{"entity": "researcher", "timestamp": "2026-07-11T14:25:48.292Z", "family": "Lauschke", "given": "Volker M", "initials": "VM", "orcid": "0000-0002-1140-6204", "affiliations": ["Department of Physiology and PharmacologyKarolinska Institutet Stockholm 171 77 Sweden"], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/29c123916fbf4948a911560c1a259496.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/29c123916fbf4948a911560c1a259496"}}, "publications": [{"entity": "publication", "iuid": "29a0dbb0cdc140658fd8f1844edd0aad", "links": {"self": {"href": "https://publications.scilifelab.se/publication/29a0dbb0cdc140658fd8f1844edd0aad.json"}, "display": {"href": "https://publications.scilifelab.se/publication/29a0dbb0cdc140658fd8f1844edd0aad"}}, "title": "Chemogenomic Screening in a Patient\u2010Derived 3D Fatty Liver Disease Model Reveals the CHRM1\u2010TRPM8 Axis as a Novel Module for Targeted Intervention", "authors": [{"family": "Youhanna", "given": "Sonia", "initials": "S"}, {"family": "Kemas", "given": "Aurino M", "initials": "AM"}, {"family": "Wright", "given": "Shane C", "initials": "SC"}, {"family": "Zhong", "given": "Yi", "initials": "Y"}, {"family": "Klumpp", "given": "Britta", "initials": "B"}, {"family": "Klein", "given": "Kathrin", "initials": "K"}, {"family": "Motso", "given": "Aikaterini", "initials": "A"}, {"family": "Michel", "given": "Maurice", "initials": "M"}, {"family": "Ziegler", "given": "Nicole", "initials": "N"}, {"family": "Shang", "given": "Mingmei", "initials": "M"}, {"family": "Sabatier", "given": "Pierre", "initials": "P"}, {"family": "Kannt", "given": "Aimo", "initials": "A"}, {"family": "Sheng", "given": "Hongda", "initials": "H"}, {"family": "Oliva\u2010Vilarnau", "given": "Nuria", "initials": "N"}, {"family": "B\u00fcttner", "given": "Florian A", "initials": "FA"}, {"family": "Seashore\u2010Ludlow", "given": "Brinton", "initials": "B"}, {"family": "Schreiner", "given": "Jonas", "initials": "J"}, {"family": "Windbergs", "given": "Maike", "initials": "M"}, {"family": "Cornillet", "given": "Martin", "initials": "M"}, {"family": "Bj\u00f6rkstr\u00f6m", "given": "Niklas K", "initials": "NK"}, {"family": "H\u00fclsmeier", "given": "Andreas J", "initials": "AJ"}, {"family": "Hornemann", "given": "Thorsten", "initials": "T"}, {"family": "Olsen", "given": "Jesper V", "initials": "JV"}, {"family": "Wang", "given": "Yi", "initials": "Y"}, {"family": "Gramignoli", "given": "Roberto", "initials": "R"}, {"family": "Sundstr\u00f6m", "given": "Michael", "initials": "M"}, {"family": "Lauschke", "given": "Volker M", "initials": "VM", "orcid": "0000-0002-1140-6204", "researcher": {"href": "https://publications.scilifelab.se/researcher/29c123916fbf4948a911560c1a259496.json"}}], "type": "journal-article", "published": "2025-01-00", "journal": {"title": "Adv Sci (Weinh)", "issn": "2198-3844", "volume": "12", "issue": "3", "issn-l": null}, "abstract": null, "doi": "10.1002/advs.202407572", "pmid": null, "labels": {"Chemical Biology Consortium Sweden": "Service"}, "xrefs": [], "notes": [], "created": "2025-04-23T09:39:57.949Z", "modified": "2025-10-17T13:04:26.969Z"}, {"entity": "publication", "iuid": "72f3aa35636c456e84ca5ceb90728c2a", "links": {"self": {"href": "https://publications.scilifelab.se/publication/72f3aa35636c456e84ca5ceb90728c2a.json"}, "display": {"href": "https://publications.scilifelab.se/publication/72f3aa35636c456e84ca5ceb90728c2a"}}, "title": "Estrogen receptor activation remodels TEAD1 gene expression to alleviate hepatic steatosis.", "authors": [{"family": "Sommerauer", "given": "Christian", "initials": "C", "orcid": "0000-0001-7132-7172", "researcher": {"href": "https://publications.scilifelab.se/researcher/01320952391e4afe9b924dcf85b5d50a.json"}}, {"family": "Gallardo-Dodd", "given": "Carlos