{"entity": "researcher", "timestamp": "2026-07-14T04:00:45.287Z", "family": "Cvjetkovic", "given": "Aleksander", "initials": "A", "orcid": "0000-0002-9131-9791", "affiliations": ["Krefting Research Centre, Institute of Medicine at the Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/166451e5c8e54c0da297f6238c42c334.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/166451e5c8e54c0da297f6238c42c334"}}, "publications": [{"entity": "publication", "iuid": "2085b9fd009043b9976ccdc98189fe21", "links": {"self": {"href": "https://publications.scilifelab.se/publication/2085b9fd009043b9976ccdc98189fe21.json"}, "display": {"href": "https://publications.scilifelab.se/publication/2085b9fd009043b9976ccdc98189fe21"}}, "title": "Proteomic profiling of tumour tissue-derived extracellular vesicles in colon cancer.", "authors": [{"family": "Cvjetkovic", "given": "Aleksander", "initials": "A", "orcid": "0000-0002-9131-9791", "researcher": {"href": "https://publications.scilifelab.se/researcher/166451e5c8e54c0da297f6238c42c334.json"}}, {"family": "Karimi", "given": "Nasibeh", "initials": "N"}, {"family": "Crescitelli", "given": "Rossella", "initials": "R", "orcid": "0000-0002-1714-3169", "researcher": {"href": "https://publications.scilifelab.se/researcher/1d4b955f26ca4a679deb090e30d0ccf1.json"}}, {"family": "Thorsell", "given": "Annika", "initials": "A"}, {"family": "Taflin", "given": "Helena", "initials": "H"}, {"family": "L\u00e4sser", "given": "Cecilia", "initials": "C"}, {"family": "L\u00f6tvall", "given": "Jan", "initials": "J", "orcid": "0000-0001-9195-9249", "researcher": {"href": "https://publications.scilifelab.se/researcher/0cbcaf6b5698411c92e0de9e8fcf390f.json"}}], "type": "journal article", "published": "2024-02-00", "journal": {"title": "J Extracell Biol", "issn": "2768-2811", "volume": "3", "issue": "2", "pages": "e127", "issn-l": null}, "abstract": "Colon cancer is one of the most commonly occurring tumours among both women and men, and over the past decades the incidence has been on the rise. As such, the need for biomarker identification as well as an understanding of the underlying disease mechanism has never been greater. Extracellular vesicles are integral mediators of cell-to-cell communication and offer a unique opportunity to study the machinery that drives disease progression, and they also function as vectors for potential biomarkers. Tumour tissue and healthy mucosal tissue from the colons of ten patients were used to isolate tissue-resident EVs that were subsequently subjected to global quantitative proteomic analysis through LC-MS/MS. In total, more than 2000 proteins were identified, with most of the common EV markers being among them. Bioinformatics revealed a clear underrepresentation of proteins involved in energy production and cellular adhesion in tumour EVs, while proteins involved in protein biosynthesis were overrepresented. Additionally, 53 membrane proteins were found to be significantly upregulated in tumour EVs. Among them were several proteins with enzymatic functions that degrade the extracellular matrix, and three of these, Fibroblast activating factor (FAP), Cell surface hyaluronidase (CEMIP2), as well as Ephrin receptor B3 (EPHB3), were validated and found to be consistent with the global quantitative results. These stark differences in the proteomes between healthy and cancerous tissue emphasise the importance of the interstitial vesicle secretome as a major player of disease development.", "doi": "10.1002/jex2.127", "pmid": "38939898", "labels": {"Integrated Microscopy Technologies Gothenburg": "Service", "Glycoproteomics and MS Proteomics": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC11080707"}, {"db": "pii", "key": "JEX2127"}], "notes": [], "created": "2024-11-15T12:03:23.005Z", "modified": "2025-10-23T09:23:07.521Z"}, {"entity": "publication", "iuid": "0006dd06fdae4e958b22569ce4577e4e", "links": {"self": {"href": "https://publications.scilifelab.se/publication/0006dd06fdae4e958b22569ce4577e4e.json"}, "display": {"href": "https://publications.scilifelab.se/publication/0006dd06fdae4e958b22569ce4577e4e"}}, "title": "T2 and T17 cytokines alter the