{"entity": "researcher", "timestamp": "2026-08-07T19:03:07.595Z", "family": "Quintero", "given": "Ileana", "initials": "I", "orcid": "0000-0001-7040-1289", "affiliations": ["Department of Veterinary Biosciences, University of Helsinki, 00014, Helsinki, Finland.", "Department of Medical and Clinical Genetics, University of Helsinki, 00014, Helsinki, Finland.", "Folkh\u00e4lsan Research Center, 00290, Helsinki, Finland."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/208e9727fc004f10acca1e3ed3244366.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/208e9727fc004f10acca1e3ed3244366"}}, "publications": [{"entity": "publication", "iuid": "c80bf1b852f04e6292b1e46a6eda7dde", "links": {"self": {"href": "https://publications.scilifelab.se/publication/c80bf1b852f04e6292b1e46a6eda7dde.json"}, "display": {"href": "https://publications.scilifelab.se/publication/c80bf1b852f04e6292b1e46a6eda7dde"}}, "title": "Small and Large Extracellular Vesicles From Human Preovulatory Follicular Fluid Display Distinct ncRNA Cargo Profiles and Differential Effects on KGN Granulosa Cells.", "authors": [{"family": "Varik", "given": "Inge", "initials": "I", "orcid": "0000-0003-1740-1022", "researcher": {"href": "https://publications.scilifelab.se/researcher/1528edffe440442f917a1e9b8d4f7309.json"}}, {"family": "Saretok", "given": "Katariina Johanna", "initials": "KJ", "orcid": "0009-0004-7370-5463", "researcher": {"href": "https://publications.scilifelab.se/researcher/a899d8117b0744aeb4c7675aa59aa64e.json"}}, {"family": "Rosenberg", "given": "Kristine", "initials": "K", "orcid": "0009-0006-0504-2818", "researcher": {"href": "https://publications.scilifelab.se/researcher/e1bebda565f44bf897594d984ab023d2.json"}}, {"family": "Quintero", "given": "Ileana", "initials": "I", "orcid": "0000-0001-7040-1289", "researcher": {"href": "https://publications.scilifelab.se/researcher/208e9727fc004f10acca1e3ed3244366.json"}}, {"family": "Puhka", "given": "Maija", "initials": "M", "orcid": "0000-0002-6445-1017", "researcher": {"href": "https://publications.scilifelab.se/researcher/b2506b7c86d543ed9c6b7309d7eaab59.json"}}, {"family": "Volkova", "given": "Nataliia", "initials": "N", "orcid": "0000-0003-1832-6116", "researcher": {"href": "https://publications.scilifelab.se/researcher/0ba8529d8ed54a239bcaa34490cd99a3.json"}}, {"family": "Tro\u0161in", "given": "Aleksander", "initials": "A"}, {"family": "Guazzi", "given": "Paolo", "initials": "P"}, {"family": "Velthut-Meikas", "given": "Agne", "initials": "A", "orcid": "0000-0003-1927-9016", "researcher": {"href": "https://publications.scilifelab.se/researcher/3371af7256714f478d42af5cf868134d.json"}}], "type": "journal article", "published": "2025-07-00", "journal": {"title": "J Extracell Vesicles", "issn": "2001-3078", "volume": "14", "issue": "7", "pages": "e70119", "issn-l": "2001-3078"}, "abstract": "Follicular fluid extracellular vesicles (FF EVs) facilitate communication between oocytes and somatic cells within the ovarian follicle, playing a pivotal role in follicular development. This study highlights the molecular and functional distinctions between small (SEV) and large (LEV) FF EV subpopulations, revealing their specialised regulatory roles in granulosa cell (GC) biology and their consequential impact on ovarian function. Single-EV profiling uncovered distinct tetraspanin distributions, with LEVs containing a lower proportion of CD9/CD63/CD81-positive particles compared to SEVs. Fluorescent labelling confirmed uptake of both SEVs and LEVs by GCs, supporting their capacity to impact cellular behaviour. Functionally, LEVs increased testosterone production by GCs, whilst SEVs had no effect on steroid hormone secretion, suggesting a specific role for LEVs in androgen biosynthesis. Transcriptomic analysis revealed extensive