J", "initials": "CJ"}, {"family": "Savva", "given": "Christina", "initials": "C"}, {"family": "Hases", "given": "Linnea", "initials": "L"}, {"family": "Birgersson", "given": "Madeleine", "initials": "M"}, {"family": "Indukuri", "given": "Rajitha", "initials": "R"}, {"family": "Shen", "given": "Joanne X", "initials": "JX", "orcid": "0009-0008-0322-5615", "researcher": {"href": "https://publications.scilifelab.se/researcher/519a2d0afb6747c6ad3cf2b12c4d0bf3.json"}}, {"family": "Carravilla", "given": "Pablo", "initials": "P"}, {"family": "Geng", "given": "Keyi", "initials": "K"}, {"family": "N\u00f8rskov S\u00f8ndergaard", "given": "Jonas", "initials": "J", "orcid": "0000-0002-4438-6756", "researcher": {"href": "https://publications.scilifelab.se/researcher/d0125f039a4949d8a22b9048d7f6b7d8.json"}}, {"family": "Ferrer-Aumatell", "given": "Cl\u00e0udia", "initials": "C"}, {"family": "Mercier", "given": "Gr\u00e9goire", "initials": "G"}, {"family": "Sezgin", "given": "Erdinc", "initials": "E", "orcid": "0000-0002-4915-388X", "researcher": {"href": "https://publications.scilifelab.se/researcher/34d3b05d68d64f698ff08dc655d2fe26.json"}}, {"family": "Korach-Andr\u00e9", "given": "Marion", "initials": "M"}, {"family": "Petersson", "given": "Carl", "initials": "C"}, {"family": "Hagstr\u00f6m", "given": "Hannes", "initials": "H"}, {"family": "Lauschke", "given": "Volker M", "initials": "VM", "orcid": "0000-0002-1140-6204", "researcher": {"href": "https://publications.scilifelab.se/researcher/29c123916fbf4948a911560c1a259496.json"}}, {"family": "Archer", "given": "Amena", "initials": "A", "orcid": "0000-0002-0400-4151", "researcher": {"href": "https://publications.scilifelab.se/researcher/4502538fe3e84cb6a6618c972fa10b08.json"}}, {"family": "Williams", "given": "Cecilia", "initials": "C"}, {"family": "Kutter", "given": "Claudia", "initials": "C", "orcid": "0000-0002-8047-0058", "researcher": {"href": "https://publications.scilifelab.se/researcher/61f2e0e6c9be43ac946b318d080d5cca.json"}}], "type": "journal article", "published": "2024-04-00", "journal": {"title": "Mol. Syst. Biol.", "issn": "1744-4292", "volume": "20", "issue": "4", "pages": "374-402", "issn-l": "1744-4292"}, "abstract": "Sex-based differences in obesity-related hepatic malignancies suggest the protective roles of estrogen. Using a preclinical model, we dissected estrogen receptor (ER) isoform-driven molecular responses in high-fat diet (HFD)-induced liver diseases of male and female mice treated with or without an estrogen agonist by integrating liver multi-omics data. We found that selective ER activation recovers HFD-induced molecular and physiological liver phenotypes. HFD and systemic ER activation altered core liver pathways, beyond lipid metabolism, that are consistent between mice and primates. By including patient cohort data, we uncovered that ER-regulated enhancers govern central regulatory and metabolic genes with clinical significance in metabolic dysfunction-associated steatotic liver disease (MASLD) patients, including the transcription factor TEAD1. TEAD1 expression increased in MASLD patients, and its downregulation by short interfering RNA reduced intracellular lipid content. Subsequent TEAD small molecule inhibition improved steatosis in primary human hepatocyte spheroids by suppressing lipogenic pathways. Thus, TEAD1 emerged as a new therapeutic candidate whose inhibition ameliorates hepatic steatosis.", "doi": "10.1038/s44320-024-00024-x", "pmid": "38459198", "labels": {"NGI Short read": "Service", "NGI Stockholm (Genomics Production)": "Service", "National Genomics Infrastructure": "Service", "Integrated Microscopy Technologies