{"entity": "researcher", "timestamp": "2026-07-15T16:23:30.074Z", "family": "Andersson", "given": "Emma R", "initials": "ER", "orcid": "0000-0002-8608-625X", "affiliations": ["Department of Cell and Molecular Biology, Karolinska Institutet, Solna, Sweden.", "Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/1c7313cdcd5f41d4a567a6c315aac3a1.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/1c7313cdcd5f41d4a567a6c315aac3a1"}}, "publications": [{"entity": "publication", "iuid": "078638bca66c4645a3fe9d948edc54ba", "links": {"self": {"href": "https://publications.scilifelab.se/publication/078638bca66c4645a3fe9d948edc54ba.json"}, "display": {"href": "https://publications.scilifelab.se/publication/078638bca66c4645a3fe9d948edc54ba"}}, "title": "Mapping effective microRNA pairing beyond the seed using abasic modifications.", "authors": [{"family": "Kosek", "given": "David M", "initials": "DM", "orcid": "0000-0002-0192-4761", "researcher": {"href": "https://publications.scilifelab.se/researcher/9c830a2538b64080ba47428cdd6c92b3.json"}}, {"family": "Petzold", "given": "Katja", "initials": "K", "orcid": "0000-0001-9470-0347", "researcher": {"href": "https://publications.scilifelab.se/researcher/946f0162cdb0411493968f363c943ad5.json"}}, {"family": "Andersson", "given": "Emma R", "initials": "ER", "orcid": "0000-0002-8608-625X", "researcher": {"href": "https://publications.scilifelab.se/researcher/1c7313cdcd5f41d4a567a6c315aac3a1.json"}}], "type": "journal article", "published": "2025-04-22", "journal": {"title": "Nucleic Acids Res.", "issn": "1362-4962", "volume": "53", "issue": "8", "issn-l": "0305-1048"}, "abstract": "MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression by base-pairing to complementary sites in messenger RNAs (mRNAs). The primary element for site recognition is the seed region (nucleotides 2-8 in the miRNA), but for a minority of sites pairing outside the seed increases efficiency, with the supplementary region (nucleotides 13-16) typically having the greatest impact. However, the structural determinants of effective pairing outside the seed are not fully understood. Here, we use abasic modified nucleotides to disrupt pairing to residues 13 and 14 of miR-34a and measure the effect of this modification compared to wild-type miR-34a on the cellular transcriptome and proteome using RNA-seq and mass spectrometry. We find that a subset of sites with predicted supplementary pairing are affected by miRNA transfection, with up to two-fold decreases in site repression at the mRNA level. We show that miR-34a 3'-pairing is sensitive to GU wobble pairs in a position-specific manner and favors bulges in the miRNA over the target. These results were validated with luciferase reporter assays. Overall, this study demonstrates a novel methodological approach for elucidating the role of specific miRNA residues in target site selection, advancing our understanding of miRNA-mediated gene regulation.", "doi": "10.1093/nar/gkaf364", "pmid": "40298108", "labels": {"Global Proteomics and Proteogenomics": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC12038393"}, {"db": "pii", "key": "8121647"}], "notes": [], "created": "2025-11-27T13:03:14.944Z", "modified": "2025-11-27T13:03:15.405Z"}, {"entity": "publication", "iuid": "5a3168f3bf534a2380eced3612fd46f3", "links": {"self": {"href": "https://publications.scilifelab.se/publication/5a3168f3bf534a2380eced3612fd46f3.json"}, "display": {"href": "https://publications.scilifelab.se/publication/5a3168f3bf534a2380eced3612fd46f3"}}, "title": "Ectoderm barcoding reveals neural and