{"entity": "researcher", "timestamp": "2026-08-13T19:19:21.522Z", "family": "Andr\u00e9asson", "given": "Joakim", "initials": "J", "orcid": "0000-0003-4695-7943", "affiliations": ["Department of Chemistry and Chemical Engineering, Physical Chemistry, Chalmers University of Technology, 41296, G\u00f6teborg, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/28eb5affb5664c54be9171dfce6bea15.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/28eb5affb5664c54be9171dfce6bea15"}}, "publications": [{"entity": "publication", "iuid": "bb84c8f0da2546d197d9be264a758437", "links": {"self": {"href": "https://publications.scilifelab.se/publication/bb84c8f0da2546d197d9be264a758437.json"}, "display": {"href": "https://publications.scilifelab.se/publication/bb84c8f0da2546d197d9be264a758437"}}, "title": "Design and application of a fluorescent probe for imaging of endogenous Bruton's tyrosine kinase with preserved enzymatic activity.", "authors": [{"family": "Valaka", "given": "Anna P", "initials": "AP", "orcid": "0009-0008-7007-6971", "researcher": {"href": "https://publications.scilifelab.se/researcher/90c945c6f7b84acc8fc0097ec194332e.json"}}, {"family": "Nystr\u00f6m", "given": "Hampus", "initials": "H", "orcid": "0009-0006-8139-3839", "researcher": {"href": "https://publications.scilifelab.se/researcher/a763c1525ea446bd913291a2599ea9f7.json"}}, {"family": "H\u00e5versen", "given": "Liliana", "initials": "L"}, {"family": "Benitez-Martin", "given": "Carlos", "initials": "C", "orcid": "0000-0003-1821-9388", "researcher": {"href": "https://publications.scilifelab.se/researcher/d1fcdd6e053b462998fe7f463be60d96.json"}}, {"family": "Sch\u00e4fer", "given": "Clara", "initials": "C", "orcid": "0000-0001-7634-3980", "researcher": {"href": "https://publications.scilifelab.se/researcher/58fcf0b78e574d5f9a52fcab14632117.json"}}, {"family": "Jang", "given": "Woo Suk", "initials": "WS"}, {"family": "Camponeschi", "given": "Alessandro", "initials": "A", "orcid": "0000-0002-6472-2438", "researcher": {"href": "https://publications.scilifelab.se/researcher/ccbaffafd6a24f4abed1e402219fa472.json"}}, {"family": "Andr\u00e9asson", "given": "Joakim", "initials": "J", "orcid": "0000-0003-4695-7943", "researcher": {"href": "https://publications.scilifelab.se/researcher/28eb5affb5664c54be9171dfce6bea15.json"}}, {"family": "Bor\u00e9n", "given": "Jan", "initials": "J", "orcid": "0000-0003-0786-8091", "researcher": {"href": "https://publications.scilifelab.se/researcher/1e85f6d287ce4c60a7b35b287efb4f79.json"}}, {"family": "Gr\u00f8tli", "given": "Morten", "initials": "M", "orcid": "0000-0003-3621-4222", "researcher": {"href": "https://publications.scilifelab.se/researcher/764706606bcb4afba1150af332c0f124.json"}}], "type": "journal article", "published": "2025-04-02", "journal": {"title": "RSC Chem Biol", "issn": "2633-0679", "issn-l": null, "volume": "6", "issue": "4", "pages": "618-629"}, "abstract": "Fluorophore integration into proteins within living cells is essential for exploring proteins in their natural environment. Bruton's tyrosine kinase (BTK), is a validated oncology target and is crucial for B cell proliferation and activation. Developing BTK-labelling probes is key to understand BTK's dynamic signalling pathway. In this work, we aimed to develop a novel fluorescent labelling probe for endogenous BTK imaging while preserving its enzymatic activity. Evobrutinib, a second-generation BTK inhibitor with high selectivity, was chosen as the scaffold. We designed two probes, Evo-1 and Evo-2, with a BODIPY fluorescent group, guided by molecular modelling. The synthesis was achieved using optimised Suzuki-Miyaura cross-coupling and amide coupling reactions. Biochemical assays confirmed covalent binding to Cys481 of BTK while preserving its enzymatic activity. Labelling of endogenous BTK with Evo-2 with reduced off-target effects in Ramos cells was validated in cellular assays. The dynamic signalling pathway of BTK in its native environment was