Stockholm": "Service", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC10987545"}, {"db": "pii", "key": "10.1038/s44320-024-00024-x"}], "notes": [], "created": "2024-03-14T11:08:10.304Z", "modified": "2025-02-28T14:16:38.102Z"}, {"entity": "publication", "iuid": "24debc4b98554ac381461a8be3ba5b25", "links": {"self": {"href": "https://publications.scilifelab.se/publication/24debc4b98554ac381461a8be3ba5b25.json"}, "display": {"href": "https://publications.scilifelab.se/publication/24debc4b98554ac381461a8be3ba5b25"}}, "title": "Estrogen receptor activation remodelsTEAD1gene expression to alleviate nonalcoholic fatty liver disease", "authors": [{"family": "Sommerauer", "given": "Christian", "initials": "C", "orcid": "0000-0001-7132-7172", "researcher": {"href": "https://publications.scilifelab.se/researcher/01320952391e4afe9b924dcf85b5d50a.json"}}, {"family": "Gallardo-Dodd", "given": "Carlos J", "initials": "CJ", "orcid": "0000-0002-6086-0550", "researcher": {"href": "https://publications.scilifelab.se/researcher/0d80ebf009104cae8aaf56f9ad998c3b.json"}}, {"family": "Savva", "given": "Christina", "initials": "C", "orcid": "0000-0002-8019-1104", "researcher": {"href": "https://publications.scilifelab.se/researcher/9a4ce68039184f35922fe3dd337f0ced.json"}}, {"family": "Hases", "given": "Linnea", "initials": "L", "orcid": "0000-0001-6741-7204", "researcher": {"href": "https://publications.scilifelab.se/researcher/eeb5e09b88e94ab59a6ca9f8c457c5f4.json"}}, {"family": "Birgersson", "given": "Madeleine", "initials": "M"}, {"family": "Indukuri", "given": "Rajitha", "initials": "R", "orcid": "0000-0001-6570-842X", "researcher": {"href": "https://publications.scilifelab.se/researcher/94148ec0ffb74f9bb0243c18a1256c22.json"}}, {"family": "Shen", "given": "Joanne X", "initials": "JX", "orcid": "0009-0008-0322-5615", "researcher": {"href": "https://publications.scilifelab.se/researcher/519a2d0afb6747c6ad3cf2b12c4d0bf3.json"}}, {"family": "Carravilla", "given": "Pablo", "initials": "P", "orcid": "0000-0001-6592-7630", "researcher": {"href": "https://publications.scilifelab.se/researcher/b791a8068a724c179b731f0446b1737a.json"}}, {"family": "Geng", "given": "Keyi", "initials": "K", "orcid": "0000-0003-0892-7460", "researcher": {"href": "https://publications.scilifelab.se/researcher/e0ce637276ce4cb0b81f9f039d261c61.json"}}, {"family": "N\u00f8rskov S\u00f8ndergaard", "given": "Jonas", "initials": "J", "orcid": "0000-0002-4438-6756", "researcher": {"href": "https://publications.scilifelab.se/researcher/d0125f039a4949d8a22b9048d7f6b7d8.json"}}, {"family": "Ferrer-Aumatell", "given": "Cl\u00e0udia", "initials": "C", "orcid": "0009-0008-9828-6209", "researcher": {"href": "https://publications.scilifelab.se/researcher/a492fadc2d4e41028a5d103a87e214aa.json"}}, {"family": "Mercier", "given": "Gr\u00e9goire", "initials": "G", "orcid": "0009-0002-9966-9910", "researcher": {"href": "https://publications.scilifelab.se/researcher/b363ed8d40cd4bfdae8783c85922036b.json"}}, {"family": "Sezgin", "given": "Erdinc", "initials": "E", "orcid": "0000-0002-4915-388X", "researcher": {"href": "https://publications.scilifelab.se/researcher/34d3b05d68d64f698ff08dc655d2fe26.json"}}, {"family": "Korach-Andr\u00e9", "given": "Marion", "initials": "M", "orcid": "0000-0003-3292-9124", "researcher": {"href": "https://publications.scilifelab.se/researcher/407729e3f36742a890a54a8bac2e9662.json"}}, {"family": "Petersson", "given": "Carl", "initials": "C"}, {"family": "Hagstr\u00f6m", "given": "Hannes", "initials": "H", "orcid": "0000-0002-8474-1759", "researcher": {"href": "https://publications.scilifelab.se/researcher/5e5d3927690e4563a049c1703ecc4034.json"}}, {"family": "Lauschke", "given": "Volker