{"entity": "researcher", "timestamp": "2026-08-11T08:21:29.993Z", "family": "Larsson", "given": "Anders", "initials": "A", "orcid": "0000-0003-3161-0402", "affiliations": [], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/2276de26382b402aa384ac231f30f156.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/2276de26382b402aa384ac231f30f156"}}, "publications": [{"entity": "publication", "iuid": "52e091fbbc604aaab3844a78dc652c29", "links": {"self": {"href": "https://publications.scilifelab.se/publication/52e091fbbc604aaab3844a78dc652c29.json"}, "display": {"href": "https://publications.scilifelab.se/publication/52e091fbbc604aaab3844a78dc652c29"}}, "title": "Mapping Interactions Between Cytokines, Chemokines, Growth Factors, and Conventional Biomarkers in COVID-19 ICU-Patients", "authors": [{"family": "Eriksson", "given": "Mats B", "initials": "MB", "orcid": "0000-0002-3178-4210", "researcher": {"href": "https://publications.scilifelab.se/researcher/cfcf6caaeeea422997c259d802e7e6f8.json"}}, {"family": "Marks-Hultstr\u00f6m", "given": "Michael", "initials": "M", "orcid": "0000-0003-4675-1099", "researcher": {"href": "https://publications.scilifelab.se/researcher/c9a74d3380a24c31930e6e671e685b5b.json"}}, {"family": "\u00c5berg", "given": "Mikael", "initials": "M", "orcid": "0000-0002-7858-8233", "researcher": {"href": "https://publications.scilifelab.se/researcher/90fa86e9aeaa43ea9547e48b4f3f24e3.json"}}, {"family": "Lipcsey", "given": "Mikl\u00f3s", "initials": "M", "orcid": "0000-0002-1976-4129", "researcher": {"href": "https://publications.scilifelab.se/researcher/81805f2324634628abefcf0ab6ce6a15.json"}}, {"family": "Frithiof", "given": "Robert", "initials": "R", "orcid": "0000-0003-2278-7951", "researcher": {"href": "https://publications.scilifelab.se/researcher/8fec11dd18f941b7842610ad14237a35.json"}}, {"family": "Larsson", "given": "Anders O", "initials": "AO", "orcid": "0000-0003-3161-0402", "researcher": {"href": "https://publications.scilifelab.se/researcher/2276de26382b402aa384ac231f30f156.json"}}], "type": "journal-article", "published": "2025-11-26", "journal": {"title": "Int J Mol Sci", "issn": "1422-0067", "volume": "26", "issue": "23", "pages": "11419", "issn-l": null}, "abstract": null, "doi": "10.3390/ijms262311419", "pmid": null, "labels": {"Affinity Proteomics Uppsala": "Collaborative"}, "xrefs": [], "notes": [], "created": "2025-11-26T13:51:26.247Z", "modified": "2025-11-26T13:51:37.193Z"}, {"entity": "publication", "iuid": "02f127da3ff74424b59f25012f4991b9", "links": {"self": {"href": "https://publications.scilifelab.se/publication/02f127da3ff74424b59f25012f4991b9.json"}, "display": {"href": "https://publications.scilifelab.se/publication/02f127da3ff74424b59f25012f4991b9"}}, "title": "Liquid biomarkers associate with TGF-\u03b2 Type I receptor and hypoxia in kidney cancer.", "authors": [{"family": "Mallikarjuna", "given": "Pramod", "initials": "P"}, {"family": "Erdem", "given": "Cemal", "initials": "C", "orcid": "0000-0003-3663-3646", "researcher": {"href": "https://publications.scilifelab.se/researcher/a7540b2308f548ab82104ba653ee5eb5.json"}}, {"family": "Beorlegui", "given": "Ruben Ilundain", "initials": "RI"}, {"family": "Larsson", "given": "Anders", "initials": "A", "orcid": "0000-0003-3161-0402", "researcher": {"href": "https://publications.scilifelab.se/researcher/2276de26382b402aa384ac231f30f156.json"}}, {"family": "Ljungberg", "given": "B\u00f6rje", "initials": "B"}, {"family": "Kamali-Moghaddam", "given": "Masood", "initials": "M"}, {"family": "Landstr\u00f6m", "given": "Mar\u00e9ne", "initials": "M", "orcid": "0000-0001-6737-7230", "researcher": {"href": "https://publications.scilifelab.se/researcher/c2f02fcfb1c1497d81a6f343bc0e6928.json"}}], "type": "letter", "published": "2025-09-26", "journal": {"title": "Signal Transduct Target Ther", "issn": "2059-3635", "volume": "10", "issue": "1", "pages": "309", "issn-l": null}, "abstract": null, "doi": "10.1038/s41392-025-02404-7", "pmid": "40998794", "labels": {"Affinity Proteomics Uppsala": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC12464240"}, {"db": "pii", "key": "10.1038/s41392-025-02404-7"}], "notes": [], "created": "2025-11-25T19:21:37.895Z", "modified": "2025-11-25T19:21:38.040Z"}, {"entity": "publication", "iuid": "c1a6072bf7ac4b2ab7b22b6938ed192f", "links": {"self": {"href": "https://publications.scilifelab.se/publication/c1a6072bf7ac4b2ab7b22b6938ed192f.json"}, "display": {"href": "https://publications.scilifelab.se/publication/c1a6072bf7ac4b2ab7b22b6938ed192f"}}, "title": "Significant Interplay Between Lipids, Cytokines, Chemokines, Growth Factors, and Blood Cells in an Outpatient Cohort.", "authors": [{"family": "Eriksson", "given": "Mats B", "initials": "MB", "orcid": "0000-0002-3178-4210", "researcher": {"href": "https://publications.scilifelab.se/researcher/cfcf6caaeeea422997c259d802e7e6f8.json"}}, {"family": "Eriksson", "given": "Lars B", "initials": "LB", "orcid": "0009-0002-1818-7347", "researcher": {"href": "https://publications.scilifelab.se/researcher/44d17c21be93409fb4d5bc5de121b497.json"}}, {"family": "Larsson", "given": "Anders O", "initials": "AO", "orcid": "0000-0003-3161-0402", "researcher": {"href": "https://publications.scilifelab.se/researcher/2276de26382b402aa384ac231f30f156.json"}}], "type": "journal