{"entity": "researcher", "timestamp": "2026-09-22T22:02:11.269Z", "family": "Jose", "given": "Jeny", "initials": "J", "orcid": "0000-0002-4518-3483", "affiliations": ["Ume\u00e5 Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, 90183 Ume\u00e5, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/32fe4331e8ad465185c0989f26d80b1c.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/32fe4331e8ad465185c0989f26d80b1c"}}, "publications": [{"entity": "publication", "iuid": "e979f660e928462caa9622ee83ce89ef", "links": {"self": {"href": "https://publications.scilifelab.se/publication/e979f660e928462caa9622ee83ce89ef.json"}, "display": {"href": "https://publications.scilifelab.se/publication/e979f660e928462caa9622ee83ce89ef"}}, "title": "Nitrate transiently rewires transcription in developing xylem of Populus wood to promote cell expansion via CRF4.", "authors": [{"family": "Choudhary", "given": "Shruti", "initials": "S", "orcid": "0000-0002-6146-7224", "researcher": {"href": "https://publications.scilifelab.se/researcher/b6ebad9d4ddf40949a19776df31a00c7.json"}}, {"family": "Renstr\u00f6m", "given": "Anna", "initials": "A"}, {"family": "Kochakarn", "given": "Theerarat Fai", "initials": "TF", "orcid": "0000-0002-3524-8079", "researcher": {"href": "https://publications.scilifelab.se/researcher/f2501f4110824a9189ad1fd6ed9d732b.json"}}, {"family": "Miskolczi", "given": "Pal", "initials": "P", "orcid": "0000-0003-1036-5831", "researcher": {"href": "https://publications.scilifelab.se/researcher/3996e8f2de5d45df80f7559bdc9a5ce7.json"}}, {"family": "Piombo", "given": "Edoardo", "initials": "E", "orcid": "0000-0003-2830-1967", "researcher": {"href": "https://publications.scilifelab.se/researcher/4ff26b84d14643f3ae4e45a23cd2107b.json"}}, {"family": "Chantreau", "given": "Maxime", "initials": "M", "orcid": "0000-0002-2844-1989", "researcher": {"href": "https://publications.scilifelab.se/researcher/99982bf106fe4091a5007707624f7ed3.json"}}, {"family": "Jose", "given": "Jeny", "initials": "J", "orcid": "0000-0002-4518-3483", "researcher": {"href": "https://publications.scilifelab.se/researcher/32fe4331e8ad465185c0989f26d80b1c.json"}}, {"family": "J\u00e4mtg\u00e5rd", "given": "Sandra", "initials": "S", "orcid": "0000-0002-5222-7878", "researcher": {"href": "https://publications.scilifelab.se/researcher/84162671e5bf4570b9c6b4eec94e96b5.json"}}, {"family": "Gorzs\u00e1s", "given": "Andr\u00e1s", "initials": "A", "orcid": "0000-0002-2298-8844", "researcher": {"href": "https://publications.scilifelab.se/researcher/8070792ccead4c809c89943d84cf0e03.json"}}, {"family": "Henriksson", "given": "Johan", "initials": "J", "orcid": "0000-0002-7745-2844", "researcher": {"href": "https://publications.scilifelab.se/researcher/44339821900646b3881d4b4dfd09e8d5.json"}}, {"family": "Tuominen", "given": "Hannele", "initials": "H", "orcid": "0000-0002-4949-3702", "researcher": {"href": "https://publications.scilifelab.se/researcher/0fe575e1cb054ab08b57e05d3a1ee28d.json"}}], "type": "journal article", "published": "2026-09-18", "journal": {"title": "Plant Cell", "issn": "1532-298X", "issn-l": "1040-4651"}, "abstract": "Nitrate functions both as a nutrient and a signaling molecule, but how nitrate is perceived and translated into developmental outputs within woody tissues is unknown. Here, we show that developing xylem in hybrid aspen (Populus tremula \u00d7 P. tremuloides) mounts a rapid, cell-type-specific transcriptional response to nitrate that promotes xylem cell expansion. To isolate local nitrate signaling in wood-forming tissues, we delivered nitrate directly into the xylem sap and profiled transcriptomic responses from 2 to 48 h after treatment. Bulk RNA sequencing revealed a transient response peaking within 2-4 h. Single-cell RNA sequencing at the peak response resolved nitrate signaling across developing xylem cell types and showed that nitrate induced cell-type-specific programs linked to nitrogen transport, cell wall remodeling, and developmental progression, while repressing lignin-biosynthetic genes. These data indicate that nitrate acts transiently in wood-forming tissues to fine-tune early xylem development. Among nitrate-responsive transcription factors, CYTOKININ RESPONSE FACTOR 4 (CRF4) emerged as a candidate regulator of this response. CRF4 overexpression in hybrid aspen stimulated xylem cell expansion, whereas simultaneous knock-out of CRF4 and its paralog, CRF3, significantly attenuated the nitrate-induced expansion. Together, our findings identify a local nitrate signaling module in developing wood and establish CRF4 as a component linking nitrate sensing to xylem cell expansion.", "doi": "10.1093/plcell/koag281", "pmid": "42759494", "labels": {"NGI Stockholm (Genomics Production)": "Service", "National Genomics Infrastructure": "Service", "NGI Short read": "Service"}, "xrefs": [{"db": "pii", "key": "8817497"}], "notes": [], "created": "2026-09-22T13:30:58.917Z", "modified": "2026-09-22T13:31:00.464Z"}]}