{"entity": "researcher", "timestamp": "2026-10-12T03:00:49.610Z", "family": "Romani Vestman", "given": "Nelly", "initials": "N", "orcid": "0000-0002-5674-8179", "affiliations": [], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/3273e2c1a9cc4af699a0c61d2b52077a.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/3273e2c1a9cc4af699a0c61d2b52077a"}}, "publications": [{"entity": "publication", "iuid": "370814a7c3394127aa1626b532df0e55", "links": {"self": {"href": "https://publications.scilifelab.se/publication/370814a7c3394127aa1626b532df0e55.json"}, "display": {"href": "https://publications.scilifelab.se/publication/370814a7c3394127aa1626b532df0e55"}}, "title": "Combined Transcriptomic and Protein Array Cytokine Profiling of Human Stem Cells from Dental Apical Papilla Modulated by Oral Bacteria.", "authors": [{"family": "Zymovets", "given": "Valeriia", "initials": "V"}, {"family": "Razghonova", "given": "Yelyzaveta", "initials": "Y"}, {"family": "Rakhimova", "given": "Olena", "initials": "O", "orcid": "0000-0002-3536-4467", "researcher": {"href": "https://publications.scilifelab.se/researcher/727f418f4ea24859ba4bf0e4bdf1f64c.json"}}, {"family": "Aripaka", "given": "Karthik", "initials": "K", "orcid": "0000-0001-5071-6187", "researcher": {"href": "https://publications.scilifelab.se/researcher/2622fdbe964f4f99810cdc20c67a9fe0.json"}}, {"family": "Manoharan", "given": "Lokeshwaran", "initials": "L", "orcid": "0000-0001-9751-5745", "researcher": {"href": "https://publications.scilifelab.se/researcher/000321fd81b9457db66140246bbd9066.json"}}, {"family": "Kelk", "given": "Peyman", "initials": "P"}, {"family": "Landstr\u00f6m", "given": "Mar\u00e9ne", "initials": "M", "orcid": "0000-0001-6737-7230", "researcher": {"href": "https://publications.scilifelab.se/researcher/c2f02fcfb1c1497d81a6f343bc0e6928.json"}}, {"family": "Romani Vestman", "given": "Nelly", "initials": "N", "orcid": "0000-0002-5674-8179", "researcher": {"href": "https://publications.scilifelab.se/researcher/3273e2c1a9cc4af699a0c61d2b52077a.json"}}], "type": "journal article", "published": "2022-05-03", "journal": {"title": "Int J Mol Sci", "issn": "1422-0067", "volume": "23", "issue": "9", "pages": "5098", "issn-l": null}, "abstract": "Stem cells from the apical papilla (SCAP) are a promising resource for use in regenerative endodontic treatment (RET) that may be adversely affected by oral bacteria, which in turn can exert an effect on the success of RET. Our work aims to study the cytokine profile of SCAP upon exposure to oral bacteria and their supernatants-Fusobacterium nucleatum and Enterococcus faecalis-as well as to establish their effect on the osteogenic and immunogenic potentials of SCAP. Further, we target the presence of key proteins of the Wnt/\u03b2-Catenin, TGF-\u03b2, and NF-\u03baB signaling pathways, which play a crucial role in adult osteogenic differentiation of mesenchymal stem cells, using the Western blot (WB) technique. The membrane-based sandwich immunoassay and transcriptomic analysis showed that, under the influence of F. nucleatum (both bacteria and supernatant), the production of pro-inflammatory cytokines IL-6, IL-8, and MCP-1 occurred, which was also confirmed at the mRNA level. Conversely, E. faecalis reduced the secretion of the aforementioned cytokines at both mRNA and protein levels. WB analysis showed that SCAP co-cultivation with E. faecalis led to a decrease in the level of the key proteins of the Wnt/\u03b2-Catenin and NF-\u03baB signaling pathways: \u03b2-Catenin (p = 0.0068 *), LRP-5 (p = 0.0059 **), and LRP-6 (p = 0.0329 *), as well as NF-kB (p = 0.0034 **) and TRAF6 (p = 0.0285 *). These results suggest that oral bacteria can up- and downregulate the immune and