M", "initials": "VM", "orcid": "0000-0002-1140-6204", "researcher": {"href": "https://publications.scilifelab.se/researcher/29c123916fbf4948a911560c1a259496.json"}}, {"family": "Archer", "given": "Amena", "initials": "A", "orcid": "0000-0002-0400-4151", "researcher": {"href": "https://publications.scilifelab.se/researcher/4502538fe3e84cb6a6618c972fa10b08.json"}}, {"family": "Williams", "given": "Cecilia", "initials": "C", "orcid": "0000-0002-0602-2062", "researcher": {"href": "https://publications.scilifelab.se/researcher/89989da8d4e64dd3a30b87fc62ceefae.json"}}, {"family": "Kutter", "given": "Claudia", "initials": "C", "orcid": "0000-0002-8047-0058", "researcher": {"href": "https://publications.scilifelab.se/researcher/61f2e0e6c9be43ac946b318d080d5cca.json"}}], "type": "posted-content", "published": "2023-09-08", "journal": {"title": "biorxiv", "issn": null, "issn-l": null, "volume": null, "issue": null, "pages": null}, "abstract": null, "doi": "10.1101/2023.09.07.556687", "pmid": null, "labels": {"Integrated Microscopy Technologies Stockholm": "Service"}, "xrefs": [], "notes": [], "created": "2024-01-10T11:47:13.965Z", "modified": "2025-12-18T19:46:10.899Z"}, {"entity": "publication", "iuid": "ac80952db59f49f3b1c15eec06392e65", "links": {"self": {"href": "https://publications.scilifelab.se/publication/ac80952db59f49f3b1c15eec06392e65.json"}, "display": {"href": "https://publications.scilifelab.se/publication/ac80952db59f49f3b1c15eec06392e65"}}, "title": "Two different isoforms of osteopontin modulate myelination and axonal integrity.", "authors": [{"family": "Nilsson", "given": "Gisela", "initials": "G", "orcid": "0000-0003-4423-5082", "researcher": {"href": "https://publications.scilifelab.se/researcher/c07681f5131b4c58bd71119c56f5d435.json"}}, {"family": "Mottahedin", "given": "Amin", "initials": "A", "orcid": "0000-0002-3677-2198", "researcher": {"href": "https://publications.scilifelab.se/researcher/82a4c6f53ae241edaf5147addb082567.json"}}, {"family": "Zelco", "given": "Aura", "initials": "A", "orcid": "0000-0002-5851-6355", "researcher": {"href": "https://publications.scilifelab.se/researcher/f66e84b556ab4454b96ba929cfa2b104.json"}}, {"family": "Lauschke", "given": "Volker M", "initials": "VM", "orcid": "0000-0002-1140-6204", "researcher": {"href": "https://publications.scilifelab.se/researcher/29c123916fbf4948a911560c1a259496.json"}}, {"family": "Ek", "given": "C Joakim", "initials": "CJ", "orcid": "0000-0002-5764-7679", "researcher": {"href": "https://publications.scilifelab.se/researcher/991c705e88dd472cae9e98ad540660ab.json"}}, {"family": "Song", "given": "Juan", "initials": "J"}, {"family": "Ardalan", "given": "Maryam", "initials": "M", "orcid": "0000-0003-3414-1584", "researcher": {"href": "https://publications.scilifelab.se/researcher/ceef7775ba414a1ab0f5039db2f0014b.json"}}, {"family": "Hua", "given": "Sha", "initials": "S"}, {"family": "Zhang", "given": "Xiaoli", "initials": "X", "orcid": "0000-0001-5111-9405", "researcher": {"href": "https://publications.scilifelab.se/researcher/3d5f1af1b5f3452d8db56bbe3eea440c.json"}}, {"family": "Mallard", "given": "Carina", "initials": "C", "orcid": "0000-0001-8953-919X", "researcher": {"href": "https://publications.scilifelab.se/researcher/2d0fa7de10554b5bb5a0e1ee64902aa1.json"}}, {"family": "Hagberg", "given": "Henrik", "initials": "H", "orcid": "0000-0003-3962-1448", "researcher": {"href": "https://publications.scilifelab.se/researcher/ca1150b5f07c441096f2716c0564e1d0.json"}}, {"family": "Leavenworth", "given": "Jianmei W", "initials": "JW", "orcid": "0000-0002-4100-9883", "researcher": {"href": "https://publications.scilifelab.se/researcher/d1b287368adf4edabe671e33ab56c550.json"}}, {"family": "Wang", "given": "Xiaoyang", "initials": "X", "orcid": "0000-0001-9717-8160", "researcher": {"href": "https://publications.scilifelab.se/researcher/70fbed19bca84bec93f558168444227a.json"}}], "type": "journal