{"entity": "researcher", "timestamp": "2026-07-12T09:32:57.843Z", "family": "Hodek", "given": "Ond\u0159ej", "initials": "O", "orcid": "0000-0001-8307-9575", "affiliations": ["Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Linnaeus v\u00e4g 6, 907 36 Ume\u00e5, Sweden. ondrej.hodek@slu.se."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/0e3bcef870804d4f9ec3d3e257cbc323.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/0e3bcef870804d4f9ec3d3e257cbc323"}}, "publications": [{"entity": "publication", "iuid": "0c93730d9dd14ab48b73ff3ca9878f55", "links": {"self": {"href": "https://publications.scilifelab.se/publication/0c93730d9dd14ab48b73ff3ca9878f55.json"}, "display": {"href": "https://publications.scilifelab.se/publication/0c93730d9dd14ab48b73ff3ca9878f55"}}, "title": "Pantothenate kinase 4 controls skeletal muscle substrate metabolism.", "authors": [{"family": "Miranda-Cervantes", "given": "Adriana", "initials": "A", "orcid": "0009-0009-8170-5101", "researcher": {"href": "https://publications.scilifelab.se/researcher/92c3d51b0d5547c2967137ba46c0bc20.json"}}, {"family": "Fritzen", "given": "Andreas M", "initials": "AM", "orcid": "0000-0001-8793-9739", "researcher": {"href": "https://publications.scilifelab.se/researcher/cfe40934c44241ae9c9775e5966e2944.json"}}, {"family": "Raun", "given": "Steffen H", "initials": "SH"}, {"family": "Hodek", "given": "Ond\u0159ej", "initials": "O", "orcid": "0000-0001-8307-9575", "researcher": {"href": "https://publications.scilifelab.se/researcher/0e3bcef870804d4f9ec3d3e257cbc323.json"}}, {"family": "M\u00f8ller", "given": "Lisbeth L V", "initials": "LLV", "orcid": "0000-0003-1885-0102", "researcher": {"href": "https://publications.scilifelab.se/researcher/d5031e341c004fdbb3c48f8159455033.json"}}, {"family": "Johann", "given": "Kornelia", "initials": "K"}, {"family": "Deisen", "given": "Luisa", "initials": "L"}, {"family": "Gregorevic", "given": "Paul", "initials": "P", "orcid": "0000-0002-7418-8945", "researcher": {"href": "https://publications.scilifelab.se/researcher/b0db9536f286420a8cb58c97704ff408.json"}}, {"family": "Gudiksen", "given": "Anders", "initials": "A"}, {"family": "Artati", "given": "Anna", "initials": "A"}, {"family": "Adamski", "given": "Jerzy", "initials": "J", "orcid": "0000-0001-9259-0199", "researcher": {"href": "https://publications.scilifelab.se/researcher/c4906c15dbbf4968aa09d398302578f1.json"}}, {"family": "Andersen", "given": "Nicoline R", "initials": "NR"}, {"family": "Sigvardsen", "given": "Casper M", "initials": "CM", "orcid": "0000-0002-9891-5721", "researcher": {"href": "https://publications.scilifelab.se/researcher/3c01caeb8b5c48fa8b9ef06dd6c2384f.json"}}, {"family": "Carl", "given": "Christian S", "initials": "CS", "orcid": "0000-0002-9551-5837", "researcher": {"href": "https://publications.scilifelab.se/researcher/a831e2cb7b9c465d84407afe5f2f5e02.json"}}, {"family": "Voldstedlund", "given": "Christian T", "initials": "CT", "orcid": "0000-0002-6368-8995", "researcher": {"href": "https://publications.scilifelab.se/researcher/777bfb6f108f4e66a8f04eabbb9c1e1d.json"}}, {"family": "Kj\u00f8bsted", "given": "Rasmus", "initials": "R", "orcid": "0000-0002-0628-4994", "researcher": {"href": "https://publications.scilifelab.se/researcher/dd0c686a41344b249192643a23e9ce69.json"}}, {"family": "Hauck", "given": "Stefanie