{"entity": "researcher", "timestamp": "2026-03-12T20:12:29.495Z", "family": "Scheynius", "given": "Annika", "initials": "A", "orcid": "0000-0001-5520-990X", "affiliations": ["2Department of Clinical Science and Education, Karolinska Institutet, and Sachs' Children and Youth Hospital, S\u00f6dersjukhuset, Stockholm, Sweden.", "4Clinical Genomics, SciLifeLab, Stockholm, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/1bab97d838e044d79bd32a1c207eca96.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/1bab97d838e044d79bd32a1c207eca96"}}, "publications": [{"entity": "publication", "iuid": "63fe1025f34c4decb52dd1161a5ba1fc", "links": {"self": {"href": "https://publications.scilifelab.se/publication/63fe1025f34c4decb52dd1161a5ba1fc.json"}, "display": {"href": "https://publications.scilifelab.se/publication/63fe1025f34c4decb52dd1161a5ba1fc"}}, "title": "Longitudinal analyses of development of the immune system during the first five years of life in relation to lifestyle.", "authors": [{"family": "Olin", "given": "Axel", "initials": "A", "orcid": "0000-0002-1161-4476", "researcher": {"href": "https://publications.scilifelab.se/researcher/8dfb8efde80a4a508c93714729259aba.json"}}, {"family": "Acevedo", "given": "Nathalie", "initials": "N", "orcid": "0000-0002-5154-2964", "researcher": {"href": "https://publications.scilifelab.se/researcher/f65a7562ecb24c45b0f6af3e8ac2a55b.json"}}, {"family": "Lakshmikanth", "given": "Tadepally", "initials": "T"}, {"family": "Chen", "given": "Yang", "initials": "Y"}, {"family": "Johansson", "given": "Catharina", "initials": "C"}, {"family": "Alm", "given": "Johan", "initials": "J", "orcid": "0000-0002-9062-4479", "researcher": {"href": "https://publications.scilifelab.se/researcher/0c0405c439b04431b371be36f68f5cf2.json"}}, {"family": "Scheynius", "given": "Annika", "initials": "A", "orcid": "0000-0001-5520-990X", "researcher": {"href": "https://publications.scilifelab.se/researcher/1bab97d838e044d79bd32a1c207eca96.json"}}, {"family": "Brodin", "given": "Petter", "initials": "P"}], "type": "journal article", "published": "2022-01-29", "journal": {"title": "Allergy", "issn": "1398-9995", "issn-l": "0105-4538", "volume": null, "issue": null, "pages": null}, "abstract": "Changes in immune cell composition during the immunological window within the first years after birth are not fully understood, especially the effect that different lifestyles might have on immune cell functionality.\r\n\r\nPeripheral blood mononuclear cells from mothers and their children at birth and at two and five years were analyzed by mass cytometry. Immune cell composition and functionality was analyzed according to family lifestyle (anthroposophic and non-anthroposophic).\r\n\r\nWe found no significant differences in the proportions of major immune lineages between anthroposophic and non-anthroposophic children at each timepoint, but there were clear changes over time in the proportions of mononuclear leukocytes, especially in B cells and T lymphocytes. Phenotypic distances between cord blood and maternal blood were high at birth but decreased sharply the first two years, indicating strong phenotypic convergence with maternal cells. We found that children exhibited similar stimulation responses at birth, but subsequently segregated into two discrete functional trajectories. Trajectory 1 was associated with a decrease in tumor necrosis factor alpha (TNFa) production by CD4+ T- and NK-cells, while Trajectory 2 depicted an increase in the production of IL-2 and interferon gamma (INFg) by T-cells. In both trajectories there was an increase in IL-17A production by T-cells resulting in prominent differences at five years of age.