cochlear compartmentalization.", "authors": [{"family": "de Haan", "given": "Sandra", "initials": "S", "orcid": "0000-0001-9335-1149", "researcher": {"href": "https://publications.scilifelab.se/researcher/ec4f3aeba7b449a9b0973f235b26eb9d.json"}}, {"family": "He", "given": "Jingyan", "initials": "J", "orcid": "0000-0002-7405-5800", "researcher": {"href": "https://publications.scilifelab.se/researcher/01a69849650d4fd0897fb4e983824d05.json"}}, {"family": "Corbat", "given": "Agustin A", "initials": "AA", "orcid": "0000-0001-8068-3486", "researcher": {"href": "https://publications.scilifelab.se/researcher/b05e4776694041d6bd47b35b69a23304.json"}}, {"family": "Belicova", "given": "Lenka", "initials": "L", "orcid": "0000-0002-6687-630X", "researcher": {"href": "https://publications.scilifelab.se/researcher/149297e7f15e4a3281be3895d45725e3.json"}}, {"family": "Ratz", "given": "Michael", "initials": "M"}, {"family": "Vinsland", "given": "Elin", "initials": "E", "orcid": "0000-0001-9695-9192", "researcher": {"href": "https://publications.scilifelab.se/researcher/2c7bf61b0d3348d9b966a7a018c8859d.json"}}, {"family": "Fris\u00e9n", "given": "Jonas", "initials": "J", "orcid": "0000-0001-5819-458X", "researcher": {"href": "https://publications.scilifelab.se/researcher/23064ee2ac9b4c2fb1eb94e61f92148e.json"}}, {"family": "Kelley", "given": "Matthew W", "initials": "MW", "orcid": "0000-0001-7367-8697", "researcher": {"href": "https://publications.scilifelab.se/researcher/0bcfe32fbc984d0a8aee2e897db13489.json"}}, {"family": "Andersson", "given": "Emma R", "initials": "ER", "orcid": "0000-0002-8608-625X", "researcher": {"href": "https://publications.scilifelab.se/researcher/1c7313cdcd5f41d4a567a6c315aac3a1.json"}}], "type": "journal article", "published": "2025-04-04", "journal": {"title": "Science", "issn": "1095-9203", "issn-l": "0036-8075", "volume": "388", "issue": "6742", "pages": "60-68"}, "abstract": "Placodes and the neural crest are defining features of vertebrates. In this study, we investigate their lineages in mice using in utero approaches. We demonstrated that nanoinjection at embryonic day 7.5 targeted the ectoderm, including the future nervous system, placodes, and neural crest, allowing highly efficient manipulation of the future nervous system and inner ear. By using heritable DNA barcodes and high-throughput next-generation single-cell lineage tracing, we elucidated convergent differentiation pathways and identified distinct nervous system-, neural crest-, and otic placode-derived lineages. Clonal analyses identified early neural and cochlear compartmentalization, linking differentiated cell types to their progenitors or cellular siblings. This provides foundational insights for neuroscience and developmental biology.", "doi": "10.1126/science.adq9248", "pmid": "40179197", "labels": {"Bioinformatics Long-term Support WABI": "Service", "Bioinformatics (NBIS)": "Service", "Bioinformatics Support, Infrastructure and Training": "Service", "National Genomics Infrastructure": "Service", "NGI Stockholm (Genomics Production)": "Service", "NGI Short read": null, "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [], "notes": [], "created": "2025-04-15T07:21:08.071Z", "modified": "2025-11-28T10:51:53.564Z"}, {"entity": "publication", "iuid": "f281918fbaf3421a9818f3d5a12981be", "links": {"self": {"href": "https://publications.scilifelab.se/publication/f281918fbaf3421a9818f3d5a12981be.json"}, "display": {"href": "https://publications.scilifelab.se/publication/f281918fbaf3421a9818f3d5a12981be"}}, "title": "Jag1 insufficiency alters liver