M", "initials": "SM", "orcid": "0000-0002-1630-6827", "researcher": {"href": "https://publications.scilifelab.se/researcher/dcc26d030c9548598e85094fe036d190.json"}}, {"family": "Schjerling", "given": "Peter", "initials": "P"}, {"family": "Jensen", "given": "Thomas E", "initials": "TE", "orcid": "0000-0001-6139-8268", "researcher": {"href": "https://publications.scilifelab.se/researcher/a36948f538fc4858a69d706e52a3f111.json"}}, {"family": "Cebrian-Serrano", "given": "Alberto", "initials": "A", "orcid": "0000-0002-4737-0215", "researcher": {"href": "https://publications.scilifelab.se/researcher/595cbf9bffd044f1843755c5cf9e4218.json"}}, {"family": "J\u00e4hnert", "given": "Markus", "initials": "M", "orcid": "0000-0001-6459-7318", "researcher": {"href": "https://publications.scilifelab.se/researcher/11a089cfba4d48268b2cf792e01c23ba.json"}}, {"family": "Gottmann", "given": "Pascal", "initials": "P", "orcid": "0000-0002-9791-783X", "researcher": {"href": "https://publications.scilifelab.se/researcher/847d1ef723c74aa58beba753ef3c085c.json"}}, {"family": "Burtscher", "given": "Ingo", "initials": "I"}, {"family": "Lickert", "given": "Heiko", "initials": "H", "orcid": "0000-0002-4597-8825", "researcher": {"href": "https://publications.scilifelab.se/researcher/a96d6282848146d0a627a3b36f79bb42.json"}}, {"family": "Pilegaard", "given": "Henriette", "initials": "H", "orcid": "0000-0002-1071-0327", "researcher": {"href": "https://publications.scilifelab.se/researcher/2592a05febba4a44819b20b5dcac5ce8.json"}}, {"family": "Sch\u00fcrmann", "given": "Annette", "initials": "A", "orcid": "0000-0002-4113-4377", "researcher": {"href": "https://publications.scilifelab.se/researcher/9145876644c8417f829f440cc5a31087.json"}}, {"family": "Tsch\u00f6p", "given": "Matthias H", "initials": "MH", "orcid": "0000-0002-4744-371X", "researcher": {"href": "https://publications.scilifelab.se/researcher/2a16535fb02146598846443de90f925b.json"}}, {"family": "Moritz", "given": "Thomas", "initials": "T", "orcid": "0000-0002-4258-3190", "researcher": {"href": "https://publications.scilifelab.se/researcher/95ad5b7fe48f42eda1328f54a385e097.json"}}, {"family": "M\u00fcller", "given": "Timo D", "initials": "TD", "orcid": "0000-0002-0624-9339", "researcher": {"href": "https://publications.scilifelab.se/researcher/4f16d48a131042c1908b1f9394714fa1.json"}}, {"family": "Sylow", "given": "Lykke", "initials": "L", "orcid": "0000-0003-0905-5932", "researcher": {"href": "https://publications.scilifelab.se/researcher/b8f76299a7e642008b90f4bdee53e6af.json"}}, {"family": "Kiens", "given": "Bente", "initials": "B", "orcid": "0000-0001-5705-5625", "researcher": {"href": "https://publications.scilifelab.se/researcher/4ff3bc60ee9d4402bebf3a96d79a9eed.json"}}, {"family": "Richter", "given": "Erik A", "initials": "EA", "orcid": "0000-0002-6850-3056", "researcher": {"href": "https://publications.scilifelab.se/researcher/6b8efad73a8b45ccab3c9379fc0e5d3e.json"}}, {"family": "Kleinert", "given": "Maximilian", "initials": "M", "orcid": "0000-0002-8069-9055", "researcher": {"href": "https://publications.scilifelab.se/researcher/8ba2dbbe059a44a2b0ad9dcf82a13bf3.json"}}], "type": "journal article", "published": "2025-01-02", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "16", "issue": "1", "pages": "345", "issn-l": "2041-1723"}, "abstract": "Metabolic flexibility in skeletal muscle is essential for maintaining healthy glucose and lipid metabolism, and its dysfunction is closely linked to metabolic diseases. Exercise enhances metabolic flexibility, making it an important tool for discovering