cargo and function of airway epithelium-derived extracellular vesicles.", "authors": [{"family": "Ax", "given": "Elisabeth", "initials": "E", "orcid": "0000-0001-7553-5059", "researcher": {"href": "https://publications.scilifelab.se/researcher/3c2961415fa942a2b18c7272da4d91e4.json"}}, {"family": "Jevnikar", "given": "Zala", "initials": "Z"}, {"family": "Cvjetkovic", "given": "Aleksander", "initials": "A", "orcid": "0000-0002-9131-9791", "researcher": {"href": "https://publications.scilifelab.se/researcher/166451e5c8e54c0da297f6238c42c334.json"}}, {"family": "Malmh\u00e4ll", "given": "Carina", "initials": "C", "orcid": "0000-0001-6696-7570", "researcher": {"href": "https://publications.scilifelab.se/researcher/11c1a836d6d64df78e044403f9cc01db.json"}}, {"family": "Olsson", "given": "Henric", "initials": "H"}, {"family": "R\u00e5dinger", "given": "Madeleine", "initials": "M", "orcid": "0000-0002-0652-7378", "researcher": {"href": "https://publications.scilifelab.se/researcher/f8e9972bdb3b4d99a77a8c1fed528e21.json"}}, {"family": "L\u00e4sser", "given": "Cecilia", "initials": "C", "orcid": "0000-0003-1279-1746", "researcher": {"href": "https://publications.scilifelab.se/researcher/e14e17d2cdb24a9f93991d83811c78b9.json"}}], "type": "journal article", "published": "2020-06-19", "journal": {"title": "Respir. Res.", "issn": "1465-993X", "volume": "21", "issue": "1", "pages": "155", "issn-l": "1465-9921"}, "abstract": "Asthma is a common and heterogeneous disease that includes subgroups characterized by type 2 (T2) or type 17 (T17) immune responses for which there is a need to identify the underlying mechanisms and biomarkers in order to develop specific therapies. These subgroups can be defined by airway epithelium gene signatures and the airway epithelium has also been implicated to play a significant role in asthma pathology. Extracellular vesicles (EVs) carry functional biomolecules and participate in cell-to-cell communication in both health and disease, properties that are likely to be involved in airway diseases such as asthma. The aim of this study was to identify stimulus-specific proteins and functionality of bronchial epithelium-derived EVs following stimulation with T2 or T17 cytokines.\n\nEVs from cytokine-stimulated (T2: IL-4 + IL-13 or T17: IL-17A + TNF\u03b1) human bronchial epithelial cells cultured at air-liquid interface (HBEC-ALI) were isolated by density cushion centrifugation and size exclusion chromatography and characterized with Western blotting and electron microscopy. Transcriptomic (cells) and proteomic (EVs) profiling was also performed.\n\nOur data shows that EVs are secreted and can be isolated from the apical side of HBEC-ALI and that cytokine stimulation increases EV release. Genes upregulated in cells stimulated with T2 or T17 cytokines were increased also on protein level in the EVs. Proteins found in T17-derived EVs were suggested to be involved in pathways related to neutrophil movement which was supported by assessing neutrophil chemotaxis ex vivo.\n\nTogether, the results suggest that epithelial EVs are involved in airway inflammation and that the EV proteome may be used for discovery of disease-specific mechanisms and signatures which may enable a precision medicine approach to the treatment of asthma.", "doi": "10.1186/s12931-020-01402-3", "pmid": "32560723", "labels": {"Integrated Microscopy Technologies Gothenburg": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC7304225"}, {"db": "pii", "key": "10.1186/s12931-020-01402-3"}], "notes": [], "created": "2023-02-16T08:02:58.189Z", "modified": "2023-02-16T08:02:58.445Z"}, {"entity": "publication", "iuid": "45731fef896f4c55814ee2668776f539", "links": {"self": {"href": "https://publications.scilifelab.se/publication/45731fef896f4c55814ee2668776f539.json"}, "display": {"href": "https://publications.scilifelab.se/publication/45731fef896f4c55814ee2668776f539"}}, "title": "Subpopulations of extracellular vesicles from human metastatic melanoma tissue identified by quantitative proteomics after optimized