fibrosis via T cell and hepatocyte differentiation defects.", "authors": [{"family": "Ma\u0161ek", "given": "Jan", "initials": "J", "orcid": "0000-0003-2904-3808", "researcher": {"href": "https://publications.scilifelab.se/researcher/23cbd5352a924afcb2fa82185b237cbd.json"}}, {"family": "Filipovic", "given": "Iva", "initials": "I"}, {"family": "Van Hul", "given": "No\u00e9mi", "initials": "N", "orcid": "0000-0003-1410-8808", "researcher": {"href": "https://publications.scilifelab.se/researcher/ce1f79e0359b45c9991d14649ad4bc2b.json"}}, {"family": "Belicov\u00e1", "given": "Lenka", "initials": "L", "orcid": "0000-0002-6687-630X", "researcher": {"href": "https://publications.scilifelab.se/researcher/149297e7f15e4a3281be3895d45725e3.json"}}, {"family": "Jirou\u0161kov\u00e1", "given": "Mark\u00e9ta", "initials": "M", "orcid": "0009-0004-7173-2177", "researcher": {"href": "https://publications.scilifelab.se/researcher/2f9d163ba6b44bfc8f5e8cf3e6f87e44.json"}}, {"family": "Oliveira", "given": "Daniel V", "initials": "DV", "orcid": "0000-0003-0622-2934", "researcher": {"href": "https://publications.scilifelab.se/researcher/9a7d211d1bfb41c28c667db817d8a7fb.json"}}, {"family": "Frontino", "given": "Anna Maria", "initials": "AM"}, {"family": "Hankeova", "given": "Simona", "initials": "S"}, {"family": "He", "given": "Jingyan", "initials": "J"}, {"family": "Turetti", "given": "Fabio", "initials": "F", "orcid": "0000-0003-0440-5489", "researcher": {"href": "https://publications.scilifelab.se/researcher/3d99dc0f1b3e45439fef4a0c2fed9791.json"}}, {"family": "Iqbal", "given": "Afshan", "initials": "A", "orcid": "0009-0006-4246-9925", "researcher": {"href": "https://publications.scilifelab.se/researcher/d8e111d1327f45caa414225c7a055153.json"}}, {"family": "\u010cervenka", "given": "Igor", "initials": "I"}, {"family": "Sarnov\u00e1", "given": "Lenka", "initials": "L"}, {"family": "Verboven", "given": "Elisabeth", "initials": "E", "orcid": "0000-0003-1432-2791", "researcher": {"href": "https://publications.scilifelab.se/researcher/b760332c736f4516941c50535fd14d71.json"}}, {"family": "Brabec", "given": "Tom\u00e1\u0161", "initials": "T"}, {"family": "Bj\u00f6rkstr\u00f6m", "given": "Niklas K", "initials": "NK"}, {"family": "Gregor", "given": "Martin", "initials": "M", "orcid": "0000-0001-6841-9527", "researcher": {"href": "https://publications.scilifelab.se/researcher/46ae390647a54b0fa1ddfaeb881d1efd.json"}}, {"family": "Dobe\u0161", "given": "Jan", "initials": "J", "orcid": "0000-0003-1853-1603", "researcher": {"href": "https://publications.scilifelab.se/researcher/3587f500b8c041d5b4382df838179d6a.json"}}, {"family": "Andersson", "given": "Emma R", "initials": "ER", "orcid": "0000-0002-8608-625X", "researcher": {"href": "https://publications.scilifelab.se/researcher/1c7313cdcd5f41d4a567a6c315aac3a1.json"}}], "type": "journal article", "published": "2024-11-00", "journal": {"title": "EMBO Mol Med", "issn": "1757-4684", "volume": "16", "issue": "11", "pages": "2946-2975", "issn-l": "1757-4676"}, "abstract": "Fibrosis contributes to tissue repair, but excessive fibrosis disrupts organ function. Alagille syndrome (ALGS, caused by mutations in JAGGED1) results in liver disease and characteristic fibrosis. Here, we show that Jag1Ndr/Ndr mice, a model for ALGS, recapitulate ALGS-like fibrosis. Single-cell RNA-seq and multi-color flow cytometry of the liver revealed immature hepatocytes and paradoxically low intrahepatic T cell infiltration despite cholestasis in Jag1Ndr/Ndr mice. Thymic and splenic regulatory T cells (Tregs) were enriched and Jag1Ndr/Ndr