\r\n\r\nThis exploratory study suggest that leukocyte frequencies and cell phenotypes change with age in the same way across all children, while functional development follow one of two discrete trajectories that largely segregate by family lifestyle, supporting the hypothesis that early environmental exposures imprint immune cell function which may contribute to IgE sensitization. Our results also support that the first two years are critical for the environmental exposures to imprint the immune cells. Further studies with larger sample sizes are required to validate our findings.", "doi": "10.1111/all.15232", "pmid": "35094423", "labels": {"Cellular Immunomonitoring": "Technology development"}, "xrefs": [], "notes": [], "created": "2022-01-31T06:39:33.745Z", "modified": "2023-11-28T12:43:08.070Z"}, {"entity": "publication", "iuid": "fa29854dfdb74e01917b77d3a808c4c6", "links": {"self": {"href": "https://publications.scilifelab.se/publication/fa29854dfdb74e01917b77d3a808c4c6.json"}, "display": {"href": "https://publications.scilifelab.se/publication/fa29854dfdb74e01917b77d3a808c4c6"}}, "title": "The antimicrobial protein S100A12 identified as a potential autoantigen in a subgroup of atopic dermatitis patients.", "authors": [{"family": "Mikus", "given": "Maria", "initials": "M", "orcid": "0000-0001-6560-6124", "researcher": {"href": "https://publications.scilifelab.se/researcher/f569884286e147a18003426c423ec63c.json"}}, {"family": "Johansson", "given": "Catharina", "initials": "C", "orcid": "0000-0003-1303-3795", "researcher": {"href": "https://publications.scilifelab.se/researcher/d22c4c4377ec43d899a5e49e26ab611f.json"}}, {"family": "Acevedo", "given": "Nathalie", "initials": "N"}, {"family": "Nilsson", "given": "Peter", "initials": "P", "orcid": "0000-0002-4657-8532", "researcher": {"href": "https://publications.scilifelab.se/researcher/799bcf1cf8cf451296f4535dd4ca9dc0.json"}}, {"family": "Scheynius", "given": "Annika", "initials": "A", "orcid": "0000-0001-5520-990X", "researcher": {"href": "https://publications.scilifelab.se/researcher/1bab97d838e044d79bd32a1c207eca96.json"}}], "type": "journal article", "published": "2019-01-31", "journal": {"volume": "9", "issn": "2045-7022", "issue": null, "pages": "6", "title": "Clin Transl Allergy", "issn-l": "2045-7022"}, "abstract": "Atopic dermatitis (AD) is a complex heterogeneous chronic inflammatory skin disease. Specific IgE antibodies against autoantigens have been observed in a subgroup of AD patients, however, little is known about IgG-auto-reactivity in AD. To investigate the presence of autoreactive IgG antibodies, we performed autoantibody profiling of IgG in patients with AD of different severities and in healthy controls (HC).\n\nFirst, we performed an untargeted screening in plasma samples from 40 severe AD (sAD) patients and 40 HC towards 1152 protein fragments on planar antigen microarrays. Next, based on the findings and addition of more fragments, a targeted antigen suspension bead array was designed to profile a cohort of 50 sAD patients, 123 patients with moderate AD (mAD), and 84 HC against 148 protein fragments representing 96 unique proteins.\n\nForty-nine percent of the AD patients showed increased IgG-reactivity to any of the four antigens representing keratin associated protein 17-1 (KRTAP17-1), heat shock protein family A (Hsp70) member 4 (HSPA4), S100 calcium binding proteins A12 (S100A12), and Z (S100Z). The reactivity was more frequent in the sAD patients (66%) than in those with mAD (41%), whereas only present in 25% of the HC. IgG-reactivity to S100A12, a protein including an antimicrobial peptide, was only observed in AD patients (13/173).\n\nAutoantibody profiling of IgG-reactivity using microarray technology revealed an autoantibody-based subgroup in patients with AD. The four identified autoantigens and especially S100A12 could, if characterized further, increase the understanding of different pathogenic mechanisms behind AD and thereby enable better treatment.", "doi": "10.1186/s13601-019-0240-4", "pmid": "30728947", "labels": {"Autoimmunity and Serology Profiling": "Collaborative", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pii", "key": "240"}, {"db": "pmc", "key": "PMC6354350"}], "notes": [], "created": "2019-03-15T14:32:29.742Z", "modified": "2024-01-16T13:48:44.779Z"}]}