{"entity": "researcher", "timestamp": "2026-06-17T19:16:20.329Z", "family": "L\u00fcnsdorf", "given": "Heinrich", "initials": "H", "orcid": "0000-0003-2778-8611", "affiliations": ["Helmholtz Centre for Infection Research Braunschweig Germany"], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/35509ad2567340b1b6fbc1355ba67db7.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/35509ad2567340b1b6fbc1355ba67db7"}}, "publications": [{"entity": "publication", "iuid": "8900950a1d67461cb627e4a31b553d07", "links": {"self": {"href": "https://publications.scilifelab.se/publication/8900950a1d67461cb627e4a31b553d07.json"}, "display": {"href": "https://publications.scilifelab.se/publication/8900950a1d67461cb627e4a31b553d07"}}, "title": "A recently isolated human commensal Escherichia coli ST10 clone member mediates enhanced thermotolerance and tetrathionate respiration on a P1 phage-derived IncY plasmid.", "authors": [{"family": "Kamal", "given": "Shady Mansour", "initials": "SM", "orcid": "0000-0002-7565-811X", "researcher": {"href": "https://publications.scilifelab.se/researcher/5ef64c5ead434175a8ebaf26a786a916.json"}}, {"family": "Cimdins-Ahne", "given": "Annika", "initials": "A", "orcid": "0000-0001-7414-9914", "researcher": {"href": "https://publications.scilifelab.se/researcher/e45bd6f6716e442aa9800ade4b2c9ccb.json"}}, {"family": "Lee", "given": "Changhan", "initials": "C", "orcid": "0000-0003-0327-4712", "researcher": {"href": "https://publications.scilifelab.se/researcher/1c2596edd9c74f0abcdeea30a0c1a1dd.json"}}, {"family": "Li", "given": "Fengyang", "initials": "F", "orcid": "0000-0001-5102-6284", "researcher": {"href": "https://publications.scilifelab.se/researcher/9df6d813f9c846288355fe6695f7e4ab.json"}}, {"family": "Mart\u00edn-Rodr\u00edguez", "given": "Alberto J", "initials": "AJ", "orcid": "0000-0003-2422-129X", "researcher": {"href": "https://publications.scilifelab.se/researcher/65ffdf18851a414798718b1e349e41be.json"}}, {"family": "Seferbekova", "given": "Zaira", "initials": "Z", "orcid": "0000-0002-3131-3697", "researcher": {"href": "https://publications.scilifelab.se/researcher/b880e4cdf6594edab82e28ae3739a77d.json"}}, {"family": "Afasizhev", "given": "Robert", "initials": "R", "orcid": "0000-0002-3513-8873", "researcher": {"href": "https://publications.scilifelab.se/researcher/e21c2af9f4b8403795927dfa4287f2b4.json"}}, {"family": "Wami", "given": "Haleluya Tesfaye", "initials": "HT", "orcid": "0000-0002-9929-2570", "researcher": {"href": "https://publications.scilifelab.se/researcher/89117b1a4ee04e65b405bb13697b6b06.json"}}, {"family": "Katikaridis", "given": "Panagiotis", "initials": "P", "orcid": "0000-0001-5783-1487", "researcher": {"href": "https://publications.scilifelab.se/researcher/b6ba96692acd4b2b8412eca76ed1f1a9.json"}}, {"family": "Meins", "given": "Lena", "initials": "L", "orcid": "0000-0001-6214-477X", "researcher": {"href": "https://publications.scilifelab.se/researcher/3cb7a68bdfc34a508235b978d4b59249.json"}}, {"family": "L\u00fcnsdorf", "given": "Heinrich", "initials": "H", "orcid": "0000-0003-2778-8611", "researcher": {"href": "https://publications.scilifelab.se/researcher/35509ad2567340b1b6fbc1355ba67db7.json"}}, {"family": "Dobrindt", "given": "Ulrich", "initials": "U", "orcid": "0000-0001-9949-1898", "researcher": {"href": "https://publications.scilifelab.se/researcher/220a330bb66f48efaffd38d0efaa0f9e.json"}}, {"family": "Mogk", "given": "Axel", "initials": "A", "orcid": "0000-0003-3674-5410", "researcher": {"href": "https://publications.scilifelab.se/researcher/56a1d7fc519743029c9ae615086d8093.json"}}, {"family": "R\u00f6mling", "given": "Ute", "initials": "U", "orcid": "0000-0003-3812-6621", "researcher": {"href": "https://publications.scilifelab.se/researcher/7a59ac735d61485aa43ee7a2ae3a526d.json"}}], "type": "journal article", "published": "2021-02-00", "journal": {"title": "Mol. Microbiol.", "issn": "1365-2958", "volume": "115", "issue": "2", "pages": "255-271", "issn-l": "0950-382X"}, "abstract": "The ubiquitous human commensal Escherichia coli has been well investigated through its model representative E. coli K-12. In this work, we initially characterized E. coli Fec10, a recently isolated human commensal strain of phylogroup A/sequence type ST10. Compared to E. coli K-12, the 4.88 Mbp Fec10 genome is characterized by distinct single-nucleotide polymorphisms and acquisition of genomic islands. In addition, E. coli Fec10 possesses a 155.86 kbp IncY plasmid, a composite element based on phage P1. pFec10 harbours multiple cargo genes such as coding for a tetrathionate reductase and its corresponding regulatory two-component system. Among the cargo genes is also the Transmissible Locus of Protein Quality Control (TLPQC), which mediates tolerance to lethal temperatures in bacteria. The disaggregase ClpGGI of TLPQC constitutes a major determinant of the thermotolerance of E. coli Fec10. We confirmed stand-alone disaggregation activity, but observed distinct biochemical characteristics of ClpGGI-Fec10 compared to the nearly identical Pseudomonas aeruginosa ClpGGI-SG17M. Furthermore, we noted a unique contribution of ClpGGI-Fec10 to the exquisite thermotolerance of E. coli Fec10, suggesting functional differences between both disaggregases in vivo. Detection of thermotolerance in 10% of human commensal E. coli isolates hints to the successful establishment of food-borne heat-resistant strains in the human gut.", "doi": "10.1111/mmi.14614", "pmid": "32985020", "labels": {"NGI Stockholm (Genomics Production)": "Service", "National Genomics Infrastructure": "Service", "NGI Stockholm (Genomics Applications)": "Service", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC7984374"}], "notes": [], "created": "2020-12-07T16:29:51.854Z", "modified": "2024-01-16T13:48:40.873Z"}]}