{"entity": "researcher", "timestamp": "2026-07-14T03:27:24.283Z", "family": "Belicov\u00e1", "given": "Lenka", "initials": "L", "orcid": "0000-0002-6687-630X", "affiliations": ["Department of Cell and Molecular Biology, Karolinska Institute, SE-171 77 Solna, Stockholm, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/149297e7f15e4a3281be3895d45725e3.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/149297e7f15e4a3281be3895d45725e3"}}, "publications": [{"entity": "publication", "iuid": "5a3168f3bf534a2380eced3612fd46f3", "links": {"self": {"href": "https://publications.scilifelab.se/publication/5a3168f3bf534a2380eced3612fd46f3.json"}, "display": {"href": "https://publications.scilifelab.se/publication/5a3168f3bf534a2380eced3612fd46f3"}}, "title": "Ectoderm barcoding reveals neural and cochlear compartmentalization.", "authors": [{"family": "de Haan", "given": "Sandra", "initials": "S", "orcid": "0000-0001-9335-1149", "researcher": {"href": "https://publications.scilifelab.se/researcher/ec4f3aeba7b449a9b0973f235b26eb9d.json"}}, {"family": "He", "given": "Jingyan", "initials": "J", "orcid": "0000-0002-7405-5800", "researcher": {"href": "https://publications.scilifelab.se/researcher/01a69849650d4fd0897fb4e983824d05.json"}}, {"family": "Corbat", "given": "Agustin A", "initials": "AA", "orcid": "0000-0001-8068-3486", "researcher": {"href": "https://publications.scilifelab.se/researcher/b05e4776694041d6bd47b35b69a23304.json"}}, {"family": "Belicova", "given": "Lenka", "initials": "L", "orcid": "0000-0002-6687-630X", "researcher": {"href": "https://publications.scilifelab.se/researcher/149297e7f15e4a3281be3895d45725e3.json"}}, {"family": "Ratz", "given": "Michael", "initials": "M"}, {"family": "Vinsland", "given": "Elin", "initials": "E", "orcid": "0000-0001-9695-9192", "researcher": {"href": "https://publications.scilifelab.se/researcher/2c7bf61b0d3348d9b966a7a018c8859d.json"}}, {"family": "Fris\u00e9n", "given": "Jonas", "initials": "J", "orcid": "0000-0001-5819-458X", "researcher": {"href": "https://publications.scilifelab.se/researcher/23064ee2ac9b4c2fb1eb94e61f92148e.json"}}, {"family": "Kelley", "given": "Matthew W", "initials": "MW", "orcid": "0000-0001-7367-8697", "researcher": {"href": "https://publications.scilifelab.se/researcher/0bcfe32fbc984d0a8aee2e897db13489.json"}}, {"family": "Andersson", "given": "Emma R", "initials": "ER", "orcid": "0000-0002-8608-625X", "researcher": {"href": "https://publications.scilifelab.se/researcher/1c7313cdcd5f41d4a567a6c315aac3a1.json"}}], "type": "journal article", "published": "2025-04-04", "journal": {"title": "Science", "issn": "1095-9203", "issn-l": "0036-8075", "volume": "388", "issue": "6742", "pages": "60-68"}, "abstract": "Placodes and the neural crest are defining features of vertebrates. In this study, we investigate their lineages in mice using in utero approaches. We demonstrated that nanoinjection at embryonic day 7.5 targeted the ectoderm, including the future nervous system, placodes, and neural crest, allowing highly efficient manipulation of the future nervous system and inner ear. By using heritable DNA barcodes and high-throughput next-generation single-cell lineage tracing, we elucidated convergent differentiation pathways and identified distinct nervous system-, neural crest-, and otic placode-derived lineages. Clonal analyses identified early neural and cochlear compartmentalization, linking differentiated cell types to their progenitors or cellular siblings. This provides foundational insights for neuroscience and developmental biology.", "doi": "10.1126/science.adq9248", "pmid": "40179197", "labels": {"Bioinformatics Long-term Support WABI": "Service", "Bioinformatics (NBIS)": "Service", "Bioinformatics Support, Infrastructure and Training": "Service", "National Genomics Infrastructure": "Service", "NGI Stockholm (Genomics Production)": "Service", "NGI Short read": null, "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [], "notes": [], "created": "2025-04-15T07:21:08.071Z", "modified": "2025-11-28T10:51:53.564Z"}, {"entity": "publication", "iuid": "f281918fbaf3421a9818f3d5a12981be", "links": {"self": {"href": "https://publications.scilifelab.se/publication/f281918fbaf3421a9818f3d5a12981be.json"}, "display": {"href": "https://publications.scilifelab.se/publication/f281918fbaf3421a9818f3d5a12981be"}}, "title": "Jag1 insufficiency alters liver fibrosis via T cell and hepatocyte differentiation