article", "published": "2025-08-11", "journal": {"title": "Int J Mol Sci", "issn": "1422-0067", "volume": "26", "issue": "16", "issn-l": null}, "abstract": "Cardiovascular disease (CVD) remains the leading global cause of morbidity and mortality, largely driven by atherosclerosis, a chronic inflammatory process involving lipids and immune cells. Although traditional lipid biomarkers such as low-density lipoprotein (LDL) and high-density lipoprotein (HDL) are well-established in CVD risk stratification, the interplay between cytokines, chemokines, growth factors (CCGFs), lipid metabolism, and hematological parameters in non-cardiac populations remains underexplored. We investigated associations between plasma cytokines and lipid-related biomarkers and their relationships with circulating blood cell counts in a cohort of 164 essentially healthy adults aged 18-44 years. CCGF profiling was performed using a proximity extension assay (PEA), and statistical correlations were adjusted for multiple testing using false discovery rate (FDR) correction. The CCGFs that were associated with HDL and apolipoprotein A1 all displayed negative associations. Several pro-inflammatory cytokines, including CCL3, IL-6, and TNFSF10, showed strong positive associations with triglycerides, remnants, non-HDL, and body mass index (BMI). Furthermore, triglycerides and remnants were consistently correlated with elevated leukocyte, neutrophil, and platelet counts. HGF and FGF-21, mainly considered as anti-inflammatory, were positively associated with BMI and negatively associated with HDL, which is compliant with a multitude of actions, depending on the local milieu and the cellular interplay. Our results support the existence of a complex immunometabolic network involving lipids, CCGFs, and blood cells, even in non-diseased individuals. The observed patterns underscore the importance of understanding the intricate cytokine-lipid-cell interactions that may occur in early pathophysiological processes and highlight their potential utility in refining cardiovascular risk assessment beyond traditional lipid metrics.", "doi": "10.3390/ijms26167746", "pmid": "40869066", "labels": {"Affinity Proteomics Uppsala": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC12387115"}, {"db": "pii", "key": "ijms26167746"}], "notes": [], "created": "2025-11-25T19:21:46.609Z", "modified": "2025-11-25T19:21:46.738Z"}, {"entity": "publication", "iuid": "f2093e58779b4746bef0e24ae100356e", "links": {"self": {"href": "https://publications.scilifelab.se/publication/f2093e58779b4746bef0e24ae100356e.json"}, "display": {"href": "https://publications.scilifelab.se/publication/f2093e58779b4746bef0e24ae100356e"}}, "title": "Significant Associations Between Blood Cell Counts and a Large Number of Salivary Cytokines, Chemokines, and Growth Factors.", "authors": [{"family": "Eriksson", "given": "Lars B", "initials": "LB", "orcid": "0009-0002-1818-7347", "researcher": {"href": "https://publications.scilifelab.se/researcher/44d17c21be93409fb4d5bc5de121b497.json"}}, {"family": "Eriksson", "given": "Mats", "initials": "M", "orcid": "0000-0002-3178-4210", "researcher": {"href": "https://publications.scilifelab.se/researcher/cfcf6caaeeea422997c259d802e7e6f8.json"}}, {"family": "Gordh", "given": "Torsten", "initials": "T", "orcid": "0000-0003-1454-3148", "researcher": {"href": "https://publications.scilifelab.se/researcher/de40b19a854f4dda88986d2e00e8f091.json"}}, {"family": "Larsson", "given": "Anders", "initials": "A", "orcid": "0000-0003-3161-0402", "researcher": {"href": "https://publications.scilifelab.se/researcher/2276de26382b402aa384ac231f30f156.json"}}], "type": "journal article", "published": "2025-07-00", "journal": {"title": "Journal of Interferon & Cytokine Research", "issn": "1557-7465", "volume": "45", "issue": "7", "pages": "254-262", "issn-l": "1079-9907"}, "abstract": "The association between local oral inflammation and cardiovascular risk has been extensively studied, with results indicating a bidirectional relationship. The aim of the present study was to investigate the associations between blood cells and a large number of salivary cytokines, chemokines, and growth factors. The study consisted of 165 individuals who were referred to the Oral and Maxillofacial Surgery clinic at Falun County hospital, Sweden, for surgical removal of impacted lower third molar. The study subjects did not have any known inflammatory disorders. Complete blood cell counts were analyzed using the routine laboratory at Falun Hospital, Falun, Sweden. Proteomic analysis of 92 inflammation-related protein biomarkers in saliva was performed using a multiplex proximity extension assay. After adjustment for multiplicity testing using the false discovery rate approach, there remained significant association between several saliva cytokines, chemokines, and growth factors and white blood cell counts (n = 19), neutrophil counts (n = 18), erythrocyte counts (n = 13), hemoglobin concentrations (n = 20), erythrocyte volume fractions (n = 22), and platelet counts (n = 12). There are several significant associations between local inflammatory cytokines in the oral cavity and blood cell parameters indicating a relationship between local and systemic inflammatory