{"entity": "researcher", "timestamp": "2026-07-19T19:11:24.164Z", "family": "Fange", "given": "David", "initials": "D", "orcid": "0009-0006-3981-7618", "affiliations": ["Dept. of Cell and Molecular Biology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden. david.fange@icm.uu.se."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/195ab3e1f94e4ad39b4aa32da6b3e217.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/195ab3e1f94e4ad39b4aa32da6b3e217"}}, "publications": [{"entity": "publication", "iuid": "87a87989503945b7842ab3f695a22318", "links": {"self": {"href": "https://publications.scilifelab.se/publication/87a87989503945b7842ab3f695a22318.json"}, "display": {"href": "https://publications.scilifelab.se/publication/87a87989503945b7842ab3f695a22318"}}, "title": "Pooled optical screening in bacteria using chromosomally expressed barcodes.", "authors": [{"family": "Soares", "given": "Ruben R G", "initials": "RRG", "orcid": "0000-0001-5958-5232", "researcher": {"href": "https://publications.scilifelab.se/researcher/88ea8e0d81284c35a271f05187289390.json"}}, {"family": "Garc\u00eda-Soriano", "given": "Daniela A", "initials": "DA", "orcid": "0000-0003-2849-8528", "researcher": {"href": "https://publications.scilifelab.se/researcher/2aeeed6de99647a1878352e0da2220af.json"}}, {"family": "Larsson", "given": "Jimmy", "initials": "J"}, {"family": "Fange", "given": "David", "initials": "D", "orcid": "0009-0006-3981-7618", "researcher": {"href": "https://publications.scilifelab.se/researcher/195ab3e1f94e4ad39b4aa32da6b3e217.json"}}, {"family": "Schirman", "given": "Dvir", "initials": "D", "orcid": "0000-0001-5175-0176", "researcher": {"href": "https://publications.scilifelab.se/researcher/02550d8122ed486fb67691b456926aed.json"}}, {"family": "Grillo", "given": "Marco", "initials": "M", "orcid": "0000-0003-2155-0645", "researcher": {"href": "https://publications.scilifelab.se/researcher/bcd8bec6567444558acbcbd1b3d28f7a.json"}}, {"family": "Kn\u00f6ppel", "given": "Anna", "initials": "A"}, {"family": "Sen", "given": "Beer Chakra", "initials": "BC"}, {"family": "Svahn", "given": "Fabian", "initials": "F", "orcid": "0000-0002-5914-520X", "researcher": {"href": "https://publications.scilifelab.se/researcher/3f015b5855854d40b853c17a31d3d632.json"}}, {"family": "Zikrin", "given": "Spartak", "initials": "S", "orcid": "0000-0002-7802-8869", "researcher": {"href": "https://publications.scilifelab.se/researcher/8a82f27ea7bf43deaded33d359270820.json"}}, {"family": "Ratz", "given": "Michael", "initials": "M", "orcid": "0000-0002-9795-8033", "researcher": {"href": "https://publications.scilifelab.se/researcher/a481899ca58a467499f56af4feb5457c.json"}}, {"family": "Nilsson", "given": "Mats", "initials": "M", "orcid": "0000-0001-9985-0387", "researcher": {"href": "https://publications.scilifelab.se/researcher/197cf8ba83ba430f9712b2f4d94dc3e5.json"}}, {"family": "Elf", "given": "Johan", "initials": "J", "orcid": "0000-0001-5522-1810", "researcher": {"href": "https://publications.scilifelab.se/researcher/e8c05ef8453040a794cb9c716f0ef8d6.json"}}], "type": "journal article", "published": "2025-06-03", "journal": {"title": "Commun Biol", "issn": "2399-3642", "volume": "8", "issue": "1", "pages": "851", "issn-l": "2399-3642"}, "abstract": "Optical pooled screening is an important tool to study dynamic phenotypes for libraries of genetically engineered cells. However, the desired engineering often requires that the barcodes used for in situ genotyping are expressed from the chromosome. This has not previously been achieved in bacteria. Here we describe a method for in situ genotyping of libraries with genomic barcodes in Escherichia coli. The method is applied to measure the intracellular maturation time of 84 red fluorescent proteins.", "doi": "10.1038/s42003-025-08268-5", "pmid": "40461651", "labels": {"Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC12134211"}, {"db": "pii", "key": "10.1038/s42003-025-08268-5"}], "notes": [], "created": "2025-11-28T10:47:35.410Z", "modified": "2025-11-28T10:47:35.689Z"}, {"entity": "publication", "iuid": "d27fb32da6774e5d88889bbc52103f9b", "links": {"self": {"href": "https://publications.scilifelab.se/publication/d27fb32da6774e5d88889bbc52103f9b.json"}, "display": {"href": "https://publications.scilifelab.se/publication/d27fb32da6774e5d88889bbc52103f9b"}}, "title": "The Escherichia coli chromosome moves to the replisome.", "authors": [{"family": "Gras", "given": "Konrad", "initials": "K"}, {"family": "Fange", "given": "David", "initials": "D", "orcid": "0009-0006-3981-7618", "researcher": {"href": "https://publications.scilifelab.se/researcher/195ab3e1f94e4ad39b4aa32da6b3e217.json"}}, {"family": "Elf", "given": "Johan", "initials": "J", "orcid": "0000-0001-5522-1810", "researcher": {"href": "https://publications.scilifelab.se/researcher/e8c05ef8453040a794cb9c716f0ef8d6.json"}}], "type": "journal article", "published": "2024-07-17", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "15", "issue": "1", "pages": "6018", "issn-l": "2041-1723"}, "abstract": "In Escherichia coli, it is debated whether the two replisomes move independently along the two chromosome arms during replication or if they remain spatially confined. Here, we use high-throughput fluorescence microscopy to simultaneously determine the location and short-time-scale (1 s) movement of the replisome and a chromosomal locus throughout the cell cycle. The assay is performed for several loci. We find that (i) the two replisomes are confined to a region of ~250 nm and ~120 nm along the cell's long and short axis, respectively, (ii) the chromosomal loci move to and through this region sequentially based on their distance from the origin of replication, and (iii) when a locus is being replicated, its short time-scale movement slows down. This behavior is the same at different growth rates. In conclusion, our data supports a model with DNA moving towards spatially confined replisomes at replication.", "doi": "10.1038/s41467-024-50047-z", "pmid": "39019870", "labels": {"Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC11255300"}, {"db": "pii", "key": "10.1038/s41467-024-50047-z"}], "notes": [], "created": "2024-11-25T10:16:58.970Z", "modified": "2024-11-25T10:16:59.056Z"}]}