isolation.", "authors": [{"family": "Crescitelli", "given": "Rossella", "initials": "R", "orcid": "0000-0002-1714-3169", "researcher": {"href": "https://publications.scilifelab.se/researcher/1d4b955f26ca4a679deb090e30d0ccf1.json"}}, {"family": "L\u00e4sser", "given": "Cecilia", "initials": "C", "orcid": "0000-0003-1279-1746", "researcher": {"href": "https://publications.scilifelab.se/researcher/e14e17d2cdb24a9f93991d83811c78b9.json"}}, {"family": "Jang", "given": "Su Chul", "initials": "SC", "orcid": "0000-0003-3326-1007", "researcher": {"href": "https://publications.scilifelab.se/researcher/d7866f9f05f74587af9a4d5beec0a1b5.json"}}, {"family": "Cvjetkovic", "given": "Aleksander", "initials": "A", "orcid": "0000-0002-9131-9791", "researcher": {"href": "https://publications.scilifelab.se/researcher/166451e5c8e54c0da297f6238c42c334.json"}}, {"family": "Malmh\u00e4ll", "given": "Carina", "initials": "C"}, {"family": "Karimi", "given": "Nasibeh", "initials": "N"}, {"family": "H\u00f6\u00f6g", "given": "Johanna L", "initials": "JL", "orcid": "0000-0003-2162-3816", "researcher": {"href": "https://publications.scilifelab.se/researcher/f1eaedff964f4060ae6e69f59cad4521.json"}}, {"family": "Johansson", "given": "Iva", "initials": "I", "orcid": "0000-0003-2162-3816", "researcher": {"href": "https://publications.scilifelab.se/researcher/f1eaedff964f4060ae6e69f59cad4521.json"}}, {"family": "Fuchs", "given": "Johannes", "initials": "J"}, {"family": "Thorsell", "given": "Annika", "initials": "A"}, {"family": "Gho", "given": "Yong Song", "initials": "YS"}, {"family": "Olofsson Bagge", "given": "R", "initials": "R", "orcid": "0000-0001-5795-0355", "researcher": {"href": "https://publications.scilifelab.se/researcher/e1f8016d5e64418ca71f230e64e415ba.json"}}, {"family": "L\u00f6tvall", "given": "Jan", "initials": "J", "orcid": "0000-0001-9195-9249", "researcher": {"href": "https://publications.scilifelab.se/researcher/0cbcaf6b5698411c92e0de9e8fcf390f.json"}}], "type": "journal article", "published": "2020-02-11", "journal": {"title": "J Extracell Vesicles", "issn": "2001-3078", "volume": "9", "issue": "1", "pages": "1722433", "issn-l": "2001-3078"}, "abstract": "The majority of extracellular vesicle (EV) studies conducted to date have been performed on cell lines with little knowledge on how well these represent the characteristics of EVs in vivo. The aim of this study was to establish a method to isolate and categorize subpopulations of EVs isolated directly from tumour tissue. First we established an isolation protocol for subpopulations of EVs from metastatic melanoma tissue, which included enzymatic treatment (collagenase D and DNase). Small and large EVs were isolated with differential ultracentrifugation, and these were further separated into high and low-density (HD and LD) fractions. All EV subpopulations were then analysed in depth using electron microscopy, Bioanalyzer\u00ae, nanoparticle tracking analysis, and quantitative mass spectrometry analysis. Subpopulations of EVs with distinct size, morphology, and RNA and protein cargo could be isolated from the metastatic melanoma tissue. LD EVs showed an RNA profile with the presence of 18S and 28S ribosomal subunits. In contrast, HD EVs had RNA profiles with small or no peaks for ribosomal RNA subunits. Quantitative proteomics showed that several proteins such as flotillin-1 were enriched in both large and small LD EVs, while ADAM10 were exclusively enriched in small LD EVs. In contrast, mitofilin was enriched only in the large EVs. We conclude that enzymatic treatments improve EV isolation from dense fibrotic tissue without any apparent effect on molecular or morphological characteristics. By providing a detailed categorization of several subpopulations of EVs isolated directly from tumour tissues, we might better understand the function of EVs in tumour biology and their possible use in biomarker discovery.", "doi": "10.1080/20013078.2020.1722433", "pmid": "32128073", "labels": {"Integrated Microscopy Technologies Gothenburg": "Service", "Glycoproteomics and MS Proteomics": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC7034452"}, {"db": "pii", "key": "1722433"}], "notes": [], "created": "2023-02-16T08:05:59.349Z", "modified": "2024-01-16T13:46:30.980Z"}]}