activity.", "doi": "10.1089/jir.2025.0048", "pmid": "40392700", "labels": {"Affinity Proteomics Uppsala": "Service"}, "xrefs": [], "notes": [], "created": "2025-11-25T19:22:32.201Z", "modified": "2026-02-11T12:57:18.356Z"}, {"entity": "publication", "iuid": "b5cc99856bf64c30bfe02525fafc7f83", "links": {"self": {"href": "https://publications.scilifelab.se/publication/b5cc99856bf64c30bfe02525fafc7f83.json"}, "display": {"href": "https://publications.scilifelab.se/publication/b5cc99856bf64c30bfe02525fafc7f83"}}, "title": "Significant Associations Between Blood Cell Counts and Plasma Cytokines, Chemokines, and Growth Factors.", "authors": [{"family": "Eriksson", "given": "Lars B", "initials": "LB", "orcid": "0009-0002-1818-7347", "researcher": {"href": "https://publications.scilifelab.se/researcher/44d17c21be93409fb4d5bc5de121b497.json"}}, {"family": "Eriksson", "given": "Mats B", "initials": "MB", "orcid": "0000-0002-3178-4210", "researcher": {"href": "https://publications.scilifelab.se/researcher/cfcf6caaeeea422997c259d802e7e6f8.json"}}, {"family": "Gordh", "given": "Torsten", "initials": "T", "orcid": "0000-0003-1454-3148", "researcher": {"href": "https://publications.scilifelab.se/researcher/de40b19a854f4dda88986d2e00e8f091.json"}}, {"family": "Larsson", "given": "Anders O", "initials": "AO", "orcid": "0000-0003-3161-0402", "researcher": {"href": "https://publications.scilifelab.se/researcher/2276de26382b402aa384ac231f30f156.json"}}], "type": "journal article", "published": "2025-04-25", "journal": {"title": "Int J Mol Sci", "issn": "1422-0067", "volume": "26", "issue": "9", "pages": "4065", "issn-l": null}, "abstract": "The cytokine network plays a crucial role in regulating immune responses and facilitating intercellular communication. Cytokines are essential in numerous physiological and pathological processes. This study aimed to investigate associations between blood cell counts and a broad range of cytokines, chemokines, and growth factors. We included one hundred and sixty-five essentially healthy individuals in this study, which was approved by the Swedish Ethical Review Authority (Dnr 2015/378) and registered in EudraCT (2014-004235-39). Blood samples were collected for blood cell counts and analysis of cytokines, chemokines, and growth factors using the Proseek Multiplex Inflammation kit, Olink Bioscience, Uppsala, Sweden. Correlations between the different markers were calculated using Spearman rank correlations, adjusted for multiplicity and for multiple testing, with a significance threshold of p < 0.05. There were significant associations between platelet count and 23 cytokines, between white blood cell count and 8 cytokines, and between erythrocyte volume fractions and 19 cytokines. IL-6 had a central role within the cytokine network associated with platelets and was also associated with white blood cells and neutrophil cells. These findings emphasize the integrated nature of immune and hematological responses, where blood cell parameters are associated with systemic cytokine activity. The observed intercytokine associations, including cross-family interactions, may help to highlight regulatory pathways, providing potential targets for biomarker development and therapeutic intervention in immune-mediated conditions.", "doi": "10.3390/ijms26094065", "pmid": "40362303", "labels": {"Affinity Proteomics Uppsala": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC12072032"}, {"db": "pii", "key": "ijms26094065"}], "notes": [], "created": "2025-11-25T19:22:44.406Z", "modified": "2026-02-11T12:57:44.138Z"}, {"entity": "publication", "iuid": "866d56297cbc40a4b9f857305c123888", "links": {"self": {"href": "https://publications.scilifelab.se/publication/866d56297cbc40a4b9f857305c123888.json"}, "display": {"href": "https://publications.scilifelab.se/publication/866d56297cbc40a4b9f857305c123888"}}, "title": "Effects of denosumab treatment on the expression of receptor activator of nuclear kappa-B ligand (RANKL) and TNF-receptor TNFRSF9 after total hip arthroplasty-results from a randomized placebo-controlled clinical trial.", "authors": [{"family": "Sk\u00f6ld", "given": "C", "initials": "C", "orcid": "0000-0002-2583-5448", "researcher": {"href": "https://publications.scilifelab.se/researcher/c6b2783b9b194e66886445cbafe37bb8.json"}}, {"family": "Kultima", "given": "K", "initials": "K", "orcid": "0000-0002-0680-1410", "researcher": {"href": "https://publications.scilifelab.se/researcher/9ae376585168459681f5e2cae0c75b96.json"}}, {"family": "Freyhult", "given": "E", "initials": "E", "orcid": "0000-0003-0226-1047", "researcher": {"href": "https://publications.scilifelab.se/researcher/be110f11a53d4dcfa3bfd1657167895e.json"}}, {"family": "Larsson", "given": "A", "initials": "A", "orcid": "0000-0003-3161-0402", "researcher": {"href": "https://publications.scilifelab.se/researcher/2276de26382b402aa384ac231f30f156.json"}}, {"family": "Gordh", "given": "T", "initials": "T", "orcid": "0000-0003-1454-3148", "researcher": {"href": "https://publications.scilifelab.se/researcher/de40b19a854f4dda88986d2e00e8f091.json"}}, {"family": "Hailer", "given": "N P", "initials": "NP", "orcid": "0000-0002-3233-2638", "researcher": {"href": "https://publications.scilifelab.se/researcher/4c8bb8c013184ef7b482ffe6f8f1380b.json"}}, {"family": "Mallmin", "given": "H", "initials": "H", "orcid": "0000-0002-0074-7484", "researcher": {"href": "https://publications.scilifelab.se/researcher/bb14dfd7144c4a06970d45cbbdc0e97d.json"}}], "type": "journal article", "published": "2022-09-00", "journal": {"title": "Osteoporos Int", "issn": "1433-2965", "volume": "33", "issue": "9", "pages": "1-8", "issn-l": "0937-941X"}, "abstract": "We investigated whether the drug denosumab modulates the inflammatory response after total hip arthroplasty in a randomized controlled trial. Significantly increased expression of RANKL was found in patients treated with denosumab. This could provide an explanation for the rebound effect with rapid loss of BMD seen after discontinuation of denosumab treatment.