{"entity": "researcher", "timestamp": "2026-10-05T20:45:07.361Z", "family": "Jamroskovic", "given": "Jan", "initials": "J", "orcid": "0000-0001-6871-7663", "affiliations": ["Department of Medical Biochemistry and Biophysics, Ume\u00e5 University, 90187, Ume\u00e5, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/154847e2f84f4336a07e219fac6db2f9.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/154847e2f84f4336a07e219fac6db2f9"}}, "publications": [{"entity": "publication", "iuid": "81f8017172254c37a519f7c4ef21984f", "links": {"self": {"href": "https://publications.scilifelab.se/publication/81f8017172254c37a519f7c4ef21984f.json"}, "display": {"href": "https://publications.scilifelab.se/publication/81f8017172254c37a519f7c4ef21984f"}}, "title": "The Relation Between Position and Chemical Composition of Bis-Indole Substituents Determines Their Interactions with G-Quadruplex DNA.", "authors": [{"family": "Prasad", "given": "Bagineni", "initials": "B", "orcid": "0000-0002-8498-1250", "researcher": {"href": "https://publications.scilifelab.se/researcher/6eaf800ddfd04f5aaa7b29802fe433d2.json"}}, {"family": "Das", "given": "Rabindra Nath", "initials": "RN", "orcid": "0000-0001-6347-2169", "researcher": {"href": "https://publications.scilifelab.se/researcher/fec8d8bcbe8648258cfbab86693c77c6.json"}}, {"family": "Jamroskovic", "given": "Jan", "initials": "J", "orcid": "0000-0001-6871-7663", "researcher": {"href": "https://publications.scilifelab.se/researcher/154847e2f84f4336a07e219fac6db2f9.json"}}, {"family": "Kumar", "given": "Rajendra", "initials": "R", "orcid": "0000-0002-7268-9519", "researcher": {"href": "https://publications.scilifelab.se/researcher/4ba57d44c650444aa51a030541d3bba7.json"}}, {"family": "Hedenstr\u00f6m", "given": "Mattias", "initials": "M"}, {"family": "Sabouri", "given": "Nasim", "initials": "N", "orcid": "0000-0002-4541-7702", "researcher": {"href": "https://publications.scilifelab.se/researcher/4bdc688dc85a4932acfdfffad8bfc443.json"}}, {"family": "Chorell", "given": "Erik", "initials": "E", "orcid": "0000-0003-2523-1940", "researcher": {"href": "https://publications.scilifelab.se/researcher/ada783ae0a824621a3b8e1024aae13a4.json"}}], "type": "journal article", "published": "2020-08-03", "journal": {"title": "Chemistry", "issn": "1521-3765", "volume": "26", "issue": "43", "pages": "9561-9572", "issn-l": "0947-6539"}, "abstract": "G-quadruplex (G4) DNA structures are linked to fundamental biological processes and human diseases, which has triggered the development of compounds that affect these DNA structures. However, more knowledge is needed about how small molecules interact with G4 DNA structures. This study describes the development of a new class of bis-indoles (3,3-diindolyl-methyl derivatives) and detailed studies of how they interact with G4 DNA using orthogonal assays, biophysical techniques, and computational studies. This revealed compounds that strongly bind and stabilize G4 DNA structures, and detailed binding interactions which for example, show that charge variance can play a key role in G4 DNA binding. Furthermore, the structure-activity relationships generated opened the possibilities to replace or introduce new substituents on the core structure, which is of key importance to optimize compound properties or introduce probes to further expand the possibilities of these compounds as tailored research tools to study G4 biology.", "doi": "10.1002/chem.202000579", "pmid": "32187406", "labels": {"Swedish NMR