lymphocyte immune and fibrotic capacity was tested with adoptive transfer into Rag1-/- mice, challenged with dextran sulfate sodium (DSS) or bile duct ligation (BDL). Transplanted Jag1Ndr/Ndr lymphocytes were less inflammatory with fewer activated T cells than Jag1+/+ lymphocytes in response to DSS. Cholestasis induced by BDL in Rag1-/- mice with Jag1Ndr/Ndr lymphocytes resulted in periportal Treg accumulation and three-fold less periportal fibrosis than in Rag1-/- mice with Jag1+/+ lymphocytes. Finally, the Jag1Ndr/Ndr hepatocyte expression profile and Treg overrepresentation were corroborated in patients' liver samples. Jag1-dependent hepatic and immune defects thus interact to determine the fibrotic process in ALGS.", "doi": "10.1038/s44321-024-00145-8", "pmid": "39358604", "labels": {"Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC11554675"}, {"db": "pii", "key": "10.1038/s44321-024-00145-8"}], "notes": [], "created": "2024-11-25T10:22:26.021Z", "modified": "2025-02-28T14:16:54.031Z"}, {"entity": "publication", "iuid": "c0cac7649e664f42a2dad23b77043ae7", "links": {"self": {"href": "https://publications.scilifelab.se/publication/c0cac7649e664f42a2dad23b77043ae7.json"}, "display": {"href": "https://publications.scilifelab.se/publication/c0cac7649e664f42a2dad23b77043ae7"}}, "title": "Host-pathogen interactions in the Plasmodium-infected mouse liver at spatial and single-cell resolution.", "authors": [{"family": "Hildebrandt", "given": "Franziska", "initials": "F", "orcid": "0000-0002-2673-1704", "researcher": {"href": "https://publications.scilifelab.se/researcher/4c69d2d421d64a11a719a6fd55c1977a.json"}}, {"family": "Iturritza", "given": "Miren Urrutia", "initials": "MU", "orcid": "0000-0002-3989-1803", "researcher": {"href": "https://publications.scilifelab.se/researcher/65bc5beb14634ef9af3d153c19355794.json"}}, {"family": "Zwicker", "given": "Christian", "initials": "C"}, {"family": "Vanneste", "given": "Bavo", "initials": "B"}, {"family": "Van Hul", "given": "No\u00e9mi", "initials": "N", "orcid": "0000-0003-1410-8808", "researcher": {"href": "https://publications.scilifelab.se/researcher/ce1f79e0359b45c9991d14649ad4bc2b.json"}}, {"family": "Semle", "given": "Elisa", "initials": "E"}, {"family": "Quin", "given": "Jaclyn", "initials": "J"}, {"family": "Pascini", "given": "Tales", "initials": "T"}, {"family": "Saarenp\u00e4\u00e4", "given": "Sami", "initials": "S", "orcid": "0000-0003-4731-6857", "researcher": {"href": "https://publications.scilifelab.se/researcher/ee6979cdfc0b4e4285f9d810c39bb7b7.json"}}, {"family": "He", "given": "Mengxiao", "initials": "M", "orcid": "0000-0001-5905-8467", "researcher": {"href": "https://publications.scilifelab.se/researcher/79045a6ac62b4f8ea64233619eb6bfc9.json"}}, {"family": "Andersson", "given": "Emma R", "initials": "ER", "orcid": "0000-0002-8608-625X", "researcher": {"href": "https://publications.scilifelab.se/researcher/1c7313cdcd5f41d4a567a6c315aac3a1.json"}}, {"family": "Scott", "given": "Charlotte L", "initials": "CL", "orcid": "0000-0003-4914-6580", "researcher": {"href": "https://publications.scilifelab.se/researcher/75d25f2a77f9444bb6e57ade9a1388a3.json"}}, {"family": "Vega-Rodriguez", "given": "Joel", "initials": "J", "orcid": "0000-0002-9576-0058", "researcher": {"href": "https://publications.scilifelab.se/researcher/a89fc53ed4fb4e179636b66ae8d1caa3.json"}}, {"family": "Lundeberg", "given": "Joakim", "initials": "J", "orcid": "0000-0003-4313-1601", "researcher": {"href": "https://publications.scilifelab.se/researcher/4a4e6ca0f29b4ead8569e2729481c3e0.json"}}, {"family": "Ankarklev", "given": "Johan", "initials": "J", "orcid": "0000-0003-3170-8493", "researcher": {"href": "https://publications.scilifelab.se/researcher/dcf5386930e34157bf76970b16bffc02.json"}}], "type": "journal