investigated by confocal microscopy with Evo-2. This methodology is a valuable asset in the chemical biology toolbox for studying protein dynamics and interactions in real time without interfering with the protein activity.", "doi": "10.1039/d4cb00313f", "pmid": "40026844", "labels": {"Glycoproteomics and MS Proteomics": "Service", "Integrated Microscopy Technologies Gothenburg": "Service", "Swedish NMR Centre": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC11867108"}, {"db": "pii", "key": "d4cb00313f"}], "notes": [], "created": "2025-10-23T16:03:12.593Z", "modified": "2025-11-27T13:34:09.343Z"}, {"entity": "publication", "iuid": "e2e453cf6e8f4cdba5b424821324021d", "links": {"self": {"href": "https://publications.scilifelab.se/publication/e2e453cf6e8f4cdba5b424821324021d.json"}, "display": {"href": "https://publications.scilifelab.se/publication/e2e453cf6e8f4cdba5b424821324021d"}}, "title": "All-photonic kinase inhibitors: light-controlled release-and-report inhibition.", "authors": [{"family": "Fleming", "given": "Cassandra L", "initials": "CL", "orcid": "0000-0002-7730-7305", "researcher": {"href": "https://publications.scilifelab.se/researcher/a29a83ba2b3444768821489dc667279e.json"}}, {"family": "Benitez-Martin", "given": "Carlos", "initials": "C", "orcid": "0000-0003-1821-9388", "researcher": {"href": "https://publications.scilifelab.se/researcher/d1fcdd6e053b462998fe7f463be60d96.json"}}, {"family": "Bernson", "given": "Elin", "initials": "E", "orcid": "0000-0001-6414-5725", "researcher": {"href": "https://publications.scilifelab.se/researcher/ced3ddbde2ad4f28b28b622d817b2e59.json"}}, {"family": "Xu", "given": "Yongjin", "initials": "Y"}, {"family": "Kristenson", "given": "Linnea", "initials": "L"}, {"family": "Inghardt", "given": "Tord", "initials": "T", "orcid": "0000-0002-4804-9474", "researcher": {"href": "https://publications.scilifelab.se/researcher/bc8a41790a034fe48cce02c8e7034e34.json"}}, {"family": "Lundb\u00e4ck", "given": "Thomas", "initials": "T", "orcid": "0000-0002-8145-7808", "researcher": {"href": "https://publications.scilifelab.se/researcher/e13df787cb884549bcf333aba4e6f010.json"}}, {"family": "Thor\u00e9n", "given": "Fredrik B", "initials": "FB", "orcid": "0000-0003-2167-7451", "researcher": {"href": "https://publications.scilifelab.se/researcher/6e8a4846c6f44c0793f4cb5d73b66de6.json"}}, {"family": "Gr\u00f8tli", "given": "Morten", "initials": "M", "orcid": "0000-0003-3621-4222", "researcher": {"href": "https://publications.scilifelab.se/researcher/764706606bcb4afba1150af332c0f124.json"}}, {"family": "Andr\u00e9asson", "given": "Joakim", "initials": "J", "orcid": "0000-0003-4695-7943", "researcher": {"href": "https://publications.scilifelab.se/researcher/28eb5affb5664c54be9171dfce6bea15.json"}}], "type": "journal article", "published": "2024-05-08", "journal": {"title": "Chem. Sci.", "issn": "2041-6520", "volume": "15", "issue": "18", "pages": "6897-6905", "issn-l": null}, "abstract": "Light-responsive molecular tools targeting kinases affords one the opportunity to study the underlying cellular function of selected kinases. In efforts to externally control lymphocyte-specific protein tyrosine kinase (LCK) activity, the development of release-and-report LCK inhibitors is described, in which (i) the release of the active kinase inhibitor can be controlled externally with light; and (ii) fluorescence is employed to report both the release and binding of the active kinase inhibitor. This introduces an unprecedented all-photonic method for users to both control and monitor real-time inhibitory activity. A functional cellular assay demonstrated light-mediated LCK inhibition in natural killer cells. The use of coumarin-derived caging groups resulted in rapid cellular uptake and non-specific intracellular localisation, while a BODIPY-derived caging group predominately localised in the cellular membrane. This concept of release-and-report inhibitors has the potential to be extended to