{"entity": "researcher", "timestamp": "2026-07-17T07:46:41.220Z", "family": "Mold", "given": "Jeff E", "initials": "JE", "orcid": "0000-0003-2195-2978", "affiliations": ["Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/3ba167f912244d879746ed60a3c19568.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/3ba167f912244d879746ed60a3c19568"}}, "publications": [{"entity": "publication", "iuid": "aa7e2fd0de904b36aabb562ce819de11", "links": {"self": {"href": "https://publications.scilifelab.se/publication/aa7e2fd0de904b36aabb562ce819de11.json"}, "display": {"href": "https://publications.scilifelab.se/publication/aa7e2fd0de904b36aabb562ce819de11"}}, "title": "Spatial transcriptomics of B cell and T cell receptors reveals lymphocyte clonal dynamics.", "authors": [{"family": "Engblom", "given": "Camilla", "initials": "C", "orcid": "0000-0001-5090-4161", "researcher": {"href": "https://publications.scilifelab.se/researcher/5ae4350efff0421393356f3ff1f2a971.json"}}, {"family": "Thrane", "given": "Kim", "initials": "K", "orcid": "0000-0003-3109-5551", "researcher": {"href": "https://publications.scilifelab.se/researcher/f1bd1b94e1694de9a5c27fd8f331dc86.json"}}, {"family": "Lin", "given": "Qirong", "initials": "Q", "orcid": "0000-0001-5298-7861", "researcher": {"href": "https://publications.scilifelab.se/researcher/d10a182a9f0a4ec68ff809d1ac5a13a0.json"}}, {"family": "Andersson", "given": "Alma", "initials": "A", "orcid": "0000-0002-4773-9975", "researcher": {"href": "https://publications.scilifelab.se/researcher/7e483b7a04bd44d0ad31e15569ea7ea9.json"}}, {"family": "Toosi", "given": "Hosein", "initials": "H", "orcid": "0000-0002-5525-4724", "researcher": {"href": "https://publications.scilifelab.se/researcher/1024be99666a46cd97c75f9220f5b931.json"}}, {"family": "Chen", "given": "Xinsong", "initials": "X", "orcid": "0000-0002-3214-9075", "researcher": {"href": "https://publications.scilifelab.se/researcher/561d04f60c61426bb790ba83153ba651.json"}}, {"family": "Steiner", "given": "Embla", "initials": "E"}, {"family": "Lu", "given": "Chang", "initials": "C", "orcid": "0000-0001-6304-2820", "researcher": {"href": "https://publications.scilifelab.se/researcher/b0204945479c4066896ba3643fa4229a.json"}}, {"family": "Mantovani", "given": "Giulia", "initials": "G", "orcid": "0000-0002-5307-165X", "researcher": {"href": "https://publications.scilifelab.se/researcher/01f8e781842747bb91bdb07e9d5c0cd8.json"}}, {"family": "Hagemann-Jensen", "given": "Michael", "initials": "M", "orcid": "0000-0002-6423-8216", "researcher": {"href": "https://publications.scilifelab.se/researcher/26cb45960bd042c498f4914a342312a0.json"}}, {"family": "Saarenp\u00e4\u00e4", "given": "Sami", "initials": "S", "orcid": "0000-0003-4731-6857", "researcher": {"href": "https://publications.scilifelab.se/researcher/ee6979cdfc0b4e4285f9d810c39bb7b7.json"}}, {"family": "Jangard", "given": "Mattias", "initials": "M", "orcid": "0000-0003-3772-2026", "researcher": {"href": "https://publications.scilifelab.se/researcher/7dafcc3cdede495aabccee445306fac1.json"}}, {"family": "Saez-Rodriguez", "given": "Julio", "initials": "J", "orcid": "0000-0002-8552-8976", "researcher": {"href": "https://publications.scilifelab.se/researcher/ce0043f77cad4f6e8ab9641d7f42c420.json"}}, {"family": "Micha\u00eblsson", "given": "Jakob", "initials": "J"}, {"family": "Hartman", "given": "Johan", "initials": "J", "orcid": "0000-0002-6500-8527", "researcher": {"href": "https://publications.scilifelab.se/researcher/da7cefda6e00463d8ba95fc63eeb8f0a.json"}}, {"family": "Lagergren", "given": "Jens", "initials": "J", "orcid": "0000-0002-4552-0240", "researcher": {"href": "https://publications.scilifelab.se/researcher/b956941833b843f6ace483bf4c21e643.json"}}, {"family": "Mold", "given": "Jeff