article", "published": "2024-08-19", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "15", "issue": "1", "pages": "7105", "issn-l": "2041-1723"}, "abstract": "Upon infecting its vertebrate host, the malaria parasite initially invades the liver where it undergoes massive replication, whilst remaining clinically silent. The coordination of host responses across the complex liver tissue during malaria infection remains unexplored. Here, we perform spatial transcriptomics in combination with single-nuclei RNA sequencing over multiple time points to delineate host-pathogen interactions across Plasmodium berghei-infected liver tissues. Our data reveals significant changes in spatial gene expression in the malaria-infected tissues. These include changes related to lipid metabolism in the proximity to sites of Plasmodium infection, distinct inflammation programs between lobular zones, and regions with enrichment of different inflammatory cells, which we term 'inflammatory hotspots'. We also observe significant upregulation of genes involved in inflammation in the control liver tissues of mice injected with mosquito salivary gland components. However, this response is considerably delayed compared to that observed in P. berghei-infected mice. Our study establishes a benchmark for investigating transcriptome changes during host-parasite interactions in tissues, it provides informative insights regarding in vivo study design linked to infection and offers a useful tool for the discovery and validation of de novo intervention strategies aimed at malaria liver stage infection.", "doi": "10.1038/s41467-024-51418-2", "pmid": "39160174", "labels": {"Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC11333755"}, {"db": "pii", "key": "10.1038/s41467-024-51418-2"}], "notes": [], "created": "2024-11-25T10:17:47.545Z", "modified": "2024-11-25T10:17:47.707Z"}, {"entity": "publication", "iuid": "8cbacd9195c24dc99bee025eebec1ab8", "links": {"self": {"href": "https://publications.scilifelab.se/publication/8cbacd9195c24dc99bee025eebec1ab8.json"}, "display": {"href": "https://publications.scilifelab.se/publication/8cbacd9195c24dc99bee025eebec1ab8"}}, "title": "Spatial Transcriptomics to define transcriptional patterns of zonation and structural components in the mouse liver.", "authors": [{"family": "Hildebrandt", "given": "Franziska", "initials": "F", "orcid": "0000-0002-2673-1704", "researcher": {"href": "https://publications.scilifelab.se/researcher/4c69d2d421d64a11a719a6fd55c1977a.json"}}, {"family": "Andersson", "given": "Alma", "initials": "A"}, {"family": "Saarenp\u00e4\u00e4", "given": "Sami", "initials": "S"}, {"family": "Larsson", "given": "Ludvig", "initials": "L", "orcid": "0000-0003-4209-2911", "researcher": {"href": "https://publications.scilifelab.se/researcher/e9ffc7de05a040c48011a6ba639d5851.json"}}, {"family": "Van Hul", "given": "No\u00e9mi", "initials": "N", "orcid": "0000-0003-1410-8808", "researcher": {"href": "https://publications.scilifelab.se/researcher/ce1f79e0359b45c9991d14649ad4bc2b.json"}}, {"family": "Kanatani", "given": "Sachie", "initials": "S"}, {"family": "Masek", "given": "Jan", "initials": "J", "orcid": "0000-0003-2904-3808", "researcher": {"href": "https://publications.scilifelab.se/researcher/23cbd5352a924afcb2fa82185b237cbd.json"}}, {"family": "Ellis", "given": "Ewa", "initials": "E", "orcid": "0000-0002-3057-5337", "researcher": {"href": "https://publications.scilifelab.se/researcher/82ef7cf7903140cfbb7ff8d6f4961dde.json"}}, {"family": "Barragan", "given": "Antonio", "initials": "A", "orcid": "0000-0001-7746-9964", "researcher": {"href": "https://publications.scilifelab.se/researcher/4eefb95f00db42e3891769f384269c6c.json"}}, {"family": "Mollbrink", "given": "Annelie", "initials": "A"}, {"family": "Andersson", "given": "Emma