effects were mimicked by Osterix-directed ablation of the androgen receptor in bone marrow (BM). Mechanistically, androgens downregulated the leukocyte retention factor CXCL12 in BM stromal cells. Post-hoc analysis of clinical trial data showed that neutrophilia was greater in men than women after reperfusion of first-time ST-elevation MI, and tocilizumab, an interleukin-6 receptor inhibitor, reduced blood neutrophil counts and infarct size to a greater extent in men than women. Our work reveals a previously unknown mechanism connecting testosterone with neutrophilia and MI injury via BM and identifies the importance of considering sex when developing anti-inflammatory strategies to treat MI.", "doi": "10.1038/s41467-025-56217-x", "pmid": "39910039", "labels": {"Clinical Genomics": "Service", "Clinical Genomics Gothenburg": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC11799197"}, {"db": "pii", "key": "10.1038/s41467-025-56217-x"}], "notes": [], "created": "2025-07-08T13:56:49.778Z", "modified": "2025-11-04T11:38:37.313Z"}]}