mechanisms that promote metabolic health. Here we show that pantothenate kinase 4 (PanK4) is a new conserved exercise target with high abundance in muscle. Muscle-specific deletion of PanK4 impairs fatty acid oxidation which is related to higher intramuscular acetyl-CoA and malonyl-CoA levels. Elevated acetyl-CoA levels persist regardless of feeding state and are associated with whole-body glucose intolerance, reduced insulin-stimulated glucose uptake in glycolytic muscle, and impaired glucose uptake during exercise. Conversely, increasing PanK4 levels in glycolytic muscle lowers acetyl-CoA and enhances glucose uptake. Our findings highlight PanK4 as an important regulator of acetyl-CoA levels, playing a key role in both muscle lipid and glucose metabolism.", "doi": "10.1038/s41467-024-55036-w", "pmid": "39746949", "labels": {"Swedish Metabolomics Centre": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC11695632"}, {"db": "pii", "key": "10.1038/s41467-024-55036-w"}], "notes": [], "created": "2025-11-18T12:03:37.103Z", "modified": "2025-11-18T12:03:38.520Z"}, {"entity": "publication", "iuid": "c7eea563d7774ae5aa7338fb67bc2b74", "links": {"self": {"href": "https://publications.scilifelab.se/publication/c7eea563d7774ae5aa7338fb67bc2b74.json"}, "display": {"href": "https://publications.scilifelab.se/publication/c7eea563d7774ae5aa7338fb67bc2b74"}}, "title": "Reduced adipocyte glutaminase activity promotes energy expenditure and metabolic health.", "authors": [{"family": "Lecoutre", "given": "Simon", "initials": "S", "orcid": "0000-0002-2536-6119", "researcher": {"href": "https://publications.scilifelab.se/researcher/ac1619986a25402b994efa1811b5cfff.json"}}, {"family": "Maqdasy", "given": "Salwan", "initials": "S", "orcid": "0000-0001-5164-9879", "researcher": {"href": "https://publications.scilifelab.se/researcher/809a82e9fa1e4173ba1dd2133a91d5c5.json"}}, {"family": "Rizo-Roca", "given": "David", "initials": "D", "orcid": "0000-0002-9379-7543", "researcher": {"href": "https://publications.scilifelab.se/researcher/52016d47cd9c4dcab663c166cc1afa68.json"}}, {"family": "Renzi", "given": "Gianluca", "initials": "G"}, {"family": "Vlassakev", "given": "Ivan", "initials": "I"}, {"family": "Alaeddine", "given": "Lynn M", "initials": "LM", "orcid": "0000-0001-8494-7514", "researcher": {"href": "https://publications.scilifelab.se/researcher/3d7386fee9ac4d6da8f738bb573302ac.json"}}, {"family": "Higos", "given": "Romane", "initials": "R"}, {"family": "Jalkanen", "given": "Jutta", "initials": "J", "orcid": "0000-0002-0219-1096", "researcher": {"href": "https://publications.scilifelab.se/researcher/96620636aff142c484d07894c573f3d2.json"}}, {"family": "Zhong", "given": "Jiawei", "initials": "J", "orcid": "0000-0002-2802-0770", "researcher": {"href": "https://publications.scilifelab.se/researcher/3d30cbe9680a42c1b6478921e4b63287.json"}}, {"family": "Zareifi", "given": "Danae S", "initials": "DS", "orcid": "0000-0003-2108-4955", "researcher": {"href": "https://publications.scilifelab.se/researcher/90ffdf76251f455bb8f15d9de4f0e86a.json"}}, {"family": "Frendo-Cumbo", "given": "Scott", "initials": "S", "orcid": "0000-0002-9047-4650", "researcher": {"href": "https://publications.scilifelab.se/researcher/4bfa1b27416e47fd90a77657fcb0a34e.json"}}, {"family": "Massier", "given": "Lucas", "initials": "L", "orcid": "0000-0002-6199-2643", "researcher": {"href": "https://publications.scilifelab.se/researcher/94411e29fc224eb3b8b215f984f05ee9.json"}}, {"family": "Hodek", "given": "Ondrej", "initials": "O", "orcid": "0000-0001-8307-9575", "researcher": {"href": "https://publications.scilifelab.se/researcher/0e3bcef870804d4f9ec3d3e257cbc323.json"}}, {"family": "Juvany", "given": "Marta", "initials": "M"}, {"family": "Moritz", "given": "Thomas", "initials": "T"}, {"family": "de