defects.", "authors": [{"family": "Ma\u0161ek", "given": "Jan", "initials": "J", "orcid": "0000-0003-2904-3808", "researcher": {"href": "https://publications.scilifelab.se/researcher/23cbd5352a924afcb2fa82185b237cbd.json"}}, {"family": "Filipovic", "given": "Iva", "initials": "I"}, {"family": "Van Hul", "given": "No\u00e9mi", "initials": "N", "orcid": "0000-0003-1410-8808", "researcher": {"href": "https://publications.scilifelab.se/researcher/ce1f79e0359b45c9991d14649ad4bc2b.json"}}, {"family": "Belicov\u00e1", "given": "Lenka", "initials": "L", "orcid": "0000-0002-6687-630X", "researcher": {"href": "https://publications.scilifelab.se/researcher/149297e7f15e4a3281be3895d45725e3.json"}}, {"family": "Jirou\u0161kov\u00e1", "given": "Mark\u00e9ta", "initials": "M", "orcid": "0009-0004-7173-2177", "researcher": {"href": "https://publications.scilifelab.se/researcher/2f9d163ba6b44bfc8f5e8cf3e6f87e44.json"}}, {"family": "Oliveira", "given": "Daniel V", "initials": "DV", "orcid": "0000-0003-0622-2934", "researcher": {"href": "https://publications.scilifelab.se/researcher/9a7d211d1bfb41c28c667db817d8a7fb.json"}}, {"family": "Frontino", "given": "Anna Maria", "initials": "AM"}, {"family": "Hankeova", "given": "Simona", "initials": "S"}, {"family": "He", "given": "Jingyan", "initials": "J"}, {"family": "Turetti", "given": "Fabio", "initials": "F", "orcid": "0000-0003-0440-5489", "researcher": {"href": "https://publications.scilifelab.se/researcher/3d99dc0f1b3e45439fef4a0c2fed9791.json"}}, {"family": "Iqbal", "given": "Afshan", "initials": "A", "orcid": "0009-0006-4246-9925", "researcher": {"href": "https://publications.scilifelab.se/researcher/d8e111d1327f45caa414225c7a055153.json"}}, {"family": "\u010cervenka", "given": "Igor", "initials": "I"}, {"family": "Sarnov\u00e1", "given": "Lenka", "initials": "L"}, {"family": "Verboven", "given": "Elisabeth", "initials": "E", "orcid": "0000-0003-1432-2791", "researcher": {"href": "https://publications.scilifelab.se/researcher/b760332c736f4516941c50535fd14d71.json"}}, {"family": "Brabec", "given": "Tom\u00e1\u0161", "initials": "T"}, {"family": "Bj\u00f6rkstr\u00f6m", "given": "Niklas K", "initials": "NK"}, {"family": "Gregor", "given": "Martin", "initials": "M", "orcid": "0000-0001-6841-9527", "researcher": {"href": "https://publications.scilifelab.se/researcher/46ae390647a54b0fa1ddfaeb881d1efd.json"}}, {"family": "Dobe\u0161", "given": "Jan", "initials": "J", "orcid": "0000-0003-1853-1603", "researcher": {"href": "https://publications.scilifelab.se/researcher/3587f500b8c041d5b4382df838179d6a.json"}}, {"family": "Andersson", "given": "Emma R", "initials": "ER", "orcid": "0000-0002-8608-625X", "researcher": {"href": "https://publications.scilifelab.se/researcher/1c7313cdcd5f41d4a567a6c315aac3a1.json"}}], "type": "journal article", "published": "2024-11-00", "journal": {"title": "EMBO Mol Med", "issn": "1757-4684", "volume": "16", "issue": "11", "pages": "2946-2975", "issn-l": "1757-4676"}, "abstract": "Fibrosis contributes to tissue repair, but excessive fibrosis disrupts organ function. Alagille syndrome (ALGS, caused by mutations in JAGGED1) results in liver disease and characteristic fibrosis. Here, we show that Jag1Ndr/Ndr mice, a model for ALGS, recapitulate ALGS-like fibrosis. Single-cell RNA-seq and multi-color flow cytometry of the liver revealed immature hepatocytes and paradoxically low intrahepatic T cell infiltration despite cholestasis in Jag1Ndr/Ndr mice. Thymic and splenic regulatory T cells (Tregs) were enriched and Jag1Ndr/Ndr lymphocyte immune and fibrotic capacity was tested with adoptive transfer into Rag1-/- mice, challenged with dextran sulfate sodium (DSS) or bile duct ligation (BDL). Transplanted Jag1Ndr/Ndr lymphocytes were less inflammatory with fewer activated T cells than Jag1+/+ lymphocytes in response to DSS. Cholestasis induced by BDL in Rag1-/- mice with Jag1Ndr/Ndr lymphocytes resulted in periportal Treg accumulation and three-fold less periportal fibrosis than in Rag1-/- mice with Jag1+/+ lymphocytes. Finally, the Jag1Ndr/Ndr hepatocyte expression profile and Treg overrepresentation were corroborated in patients' liver samples. Jag1-dependent hepatic and immune defects thus interact to determine the fibrotic process in ALGS.", "doi": "10.1038/s44321-024-00145-8", "pmid": "39358604", "labels": {"Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC11554675"}, {"db": "pii", "key": "10.1038/s44321-024-00145-8"}], "notes": [], "created": "2024-11-25T10:22:26.021Z", "modified": "2025-02-28T14:16:54.031Z"}]}