R", "initials": "ER", "orcid": "0000-0002-8608-625X", "researcher": {"href": "https://publications.scilifelab.se/researcher/1c7313cdcd5f41d4a567a6c315aac3a1.json"}}, {"family": "Lundeberg", "given": "Joakim", "initials": "J", "orcid": "0000-0003-4313-1601", "researcher": {"href": "https://publications.scilifelab.se/researcher/4a4e6ca0f29b4ead8569e2729481c3e0.json"}}, {"family": "Ankarklev", "given": "Johan", "initials": "J", "orcid": "0000-0003-3170-8493", "researcher": {"href": "https://publications.scilifelab.se/researcher/dcf5386930e34157bf76970b16bffc02.json"}}], "type": "journal article", "published": "2021-12-02", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "12", "issue": "1", "pages": "7046", "issn-l": "2041-1723"}, "abstract": "Reconstruction of heterogeneity through single cell transcriptional profiling has greatly advanced our understanding of the spatial liver transcriptome in recent years. However, global transcriptional differences across lobular units remain elusive in physical space. Here, we apply Spatial Transcriptomics to perform transcriptomic analysis across sectioned liver tissue. We confirm that the heterogeneity in this complex tissue is predominantly determined by lobular zonation. By introducing novel computational approaches, we enable transcriptional gradient measurements between tissue structures, including several lobules in a variety of orientations. Further, our data suggests the presence of previously transcriptionally uncharacterized structures within liver tissue, contributing to the overall spatial heterogeneity of the organ. This study demonstrates how comprehensive spatial transcriptomic technologies can be used to delineate extensive spatial gene expression patterns in the liver, indicating its future impact for studies of liver function, development and regeneration as well as its potential in pre-clinical and clinical pathology.", "doi": "10.1038/s41467-021-27354-w", "pmid": "34857782", "labels": {"Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pii", "key": "10.1038/s41467-021-27354-w"}, {"db": "pmc", "key": "PMC8640072"}], "notes": [], "created": "2022-11-09T15:54:10.121Z", "modified": "2024-01-16T13:48:38.011Z"}, {"entity": "publication", "iuid": "e79e384f40e94c8494e0742190163c2f", "links": {"self": {"href": "https://publications.scilifelab.se/publication/e79e384f40e94c8494e0742190163c2f.json"}, "display": {"href": "https://publications.scilifelab.se/publication/e79e384f40e94c8494e0742190163c2f"}}, "title": "Single-cell transcriptomics of human embryos identifies multiple sympathoblast lineages with potential implications for neuroblastoma origin.", "authors": [{"family": "Kameneva", "given": "Polina", "initials": "P"}, {"family": "Artemov", "given": "Artem V", "initials": "AV"}, {"family": "Kastriti", "given": "Maria Eleni", "initials": "ME", "orcid": "0000-0002-0563-7399", "researcher": {"href": "https://publications.scilifelab.se/researcher/9e0722d8c5484a13bb37c3a3b084ff8c.json"}}, {"family": "Faure", "given": "Louis", "initials": "L", "orcid": "0000-0003-4621-586X", "researcher": {"href": "https://publications.scilifelab.se/researcher/bbf218e53c854d69a1b474a61480c33f.json"}}, {"family": "Olsen", "given": "Thale