{"entity": "researcher", "timestamp": "2026-08-09T04:15:33.526Z", "family": "Hopf", "given": "Carsten", "initials": "C", "orcid": "0000-0003-0802-6451", "affiliations": ["Center for Mass Spectrometry and Optical Spectroscopy, Mannheim University of Applied Sciences, Mannheim, Germany."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/178878399aac4cd6bf793f3720c75365.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/178878399aac4cd6bf793f3720c75365"}}, "publications": [{"entity": "publication", "iuid": "4d938430321a45eea09d9bb81bc35a1c", "links": {"self": {"href": "https://publications.scilifelab.se/publication/4d938430321a45eea09d9bb81bc35a1c.json"}, "display": {"href": "https://publications.scilifelab.se/publication/4d938430321a45eea09d9bb81bc35a1c"}}, "title": "Structural amyloid plaque polymorphism is associated with distinct lipid accumulations revealed by trapped ion mobility mass spectrometry imaging.", "authors": [{"family": "Michno", "given": "Wojciech", "initials": "W", "orcid": "0000-0002-3096-3604", "researcher": {"href": "https://publications.scilifelab.se/researcher/7b4307eb6e45426e8176337e88c9c344.json"}}, {"family": "Wehrli", "given": "Patrick M", "initials": "PM"}, {"family": "Koutarapu", "given": "Srinivas", "initials": "S"}, {"family": "Marsching", "given": "Christian", "initials": "C"}, {"family": "Minta", "given": "Karolina", "initials": "K"}, {"family": "Ge", "given": "Junyue", "initials": "J"}, {"family": "Meyer", "given": "Sven W", "initials": "SW"}, {"family": "Zetterberg", "given": "Henrik", "initials": "H", "orcid": "0000-0003-3930-4354", "researcher": {"href": "https://publications.scilifelab.se/researcher/85efee74eb4a4b38b63cf2823d204529.json"}}, {"family": "Blennow", "given": "Kaj", "initials": "K"}, {"family": "Henkel", "given": "Corinna", "initials": "C"}, {"family": "Oetjen", "given": "Janina", "initials": "J", "orcid": "0000-0002-4088-5742", "researcher": {"href": "https://publications.scilifelab.se/researcher/aa10e19731a847d1a6810778bcdee4fe.json"}}, {"family": "Hopf", "given": "Carsten", "initials": "C", "orcid": "0000-0003-0802-6451", "researcher": {"href": "https://publications.scilifelab.se/researcher/178878399aac4cd6bf793f3720c75365.json"}}, {"family": "Hanrieder", "given": "J\u00f6rg", "initials": "J", "orcid": "0000-0001-6059-198X", "researcher": {"href": "https://publications.scilifelab.se/researcher/4e65454100674f98bf8f2575093f2441.json"}}], "type": "journal article", "published": "2022-02-00", "journal": {"title": "J. Neurochem.", "issn": "1471-4159", "volume": "160", "issue": "4", "pages": "482-498", "issn-l": "0022-3042"}, "abstract": "Understanding of Alzheimer's disease (AD) pathophysiology requires molecular assessment of how key pathological factors, specifically amyloid \u03b2 (A\u03b2) plaques, influence the surrounding microenvironment. Here, neuronal lipids have been implicated in A\u03b2 plaque pathology, though the lipid microenvironment in direct proximity to A\u03b2 plaques is still not fully resolved. A further challenge is the microenvironmental molecular heterogeneity, across structurally polymorphic A\u03b2 features, such as diffuse, immature, and mature, fibrillary aggregates, whose resolution requires the integration of advanced, multimodal chemical imaging tools. Herein, we used matrix-assisted laser desorption/ionization trapped ion mobility spectrometry time-of-flight based mass spectrometry imaging (MALDI TIMS TOF MSI) in combination with hyperspectral confocal microscopy to probe the lipidomic microenvironment associated with structural polymorphism of A\u03b2 plaques in transgenic Alzheimer's disease mice (tgAPPSWE ). Using on tissue and ex situ validation, TIMS MS/MS facilitated unambiguous identification of isobaric lipid species that showed plaque pathology-associated localizations. Integrated multivariate imaging data analysis revealed multiple, A\u03b2 plaque-enriched lipid patterns for gangliosides (GM), phosphoinositols (PI), phosphoethanolamines (PE), and phosphatidic acids (PA). Conversely, sulfatides (ST), cardiolipins (CL), and polyunsaturated fatty acid (PUFA)-conjugated phosphoserines (PS), and PE were depleted at plaques. Hyperspectral amyloid imaging further delineated the unique distribution of PA and PE species to mature plaque core regions, while PI, LPI, GM2 and GM3 lipids localized to immature A\u03b2 aggregates present within the periphery of A\u03b2 plaques. Finally, we followed AD pathology-associated lipid changes over time, identifying plaque- growth and maturation to be characterized by peripheral accumulation of PI (18:0/22:6). Together, these data demonstrate the potential of multimodal imaging approaches to overcome limitations associated with conventional advanced MS imaging applications. This allowed for the differentiation of both distinct lipid components in a complex micro-environment as well as their correlation to disease-relevant amyloid plaque