\n\nTo evaluate whether denosumab, a human monoclonal antibody directed against receptor activator of nuclear factor kappa-B ligand (RANKL), modulates the inflammatory response after cementless total hip arthroplasty (THA) in patients with osteoarthritis of the hip.\n\nSixty-four patients operated with cementless THA were randomized to two doses of 60-mg denosumab or placebo 1-3 days and 6 months postoperatively. Serum samples were analyzed by a multiplex extension assay detecting 92 inflammation-related proteins. Bone turnover markers were assessed. Proteins were analyzed using linear mixed effect models. Validation of conspicuous findings was performed with ELISA.\n\nTwo proteins were significantly affected by denosumab treatment: RANKL and tumor necrosis factor receptor super family member 9 (TNFRSF9). Serum levels of RANKL were more than twice as high in the denosumab than in the placebo group 3 months after surgery (ratio 2.10, p<0.001). Six and 12 months after surgery, the expression of RANKL was still elevated in the denosumab-treated group (ratios 1.50, p < 0.001; 1.47, p =0.002). The expression of TNFRSF9 was lower in the denosumab group at 3 months (ratio 0.68, p<0.001). In the denosumab group, concentrations of bone turnover markers were substantially reduced after 3 months, remained suppressed after 6 and 12 months, but increased above baseline at 24 months after surgery.\n\nTwo subcutaneous denosumab injections 6 months apart increase RANKL and depress TNFRSF9 after THA. This provides a possible explanation for the rebound effect on bone turnover markers as well as bone mineral density (BMD) upon withdrawal of denosumab. None of the other measured markers of inflammation was influenced by denosumab treatment.", "doi": "10.1007/s00198-022-06423-w", "pmid": "35608639", "labels": {"Affinity Proteomics Uppsala": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC9463208"}, {"db": "pii", "key": "10.1007/s00198-022-06423-w"}], "notes": [], "created": "2022-12-02T08:48:20.134Z", "modified": "2022-12-02T08:48:20.308Z"}, {"entity": "publication", "iuid": "92002a39d15d4eb59d1072b72eb9c387", "links": {"self": {"href": "https://publications.scilifelab.se/publication/92002a39d15d4eb59d1072b72eb9c387.json"}, "display": {"href": "https://publications.scilifelab.se/publication/92002a39d15d4eb59d1072b72eb9c387"}}, "title": "Hepatocyte growth factor, colony-stimulating factor 1, CD40, and 11 other inflammation-related proteins are associated with pain in diabetic neuropathy: exploration and replication serum data from the Pain in Neuropathy Study.", "authors": [{"family": "B\u00e4ckryd", "given": "Emmanuel", "initials": "E", "orcid": "0000-0003-4420-418", "researcher": {"href": "https://publications.scilifelab.se/researcher/90b0eb424bac489e9436cc0ff3941463.json"}}, {"family": "Themistocleous", "given": "Andreas", "initials": "A", "orcid": "0000-0002-1089-1543", "researcher": {"href": "https://publications.scilifelab.se/researcher/753e444361b0448e8f28785e1af434e5.json"}}, {"family": "Larsson", "given": "Anders", "initials": "A", "orcid": "0000-0003-3161-0402", "researcher": {"href": "https://publications.scilifelab.se/researcher/2276de26382b402aa384ac231f30f156.json"}}, {"family": "Gordh", "given": "Torsten", "initials": "T"}, {"family": "Rice", "given": "Andrew S C", "initials": "ASC", "orcid": "0000-0001-9533-5636", "researcher": {"href": "https://publications.scilifelab.se/researcher/44710ccc1b084269956e6bc4b0163b3a.json"}}, {"family": "Tesfaye", "given": "Solomon", "initials": "S", "orcid": "0000-0003-1190-1472", "researcher": {"href": "https://publications.scilifelab.se/researcher/cb0fe693dd2b47c98c2681bc9d73d343.json"}}, {"family": "Bennett", "given": "David L", "initials": "DL", "orcid": "0000-0002-7996-2696", "researcher": {"href": "https://publications.scilifelab.se/researcher/0b0c988bc8344ca8b1711325ad16a85b.json"}}, {"family": "Gerdle", "given": "Bj\u00f6rn", "initials": "B", "orcid": "0000-0002-4316-1264", "researcher": {"href": "https://publications.scilifelab.se/researcher/1d3d1896af4848e2bb6054dc1c2da715.json"}}], "type": "journal article", "published": "2022-05-01", "journal": {"title": "Pain", "issn": "1872-6623", "volume": "163", "issue": "5", "pages": "897-909", "issn-l": "0304-3959"}, "abstract": "One in 5 patients with diabetes suffers from chronic pain with neuropathic characteristics, but the pathophysiological mechanisms underlying the development of neuropathic pain in patients with diabetic distal symmetrical polyneuropathy (DSP) are poorly understood. Systemic