{"entity": "researcher", "timestamp": "2026-08-17T01:17:40.277Z", "family": "Pereira", "given": "Gabriel Vasconcelos", "initials": "GV", "orcid": "0000-0001-7937-474X", "affiliations": ["Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI, USA."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/18369e4fc4d64ae3a25f1f36a8ba9a06.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/18369e4fc4d64ae3a25f1f36a8ba9a06"}}, "publications": [{"entity": "publication", "iuid": "f7c6424898b044a587720bf8007fa557", "links": {"self": {"href": "https://publications.scilifelab.se/publication/f7c6424898b044a587720bf8007fa557.json"}, "display": {"href": "https://publications.scilifelab.se/publication/f7c6424898b044a587720bf8007fa557"}}, "title": "Ruminococcus torques is a keystone degrader of intestinal mucin glycoprotein, releasing oligosaccharides used by Bacteroides thetaiotaomicron.", "authors": [{"family": "Schaus", "given": "Sadie R", "initials": "SR", "orcid": "0000-0002-2037-5913", "researcher": {"href": "https://publications.scilifelab.se/researcher/1b870a2ff47c4652be107e54c07206ba.json"}}, {"family": "Vasconcelos Pereira", "given": "Gabriel", "initials": "G", "orcid": "0000-0001-7937-474X", "researcher": {"href": "https://publications.scilifelab.se/researcher/18369e4fc4d64ae3a25f1f36a8ba9a06.json"}}, {"family": "Luis", "given": "Ana S", "initials": "AS"}, {"family": "Madlambayan", "given": "Emily", "initials": "E"}, {"family": "Terrapon", "given": "Nicolas", "initials": "N"}, {"family": "Ostrowski", "given": "Matthew P", "initials": "MP"}, {"family": "Jin", "given": "Chunsheng", "initials": "C"}, {"family": "Henrissat", "given": "Bernard", "initials": "B"}, {"family": "Hansson", "given": "Gunnar C", "initials": "GC"}, {"family": "Martens", "given": "Eric C", "initials": "EC", "orcid": "0000-0001-6681-2990", "researcher": {"href": "https://publications.scilifelab.se/researcher/55bba05b4f76442aacb21dfaf01c0b98.json"}}], "type": "journal article", "published": "2024-08-14", "journal": {"title": "MBio", "issn": "2150-7511", "volume": "15", "issue": "8", "pages": "e0003924", "issn-l": null}, "abstract": "Symbiotic interactions between humans and our communities of resident gut microbes (microbiota) play many roles in health and disease. Some gut bacteria utilize mucus as a nutrient source and can under certain conditions damage the protective barrier it forms, increasing disease susceptibility. We investigated how Ruminococcus torques-a known mucin degrader that has been implicated in inflammatory bowel diseases (IBDs)-degrades mucin glycoproteins or their component O-linked glycans to understand its effects on the availability of mucin-derived nutrients for other bacteria. We found that R. torques utilizes both mucin glycoproteins and released oligosaccharides from gastric and colonic mucins, degrading these substrates with a panoply of mostly constitutively expressed, secreted enzymes. Investigation of mucin oligosaccharide degradation by R. torques revealed strong \u03b1-L-fucosidase, sialidase and \u03b21,4-galactosidase activities. There was a lack of detectable sulfatase and weak \u03b21,3-galactosidase degradation, resulting in accumulation of glycans containing these structures on mucin polypeptides. While the Gram-negative symbiont, Bacteroides thetaiotaomicron grows poorly on mucin glycoproteins, we demonstrate a clear ability of R. torques to liberate products from mucins, making them accessible to B. thetaiotaomicron. This work underscores the diversity of mucin-degrading mechanisms in different bacterial species and the probability that some species are contingent on others for the ability to more fully access mucin-derived nutrients. The ability of R. torques to directly degrade a variety of mucin and mucin glycan structures and unlock released glycans for other species suggests that it is a keystone mucin degrader, which might contribute to its association with IBD.IMPORTANCEAn important facet of maintaining healthy symbiosis between host and intestinal microbes is the mucus layer, the first defense protecting the epithelium from lumenal bacteria. Some gut bacteria degrade the various components of intestinal mucins, but detailed mechanisms used by different species are still emerging. It is imperative to understand these mechanisms as they likely dictate interspecies interactions and may illuminate species associated with bacterial mucus damage and subsequent disease susceptibility. Ruminococcus torques is positively associated with IBD in multiple studies. We identified mucin glycan-degrading enzymes in R. torques and found that it shares mucin degradation products with another species of gut bacteria, Bacteroides thetaiotaomicron. Our findings underscore the importance of understanding mucin degradation mechanisms in different gut bacteria and their consequences on interspecies interactions, which may identify keystone bacteria that disproportionately affect mucus damage and could therefore be key players in effects that result from reductions in mucus integrity.", "doi": "10.1128/mbio.00039-24", "pmid": "38975756", "labels": {"Glycoproteomics and MS