polymorphs. Cover Image for this issue: https://doi.org/10.1111/jnc.15390.", "doi": "10.1111/jnc.15557", "pmid": "34882796", "labels": {"Integrated Microscopy Technologies Gothenburg": "Collaborative"}, "xrefs": [], "notes": [], "created": "2023-02-16T08:24:46.768Z", "modified": "2023-02-16T08:24:46.828Z"}, {"entity": "publication", "iuid": "ac498053fd134ab2a54ce6b4b2ed366c", "links": {"self": {"href": "https://publications.scilifelab.se/publication/ac498053fd134ab2a54ce6b4b2ed366c.json"}, "display": {"href": "https://publications.scilifelab.se/publication/ac498053fd134ab2a54ce6b4b2ed366c"}}, "title": "Following spatial A\u03b2 aggregation dynamics in evolving Alzheimer's disease pathology by imaging stable isotope labeling kinetics.", "authors": [{"family": "Michno", "given": "Wojciech", "initials": "W", "orcid": "0000-0002-3096-3604", "researcher": {"href": "https://publications.scilifelab.se/researcher/7b4307eb6e45426e8176337e88c9c344.json"}}, {"family": "Stringer", "given": "Katie M", "initials": "KM", "orcid": "0000-0003-1616-2437", "researcher": {"href": "https://publications.scilifelab.se/researcher/c2638dd298ce470eb41bb45cbf1501c3.json"}}, {"family": "Enzlein", "given": "Thomas", "initials": "T", "orcid": "0000-0003-1789-4090", "researcher": {"href": "https://publications.scilifelab.se/researcher/8c32c7c836c4417182d0b04ac914e318.json"}}, {"family": "Passarelli", "given": "Melissa K", "initials": "MK", "orcid": "0000-0003-2466-1439", "researcher": {"href": "https://publications.scilifelab.se/researcher/2699b550e880407296571ccfe51a1cee.json"}}, {"family": "Escrig", "given": "Stephane", "initials": "S", "orcid": "0000-0002-7525-4000", "researcher": {"href": "https://publications.scilifelab.se/researcher/5f704fbd587f4a96b272ea9269512c89.json"}}, {"family": "Vitanova", "given": "Karina", "initials": "K", "orcid": "0000-0001-8800-4150", "researcher": {"href": "https://publications.scilifelab.se/researcher/c3c495f6ef37496fbafc13a93a8d50f3.json"}}, {"family": "Wood", "given": "Jack", "initials": "J", "orcid": "0000-0001-6049-4020", "researcher": {"href": "https://publications.scilifelab.se/researcher/a9b71cccc5da4ece9a9f57f0c46eda26.json"}}, {"family": "Blennow", "given": "Kaj", "initials": "K", "orcid": "0000-0002-1890-4193", "researcher": {"href": "https://publications.scilifelab.se/researcher/5e646be026ce42ecbfd4d62eca3f9bce.json"}}, {"family": "Zetterberg", "given": "Henrik", "initials": "H", "orcid": "0000-0003-3930-4354", "researcher": {"href": "https://publications.scilifelab.se/researcher/85efee74eb4a4b38b63cf2823d204529.json"}}, {"family": "Meibom", "given": "Anders", "initials": "A", "orcid": "0000-0002-4542-2819", "researcher": {"href": "https://publications.scilifelab.se/researcher/449ed37aa5ab48ab8b1d93e0ab0f3f9b.json"}}, {"family": "Hopf", "given": "Carsten", "initials": "C", "orcid": "0000-0003-0802-6451", "researcher": {"href": "https://publications.scilifelab.se/researcher/178878399aac4cd6bf793f3720c75365.json"}}, {"family": "Edwards", "given": "Frances A", "initials": "FA", "orcid": "0000-0001-8515-9077", "researcher": {"href": "https://publications.scilifelab.se/researcher/bdb5831ebd95409b9fc30c2410bf8ad3.json"}}, {"family": "Hanrieder", "given": "J\u00f6rg", "initials": "J", "orcid": "0000-0001-6059-198X", "researcher": {"href": "https://publications.scilifelab.se/researcher/4e65454100674f98bf8f2575093f2441.json"}}], "type": "journal article", "published": "2021-06-00", "journal": {"title": "Sci Adv", "issn": "2375-2548", "volume": "7", "issue": "25", "issn-l": "2375-2548"}, "abstract": "\u03b2-Amyloid (A\u03b2) plaque formation is the major pathological hallmark of Alzheimer's disease (AD) and constitutes a potentially critical, early inducer driving AD pathogenesis as it precedes other pathological events and cognitive symptoms by decades. It is therefore critical to understand how A\u03b2 pathology is initiated and where and when distinct A\u03b2 species aggregate. Here, we used metabolic isotope labeling in APP knock-in mice together with mass spectrometry imaging to monitor the earliest seeds of A\u03b2 deposition through ongoing plaque development. This allowed visualizing A\u03b2 aggregation dynamics within single plaques across different brain regions. We show that formation of structurally distinct plaques is associated with differential A\u03b2 peptide deposition. Specifically, A\u03b21-42 is forming an initial core structure followed by radial outgrowth and late secretion and deposition of A\u03b21-38. These data describe a detailed picture of the earliest events of precipitating amyloid pathology at scales not previously possible.NL-G-F", "doi": "10.1126/sciadv.abg4855", "pmid": "34134980", "labels": {"Integrated Microscopy Technologies Gothenburg": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC8208724"}, {"db": "pii", "key": "7/25/eabg4855"}], "notes": [], "created": "2023-02-16T08:19:47.219Z", "modified": "2023-02-16T08:19:47.531Z"}]}