{"entity": "researcher", "timestamp": "2026-08-17T00:55:03.584Z", "family": "Weber", "given": "Florian", "initials": "F", "orcid": "0000-0002-8946-1377", "affiliations": [], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/2d4b9aaf4b7041cdbf1d99f3a0d833f2.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/2d4b9aaf4b7041cdbf1d99f3a0d833f2"}}, "publications": [{"entity": "publication", "iuid": "d2c5072fe8644c0b8b5297199b1fd0e5", "links": {"self": {"href": "https://publications.scilifelab.se/publication/d2c5072fe8644c0b8b5297199b1fd0e5.json"}, "display": {"href": "https://publications.scilifelab.se/publication/d2c5072fe8644c0b8b5297199b1fd0e5"}}, "title": "Measuring plasma membrane fluidity using confocal microscopy.", "authors": [{"family": "Carravilla", "given": "Pablo", "initials": "P", "orcid": "0000-0001-6592-7630", "researcher": {"href": "https://publications.scilifelab.se/researcher/b791a8068a724c179b731f0446b1737a.json"}}, {"family": "Andronico", "given": "Luca", "initials": "L"}, {"family": "Schlegel", "given": "Jan", "initials": "J", "orcid": "0000-0003-3159-8079", "researcher": {"href": "https://publications.scilifelab.se/researcher/c94351acc3934c3084f1ed0429a1879a.json"}}, {"family": "Urem", "given": "Yagmur B", "initials": "YB", "orcid": "0009-0008-5053-2191", "researcher": {"href": "https://publications.scilifelab.se/researcher/78c952b0962443199c9be0ce64d48e4e.json"}}, {"family": "Sjule", "given": "Ellen", "initials": "E", "orcid": "0000-0003-0166-2826", "researcher": {"href": "https://publications.scilifelab.se/researcher/4e0fd8926ef843a0a8db936df7209107.json"}}, {"family": "Ragaller", "given": "Franziska", "initials": "F", "orcid": "0000-0002-4148-262X", "researcher": {"href": "https://publications.scilifelab.se/researcher/9ead87aca17f476b9febf3f020567c4b.json"}}, {"family": "Weber", "given": "Florian", "initials": "F", "orcid": "0000-0002-8946-1377", "researcher": {"href": "https://publications.scilifelab.se/researcher/2d4b9aaf4b7041cdbf1d99f3a0d833f2.json"}}, {"family": "Gurdap", "given": "Cenk O", "initials": "CO", "orcid": "0000-0002-5327-2523", "researcher": {"href": "https://publications.scilifelab.se/researcher/9e3e5501e8d84f46884f1001367ab262.json"}}, {"family": "Ascioglu", "given": "Yavuz", "initials": "Y"}, {"family": "Sych", "given": "Taras", "initials": "T", "orcid": "0000-0002-7330-9063", "researcher": {"href": "https://publications.scilifelab.se/researcher/d104a7d8096b4a4ab2a9fe618489fc7e.json"}}, {"family": "Lorent", "given": "Joseph", "initials": "J"}, {"family": "Sezgin", "given": "Erdinc", "initials": "E", "orcid": "0000-0002-4915-388X", "researcher": {"href": "https://publications.scilifelab.se/researcher/34d3b05d68d64f698ff08dc655d2fe26.json"}}], "type": "journal article", "published": "2025-02-19", "journal": {"title": "Nat Protoc", "issn": "1750-2799", "issn-l": null}, "abstract": "Membrane fluidity is a crucial parameter for cellular physiology. Recent evidence suggests that fluidity varies between cell types and states and in diseases. As membrane fluidity has gradually become an important consideration in cell biology and biomedicine, it is essential to have reliable and quantitative ways to measure it in cells. In the past decade, there has been substantial progress both in chemical probes and in imaging tools to make membrane fluidity measurements easier and more reliable. We have recently established a robust pipeline, using confocal imaging and new environment-sensitive probes, that has been successfully used for several studies. Here we present our detailed protocol for membrane fluidity measurement, from labeling to imaging and