{"entity": "researcher", "timestamp": "2026-07-11T23:39:22.326Z", "family": "Garc\u00eda-Soriano", "given": "Daniela A", "initials": "DA", "orcid": "0000-0003-2849-8528", "affiliations": ["Department of Cell and Molecular Biology, Uppsala University, SciLifeLab Uppsala, Uppsala, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/2aeeed6de99647a1878352e0da2220af.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/2aeeed6de99647a1878352e0da2220af"}}, "publications": [{"entity": "publication", "iuid": "87a87989503945b7842ab3f695a22318", "links": {"self": {"href": "https://publications.scilifelab.se/publication/87a87989503945b7842ab3f695a22318.json"}, "display": {"href": "https://publications.scilifelab.se/publication/87a87989503945b7842ab3f695a22318"}}, "title": "Pooled optical screening in bacteria using chromosomally expressed barcodes.", "authors": [{"family": "Soares", "given": "Ruben R G", "initials": "RRG", "orcid": "0000-0001-5958-5232", "researcher": {"href": "https://publications.scilifelab.se/researcher/88ea8e0d81284c35a271f05187289390.json"}}, {"family": "Garc\u00eda-Soriano", "given": "Daniela A", "initials": "DA", "orcid": "0000-0003-2849-8528", "researcher": {"href": "https://publications.scilifelab.se/researcher/2aeeed6de99647a1878352e0da2220af.json"}}, {"family": "Larsson", "given": "Jimmy", "initials": "J"}, {"family": "Fange", "given": "David", "initials": "D", "orcid": "0009-0006-3981-7618", "researcher": {"href": "https://publications.scilifelab.se/researcher/195ab3e1f94e4ad39b4aa32da6b3e217.json"}}, {"family": "Schirman", "given": "Dvir", "initials": "D", "orcid": "0000-0001-5175-0176", "researcher": {"href": "https://publications.scilifelab.se/researcher/02550d8122ed486fb67691b456926aed.json"}}, {"family": "Grillo", "given": "Marco", "initials": "M", "orcid": "0000-0003-2155-0645", "researcher": {"href": "https://publications.scilifelab.se/researcher/bcd8bec6567444558acbcbd1b3d28f7a.json"}}, {"family": "Kn\u00f6ppel", "given": "Anna", "initials": "A"}, {"family": "Sen", "given": "Beer Chakra", "initials": "BC"}, {"family": "Svahn", "given": "Fabian", "initials": "F", "orcid": "0000-0002-5914-520X", "researcher": {"href": "https://publications.scilifelab.se/researcher/3f015b5855854d40b853c17a31d3d632.json"}}, {"family": "Zikrin", "given": "Spartak", "initials": "S", "orcid": "0000-0002-7802-8869", "researcher": {"href": "https://publications.scilifelab.se/researcher/8a82f27ea7bf43deaded33d359270820.json"}}, {"family": "Ratz", "given": "Michael", "initials": "M", "orcid": "0000-0002-9795-8033", "researcher": {"href": "https://publications.scilifelab.se/researcher/a481899ca58a467499f56af4feb5457c.json"}}, {"family": "Nilsson", "given": "Mats", "initials": "M", "orcid": "0000-0001-9985-0387", "researcher": {"href": "https://publications.scilifelab.se/researcher/197cf8ba83ba430f9712b2f4d94dc3e5.json"}}, {"family": "Elf", "given": "Johan", "initials": "J", "orcid": "0000-0001-5522-1810", "researcher": {"href": "https://publications.scilifelab.se/researcher/e8c05ef8453040a794cb9c716f0ef8d6.json"}}], "type": "journal article", "published": "2025-06-03", "journal": {"title": "Commun Biol", "issn": "2399-3642", "volume": "8", "issue": "1", "pages": "851", "issn-l": "2399-3642"}, "abstract": "Optical pooled screening is an important tool to study dynamic phenotypes for libraries of genetically engineered cells. However, the desired engineering often requires that the barcodes used for in situ genotyping are expressed from the chromosome. This has not previously been achieved in bacteria. Here we describe a method for in situ genotyping of libraries with genomic barcodes in Escherichia coli. The method is applied to measure the intracellular maturation time of 84 red fluorescent proteins.", "doi": "10.1038/s42003-025-08268-5", "pmid": "40461651", "labels": {"Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC12134211"}, {"db": "pii", "key": "10.1038/s42003-025-08268-5"}], "notes": [], "created": "2025-11-28T10:47:35.410Z", "modified": "2025-11-28T10:47:35.689Z"}]}