Centre": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC7497243"}], "notes": [], "created": "2020-03-30T11:43:49.969Z", "modified": "2025-10-17T13:03:56.658Z"}, {"entity": "publication", "iuid": "1472dc974e934876920eaee93787c313", "links": {"self": {"href": "https://publications.scilifelab.se/publication/1472dc974e934876920eaee93787c313.json"}, "display": {"href": "https://publications.scilifelab.se/publication/1472dc974e934876920eaee93787c313"}}, "title": "A site-specific self-assembled light-up rotor probe for selective recognition and stabilization of c-MYC G-quadruplex DNA.", "authors": [{"family": "Deiana", "given": "Marco", "initials": "M", "orcid": "0000-0002-7815-4494", "researcher": {"href": "https://publications.scilifelab.se/researcher/f9efbe74ca3940029d782d3ba645fa34.json"}}, {"family": "Chand", "given": "Karam", "initials": "K", "orcid": "0000-0001-7691-4392", "researcher": {"href": "https://publications.scilifelab.se/researcher/a20b7a82f4444b92b3ac1897e2fdb790.json"}}, {"family": "Jamroskovic", "given": "Jan", "initials": "J", "orcid": "0000-0001-6871-7663", "researcher": {"href": "https://publications.scilifelab.se/researcher/154847e2f84f4336a07e219fac6db2f9.json"}}, {"family": "Das", "given": "Rabindra Nath", "initials": "RN", "orcid": "0000-0001-6347-2169", "researcher": {"href": "https://publications.scilifelab.se/researcher/fec8d8bcbe8648258cfbab86693c77c6.json"}}, {"family": "Obi", "given": "Ikenna", "initials": "I", "orcid": "0000-0003-0364-8964", "researcher": {"href": "https://publications.scilifelab.se/researcher/e46c65e7b0e540b0a7c225d54c1502d7.json"}}, {"family": "Chorell", "given": "Erik", "initials": "E", "orcid": "0000-0003-2523-1940", "researcher": {"href": "https://publications.scilifelab.se/researcher/ada783ae0a824621a3b8e1024aae13a4.json"}}, {"family": "Sabouri", "given": "Nasim", "initials": "N", "orcid": "0000-0002-4541-7702", "researcher": {"href": "https://publications.scilifelab.se/researcher/4bdc688dc85a4932acfdfffad8bfc443.json"}}], "type": "journal article", "published": "2020-06-25", "journal": {"title": "Nanoscale", "issn": "2040-3372", "volume": "12", "issue": "24", "pages": "12950-12957", "issn-l": "2040-3364"}, "abstract": "Direct and unambiguous evidence of the formation of G-quadruplexes (G4s) in human cells have shown their implication in several key biological events and has emphasized their role as important targets for small-molecule cancer therapeutics. Here, we report on the first example of a self-assembled molecular-rotor G4-binder able to discriminate between an extensive panel of G4 and non-G4 structures and to selectively light-up (up to 64-fold), bind (nanomolar range), and stabilize the c-MYC promoter G4 DNA. In particular, association with the c-MYC G4 triggers the disassembly of its supramolecular state (disaggregation-induced emission, DIE) and induces geometrical restrictions (motion-induced change in emission, MICE) leading to a significant enhancement of its emission yield. Moreover, this optical reporter is able to selectively stabilize the c-MYC G4 and inhibit DNA synthesis. Finally, by using confocal laser-scanning microscopy (CLSM) we show the ability of this compound to localize primarily in the subnuclear G4-rich compartments of cancer cells. This work provides a benchmark for the future design and development of a new generation of smart sequence-selective supramolecular G4-binders that combine outstanding sensing and stability properties, to be utilized in anti-cancer therapy.", "doi": "10.1039/d0nr03404e", "pmid": "32525170", "labels": {"Swedish NMR