low-grade inflammation has been implicated, but there is still a considerable knowledge gap concerning its scope and meaning in this context. The aim of the study was to establish the broad inflammatory signature of painful diabetic DSP in serum samples from the Pain in Neuropathy Study, an observational cross-sectional multicentre study in which participants underwent deep phenotyping. In the present two cohorts exploration-replication study (180 participants in each cohort), serum samples from Pain in Neuropathy Study participants were analyzed with the Olink INFLAMMATION panel (Olink Bioscience, Uppsala, Sweden) that enables the simultaneous measurement of 92 inflammation-related proteins (mainly cytokines, chemokines, and growth factors). In both the exploration and the replication cohort, we identified a high-inflammation subgroup where 14 inflammation-related proteins in particular were associated with more neuropathy and higher pain intensity. The top 3 proteins were hepatocyte growth factor, colony-stimulating factor 1, and CD40 in both cohorts. In the exploratory cohort, additional clinical data were available, showing an association of inflammation with insomnia and self-reported psychological distress. Hence, this cross-sectional exploration-replication study seems to confirm that low-grade systemic inflammation is related to the severity of neuropathy and neuropathic pain in a subgroup of patients with diabetic DSP. The pathophysiological relevance of these proteins for the development of neuropathic pain in patients with diabetic DSP must be explored in more depth in future studies.", "doi": "10.1097/j.pain.0000000000002451", "pmid": "34433766", "labels": {"Affinity Proteomics Uppsala": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC9009322"}, {"db": "pii", "key": "00006396-202205000-00014"}], "notes": [], "created": "2022-12-02T09:02:32.019Z", "modified": "2022-12-02T09:02:32.203Z"}, {"entity": "publication", "iuid": "daf8eeb3e56f4feb90c6f3e80c64b485", "links": {"self": {"href": "https://publications.scilifelab.se/publication/daf8eeb3e56f4feb90c6f3e80c64b485.json"}, "display": {"href": "https://publications.scilifelab.se/publication/daf8eeb3e56f4feb90c6f3e80c64b485"}}, "title": "Periodontal Disease Augments Cardiovascular Disease Risk Biomarkers in Rheumatoid Arthritis.", "authors": [{"family": "Panezai", "given": "Jeneen", "initials": "J", "orcid": "0000-0001-5417-9788", "researcher": {"href": "https://publications.scilifelab.se/researcher/23cfa0654a5f494ab17f9542febc7e60.json"}}, {"family": "Ghaffar", "given": "Ambereen", "initials": "A"}, {"family": "Altamash", "given": "Mohammad", "initials": "M"}, {"family": "\u00c5berg", "given": "Mikael", "initials": "M"}, {"family": "Van Dyke", "given": "Thomas E", "initials": "TE"}, {"family": "Larsson", "given": "Anders", "initials": "A", "orcid": "0000-0003-3161-0402", "researcher": {"href": "https://publications.scilifelab.se/researcher/2276de26382b402aa384ac231f30f156.json"}}, {"family": "Engstr\u00f6m", "given": "Per-Erik", "initials": "PE"}], "type": "journal article", "published": "2022-03-19", "journal": {"title": "Biomedicines", "issn": "2227-9059", "volume": "10", "issue": "3", "issn-l": null}, "abstract": "Periodontal disease (PD) and rheumatoid arthritis (RA) are known chronic conditions with sustained inflammation leading to osteolysis. Cardiovascular diseases (CVD) are frequent comorbidities that may arise from sustained inflammation associated with both PD and RA. In order to determine CVD risk, alterations at the molecular level need to be identified. The objective of this study, therefore, was to assess the relationship of CVD associated biomarkers in RA patients and how it is influenced by PD.\n\nThe study consisted of patient (26 RA with PD, 21 RA without PD, 51 patients with PD only) and systemically and periodontally healthy control (n = 20) groups. Periodontal parameters bleeding on probing, probing pocket depth, and marginal bone loss were determined to characterize the patient groups. Proteomic analysis of 92 CVD-related protein biomarkers was performed using a multiplex proximity extension assay. Biomarkers were clustered using the search tool for retrieval of interacting genes (STRING) to determine protein-protein interaction (PPI) networks.\n\nRA patients with PD had higher detection levels for 47% of the measured markers (ANGPT1, BOC, CCL17, CCL3, CD4, CD84, CTRC, FGF-21, FGF-23, GLO1, HAOX1, HB-EGF, hOSCAR, HSP 27, IL16, IL-17D, IL18, IL-27, IL6, LEP, LPL, MERTK, MMP12, MMP7, NEMO, PAPPA, PAR-1, PARP-1, PD-L2, PGF, PIgR, PRELP, RAGE, SCF, SLAMF7, SRC, THBS2, THPO, TNFRSF13B, TRAIL-R2, VEGFD, VSIG2, and XCL1) as compared to RA without PD. Furthermore, a strong biological network was identified amongst these proteins (clustering coefficient = 0.52, PPI enrichment p-value &lt; 0.0001). Coefficients for protein clusters involved in CVD (0.59), metabolic (0.53), and skeletal (0.51) diseases were strongest in the PD group.