article", "published": "2023-08-00", "journal": {"title": "FASEB Bioadv", "issn": "2573-9832", "issn-l": null, "volume": "5", "issue": "8", "pages": "336-353"}, "abstract": "Abnormal myelination underlies the pathology of white matter diseases such as preterm white matter injury and multiple sclerosis. Osteopontin (OPN) has been suggested to play a role in myelination. Murine OPN mRNA is translated into a secreted isoform (sOPN) or an intracellular isoform (iOPN). Whether there is an isoform-specific involvement of OPN in myelination is unknown. Here we generated mouse models that either lacked both OPN isoforms in all cells (OPN-KO) or lacked sOPN systemically but expressed iOPN specifically in oligodendrocytes (OLs-iOPN-KI). Transcriptome analysis of isolated oligodendrocytes from the neonatal brain showed that genes and pathways related to increase of myelination and altered cell cycle control were enriched in the absence of the two OPN isoforms in OPN-KO mice compared to control mice. Accordingly, adult OPN-KO mice showed an increased axonal myelination, as revealed by transmission electron microscopy imaging, and increased expression of myelin-related proteins. In contrast, neonatal oligodendrocytes from OLs-iOPN-KI mice compared to control mice showed differential regulation of genes and pathways related to the increase of cell adhesion, motility, and vasculature development, and the decrease of axonal/neuronal development. OLs-iOPN-KI mice showed abnormal myelin formation in the early phase of myelination in young mice and signs of axonal degeneration in adulthood. These results suggest an OPN isoform-specific involvement, and a possible interplay between the isoforms, in myelination, and axonal integrity. Thus, the two isoforms of OPN need to be separately considered in therapeutic strategies targeting OPN in white matter injury and diseases.", "doi": "10.1096/fba.2023-00030", "pmid": "37554545", "labels": {"Clinical Genomics Gothenburg": "Service", "Integrated Microscopy Technologies Gothenburg": "Service", "Clinical Genomics": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC10405251"}, {"db": "pii", "key": "FBA21405"}], "notes": [], "created": "2023-11-30T22:35:19.238Z", "modified": "2023-12-03T13:28:50.770Z"}, {"entity": "publication", "iuid": "cf1d945512544bdabec3dbb006c0755d", "links": {"self": {"href": "https://publications.scilifelab.se/publication/cf1d945512544bdabec3dbb006c0755d.json"}, "display": {"href": "https://publications.scilifelab.se/publication/cf1d945512544bdabec3dbb006c0755d"}}, "title": "Human resident liver myeloid cells protect against metabolic stress in obesity.", "authors": [{"family": "Barreby", "given": "Emelie", "initials": "E", "orcid": "0000-0002-6467-5280", "researcher": {"href": "https://publications.scilifelab.se/researcher/994aee7303624575a8f634359e50fa31.json"}}, {"family": "Strunz", "given": "Benedikt", "initials": "B"}, {"family": "Nock", "given": "Sebastian", "initials": "S", "orcid": "0000-0001-5224-0047", "researcher": {"href": "https://publications.scilifelab.se/researcher/4121a5d5457b48e68e634cf638ee0418.json"}}, {"family": "Naudet", "given": "L\u00e9a", "initials": "L"}, {"family": "Shen", "given": "Joanne X", "initials": "JX"}, {"family": "Johansson", "given": "Helene", "initials": "H"}, {"family": "S\u00f6nnerborg", "given": "Isabella", "initials": "I"}, {"family": "Ma", "given": "Junjie", "initials": "J"}, {"family": "Urgard", "given": "Egon", "initials": "E"}, {"family": "Pallett", "given": "Laura