SEV-induced changes in GC gene expression, affecting pathways involved in transcription, TGF-\u03b2 signalling, extracellular matrix (ECM) remodelling and cell cycle regulation. In contrast, LEVs elicited minimal transcriptional changes, primarily modulating genes associated with immune regulation and oxidative stress defence. Small RNA sequencing further revealed distinct non-coding RNA (ncRNA) profiles, with SEVs enriched in miRNAs targeting pathways critical for GC differentiation, whilst LEVs carried higher levels of piRNAs implicated in maintaining genomic stability. These findings advance our understanding of FF EV-mediated intercellular communication and underscore the importance of investigating EV subpopulations independently.", "doi": "10.1002/jev2.70119", "pmid": "40620085", "labels": {"Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC12230353"}], "notes": [], "created": "2025-11-28T10:40:26.293Z", "modified": "2025-11-28T10:40:26.691Z"}, {"entity": "publication", "iuid": "af16f7f2a5004801bdd004545bd190b9", "links": {"self": {"href": "https://publications.scilifelab.se/publication/af16f7f2a5004801bdd004545bd190b9.json"}, "display": {"href": "https://publications.scilifelab.se/publication/af16f7f2a5004801bdd004545bd190b9"}}, "title": "The DoGA consortium expression atlas of promoters and genes in 100 canine tissues.", "authors": [{"family": "H\u00f6rtenhuber", "given": "Matthias", "initials": "M", "orcid": "0000-0002-5599-5565", "researcher": {"href": "https://publications.scilifelab.se/researcher/a02296c8f90742c6a9de21e12359560c.json"}}, {"family": "Hyt\u00f6nen", "given": "Marjo K", "initials": "MK", "orcid": "0000-0003-1976-5874", "researcher": {"href": "https://publications.scilifelab.se/researcher/89e3788ab09f415f918f0bf9ed441fac.json"}}, {"family": "Mukarram", "given": "Abdul Kadir", "initials": "AK"}, {"family": "Arumilli", "given": "Meharji", "initials": "M"}, {"family": "Araujo", "given": "C\u00e9sar L", "initials": "CL"}, {"family": "Quintero", "given": "Ileana", "initials": "I", "orcid": "0000-0001-7040-1289", "researcher": {"href": "https://publications.scilifelab.se/researcher/208e9727fc004f10acca1e3ed3244366.json"}}, {"family": "Syrj\u00e4", "given": "Pernilla", "initials": "P"}, {"family": "Airas", "given": "Niina", "initials": "N"}, {"family": "Kaukonen", "given": "Maria", "initials": "M", "orcid": "0000-0002-2146-4694", "researcher": {"href": "https://publications.scilifelab.se/researcher/d4e0e0816a714e72976563c625549785.json"}}, {"family": "Ky\u00f6stil\u00e4", "given": "Kaisa", "initials": "K"}, {"family": "Niskanen", "given": "Julia", "initials": "J", "orcid": "0000-0002-2871-6890", "researcher": {"href": "https://publications.scilifelab.se/researcher/6c9070b3a4bb4b9f93f85caf587b2b98.json"}}, {"family": "Jokinen", "given": "Tarja S", "initials": "TS", "orcid": "0000-0001-6389-0109", "researcher": {"href": "https://publications.scilifelab.se/researcher/435d360e63f94f48831777352c2e1d9a.json"}}, {"family": "Mottaghitalab", "given": "Faezeh", "initials": "F", "orcid": "0000-0001-6495-6667", "researcher": {"href": "https://publications.scilifelab.se/researcher/faed0b7f48c144de81c6720b9866b50f.json"}}, {"family": "Takan", "given": "I\u015f\u0131l", "initials": "I", "orcid": "0000-0002-0269-6625", "researcher": {"href": "https://publications.scilifelab.se/researcher/f7c6daf8dc524e21a2d44de764146965.json"}}, {"family": "Salokorpi", "given": "Noora", "initials": "N"}, {"family": "Raman", "given": "Amitha", "initials": "A"}, {"family": "Stevens", "given": "Irene", "initials": "I", "orcid": "0000-0003-3823-1499", "researcher": {"href": "https://publications.scilifelab.se/researcher/90f6fa81197146ae946e2f68630c1238.json"}}, {"family": "Iivanainen", "given": "Antti", "initials": "A", "orcid": "0000-0003-1002-3225", "researcher": {"href": "https://publications.scilifelab.se/researcher/6f39943341f5457986553f672841ae04.json"}}, {"family": "Yoshihara", "given": "Masahito", "initials": "M", "orcid": "0000-0002-8915-9282", "researcher": {"href": "https://publications.scilifelab.se/researcher/4303ab7a18254bb59f8e2386a7f7fb0a.json"}}, {"family": "Gusev", "given": "Oleg", "initials": "O", "orcid": "0000-0002-6203-9758", "researcher": {"href": "https://publications.scilifelab.se/researcher/31d4a1b245d449428a6fe219c91cde24.json"}}, {"family": "Bannasch", "given": "Danika", "initials": "D", "orcid": "0000-0002-7614-7207", "researcher": {"href": "https://publications.scilifelab.se/researcher/828a952f3be54507bedcb950fbafaf12.json"}}, {"family": "Sukura", "given": "Antti", "initials": "A", "orcid": "0000-0002-8992-1695", "researcher": {"href": "https://publications.scilifelab.se/researcher/ea53fec228424344bd642575de25aec3.json"}}, {"family": "Schoenebeck", "given": "Jeffrey J", "initials": "JJ", "orcid": "0000-0003-4964-2138", "researcher": {"href": "https://publications.scilifelab.se/researcher/674777750c7144d783483e2c8f130b8b.json"}}, {"family": "DoGA Consortium", "given": "", "initials": ""}, {"family": "Ezer", "given": "Sini", "initials": "S"}, {"family": "Katayama", "given": "Shintaro", "initials": "S", "orcid": "0000-0001-7581-5157", "researcher": {"href": "https://publications.scilifelab.se/researcher/c7765acd351b40c091615258c3d751b4.json"}}, {"family": "Daub", "given": "Carsten O", "initials": "CO", "orcid": "0000-0002-3295-8729", "researcher": {"href": "https://publications.scilifelab.se/researcher/eda6a90f9d9046ada163d9843d169393.json"}}, {"family": "Kere", "given": "Juha", "initials": "J", "orcid": "0000-0003-1974-0271", "researcher": {"href": "https://publications.scilifelab.se/researcher/102085fb1c3147ceaf8dcb7651df1303.json"}}, {"family": "Lohi", "given": "Hannes", "initials": "H", "orcid": "0000-0003-1087-5532", "researcher": {"href": "https://publications.scilifelab.se/researcher/903f658d43ea414fb37ef530df3e15e4.json"}}], "type": "journal article", "published": "2024-10-21", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "15", "issue": "1", "pages": "9082", "issn-l": "2041-1723"}, "abstract": "The dog, Canis lupus familiaris, is an important model for studying human diseases. Unlike many model organisms, the dog genome has a comparatively poor functional annotation, which hampers gene discovery for development, morphology, disease, and behavior. To fill this gap, we established a comprehensive tissue biobank for both the dog and wolf samples. The biobank consists of 5485 samples representing 132 tissues from 13 dogs, 12 dog embryos, and 24 wolves. In a subset of 100 tissues from nine dogs and 12 embryos, we characterized gene expression activity for each promoter, including alternative and novel, i.e., previously not annotated, promoter regions, using the 5' targeting RNA sequencing technology STRT2-seq. We identified over 100,000 promoter region candidates in the recent canine genome assembly, CanFam4, including over 45,000 highly reproducible sites with gene expression and respective tissue enrichment levels. We provide a promoter and gene expression atlas with interactive, open data resources, including a data coordination center and genome browser track hubs. We demonstrated the applicability of Dog Genome Annotation (DoGA) data and resources using multiple examples spanning canine embryonic development, morphology and behavior, and diseases across species.", "doi": "10.1038/s41467-024-52798-1", "pmid": "39433728", "labels": {"Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC11494170"}, {"db": "pii", "key": "10.1038/s41467-024-52798-1"}], "notes": [], "created": "2025-02-28T14:14:28.582Z", "modified": "2025-02-28T14:14:29.644Z"}]}