image analysis. The protocol takes ~4 h and requires basic expertise in cell culture, wet lab and microscopy.", "doi": "10.1038/s41596-024-01122-8", "pmid": "39972239", "labels": {"Integrated Microscopy Technologies Stockholm": "Service"}, "xrefs": [{"db": "pii", "key": "10.1038/s41596-024-01122-8"}], "notes": [], "created": "2025-03-11T12:49:46.696Z", "modified": "2025-03-24T08:20:58.620Z"}, {"entity": "publication", "iuid": "929d37e62723414485ce037608f6d317", "links": {"self": {"href": "https://publications.scilifelab.se/publication/929d37e62723414485ce037608f6d317.json"}, "display": {"href": "https://publications.scilifelab.se/publication/929d37e62723414485ce037608f6d317"}}, "title": "High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler.", "authors": [{"family": "Sych", "given": "Taras", "initials": "T"}, {"family": "Schlegel", "given": "Jan", "initials": "J"}, {"family": "Barriga", "given": "Hanna M G", "initials": "HMG"}, {"family": "Ojansivu", "given": "Miina", "initials": "M"}, {"family": "Hanke", "given": "Leo", "initials": "L", "orcid": "0000-0001-5514-2418", "researcher": {"href": "https://publications.scilifelab.se/researcher/ece050a286f946f6807170cffc9320e7.json"}}, {"family": "Weber", "given": "Florian", "initials": "F", "orcid": "0000-0002-8946-1377", "researcher": {"href": "https://publications.scilifelab.se/researcher/2d4b9aaf4b7041cdbf1d99f3a0d833f2.json"}}, {"family": "Beklem Bostancioglu", "given": "R", "initials": "R", "orcid": "0000-0001-6755-6968", "researcher": {"href": "https://publications.scilifelab.se/researcher/8787ba29dcd94dcbad7ca15f09d6dbe6.json"}}, {"family": "Ezzat", "given": "Kariem", "initials": "K"}, {"family": "Stangl", "given": "Herbert", "initials": "H"}, {"family": "Plochberger", "given": "Birgit", "initials": "B", "orcid": "0000-0003-2733-9947", "researcher": {"href": "https://publications.scilifelab.se/researcher/0b13d4bbb5c74e16b63bad82ab64fec6.json"}}, {"family": "Laurencikiene", "given": "Jurga", "initials": "J"}, {"family": "El Andaloussi", "given": "Samir", "initials": "S", "orcid": "0000-0003-4468-9113", "researcher": {"href": "https://publications.scilifelab.se/researcher/bd1036a42043441da3e444f4eac58010.json"}}, {"family": "F\u00fcrth", "given": "Daniel", "initials": "D"}, {"family": "Stevens", "given": "Molly M", "initials": "MM", "orcid": "0000-0002-7335-266X", "researcher": {"href": "https://publications.scilifelab.se/researcher/93e770c382624ceda6ac06f7325bff50.json"}}, {"family": "Sezgin", "given": "Erdinc", "initials": "E", "orcid": "0000-0002-4915-388X", "researcher": {"href": "https://publications.scilifelab.se/researcher/34d3b05d68d64f698ff08dc655d2fe26.json"}}], "type": "journal article", "published": "2024-04-00", "journal": {"title": "Nat. Biotechnol.", "issn": "1546-1696", "volume": "42", "issue": "4", "pages": "587-590", "issn-l": "1087-0156"}, "abstract": "We introduce a method, single-particle profiler, that provides single-particle information on the content and biophysical properties of thousands of particles in the size range 5-200 nm. We use our single-particle profiler to measure the messenger RNA encapsulation efficiency of lipid nanoparticles, the viral binding efficiencies of different nanobodies, and the biophysical heterogeneity of liposomes, lipoproteins, exosomes and viruses.", "doi": "10.1038/s41587-023-01825-5", "pmid": "37308687", "labels": {"Integrated Microscopy Technologies Stockholm": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC11021190"}, {"db": "pii", "key": "10.1038/s41587-023-01825-5"}], "notes": [], "created": "2023-06-13T08:54:40.506Z", "modified": "2024-08-27T08:00:02.011Z"}]}