J", "initials": "LJ", "orcid": "0000-0002-4161-9462", "researcher": {"href": "https://publications.scilifelab.se/researcher/60d13c747ab8459ab192b2c02dd12014.json"}}, {"family": "Hu", "given": "Yizhou", "initials": "Y", "orcid": "0000-0002-2635-0258", "researcher": {"href": "https://publications.scilifelab.se/researcher/2b75010a59c243279eaf9ca7af75315b.json"}}, {"family": "Fardellas", "given": "Achilleas", "initials": "A", "orcid": "0000-0003-2797-6962", "researcher": {"href": "https://publications.scilifelab.se/researcher/c562a71c56674a978f60306446fc7cd1.json"}}, {"family": "Azzimato", "given": "Valerio", "initials": "V", "orcid": "0000-0003-4141-961X", "researcher": {"href": "https://publications.scilifelab.se/researcher/469cbad28708470f9998233f0c5fafc6.json"}}, {"family": "Vankova", "given": "Ana", "initials": "A", "orcid": "0000-0003-0955-8097", "researcher": {"href": "https://publications.scilifelab.se/researcher/9a846c1e204b4369aa677cf2867d37b3.json"}}, {"family": "Levi", "given": "Laura", "initials": "L"}, {"family": "Morgantini", "given": "Cecilia", "initials": "C", "orcid": "0000-0003-3142-2508", "researcher": {"href": "https://publications.scilifelab.se/researcher/297e0d58ae7a45a29d5dee1f7e057809.json"}}, {"family": "Maini", "given": "Mala K", "initials": "MK", "orcid": "0000-0001-6384-1462", "researcher": {"href": "https://publications.scilifelab.se/researcher/1fa684c9aaad4849a48510eb85ff93be.json"}}, {"family": "St\u00e5l", "given": "Per", "initials": "P"}, {"family": "Rosshart", "given": "Stephan P", "initials": "SP", "orcid": "0000-0002-6989-8686", "researcher": {"href": "https://publications.scilifelab.se/researcher/daac4d2d5dd54205bf4d07b1066fad97.json"}}, {"family": "Coquet", "given": "Jonathan M", "initials": "JM", "orcid": "0000-0002-5967-4857", "researcher": {"href": "https://publications.scilifelab.se/researcher/1b74959951024d6ea9712b55f115652e.json"}}, {"family": "Nowak", "given": "Greg", "initials": "G", "orcid": "0000-0001-8766-8860", "researcher": {"href": "https://publications.scilifelab.se/researcher/857e73fc97484151ac37526d45c532f6.json"}}, {"family": "N\u00e4slund", "given": "Erik", "initials": "E", "orcid": "0000-0002-0166-6344", "researcher": {"href": "https://publications.scilifelab.se/researcher/0d2603ac3ac34d75b7167ad1b93d344b.json"}}, {"family": "Lauschke", "given": "Volker M", "initials": "VM", "orcid": "0000-0002-1140-6204", "researcher": {"href": "https://publications.scilifelab.se/researcher/29c123916fbf4948a911560c1a259496.json"}}, {"family": "Ellis", "given": "Ewa", "initials": "E", "orcid": "0000-0002-3057-5337", "researcher": {"href": "https://publications.scilifelab.se/researcher/82ef7cf7903140cfbb7ff8d6f4961dde.json"}}, {"family": "Bj\u00f6rkstr\u00f6m", "given": "Niklas K", "initials": "NK", "orcid": "0000-0002-0967-076X", "researcher": {"href": "https://publications.scilifelab.se/researcher/bf38521350ae490099f01bda1d10be4c.json"}}, {"family": "Chen", "given": "Ping", "initials": "P", "orcid": "0000-0002-8068-1891", "researcher": {"href": "https://publications.scilifelab.se/researcher/5b2940a8aca44f28b88de7e3598acd53.json"}}, {"family": "Aouadi", "given": "Myriam", "initials": "M", "orcid": "0000-0001-6256-7107", "researcher": {"href": "https://publications.scilifelab.se/researcher/7ea7bb2be61a4e818685466d1005659d.json"}}], "type": "journal article", "published": "2023-07-00", "journal": {"title": "Nat Metab", "issn": "2522-5812", "volume": "5", "issue": "7", "pages": "1188-1203", "issn-l": "2522-5812"}, "abstract": "Although multiple populations of macrophages have been described in the human liver, their function and turnover in patients with obesity at high risk of developing non-alcoholic fatty liver disease (NAFLD) and cirrhosis are currently unknown. Herein, we identify a specific human population of resident liver myeloid cells that protects against the metabolic impairment associated with obesity. By studying the turnover of liver myeloid cells in individuals undergoing liver transplantation, we find that liver