Centre": "Service"}, "xrefs": [], "notes": [], "created": "2020-11-24T20:27:45.025Z", "modified": "2025-10-17T13:03:56.850Z"}, {"entity": "publication", "iuid": "4f91e593c9d848349f62c13e8004a3d6", "links": {"self": {"href": "https://publications.scilifelab.se/publication/4f91e593c9d848349f62c13e8004a3d6.json"}, "display": {"href": "https://publications.scilifelab.se/publication/4f91e593c9d848349f62c13e8004a3d6"}}, "title": "Quinazoline Ligands Induce Cancer Cell Death through Selective STAT3 Inhibition and G-Quadruplex Stabilization.", "authors": [{"family": "Jamroskovic", "given": "Jan", "initials": "J", "orcid": "0000-0001-6871-7663", "researcher": {"href": "https://publications.scilifelab.se/researcher/154847e2f84f4336a07e219fac6db2f9.json"}}, {"family": "Doimo", "given": "Mara", "initials": "M"}, {"family": "Chand", "given": "Karam", "initials": "K"}, {"family": "Obi", "given": "Ikenna", "initials": "I"}, {"family": "Kumar", "given": "Rajendra", "initials": "R", "orcid": "0000-0002-7268-9519", "researcher": {"href": "https://publications.scilifelab.se/researcher/4ba57d44c650444aa51a030541d3bba7.json"}}, {"family": "Br\u00e4nnstr\u00f6m", "given": "Kristoffer", "initials": "K"}, {"family": "Hedenstr\u00f6m", "given": "Mattias", "initials": "M"}, {"family": "Nath Das", "given": "Rabindra", "initials": "R", "orcid": "0000-0001-6347-2169", "researcher": {"href": "https://publications.scilifelab.se/researcher/fec8d8bcbe8648258cfbab86693c77c6.json"}}, {"family": "Akhunzianov", "given": "Almaz", "initials": "A"}, {"family": "Deiana", "given": "Marco", "initials": "M", "orcid": "0000-0002-7815-4494", "researcher": {"href": "https://publications.scilifelab.se/researcher/f9efbe74ca3940029d782d3ba645fa34.json"}}, {"family": "Kasho", "given": "Kazutoshi", "initials": "K"}, {"family": "Sulis Sato", "given": "Sebastian", "initials": "S"}, {"family": "Pourbozorgi", "given": "Parham L", "initials": "PL"}, {"family": "Mason", "given": "James E", "initials": "JE"}, {"family": "Medini", "given": "Paolo", "initials": "P"}, {"family": "\u00d6hlund", "given": "Daniel", "initials": "D"}, {"family": "Wanrooij", "given": "Sjoerd", "initials": "S"}, {"family": "Chorell", "given": "Erik", "initials": "E", "orcid": "0000-0003-2523-1940", "researcher": {"href": "https://publications.scilifelab.se/researcher/ada783ae0a824621a3b8e1024aae13a4.json"}}, {"family": "Sabouri", "given": "Nasim", "initials": "N", "orcid": "0000-0002-4541-7702", "researcher": {"href": "https://publications.scilifelab.se/researcher/4bdc688dc85a4932acfdfffad8bfc443.json"}}], "type": "journal article", "published": "2020-02-12", "journal": {"volume": "142", "issn": "1520-5126", "issue": "6", "pages": "2876-2888", "title": "J. Am. Chem. Soc.", "issn-l": "0002-7863"}, "abstract": "The signal transducer and activator of transcription 3 (STAT3) protein is a master regulator of most key hallmarks and enablers of cancer, including cell proliferation and the response to DNA damage. G-Quadruplex (G4) structures are four-stranded noncanonical DNA structures enriched at telomeres and oncogenes' promoters. In cancer cells, stabilization of G4 DNAs leads to replication stress and DNA damage accumulation and is therefore considered a promising target for oncotherapy. Here, we designed and synthesized novel quinazoline-based compounds that simultaneously and selectively affect these two well-recognized cancer targets, G4 DNA structures and the STAT3 protein. Using a combination of in vitro assays, NMR, and molecular dynamics simulations, we show that these small, uncharged compounds not only bind to the STAT3 protein but also stabilize G4 structures. In human cultured cells, the compounds inhibit phosphorylation-dependent activation of STAT3 without