\n\nPeriodontal disease augments CVD-related biomarkers in RA through shared pathological clusters, concurrently enhancing metabolic and skeletal disease protein interactions, independent of autoimmune status.", "doi": "10.3390/biomedicines10030714", "pmid": "35327515", "labels": {"Affinity Proteomics Uppsala": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC8945365"}, {"db": "pii", "key": "biomedicines10030714"}], "notes": [], "created": "2022-12-02T08:50:57.351Z", "modified": "2022-12-02T08:50:57.410Z"}, {"entity": "publication", "iuid": "fd32a79bac2c4dec8dc43e60bcd060f0", "links": {"self": {"href": "https://publications.scilifelab.se/publication/fd32a79bac2c4dec8dc43e60bcd060f0.json"}, "display": {"href": "https://publications.scilifelab.se/publication/fd32a79bac2c4dec8dc43e60bcd060f0"}}, "title": "Immune-Proteome Profiling in Classical Hodgkin Lymphoma Tumor Diagnostic Tissue.", "authors": [{"family": "Gholiha", "given": "Alex Reza", "initials": "AR", "orcid": "0000-0002-3393-1106", "researcher": {"href": "https://publications.scilifelab.se/researcher/727d46bb6742412aacdf87a38957b3e9.json"}}, {"family": "Hollander", "given": "Peter", "initials": "P", "orcid": "0000-0002-0226-5681", "researcher": {"href": "https://publications.scilifelab.se/researcher/b1c6693d3ede463eb78e6da010db601f.json"}}, {"family": "L\u00f6f", "given": "Liza", "initials": "L"}, {"family": "Larsson", "given": "Anders", "initials": "A", "orcid": "0000-0003-3161-0402", "researcher": {"href": "https://publications.scilifelab.se/researcher/2276de26382b402aa384ac231f30f156.json"}}, {"family": "Hashemi", "given": "Jamileh", "initials": "J"}, {"family": "Ulfstedt", "given": "Johan Mattsson", "initials": "JM", "orcid": "0000-0003-0682-7394", "researcher": {"href": "https://publications.scilifelab.se/researcher/d97878de79294d14b317f56f7bbf774c.json"}}, {"family": "Molin", "given": "Daniel", "initials": "D"}, {"family": "Amini", "given": "Rose-Marie", "initials": "RM"}, {"family": "Freyhult", "given": "Eva", "initials": "E", "orcid": "0000-0003-0226-1047", "researcher": {"href": "https://publications.scilifelab.se/researcher/be110f11a53d4dcfa3bfd1657167895e.json"}}, {"family": "Kamali-Moghaddam", "given": "Masood", "initials": "M"}, {"family": "Enblad", "given": "Gunilla", "initials": "G"}], "type": "journal article", "published": "2021-12-21", "journal": {"title": "Cancers (Basel)", "issn": "2072-6694", "volume": "14", "issue": "1", "pages": "9", "issn-l": "2072-6694"}, "abstract": "In classical Hodgkin Lymphoma (cHL), immunoediting via protein signaling is key to evading tumor surveillance. We aimed to identify immune-related proteins that distinguish diagnostic cHL tissues (=diagnostic tumor lysates, n = 27) from control tissues (reactive lymph node lysates, n = 30). Further, we correlated our findings with the proteome plasma profile between cHL patients (n = 26) and healthy controls (n = 27). We used the proximity extension assay (PEA) with the OlinkTM multiplex Immuno-Oncology panel, consisting of 92 proteins. Univariate, multivariate-adjusted analysis and Benjamini-Hochberg's false discovery testing (=Padj) were performed to detect significant discrepancies. Proteins distinguishing cHL cases from controls were more numerous in plasma (30 proteins) than tissue (17 proteins), all Padj < 0.05. Eight of the identified proteins in cHL tissue (PD-L1, IL-6, CCL17, CCL3, IL-13, MMP12, TNFRS4, and LAG3) were elevated in both cHL tissues and cHL plasma compared with control samples. Six proteins distinguishing cHL tissues from controls tissues were significantly correlated to PD-L1 expression in cHL tissue (IL-6, MCP-2, CCL3, CCL4, GZMB, and IFN-gamma, all p \u22640.05). In conclusion, this study introduces a distinguishing proteomic profile in cHL tissue and potential immune-related markers of pathophysiological relevance.", "doi": "10.3390/cancers14010009", "pmid": "35008176", "labels": {"Bioinformatics Support, Infrastructure and Training": "Collaborative", "Bioinformatics Support and Infrastructure": "Collaborative", "Bioinformatics (NBIS)": "Collaborative"}, "xrefs": [{"db": "pii", "key": "cancers14010009"}, {"db": "pmc", "key": "PMC8750205"}], "notes": [], "created": "2022-01-11T07:52:22.416Z", "modified": "2022-11-21T15:22:11.449Z"}, {"entity": "publication", "iuid": "2002fdbdac45459d8f6df9af6aa95163", "links": {"self": {"href": "https://publications.scilifelab.se/publication/2002fdbdac45459d8f6df9af6aa95163.json"}, "display": {"href": "https://publications.scilifelab.se/publication/2002fdbdac45459d8f6df9af6aa95163"}}, "title": "Strong Associations Between Early Tubular Damage and Urinary Cytokine, Chemokine, and Growth Factor Levels in Elderly Males and Females.", "authors": [{"family": "Fellstr\u00f6m", "given": "Bengt", "initials": "B"}, {"family": "Helmersson-Karlqvist", "given": "Johanna", "initials": "J"}, {"family": "Lind", "given": "Lars", "initials": "L"}, {"family": "Soveri", "given": "Inga", "initials": "I"}, {"family": "Thulin", "given": "M\u00e5ns", "initials": "M"}, {"family": "\u00c4rnl\u00f6v", "given": "Johan", "initials": "J"}, {"family": "Kultima", "given": "Kim", "initials": "K"}, {"family": "Larsson", "given": "Anders", "initials": "A", "orcid": "0000-0003-3161-0402", "researcher": {"href": "https://publications.scilifelab.se/researcher/2276de26382b402aa384ac231f30f156.json"}}], "type": "journal article", "published": "2021-08-00", "journal": {"title": "Journal of Interferon & Cytokine Research", "issn": "1557-7465", "volume": "41", "issue": "8", "pages": "283-290", "issn-l": "1079-9907"}, "abstract": "Acute