K", "initials": "TK"}, {"family": "Otte", "given": "J\u00f6rg", "initials": "J"}, {"family": "Erickson", "given": "Alek", "initials": "A"}, {"family": "Semsch", "given": "Bettina", "initials": "B"}, {"family": "Andersson", "given": "Emma R", "initials": "ER", "orcid": "0000-0002-8608-625X", "researcher": {"href": "https://publications.scilifelab.se/researcher/1c7313cdcd5f41d4a567a6c315aac3a1.json"}}, {"family": "Ratz", "given": "Michael", "initials": "M"}, {"family": "Fris\u00e9n", "given": "Jonas", "initials": "J"}, {"family": "Tischler", "given": "Arthur S", "initials": "AS"}, {"family": "de Krijger", "given": "Ronald R", "initials": "RR"}, {"family": "Bouderlique", "given": "Thibault", "initials": "T", "orcid": "0000-0002-3926-990X", "researcher": {"href": "https://publications.scilifelab.se/researcher/95261ae9454044129590906f1f686bc0.json"}}, {"family": "Akkuratova", "given": "Natalia", "initials": "N"}, {"family": "Vorontsova", "given": "Maria", "initials": "M"}, {"family": "Gusev", "given": "Oleg", "initials": "O"}, {"family": "Fried", "given": "Kaj", "initials": "K"}, {"family": "Sundstr\u00f6m", "given": "Erik", "initials": "E"}, {"family": "Mei", "given": "Shenglin", "initials": "S"}, {"family": "Kogner", "given": "Per", "initials": "P", "orcid": "0000-0002-2202-9694", "researcher": {"href": "https://publications.scilifelab.se/researcher/e963274b921a4a2c8263f509334d4e22.json"}}, {"family": "Baryawno", "given": "Ninib", "initials": "N"}, {"family": "Kharchenko", "given": "Peter V", "initials": "PV", "orcid": "0000-0002-6036-5875", "researcher": {"href": "https://publications.scilifelab.se/researcher/a67002dec1264bf2865da0017405ee9b.json"}}, {"family": "Adameyko", "given": "Igor", "initials": "I", "orcid": "0000-0001-5471-0356", "researcher": {"href": "https://publications.scilifelab.se/researcher/346f484a56cb4ad5b866b194ccd44e4f.json"}}], "type": "journal article", "published": "2021-05-00", "journal": {"title": "Nat. Genet.", "issn": "1546-1718", "issn-l": "1061-4036", "volume": "53", "issue": "5", "pages": "694-706"}, "abstract": "Characterization of the progression of cellular states during human embryogenesis can provide insights into the origin of pediatric diseases. We examined the transcriptional states of neural crest- and mesoderm-derived lineages differentiating into adrenal glands, kidneys, endothelium and hematopoietic tissue between post-conception weeks 6 and 14 of human development. Our results reveal transitions connecting the intermediate mesoderm and progenitors of organ primordia, the hematopoietic system and endothelial subtypes. Unexpectedly, by using a combination of single-cell transcriptomics and lineage tracing, we found that intra-adrenal sympathoblasts at that stage are directly derived from nerve-associated Schwann cell precursors, similarly to local chromaffin cells, whereas the majority of extra-adrenal sympathoblasts arise from the migratory neural crest. In humans, this process persists during several weeks of development within the large intra-adrenal ganglia-like structures, which may also serve as reservoirs of originating cells in neuroblastoma.", "doi": "10.1038/s41588-021-00818-x", "pmid": "33833454", "labels": {"Eukaryotic Single Cell Genomics (ESCG)": "Service", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pii", "key": "10.1038/s41588-021-00818-x"}, {"db": "pmc", "key": "PMC7610777"}, {"db": "mid", "key": "EMS118444"}], "notes": [], "created": "2021-07-09T08:50:25.531Z", "modified": "2024-01-16T13:48:39.871Z"}]}