Castro Barbosa", "given": "Thais", "initials": "T", "orcid": "0000-0002-3040-9873", "researcher": {"href": "https://publications.scilifelab.se/researcher/b8991ec28185464eaf5c8ee076b9d071.json"}}, {"family": "Omar-Hmeadi", "given": "Muhmmad", "initials": "M", "orcid": "0000-0001-8893-7348", "researcher": {"href": "https://publications.scilifelab.se/researcher/4776032637a2418682320dc68bf9417f.json"}}, {"family": "L\u00f3pez-Yus", "given": "Marta", "initials": "M", "orcid": "0000-0002-5822-9823", "researcher": {"href": "https://publications.scilifelab.se/researcher/ecc979a3cc074d69860f44f6c51a1d1b.json"}}, {"family": "Merabtene", "given": "Fatiha", "initials": "F"}, {"family": "Abatan", "given": "Jimon Boniface", "initials": "JB"}, {"family": "Marcelin", "given": "Genevi\u00e8ve", "initials": "G"}, {"family": "El Hachem", "given": "Elie-Julien", "initials": "EJ", "orcid": "0000-0001-5033-6117", "researcher": {"href": "https://publications.scilifelab.se/researcher/0862046052b9458fa7710d2263a133e2.json"}}, {"family": "Rouault", "given": "Christine", "initials": "C"}, {"family": "Bergo", "given": "Martin O", "initials": "MO"}, {"family": "Petrus", "given": "Paul", "initials": "P", "orcid": "0000-0001-8297-3874", "researcher": {"href": "https://publications.scilifelab.se/researcher/baffd8ee268542d6a3702ab97b985d14.json"}}, {"family": "Zierath", "given": "Juleen R", "initials": "JR", "orcid": "0000-0001-6891-7497", "researcher": {"href": "https://publications.scilifelab.se/researcher/f4ef2b94ba2d44dd9b41192567a7af53.json"}}, {"family": "Cl\u00e9ment", "given": "Karine", "initials": "K", "orcid": "0000-0002-2489-3355", "researcher": {"href": "https://publications.scilifelab.se/researcher/840fc2b0de56464cabf3c130dfedf57f.json"}}, {"family": "Krook", "given": "Anna", "initials": "A", "orcid": "0000-0002-0891-0258", "researcher": {"href": "https://publications.scilifelab.se/researcher/f43339bcd571486b845a3b10e241bcc4.json"}}, {"family": "Mejhert", "given": "Niklas", "initials": "N", "orcid": "0000-0003-1785-833X", "researcher": {"href": "https://publications.scilifelab.se/researcher/81eb60b84ea14ad9ba7e4d3433c37a3e.json"}}, {"family": "Ryd\u00e9n", "given": "Mikael", "initials": "M", "orcid": "0000-0003-4785-1876", "researcher": {"href": "https://publications.scilifelab.se/researcher/ab61ac66c36749019ce5066fb1a5061e.json"}}], "type": "journal article", "published": "2024-07-00", "journal": {"title": "Nat Metab", "issn": "2522-5812", "volume": "6", "issue": "7", "pages": "1329-1346", "issn-l": "2522-5812"}, "abstract": "Glutamine and glutamate are interconverted by several enzymes and alterations in this metabolic cycle are linked to cardiometabolic traits. Herein, we show that obesity-associated insulin resistance is characterized by decreased plasma and white adipose tissue glutamine-to-glutamate ratios. We couple these stoichiometric changes to perturbed fat cell glutaminase and glutamine synthase messenger RNA and protein abundance, which together promote glutaminolysis. In human white adipocytes, reductions in glutaminase activity promote aerobic glycolysis and mitochondrial