tubular necrosis is associated with high mortality rates and it is important to develop new biomarkers for tubular damage. The aim of this study was to investigate the effect of early tubular damage on a large number of urinary cytokines, chemokines, and growth factors. We selected 90 urine samples from the Prospective Investigation of the Vasculature in Uppsala Seniors Study (41 males and 49 females). The tubular damage markers cystatin C, neutrophil gelatinase-associated lipocalin (NGAL), and kidney injury molecule-1 (KIM-1) were analyzed in the urine samples and urinary cytokine levels were analyzed with 2 multiplex assays (proximity extension assay). After adjustment for sex, body mass index, estimated glomerular filtration rate, smoking, and multiplicity testing using the false discovery rate approach, there remained 26 cytokines that correlated significantly with urine cystatin C, 27 cytokines that correlated with NGAL, and 66 cytokines that correlated with KIM-1. Tubular damage shows a strong association with urinary cytokines, chemokines, and growth factors. Our findings indicate that multiplex proteomics could be a promising new approach to explore the complex effects of tubular damage.", "doi": "10.1089/jir.2021.0065", "pmid": "34410878", "labels": {"Affinity Proteomics Uppsala": "Service"}, "xrefs": [], "notes": [], "created": "2021-12-10T10:22:58.622Z", "modified": "2021-12-10T10:22:58.639Z"}, {"entity": "publication", "iuid": "4c3dae57a3fc4ca2b5799504b9fac6d5", "links": {"self": {"href": "https://publications.scilifelab.se/publication/4c3dae57a3fc4ca2b5799504b9fac6d5.json"}, "display": {"href": "https://publications.scilifelab.se/publication/4c3dae57a3fc4ca2b5799504b9fac6d5"}}, "title": "Strong Associations between Plasma Osteopontin and Several Inflammatory Chemokines, Cytokines, and Growth Factors.", "authors": [{"family": "Larsson", "given": "Anders", "initials": "A", "orcid": "0000-0003-3161-0402", "researcher": {"href": "https://publications.scilifelab.se/researcher/2276de26382b402aa384ac231f30f156.json"}}, {"family": "Helmersson-Karlqvist", "given": "Johanna", "initials": "J"}, {"family": "Lind", "given": "Lars", "initials": "L"}, {"family": "\u00c4rnl\u00f6v", "given": "Johan", "initials": "J"}, {"family": "Feldreich", "given": "Tobias Rudholm", "initials": "TR"}], "type": "journal article", "published": "2021-07-28", "journal": {"title": "Biomedicines", "issn": "2227-9059", "volume": "9", "issue": "8", "issn-l": null}, "abstract": "Osteopontin is a member of the proinflammatory cytokine network, a complex system that involves many chemokines, cytokines, and growth factors. The aim of the present study was to study the associations between osteopontin and a large number of chemokines, cytokines, and growth factors. We analyzed plasma and urine osteopontin in 652 men from the Uppsala Longitudinal Study of Adult Men (ULSAM) study cohort and compared the levels with the levels of eighty-five chemokines, cytokines, and growth factors. We found significant associations between plasma osteopontin and 37 plasma biomarkers in a model adjusted for age, and 28 of those plasma biomarkers were significant in a model also adjusting for cardiovascular risk factors. There were no significant associations after Bonferroni adjustment between urine osteopontin and any of the studied plasma cytokine biomarkers. This study shows that circulating osteopontin participates in a protein-protein interaction network of chemokines, cytokines, and growth factors. The network contains responses, pathways, and receptor binding interactions relating to cytokines, regulation of the immune system, and also regulation of apoptosis and intracellular signal transduction.", "doi": "10.3390/biomedicines9080908", "pmid": "34440113", "labels": {"Affinity Proteomics Uppsala": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC8389577"}, {"db": "pii", "key": "biomedicines9080908"}], "notes": [], "created": "2021-12-10T09:09:54.833Z", "modified": "2022-12-02T08:56:44.717Z"}, {"entity": "publication", "iuid": "276e403f383e4335ae5db33386e90d64", "links": {"self": {"href": "https://publications.scilifelab.se/publication/276e403f383e4335ae5db33386e90d64.json"}, "display": {"href": "https://publications.scilifelab.se/publication/276e403f383e4335ae5db33386e90d64"}}, "title": "Albumin Urinary Excretion Is Associated with Increased Levels of Urinary Chemokines, Cytokines, and Growth Factors Levels in Humans.", "authors": [{"family": "Fellstr\u00f6m", "given": "Bengt", "initials": "B"}, {"family": "Helmersson-Karlqvist", "given": "Johanna", "initials": "J"}, {"family": "Lind", "given": "Lars", "initials": "L", "orcid": "0000-0003-2335-8542", "researcher": {"href": "https://publications.scilifelab.se/researcher/4c517dacca7c4ec58a3e03b59ffb4044.json"}}, {"family": "Soveri", "given": "Inga", "initials": "I", "orcid": "0000-0001-6710-6422", "researcher": {"href": "https://publications.scilifelab.se/researcher/5bd8a2785a504a1ba5d7c59dc663eb3a.json"}}, {"family": "Thulin", "given": "M\u00e5ns", "initials": "M"}, {"family": "\u00c4rnl\u00f6v", "given": "Johan", "initials": "J"}, {"family": "Kultima", "given": "Kim", "initials": "K", "orcid": "0000-0002-0680-1410", "researcher": {"href": "https://publications.scilifelab.se/researcher/9ae376585168459681f5e2cae0c75b96.json"}}, {"family": "Larsson", "given": "Anders", "initials": "A", "orcid": "0000-0003-3161-0402", "researcher": {"href": "https://publications.scilifelab.se/researcher/2276de26382b402aa384ac231f30f156.json"}}], "type": "journal