myeloid cell turnover differs between humans and mice. Using single-cell techniques and flow cytometry, we determine that the proportion of the protective resident liver myeloid cells, denoted liver myeloid cells 2 (LM2), decreases during obesity. Functional validation approaches using human 2D and 3D cultures reveal that the presence of LM2 ameliorates the oxidative stress associated with obese conditions. Our study indicates that resident myeloid cells could be a therapeutic target to decrease the oxidative stress associated with NAFLD.", "doi": "10.1038/s42255-023-00834-7", "pmid": "37414931", "labels": {"Spatial Proteomics": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC10365994"}, {"db": "pii", "key": "10.1038/s42255-023-00834-7"}], "notes": [], "created": "2023-11-21T15:12:13.445Z", "modified": "2023-11-21T15:12:13.896Z"}, {"entity": "publication", "iuid": "1d0288987240422cb265dd17e35fc36a", "links": {"self": {"href": "https://publications.scilifelab.se/publication/1d0288987240422cb265dd17e35fc36a.json"}, "display": {"href": "https://publications.scilifelab.se/publication/1d0288987240422cb265dd17e35fc36a"}}, "title": "Enzymatically dissociated muscle fibers display rapid dedifferentiation and impaired mitochondrial calcium control", "authors": [{"family": "Gineste", "given": "Charlotte", "initials": "C", "orcid": "0000-0001-8515-089X", "researcher": {"href": "https://publications.scilifelab.se/researcher/f4cf78e803e64549b275b1af3a15a634.json"}}, {"family": "Youhanna", "given": "Sonia", "initials": "S", "orcid": "0000-0002-9376-8478", "researcher": {"href": "https://publications.scilifelab.se/researcher/edea26d5bc0047bb8f1b6de516539a6c.json"}}, {"family": "Vorrink", "given": "Sabine U", "initials": "SU", "orcid": "0000-0002-2807-1316", "researcher": {"href": "https://publications.scilifelab.se/researcher/e83df3c8339749739f0e6ddee80aed19.json"}}, {"family": "Henriksson", "given": "Sara", "initials": "S", "orcid": "0000-0003-1615-0583", "researcher": {"href": "https://publications.scilifelab.se/researcher/934e6f18e3c94684be715e7bdc28d9b7.json"}}, {"family": "Hern\u00e1ndez", "given": "Andr\u00e9s", "initials": "A"}, {"family": "Cheng", "given": "Arthur J", "initials": "AJ", "orcid": "0000-0003-3862-2967", "researcher": {"href": "https://publications.scilifelab.se/researcher/8752d4f39f5745a186f52f7ad2e1048e.json"}}, {"family": "Chaillou", "given": "Thomas", "initials": "T", "orcid": "0000-0002-5322-4150", "researcher": {"href": "https://publications.scilifelab.se/researcher/6456c7f8f9e4418e81c0b49e9ff5e487.json"}}, {"family": "Buttgereit", "given": "Andreas", "initials": "A"}, {"family": "Schneidereit", "given": "Dominik", "initials": "D", "orcid": "0000-0001-7351-6428", "researcher": {"href": "https://publications.scilifelab.se/researcher/3d7b35ecc2d6409ab85f7e0b26a86544.json"}}, {"family": "Friedrich", "given": "Oliver", "initials": "O", "orcid": "0000-0003-2238-2049", "researcher": {"href": "https://publications.scilifelab.se/researcher/feb5ea0415894026948aca80f716f580.json"}}, {"family": "Hultenby", "given": "Kjell", "initials": "K"}, {"family": "Bruton", "given": "Joseph D", "initials": "JD", "orcid": "0000-0002-6985-099X", "researcher": {"href": "https://publications.scilifelab.se/researcher/2b024fc7001c4200818e205acb4b50b9.json"}}, {"family": "Ivarsson", "given": "Niklas", "initials": "N"}, {"family": "Sandblad", "given": "Linda", "initials": "L", "orcid": "0000-0003-3492-3287", "researcher": {"href": "https://publications.scilifelab.se/researcher/070825e0190a4e9a932e79663d2bc89f.json"}}, {"family": "Lauschke", "given": "Volker