inflammatory responses of SCAP, as well as influence the osteogenic potential of SCAP, which may negatively regulate the success of RET.", "doi": "10.3390/ijms23095098", "pmid": "35563488", "labels": {"Bioinformatics Support, Infrastructure and Training": "Collaborative", "Bioinformatics Support and Infrastructure": "Collaborative", "Bioinformatics Support for Computational Resources": "Service", "Bioinformatics (NBIS)": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC9103834"}, {"db": "pii", "key": "ijms23095098"}], "notes": [], "created": "2022-05-09T09:39:42.630Z", "modified": "2024-01-16T13:48:36.757Z"}, {"entity": "publication", "iuid": "fead16a7ade749c8a101ff49f4a772b3", "links": {"self": {"href": "https://publications.scilifelab.se/publication/fead16a7ade749c8a101ff49f4a772b3.json"}, "display": {"href": "https://publications.scilifelab.se/publication/fead16a7ade749c8a101ff49f4a772b3"}}, "title": "New Insights into the Microbial Profiles of Infected Root Canals in Traumatized Teeth.", "authors": [{"family": "Manoharan", "given": "Lokeshwaran", "initials": "L", "orcid": "0000-0001-9751-5745", "researcher": {"href": "https://publications.scilifelab.se/researcher/000321fd81b9457db66140246bbd9066.json"}}, {"family": "Brundin", "given": "Malin", "initials": "M"}, {"family": "Rakhimova", "given": "Olena", "initials": "O"}, {"family": "Ch\u00e1vez de Paz", "given": "Luis", "initials": "L"}, {"family": "Romani Vestman", "given": "Nelly", "initials": "N", "orcid": "0000-0002-5674-8179", "researcher": {"href": "https://publications.scilifelab.se/researcher/3273e2c1a9cc4af699a0c61d2b52077a.json"}}], "type": "journal article", "published": "2020-11-28", "journal": {"title": "J Clin Med", "issn": "2077-0383", "volume": "9", "issue": "12", "pages": "3877", "issn-l": "2077-0383"}, "abstract": "Traumatic dental injuries in young individuals are often exposed to the invasion of oral microorganisms that leads to pulp necrosis. Infective necrosis in permanent teeth not-fully-developed causes aberrant root formation. Regeneration endodontic treatments (RETs) have shown promising results by promoting continued root development by stem cells. Critical to the success of RET is the thorough disinfection of the pulpal space. To establish effective antimicrobial protocols for root canal disinfection, the invading microorganisms need to be identified. In the present study, we use a combination of culture-based and high-throughput molecular sequencing techniques to investigate the microbial profiles from traumatized teeth (30 cases) and controls, i.e., teeth with pulp infections not caused by trauma (32 cases). Overall, a high microbial diversity in traumatized necrotic teeth was observed. Eubacterium yurii subsps. yurii and margaretiae, as well as key 'bridging oral species' F. nucleatum sp., Polymorphum and Corynebacterium matruchotti, were highly associated with traumatized teeth. The microbial compositions of traumatized teeth differed considerably from those of infected teeth not caused by trauma. Age and tooth position also influence microbial compositions. In conclusion, we show that the root canal microflora of traumatized teeth is highly diverse, and it differs from root canal infections not caused by trauma.", "doi": "10.3390/jcm9123877", "pmid": "33260621", "labels": {"Bioinformatics Support, Infrastructure and Training": "Collaborative", "Bioinformatics Support and Infrastructure": "Collaborative", "Bioinformatics Support for Computational Resources": "Service", "Bioinformatics (NBIS)": "Collaborative"}, "xrefs": [{"db": "pii", "key": "jcm9123877"}, {"db": "pmc", "key": "PMC7760719"}], "notes": [], "created": "2020-11-30T10:28:42.776Z", "modified": "2024-01-16T13:48:41.302Z"}]}