article", "published": "2021-03-08", "journal": {"title": "Biomolecules", "issn": "2218-273X", "volume": "11", "issue": "3", "issn-l": null}, "abstract": "The aim of the present study was to study the associations between urine albumin excretion, and a large number of urinary chemokines, cytokines, and growth factors in a normal population. We selected 90 urine samples from individuals without CVD, diabetes, stroke or kidney disease belonging to the Prospective Investigation of the Vasculature in Uppsala Seniors Study (41 males and 49 females, all aged 75 years). Urinary cytokine levels were analyzed with two multiplex assays (proximity extension assays) and the cytokine levels were correlated with urine albumin. After adjustment for sex, body mass index (BMI), estimated glomerular filtration rate (eGFR), smoking and multiplicity testing, 11 biomarkers remained significantly associated with urine albumin: thrombospondin 2, interleukin 6, interleukin 8, hepatocyte growth factor, matrix metalloproteinase-12 (MMP-12), C-X-C motif chemokine 9, tumor necrosis factor receptor superfamily member 11B, osteoprotegerin, growth-regulated alpha protein, C-X-C motif chemokine 6, oncostatin-M (OSM) and fatty acid-binding protein, intestinal, despite large differences in molecular weights. In this study, we found associations between urinary albumin and both small and large urine proteins. Additional studies are warranted to identify cytokine patterns and potential progression markers in various renal diseases.", "doi": "10.3390/biom11030396", "pmid": "33800255", "labels": {"Affinity Proteomics Uppsala": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC8000571"}, {"db": "pii", "key": "biom11030396"}], "notes": [], "created": "2022-12-02T09:03:04.456Z", "modified": "2022-12-02T09:03:04.531Z"}, {"entity": "publication", "iuid": "358994257989494b85e108f04b2dd78d", "links": {"self": {"href": "https://publications.scilifelab.se/publication/358994257989494b85e108f04b2dd78d.json"}, "display": {"href": "https://publications.scilifelab.se/publication/358994257989494b85e108f04b2dd78d"}}, "title": "Evidence of both systemic inflammation and neuroinflammation in fibromyalgia patients, as assessed by a multiplex protein panel applied to the cerebrospinal fluid and to plasma.", "authors": [{"family": "B\u00e4ckryd", "given": "Emmanuel", "initials": "E"}, {"family": "Tanum", "given": "Lars", "initials": "L"}, {"family": "Lind", "given": "Anne-Li", "initials": "AL"}, {"family": "Larsson", "given": "Anders", "initials": "A", "orcid": "0000-0003-3161-0402", "researcher": {"href": "https://publications.scilifelab.se/researcher/2276de26382b402aa384ac231f30f156.json"}}, {"family": "Gordh", "given": "Torsten", "initials": "T"}], "type": "journal article", "published": "2017-03-03", "journal": {"title": "J Pain Res", "issn": "1178-7090", "issn-l": "1178-7090", "volume": "10", "issue": null, "pages": "515-525"}, "abstract": "In addition to central hyperexcitability and impaired top-down modulation, chronic inflammation probably plays a role in the pathophysiology of fibromyalgia (FM). Indeed, on the basis of both animal experiments and human studies involving the analysis of cytokines and other inflammation-related proteins in different body fluids, neuroinflammatory mechanisms are considered to be central to the pathophysiology of many chronic pain conditions. However, concerning FM, previous human plasma/serum and/or cerebrospinal fluid (CSF) cytokine studies have looked only at a few predetermined cytokine candidates. Instead of analyzing only a few substances at a time, we used a new multiplex protein panel enabling simultaneous analysis of 92 inflammation-related proteins. Hence, we investigated the CSF and plasma inflammatory profiles of 40 FM patients compared with CSF from healthy controls (n=10) and plasma from blood donor controls (n=46). Using multivariate data analysis by projection, we found evidence of both neuroinflammation (as assessed in CSF) and chronic systemic inflammation (as assessed in plasma). Two groups of proteins (one for CSF and one for plasma) highly discriminating between patients and controls are presented. Notably, we found high levels of CSF chemokine CX3CL1 (also known as fractalkine). In addition, previous findings concerning IL-8 in FM were replicated, in both CSF and plasma. This is the first time that such an extensive inflammatory profile has been described for FM patients. Hence, FM seems to be characterized by objective biochemical alterations, and the lingering characterization of its mechanisms as essentially idiopathic or even psychogenic should be seen as definitively outdated.", "doi": "10.2147/JPR.S128508", "pmid": "28424559", "labels": {"Clinical Biomarkers": "Service", "PLA and Single Cell Proteomics": "Service", "Affinity Proteomics Uppsala": "Service"}, "xrefs": [{"db": "pii", "key": "jpr-10-515"}, {"db": "pmc", "key": "PMC5344444"}], "notes": [], "created": "2020-01-23T15:13:40.778Z", "modified": "2023-04-14T13:56:16.599Z"}]}