E", "initials": "JE", "orcid": "0000-0003-2195-2978", "researcher": {"href": "https://publications.scilifelab.se/researcher/3ba167f912244d879746ed60a3c19568.json"}}, {"family": "Lundeberg", "given": "Joakim", "initials": "J", "orcid": "0000-0003-4313-1601", "researcher": {"href": "https://publications.scilifelab.se/researcher/4a4e6ca0f29b4ead8569e2729481c3e0.json"}}, {"family": "Fris\u00e9n", "given": "Jonas", "initials": "J", "orcid": "0000-0001-5819-458X", "researcher": {"href": "https://publications.scilifelab.se/researcher/23064ee2ac9b4c2fb1eb94e61f92148e.json"}}], "type": "journal article", "published": "2023-12-08", "journal": {"title": "Science", "issn": "1095-9203", "issn-l": "0036-8075", "volume": "382", "issue": "6675", "pages": "eadf8486"}, "abstract": "The spatial distribution of lymphocyte clones within tissues is critical to their development, selection, and expansion. We have developed spatial transcriptomics of variable, diversity, and joining (VDJ) sequences (Spatial VDJ), a method that maps B cell and T cell receptor sequences in human tissue sections. Spatial VDJ captures lymphocyte clones that match canonical B and T cell distributions and amplifies clonal sequences confirmed by orthogonal methods. We found spatial congruency between paired receptor chains, developed a computational framework to predict receptor pairs, and linked the expansion of distinct B cell clones to different tumor-associated gene expression programs. Spatial VDJ delineates B cell clonal diversity and lineage trajectories within their anatomical niche. Thus, Spatial VDJ captures lymphocyte spatial clonal architecture across tissues, providing a platform to harness clonal sequences for therapy.", "doi": "10.1126/science.adf8486", "pmid": "38060664", "labels": {"NGI Uppsala (Uppsala Genome Center)": "Service", "NGI Long read": "Service", "National Genomics Infrastructure": "Service", "NGI Short read": null, "NGI Stockholm (Genomics Production)": null, "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [], "notes": [], "created": "2023-12-09T11:23:39.309Z", "modified": "2024-01-16T13:48:31.521Z"}, {"entity": "publication", "iuid": "f5829319dbfb49ad8137f41b82951481", "links": {"self": {"href": "https://publications.scilifelab.se/publication/f5829319dbfb49ad8137f41b82951481.json"}, "display": {"href": "https://publications.scilifelab.se/publication/f5829319dbfb49ad8137f41b82951481"}}, "title": "Prostate cancer disease recurrence after radical prostatectomy is associated with HLA type and local cytomegalovirus immunity.", "authors": [{"family": "Classon", "given": "Johanna", "initials": "J", "orcid": "0000-0003-0392-7605", "researcher": {"href": "https://publications.scilifelab.se/researcher/641cf9db52e64f9fb8eee60a977a88be.json"}}, {"family": "Zamboni", "given": "Margherita", "initials": "M", "orcid": "0000-0003-0664-4707", "researcher": {"href": "https://publications.scilifelab.se/researcher/1f196173b40b4819a5fcd11bf76a4e6e.json"}}, {"family": "Engblom", "given": "Camilla", "initials": "C", "orcid": "0000-0001-5090-4161", "researcher": {"href": "https://publications.scilifelab.se/researcher/5ae4350efff0421393356f3ff1f2a971.json"}}, {"family": "Alkass", "given": "Kanar", "initials": "K"}, {"family": "Mantovani", "given": "Giulia", "initials": "G", "orcid": "0000-0002-5307-165X", "researcher": {"href": "https://publications.scilifelab.se/researcher/01f8e781842747bb91bdb07e9d5c0cd8.json"}}, {"family": "Pou", "given": "Christian", "initials": "C", "orcid": "0000-0003-3932-788X", "researcher": {"href": "https://publications.scilifelab.se