M", "initials": "VM", "orcid": "0000-0002-1140-6204", "researcher": {"href": "https://publications.scilifelab.se/researcher/29c123916fbf4948a911560c1a259496.json"}}, {"family": "Westerblad", "given": "H\u00e5kan", "initials": "H", "orcid": "0000-0002-8180-3029", "researcher": {"href": "https://publications.scilifelab.se/researcher/eef65bed3cce440481d1932997c8fef0.json"}}], "type": "journal-article", "published": "2022-11-00", "journal": {"title": "iScience", "issn": "2589-0042", "issn-l": "2589-0042", "volume": null, "issue": null, "pages": "105654"}, "abstract": null, "doi": "10.1016/j.isci.2022.105654", "pmid": "36479146", "labels": {"Integrated Microscopy Technologies Ume\u00e5": "Collaborative"}, "xrefs": [], "notes": [], "created": "2022-11-29T10:52:54.580Z", "modified": "2023-06-19T13:29:27.647Z"}, {"entity": "publication", "iuid": "5446fda678b44232a03fb58e253bbb2f", "links": {"self": {"href": "https://publications.scilifelab.se/publication/5446fda678b44232a03fb58e253bbb2f.json"}, "display": {"href": "https://publications.scilifelab.se/publication/5446fda678b44232a03fb58e253bbb2f"}}, "title": "A 3D Cell Culture Model Identifies Wnt/\u03b2-Catenin Mediated Inhibition of p53 as a Critical Step during Human Hepatocyte Regeneration.", "authors": [{"family": "Oliva-Vilarnau", "given": "Nuria", "initials": "N", "orcid": "0000-0002-3083-6701", "researcher": {"href": "https://publications.scilifelab.se/researcher/29e93281648540e9ae407c2cefbc542e.json"}}, {"family": "Vorrink", "given": "Sabine U", "initials": "SU"}, {"family": "Ingelman-Sundberg", "given": "Magnus", "initials": "M"}, {"family": "Lauschke", "given": "Volker M", "initials": "VM", "orcid": "0000-0002-1140-6204", "researcher": {"href": "https://publications.scilifelab.se/researcher/29c123916fbf4948a911560c1a259496.json"}}], "type": "journal article", "published": "2020-08-00", "journal": {"title": "Adv Sci (Weinh)", "issn": "2198-3844", "volume": "7", "issue": "15", "pages": "2000248", "issn-l": null}, "abstract": "The liver is a highly regenerative organ. While mature hepatocytes under homeostatic conditions are largely quiescent, upon injury, they rapidly enter the cell cycle to recover the damaged tissue. In rodents, a variety of injury models have provided important insights into the molecular underpinnings that govern the proliferative activation of quiescent hepatocytes. However, little is known about the molecular mechanisms of human hepatocyte regeneration and experimental methods to expand primary human hepatocytes (PHH). Here, a 3D spheroid model of PHH is established to study hepatocyte regeneration and integrative time-lapse multi-omics analyses show that upon isolation from the native liver PHH acquire a regenerative phenotype, as seen in vivo upon partial hepatectomy. However, proliferation is limited. By analyzing global promoter motif activities, it is predicted that activation of Wnt/\u03b2-catenin and inhibition of p53 signaling are critical factors required for human hepatocyte proliferation. Functional validations reveal that activation of Wnt signaling through external cues alone is sufficient to inhibit p53 and its proliferative senescence-inducing target PAI1 (SERPINE1) and drive proliferation of >50% of all PHH. A scalable 3D culture model is established to study the molecular and cellular biology of human hepatocyte regeneration. By using this model, an essential role of Wnt/\u03b2-catenin signaling during human hepatocyte regeneration is identified.", "doi": "10.1002/advs.202000248", "pmid": "32775153", "labels": {"NGI Stockholm (Genomics Production)": "Service", "National Genomics Infrastructure": "Service", "NGI Stockholm (Genomics Applications)": "Service", "Global Proteomics and Proteogenomics": "Service"}, "xrefs": [{"db": "pii", "key": "ADVS1906"}, {"db": "pmc", "key": "PMC7404138"}], "notes": [], "created": "2020-12-07T16:34:35.595Z", "modified": "2022-03-29T13:44:10.380Z"}]}