{"entity": "researcher", "timestamp": "2026-04-16T18:11:51.863Z", "family": "Fierz", "given": "Beat", "initials": "B", "orcid": "0000-0002-2991-3044", "affiliations": ["\u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL), SB ISIC LCBM, Station 6, CH-1015, Lausanne, Switzerland. beat.fierz@epfl.ch."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/28e2af5e821949aea4ef24d1572be652.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/28e2af5e821949aea4ef24d1572be652"}}, "publications": [{"entity": "publication", "iuid": "abba2963b1494fd99261f53318783d0c", "links": {"self": {"href": "https://publications.scilifelab.se/publication/abba2963b1494fd99261f53318783d0c.json"}, "display": {"href": "https://publications.scilifelab.se/publication/abba2963b1494fd99261f53318783d0c"}}, "title": "CENP-A and CENP-B collaborate to create an open centromeric chromatin state.", "authors": [{"family": "Nagpal", "given": "Harsh", "initials": "H"}, {"family": "Ali-Ahmad", "given": "Ahmad", "initials": "A", "orcid": "0000-0002-9021-5405", "researcher": {"href": "https://publications.scilifelab.se/researcher/de1dff855d3a464ebfaa688560e1070f.json"}}, {"family": "Hirano", "given": "Yasuhiro", "initials": "Y", "orcid": "0000-0001-7339-4053", "researcher": {"href": "https://publications.scilifelab.se/researcher/62b67aa9b7ac42779562ace608d27ba2.json"}}, {"family": "Cai", "given": "Wei", "initials": "W", "orcid": "0000-0001-7482-7033", "researcher": {"href": "https://publications.scilifelab.se/researcher/93eaa15b823a48abb3f876db3e72bc5b.json"}}, {"family": "Halic", "given": "Mario", "initials": "M", "orcid": "0000-0002-0061-7372", "researcher": {"href": "https://publications.scilifelab.se/researcher/12af3cfa4f55436ea4fc7d64c378229e.json"}}, {"family": "Fukagawa", "given": "Tatsuo", "initials": "T", "orcid": "0000-0001-8564-6852", "researcher": {"href": "https://publications.scilifelab.se/researcher/0a66fb662b5440fb9e1116ecf15403cf.json"}}, {"family": "Sekuli\u0107", "given": "Nikolina", "initials": "N", "orcid": "0000-0002-8027-9114", "researcher": {"href": "https://publications.scilifelab.se/researcher/711df7e0192f4434a448deec7e5c1c14.json"}}, {"family": "Fierz", "given": "Beat", "initials": "B", "orcid": "0000-0002-2991-3044", "researcher": {"href": "https://publications.scilifelab.se/researcher/28e2af5e821949aea4ef24d1572be652.json"}}], "type": "journal article", "published": "2023-12-12", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "14", "issue": "1", "pages": "8227", "issn-l": "2041-1723"}, "abstract": "Centromeres are epigenetically defined via the presence of the histone H3 variant CENP-A. Contacting CENP-A nucleosomes, the constitutive centromere associated network (CCAN) and the kinetochore assemble, connecting the centromere to spindle microtubules during cell division. The DNA-binding centromeric protein CENP-B is involved in maintaining centromere stability and, together with CENP-A, shapes the centromeric chromatin state. The nanoscale organization of centromeric chromatin is not well understood. Here, we use single-molecule fluorescence and cryoelectron microscopy (cryoEM) to show that CENP-A incorporation establishes a dynamic and open chromatin state. The increased dynamics of CENP-A chromatin create an opening for CENP-B DNA access. In turn, bound CENP-B further opens the chromatin fiber structure and induces nucleosomal DNA unwrapping. Finally, removal of CENP-A increases CENP-B mobility in cells. Together, our studies show that the two centromere-specific proteins collaborate to reshape chromatin structure, enabling the binding of centromeric factors and establishing a centromeric chromatin state.", "doi": "10.1038/s41467-023-43739-5", "pmid": "38086807", "labels": {"Cryo-EM": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC10716449"}, {"db": "pii", "key": "10.1038/s41467-023-43739-5"}], "notes": [], "created": "2023-12-22T11:43:10.070Z", "modified": "2024-11-27T14:50:35.560Z"}]}