/researcher/a6015314c4c04ee08d7e4358cfb55b9f.json"}}, {"family": "Nkulikiyimfura", "given": "Dieudonn\u00e9", "initials": "D", "orcid": "0000-0002-6981-2053", "researcher": {"href": "https://publications.scilifelab.se/researcher/6af6961b78de4d0b96b011009cccc14c.json"}}, {"family": "Brodin", "given": "Petter", "initials": "P", "orcid": "0000-0002-8103-0046", "researcher": {"href": "https://publications.scilifelab.se/researcher/40097353cdb24e52bf2330eb687042bf.json"}}, {"family": "Druid", "given": "Henrik", "initials": "H"}, {"family": "Mold", "given": "Jeff", "initials": "J", "orcid": "0000-0003-2195-2978", "researcher": {"href": "https://publications.scilifelab.se/researcher/3ba167f912244d879746ed60a3c19568.json"}}, {"family": "Fris\u00e9n", "given": "Jonas", "initials": "J", "orcid": "0000-0001-5819-458X", "researcher": {"href": "https://publications.scilifelab.se/researcher/23064ee2ac9b4c2fb1eb94e61f92148e.json"}}], "type": "journal article", "published": "2022-10-00", "journal": {"title": "Mol Oncol", "issn": "1878-0261", "volume": "16", "issue": "19", "pages": "3452-3464", "issn-l": "1574-7891"}, "abstract": "Prostate cancer is a heterogeneous disease with a need for new prognostic biomarkers. Human leukocyte antigen (HLA) genes are highly polymorphic genes central to antigen presentation to T-cells. Two alleles, HLA-A*02:01 and HLA-A*24:02, have been associated with prognosis in patients diagnosed with de novo metastatic prostate cancer. We leveraged the next-generation sequenced cohorts CPC-GENE and TCGA-PRAD to examine HLA alleles, antiviral T-cell receptors and prostate cancer disease recurrence after prostatectomy. Carrying HLA-A*02:01 (111/229; 48% of patients) was independently associated with disease recurrence in patients with low-intermediate risk prostate cancer. HLA-A*11 (carried by 42/441; 10% of patients) was independently associated with rapid disease recurrence in patients with high-risk prostate cancer. Moreover, HLA-A*02:01 carriers in which anti-cytomegalovirus T-cell receptors (CMV-TCR) were identified in tumors (13/144; 10% of all patients in the cohort) had a higher risk of disease recurrence than CMV-TCR-negative patients. These findings suggest that HLA-type and CMV immunity may be valuable biomarkers for prostate cancer progression.", "doi": "10.1002/1878-0261.13273", "pmid": "35712787", "labels": {"Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC9533687"}, {"db": "RefSeq", "key": "NC_006273.2"}], "notes": [], "created": "2022-11-09T15:51:53.564Z", "modified": "2024-01-16T13:48:34.865Z"}, {"entity": "publication", "iuid": "6ba3a0a503434b1d9709bdead1716425", "links": {"self": {"href": "https://publications.scilifelab.se/publication/6ba3a0a503434b1d9709bdead1716425.json"}, "display": {"href": "https://publications.scilifelab.se/publication/6ba3a0a503434b1d9709bdead1716425"}}, "title": "Expansions of adaptive-like NK cells with a tissue-resident phenotype in human lung and blood.", "authors": [{"family": "Brownlie", "given": "Demi", "initials": "D", "orcid": "0000-0001-5932-6425", "researcher": {"href": "https://publications.scilifelab.se/researcher/4e879a6ef8ff45bea049986707480e99.json"}}, {"family": "Scharenberg", "given": "Marlena", "initials": "M", "orcid": "0000-0002-7659-7216", "researcher": {"href": "https://publications.scilifelab.se/researcher/4ef22a304bdc429a81d7acf143f6295f.json"}}, {"family": "Mold", "given": "Jeff