affecting the antiapoptotic factor STAT1 and cause increased formation of G4 structures, as revealed by the use of a G4 DNA-specific antibody. As a result, treated cells show slower DNA replication, DNA damage checkpoint activation, and an increased apoptotic rate. Importantly, cancer cells are more sensitive to these molecules compared to noncancerous cell lines. This is the first report of a promising class of compounds that not only targets the DNA damage cancer response machinery but also simultaneously inhibits the STAT3-induced cancer cell proliferation, demonstrating a novel approach in cancer therapy.", "doi": "10.1021/jacs.9b11232", "pmid": "31990532", "labels": {"Swedish NMR Centre": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC7307907"}], "notes": [], "created": "2020-01-31T14:38:39.014Z", "modified": "2025-10-17T13:03:57.141Z"}, {"entity": "publication", "iuid": "b4b16d84e520437f8a5e4a67009681c7", "links": {"self": {"href": "https://publications.scilifelab.se/publication/b4b16d84e520437f8a5e4a67009681c7.json"}, "display": {"href": "https://publications.scilifelab.se/publication/b4b16d84e520437f8a5e4a67009681c7"}}, "title": "A Light-up Logic Platform for Selective Recognition of Parallel G-Quadruplex Structures via Disaggregation-Induced Emission.", "authors": [{"family": "Deiana", "given": "Marco", "initials": "M", "orcid": "0000-0002-7815-4494", "researcher": {"href": "https://publications.scilifelab.se/researcher/f9efbe74ca3940029d782d3ba645fa34.json"}}, {"family": "Chand", "given": "Karam", "initials": "K", "orcid": "0000-0001-7691-4392", "researcher": {"href": "https://publications.scilifelab.se/researcher/a20b7a82f4444b92b3ac1897e2fdb790.json"}}, {"family": "Jamroskovic", "given": "Jan", "initials": "J", "orcid": "0000-0001-6871-7663", "researcher": {"href": "https://publications.scilifelab.se/researcher/154847e2f84f4336a07e219fac6db2f9.json"}}, {"family": "Obi", "given": "Ikenna", "initials": "I", "orcid": "0000-0003-0364-8964", "researcher": {"href": "https://publications.scilifelab.se/researcher/e46c65e7b0e540b0a7c225d54c1502d7.json"}}, {"family": "Chorell", "given": "Erik", "initials": "E", "orcid": "0000-0003-2523-1940", "researcher": {"href": "https://publications.scilifelab.se/researcher/ada783ae0a824621a3b8e1024aae13a4.json"}}, {"family": "Sabouri", "given": "Nasim", "initials": "N", "orcid": "0000-0002-4541-7702", "researcher": {"href": "https://publications.scilifelab.se/researcher/4bdc688dc85a4932acfdfffad8bfc443.json"}}], "type": "journal article", "published": "2020-01-07", "journal": {"volume": "59", "issn": "1521-3773", "issue": "2", "pages": "896-902", "title": "Angew. Chem. Int. Ed. Engl.", "issn-l": "1433-7851"}, "abstract": "The design of turn-on dyes with optical signals sensitive to the formation of supramolecular structures provides fascinating and underexplored opportunities for G-quadruplex (G4) DNA detection and characterization. Here, we show a new switching mechanism that relies on the recognition-driven disaggregation (on-signal) of an ultrabright coumarin-quinazoline conjugate. The synthesized probe selectively lights-up parallel G4 DNA structures via the disassembly of its supramolecular state, demonstrating outputs that are easily integrable into a label-free molecular logic system. Finally, our molecule preferentially stains the G4-rich nucleoli of cancer cells.", "doi": "10.1002/anie.201912027", "pmid": "31644837", "labels": {"Integrated Microscopy Technologies Ume\u00e5": "Service"}, "xrefs": [], "notes": [], "created": "2020-02-12T11:06:01.547Z", "modified": "2021-11-10T12:55:03.771Z"}]}