other biorelevant targets where both spatiotemporal control in a cellular setting and a reporting mechanism would be beneficial.", "doi": "10.1039/d4sc00390j", "pmid": "38725520", "labels": {"Integrated Microscopy Technologies Gothenburg": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC11077529"}, {"db": "pii", "key": "d4sc00390j"}], "notes": [], "created": "2024-11-15T12:04:25.342Z", "modified": "2024-11-15T12:04:25.556Z"}, {"entity": "publication", "iuid": "e3d59e32739d4f7e93ec76c6f809a20d", "links": {"self": {"href": "https://publications.scilifelab.se/publication/e3d59e32739d4f7e93ec76c6f809a20d.json"}, "display": {"href": "https://publications.scilifelab.se/publication/e3d59e32739d4f7e93ec76c6f809a20d"}}, "title": "Design and development of a photoswitchable DFG-out kinase inhibitor.", "authors": [{"family": "Xu", "given": "Yongjin", "initials": "Y"}, {"family": "Gao", "given": "Chunxia", "initials": "C"}, {"family": "H\u00e5versen", "given": "Liliana", "initials": "L"}, {"family": "Lundb\u00e4ck", "given": "Thomas", "initials": "T", "orcid": "0000-0002-8145-7808", "researcher": {"href": "https://publications.scilifelab.se/researcher/e13df787cb884549bcf333aba4e6f010.json"}}, {"family": "Andr\u00e9asson", "given": "Joakim", "initials": "J", "orcid": "0000-0003-4695-7943", "researcher": {"href": "https://publications.scilifelab.se/researcher/28eb5affb5664c54be9171dfce6bea15.json"}}, {"family": "Gr\u00f8tli", "given": "Morten", "initials": "M", "orcid": "0000-0003-3621-4222", "researcher": {"href": "https://publications.scilifelab.se/researcher/764706606bcb4afba1150af332c0f124.json"}}], "type": "journal article", "published": "2021-09-30", "journal": {"title": "Chem. Commun. (Camb.)", "issn": "1364-548X", "issn-l": "1359-7345", "volume": "57", "issue": "78", "pages": "10043-10046"}, "abstract": "We report the synthesis and characterisation of a photoswitchable DFG-out kinase inhibitor. Photocontrol of the target kinase in both enzymatic and living cell assays is demonstrated.", "doi": "10.1039/d1cc04125h", "pmid": "34505602", "labels": {"Chemical Biology Consortium Sweden": "Service"}, "xrefs": [], "notes": [], "created": "2021-11-03T11:52:31.970Z", "modified": "2025-10-17T13:04:28.037Z"}, {"entity": "publication", "iuid": "f5196895ac8a4cebb5143f36ce3b1737", "links": {"self": {"href": "https://publications.scilifelab.se/publication/f5196895ac8a4cebb5143f36ce3b1737.json"}, "display": {"href": "https://publications.scilifelab.se/publication/f5196895ac8a4cebb5143f36ce3b1737"}}, "title": "Toward Two-Photon Absorbing Dyes with Unusually Potentiated Nonlinear Fluorescence Response.", "authors": [{"family": "Benitez-Martin", "given": "Carlos", "initials": "C", "orcid": "0000-0003-1821-9388", "researcher": {"href": "https://publications.scilifelab.se/researcher/d1fcdd6e053b462998fe7f463be60d96.json"}}, {"family": "Li", "given": "Shiming", "initials": "S"}, {"family": "Dominguez-Alfaro", "given": "Antonio", "initials": "A"}, {"family": "Najera", "given": "Francisco", "initials": "F", "orcid": "0000-0002-1635-5514", "researcher": {"href": "https://publications.scilifelab.se/researcher/59a60d855d794d45a9fff4746867037f.json"}}, {"family": "P\u00e9rez-Inestrosa", "given": "Ezequiel", "initials": "E", "orcid": "0000-0001-7546-5273", "researcher": {"href": "https://publications.scilifelab.se/researcher/5ecc62575cbc43d9bfb86efd8299499c.json"}}, {"family": "Pischel", "given": "Uwe", "initials": "U", "orcid": "0000-0001-8893-9829", "researcher": {"href": "https://publications.scilifelab.se/researcher/f834b537191b46659d27846e05238ef3.json"}}, {"family": "Andr\u00e9asson", "given": "Joakim", "initials": "J", "orcid": "0000-0003-4695-7943", "researcher": {"href": "https://publications.scilifelab.se/researcher/28eb5affb5664c54be9171dfce6bea15.json"}}], "type": "journal article", "published": "2020-09-02", "journal": {"title": "J. Am. Chem. Soc.", "issn": "1520-5126", "volume": "142", "issue": "35", "pages": "14854-14858", "issn-l": "0002-7863"}, "abstract": "The