E", "initials": "JE", "orcid": "0000-0003-2195-2978", "researcher": {"href": "https://publications.scilifelab.se/researcher/3ba167f912244d879746ed60a3c19568.json"}}, {"family": "H\u00e5rd", "given": "Joanna", "initials": "J", "orcid": "0000-0003-4575-5629", "researcher": {"href": "https://publications.scilifelab.se/researcher/3c209a378be54ae699e7157892c3a709.json"}}, {"family": "Kek\u00e4l\u00e4inen", "given": "Eliisa", "initials": "E", "orcid": "0000-0001-6045-108X", "researcher": {"href": "https://publications.scilifelab.se/researcher/726903b084194aeb885b4cb42a8d33db.json"}}, {"family": "Buggert", "given": "Marcus", "initials": "M", "orcid": "0000-0003-0633-1719", "researcher": {"href": "https://publications.scilifelab.se/researcher/9a54e4b5136642eeafa77ac5118a0c81.json"}}, {"family": "Nguyen", "given": "Son", "initials": "S"}, {"family": "Wilson", "given": "Jennifer N", "initials": "JN"}, {"family": "Al-Ameri", "given": "Mamdoh", "initials": "M"}, {"family": "Ljunggren", "given": "Hans-Gustaf", "initials": "HG", "orcid": "0000-0003-0908-7387", "researcher": {"href": "https://publications.scilifelab.se/researcher/54d696167bca4a389807143f84baeafe.json"}}, {"family": "Marquardt", "given": "Nicole", "initials": "N", "orcid": "0000-0003-3186-4752", "researcher": {"href": "https://publications.scilifelab.se/researcher/b87ccad53d354bdd80af490dc8a0c019.json"}}, {"family": "Micha\u00eblsson", "given": "Jakob", "initials": "J"}], "type": "journal article", "published": "2021-03-16", "journal": {"title": "Proc. Natl. Acad. Sci. U.S.A.", "issn": "1091-6490", "volume": "118", "issue": "11", "pages": "e2016580118", "issn-l": "0027-8424"}, "abstract": "Human adaptive-like \"memory\" CD56dimCD16+ natural killer (NK) cells in peripheral blood from cytomegalovirus-seropositive individuals have been extensively investigated in recent years and are currently explored as a treatment strategy for hematological cancers. However, treatment of solid tumors remains limited due to insufficient NK cell tumor infiltration, and it is unknown whether large expansions of adaptive-like NK cells that are equipped for tissue residency and tumor homing exist in peripheral tissues. Here, we show that human lung and blood contains adaptive-like CD56brightCD16- NK cells with hallmarks of tissue residency, including expression of CD49a. Expansions of adaptive-like lung tissue-resident NK (trNK) cells were found to be present independently of adaptive-like CD56dimCD16+ NK cells and to be hyperresponsive toward target cells. Together, our data demonstrate that phenotypically, functionally, and developmentally distinct subsets of adaptive-like NK cells exist in human lung and blood. Given their tissue-related character and hyperresponsiveness, human lung adaptive-like trNK cells might represent a suitable alternative for therapies targeting solid tumors.", "doi": "10.1073/pnas.2016580118", "pmid": "33836578", "labels": {"NGI Stockholm (Genomics Production)": null, "NGI Stockholm (Genomics Applications)": null, "National Genomics Infrastructure": null}, "xrefs": [{"db": "pii", "key": "2016580118"}, {"db": "pmc", "key": "PMC7980282"}], "notes": [], "created": "2021-06-09T12:16:15.739Z", "modified": "2021-11-10T12:26:18.872Z"}, {"entity": "publication", "iuid": "890d7e3c8843489cb12c5348644a4b42", "links": {"self": {"href": "https://publications.scilifelab.se/publication/890d7e3c8843489cb12c5348644a4b42.json"}, "display": {"href": "https://publications.scilifelab.se/publication/890d7e3c8843489cb12c5348644a4b42"}}, "title": "Cell generation dynamics underlying naive T-cell homeostasis in adult humans.", "authors": [{"family": "Mold", "given": "Jeff