Proteomics": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC11323728"}], "notes": [], "created": "2024-11-27T15:32:33.882Z", "modified": "2024-11-27T15:35:25.239Z"}, {"entity": "publication", "iuid": "16a9bb7483ba4829a975ba9c67cf1cbe", "links": {"self": {"href": "https://publications.scilifelab.se/publication/16a9bb7483ba4829a975ba9c67cf1cbe.json"}, "display": {"href": "https://publications.scilifelab.se/publication/16a9bb7483ba4829a975ba9c67cf1cbe"}}, "title": "A single sulfatase is required to access colonic mucin by a gut bacterium.", "authors": [{"family": "Luis", "given": "Ana S", "initials": "AS"}, {"family": "Jin", "given": "Chunsheng", "initials": "C", "orcid": "0000-0002-0229-102X", "researcher": {"href": "https://publications.scilifelab.se/researcher/458804f3f7274b8a9efe7a90469d0e56.json"}}, {"family": "Pereira", "given": "Gabriel Vasconcelos", "initials": "GV", "orcid": "0000-0001-7937-474X", "researcher": {"href": "https://publications.scilifelab.se/researcher/18369e4fc4d64ae3a25f1f36a8ba9a06.json"}}, {"family": "Glowacki", "given": "Robert W P", "initials": "RWP"}, {"family": "Gugel", "given": "Sadie R", "initials": "SR"}, {"family": "Singh", "given": "Shaleni", "initials": "S"}, {"family": "Byrne", "given": "Dominic P", "initials": "DP"}, {"family": "Pudlo", "given": "Nicholas A", "initials": "NA"}, {"family": "London", "given": "James A", "initials": "JA"}, {"family": "Basl\u00e9", "given": "Arnaud", "initials": "A"}, {"family": "Reihill", "given": "Mark", "initials": "M", "orcid": "0000-0003-3896-9346", "researcher": {"href": "https://publications.scilifelab.se/researcher/3263be5c55014c929d3b7723f677f248.json"}}, {"family": "Oscarson", "given": "Stefan", "initials": "S", "orcid": "0000-0002-8273-4918", "researcher": {"href": "https://publications.scilifelab.se/researcher/709f6e69c27e4dc48d60502e32ee5702.json"}}, {"family": "Eyers", "given": "Patrick A", "initials": "PA", "orcid": "0000-0002-9220-2966", "researcher": {"href": "https://publications.scilifelab.se/researcher/07f88ed1e8ab4f978f801a0d304419c1.json"}}, {"family": "Czjzek", "given": "Mirjam", "initials": "M", "orcid": "0000-0002-7483-2841", "researcher": {"href": "https://publications.scilifelab.se/researcher/732f891072f4492584b366085a4b71fd.json"}}, {"family": "Michel", "given": "Gurvan", "initials": "G"}, {"family": "Barbeyron", "given": "Tristan", "initials": "T"}, {"family": "Yates", "given": "Edwin A", "initials": "EA"}, {"family": "Hansson", "given": "Gunnar C", "initials": "GC", "orcid": "0000-0002-1900-1869", "researcher": {"href": "https://publications.scilifelab.se/researcher/44b3815603154322a6dac16f2fc1c1e9.json"}}, {"family": "Karlsson", "given": "Niclas G", "initials": "NG", "orcid": "0000-0002-3045-2628", "researcher": {"href": "https://publications.scilifelab.se/researcher/62e0b7440ff1447c9611d938d185fbc7.json"}}, {"family": "Cartmell", "given": "Alan", "initials": "A", "orcid": "0000-0002-5512-249X", "researcher": {"href": "https://publications.scilifelab.se/researcher/f80d10a6ee1547febcf53093c2b884ab.json"}}, {"family": "Martens", "given": "Eric C", "initials": "EC", "orcid": "0000-0001-6681-2990", "researcher": {"href": "https://publications.scilifelab.se/researcher/55bba05b4f76442aacb21dfaf01c0b98.json"}}], "type": "journal article", "published": "2021-10-00", "journal": {"title": "Nature", "issn": "1476-4687", "volume": "598", "issue": "7880", "pages": "332-337", "issn-l": "0028-0836"}, "abstract": "Humans have co-evolved with a dense community of microbial symbionts that inhabit the lower intestine. In the colon, secreted mucus creates a barrier that separates these microorganisms from the intestinal epithelium1. Some gut bacteria are able to utilize mucin glycoproteins, the main mucus component, as a nutrient source. However, it remains unclear which bacterial enzymes initiate degradation of the complex O-glycans found in mucins. In the distal colon, these glycans are heavily sulfated, but specific sulfatases that are active on colonic mucins have not been identified. Here we show that sulfatases are essential to the utilization of distal colonic mucin O-glycans by the human gut symbiont Bacteroides thetaiotaomicron. We characterized the activity of 12 different sulfatases produced by this species, showing that they are collectively active on all known sulfate linkages in O-glycans. Crystal structures of three enzymes provide mechanistic insight into the molecular basis of substrate specificity. Unexpectedly, we found that a single sulfatase is essential for utilization of sulfated O-glycans in vitro and also has a major role in vivo. Our results provide insight into the mechanisms of mucin degradation by a prominent group of gut bacteria, an important process for both normal microbial gut colonization2 and diseases such as inflammatory bowel disease3.", "doi": "10.1038/s41586-021-03967-5", "pmid": "34616040", "labels": {"Glycoproteomics and MS Proteomics": "Collaborative"}, "xrefs": [{"db": "mid", "key": "NIHMS1805309"}, {"db": "pmc", "key": "PMC9128668"}, {"db": "pii", "key": "10.1038/s41586-021-03967-5"}], "notes": [], "created": "2021-12-09T21:41:51.821Z", "modified": "2024-01-16T13:46:30.061Z"}]}