combination of two two-photon-induced processes in a F\u00f6rster resonance energy transfer (FRET)-operated photochromic fluorene-dithienylethene dyad lays the foundation for the observation of a quartic dependence of the fluorescence signal on the excitation light intensity. While this photophysical behavior is predicted for a four-photon absorbing dye, the herein proposed approach opens the way to use two-photon absorbing dyes, reaching the same performance. Hence, the spatial resolution limit, being a critical parameter for applications in fluorescence imaging or data storage with common two-photon absorbing dyes, is dramatically improved.", "doi": "10.1021/jacs.0c07377", "pmid": "32799520", "labels": {"Integrated Microscopy Technologies Gothenburg": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC7498150"}], "notes": [], "created": "2023-02-16T08:04:03.866Z", "modified": "2023-02-16T08:04:04.030Z"}, {"entity": "publication", "iuid": "d5b820ef1ecb4e649af639d9aba5a970", "links": {"self": {"href": "https://publications.scilifelab.se/publication/d5b820ef1ecb4e649af639d9aba5a970.json"}, "display": {"href": "https://publications.scilifelab.se/publication/d5b820ef1ecb4e649af639d9aba5a970"}}, "title": "A Fluorescent Kinase Inhibitor that Exhibits Diagnostic Changes in Emission upon Binding.", "authors": [{"family": "Fleming", "given": "Cassandra L", "initials": "CL", "orcid": "0000-0002-7730-7305", "researcher": {"href": "https://publications.scilifelab.se/researcher/a29a83ba2b3444768821489dc667279e.json"}}, {"family": "Sandoz", "given": "Patrick A", "initials": "PA", "orcid": "0000-0002-8379-7267", "researcher": {"href": "https://publications.scilifelab.se/researcher/acd737132e4b4b378257ae8f0316b3a2.json"}}, {"family": "Inghardt", "given": "Tord", "initials": "T", "orcid": "0000-0002-4804-9474", "researcher": {"href": "https://publications.scilifelab.se/researcher/bc8a41790a034fe48cce02c8e7034e34.json"}}, {"family": "\u00d6nfelt", "given": "Bj\u00f6rn", "initials": "B", "orcid": "0000-0001-5178-7593", "researcher": {"href": "https://publications.scilifelab.se/researcher/14bcdcf94cab40ff92319869d243f5d8.json"}}, {"family": "Gr\u00f8tli", "given": "Morten", "initials": "M", "orcid": "0000-0003-3621-4222", "researcher": {"href": "https://publications.scilifelab.se/researcher/764706606bcb4afba1150af332c0f124.json"}}, {"family": "Andr\u00e9asson", "given": "Joakim", "initials": "J", "orcid": "0000-0003-4695-7943", "researcher": {"href": "https://publications.scilifelab.se/researcher/28eb5affb5664c54be9171dfce6bea15.json"}}], "type": "journal article", "published": "2019-10-14", "journal": {"title": "Angew. Chem. Int. Ed. Engl.", "issn": "1521-3773", "volume": "58", "issue": "42", "pages": "15000-15004", "issn-l": "1433-7851"}, "abstract": "The development of a fluorescent LCK inhibitor that exhibits favourable solvatochromic properties upon binding the kinase is described. Fluorescent properties were realised through the inclusion of a prodan-derived fluorophore into the pharmacophore of an ATP-competitive kinase inhibitor. Fluorescence titration experiments demonstrate the solvatochromic properties of the inhibitor, in which dramatic increase in emission intensity and hypsochromic shift in emission maxima are clearly observed upon binding LCK. Microscopy experiments in cellular contexts together with flow cytometry show that the fluorescence intensity of the inhibitor correlates with the LCK concentration. Furthermore, multiphoton microscopy experiments demonstrate both the rapid cellular uptake of the inhibitor and that the two-photon cross section of the inhibitor is amenable for excitation at 700 nm.", "doi": "10.1002/anie.201909536", "pmid": "31411364", "labels": {"Integrated Microscopy Technologies Gothenburg": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC6851755"}], "notes": [], "created": "2020-01-23T16:40:34.671Z", "modified": "2021-06-18T13:56:45.251Z"}]}