E", "initials": "JE", "orcid": "0000-0003-2195-2978", "researcher": {"href": "https://publications.scilifelab.se/researcher/3ba167f912244d879746ed60a3c19568.json"}}, {"family": "R\u00e9u", "given": "Pedro", "initials": "P"}, {"family": "Olin", "given": "Axel", "initials": "A", "orcid": "0000-0002-1161-4476", "researcher": {"href": "https://publications.scilifelab.se/researcher/8dfb8efde80a4a508c93714729259aba.json"}}, {"family": "Bernard", "given": "Samuel", "initials": "S", "orcid": "0000-0002-8442-9968", "researcher": {"href": "https://publications.scilifelab.se/researcher/dcf40de611c54f8ab7235b728b1b3c08.json"}}, {"family": "Micha\u00eblsson", "given": "Jakob", "initials": "J"}, {"family": "Rane", "given": "Sanket", "initials": "S"}, {"family": "Yates", "given": "Andrew", "initials": "A", "orcid": "0000-0003-4606-4483", "researcher": {"href": "https://publications.scilifelab.se/researcher/73cf7a0a7ebb4da39daced33ae3a6281.json"}}, {"family": "Khosravi", "given": "Azadeh", "initials": "A"}, {"family": "Salehpour", "given": "Mehran", "initials": "M"}, {"family": "Possnert", "given": "G\u00f6ran", "initials": "G", "orcid": "0000-0002-4840-291X", "researcher": {"href": "https://publications.scilifelab.se/researcher/bd2241f59305474b83489bd1d7a29618.json"}}, {"family": "Brodin", "given": "Petter", "initials": "P", "orcid": "0000-0002-8103-0046", "researcher": {"href": "https://publications.scilifelab.se/researcher/40097353cdb24e52bf2330eb687042bf.json"}}, {"family": "Fris\u00e9n", "given": "Jonas", "initials": "J", "orcid": "0000-0001-5819-458X", "researcher": {"href": "https://publications.scilifelab.se/researcher/23064ee2ac9b4c2fb1eb94e61f92148e.json"}}], "type": "journal article", "published": "2019-10-00", "journal": {"volume": "17", "issn": "1545-7885", "issue": "10", "pages": "e3000383", "title": "PLoS Biol.", "issn-l": "1544-9173"}, "abstract": "Thymic involution and proliferation of naive T cells both contribute to shaping the naive T-cell repertoire as humans age, but a clear understanding of the roles of each throughout a human life span has been difficult to determine. By measuring nuclear bomb test-derived 14C in genomic DNA, we determined the turnover rates of CD4+ and CD8+ naive T-cell populations and defined their dynamics in healthy individuals ranging from 20 to 65 years of age. We demonstrate that naive T-cell generation decreases with age because of a combination of declining peripheral division and thymic production during adulthood. Concomitant decline in T-cell loss compensates for decreased generation rates. We investigated putative mechanisms underlying age-related changes in homeostatic regulation of CD4+ naive T-cell turnover, using mass cytometry to profile candidate signaling pathways involved in T-cell activation and proliferation relative to CD31 expression, a marker of thymic proximity for the CD4+ naive T-cell population. We show that basal nuclear factor \u03baB (NF-\u03baB) phosphorylation positively correlated with CD31 expression and thus is decreased in peripherally expanded naive T-cell clones. Functionally, we found that NF-\u03baB signaling was essential for naive T-cell proliferation to the homeostatic growth factor interleukin (IL)-7, and reduced NF-\u03baB phosphorylation in CD4+CD31- naive T cells is linked to reduced homeostatic proliferation potential. Our results reveal an age-related decline in naive T-cell turnover as a putative regulator of naive T-cell diversity and identify a molecular pathway that restricts proliferation of peripherally expanded naive T-cell clones that accumulate with age.", "doi": "10.1371/journal.pbio.3000383", "pmid": "31661488", "labels": {"Cellular Immunomonitoring": "Collaborative", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pii", "key": "PBIOLOGY-D-19-01355"}, {"db": "pmc", "key": "PMC6818757"}], "notes": [], "created": "2019-10-30T18:30:18.814Z", "modified": "2024-01-16T13:48:43.790Z"}]}