oxidative capacity via increases in hypoxia-inducible factor 1\u03b1 abundance, lactate levels and p38 mitogen-activated protein kinase signalling. Systemic glutaminase inhibition in male and female mice, or genetically in adipocytes of male mice, triggers the activation of thermogenic gene programs in inguinal adipocytes. Consequently, the knockout mice display higher energy expenditure and improved glucose tolerance compared to control littermates, even under high-fat diet conditions. Altogether, our findings highlight white adipocyte glutamine turnover as an important determinant of energy expenditure and metabolic health.", "doi": "10.1038/s42255-024-01083-y", "pmid": "39009762", "labels": {"Swedish Metabolomics Centre": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC11272588"}, {"db": "pii", "key": "10.1038/s42255-024-01083-y"}], "notes": [], "created": "2024-11-26T10:26:41.024Z", "modified": "2025-10-17T13:03:13.077Z"}, {"entity": "publication", "iuid": "12f24de0714c4426bd445a0e28fb18c0", "links": {"self": {"href": "https://publications.scilifelab.se/publication/12f24de0714c4426bd445a0e28fb18c0.json"}, "display": {"href": "https://publications.scilifelab.se/publication/12f24de0714c4426bd445a0e28fb18c0"}}, "title": "Formate overflow drives toxic folate trapping in MTHFD1 inhibited cancer cells.", "authors": [{"family": "Green", "given": "Alanna C", "initials": "AC", "orcid": "0000-0002-0175-1485", "researcher": {"href": "https://publications.scilifelab.se/researcher/b3c3193266a24fafb796e21b0a95c6cb.json"}}, {"family": "Marttila", "given": "Petra", "initials": "P", "orcid": "0000-0002-0115-8067", "researcher": {"href": "https://publications.scilifelab.se/researcher/2e3e92bdf843456784f98d3b521d2ccd.json"}}, {"family": "Kiweler", "given": "Nicole", "initials": "N", "orcid": "0000-0001-7493-703X", "researcher": {"href": "https://publications.scilifelab.se/researcher/f8b0eaee2d574b4daba1ee735c512797.json"}}, {"family": "Chalkiadaki", "given": "Christina", "initials": "C"}, {"family": "Wiita", "given": "Elis\u00e9e", "initials": "E"}, {"family": "Cookson", "given": "Victoria", "initials": "V"}, {"family": "Lesur", "given": "Antoine", "initials": "A"}, {"family": "Eiden", "given": "Kim", "initials": "K"}, {"family": "Bernardin", "given": "Fran\u00e7ois", "initials": "F", "orcid": "0000-0002-6329-5542", "researcher": {"href": "https://publications.scilifelab.se/researcher/fc67be6d569140d3afa6d923a7b85e9d.json"}}, {"family": "Vallin", "given": "Karl S A", "initials": "KSA"}, {"family": "Borhade", "given": "Sanjay", "initials": "S"}, {"family": "Long", "given": "Maeve", "initials": "M"}, {"family": "Ghahe", "given": "Elahe Kamali", "initials": "EK"}, {"family": "Jim\u00e9nez-Alonso", "given": "Julio J", "initials": "JJ"}, {"family": "Jemth", "given": "Ann-Sofie", "initials": "AS", "orcid": "0000-0002-7550-1833", "researcher": {"href": "https://publications.scilifelab.se/researcher/fd07c6c543544af1a904e039f73ba857.json"}}, {"family": "Loseva", "given": "Olga", "initials": "O"}, {"family": "Mortusewicz", "given": "Oliver", "initials": "O", "orcid": "0000-0002-4290-4994", "researcher": {"href": "https://publications.scilifelab.se/researcher/b62a88fc39a54906a40e28e679c9f624.json"}}, {"family": "Meyers", "given": "Marianne", "initials": "M"}, {"family": "Viry", "given": "Elodie", "initials": "E"}, {"family": "Johansson", "given": "Annika I", "initials": "AI", "orcid": "0000-0001-5000-1288", "researcher": {"href": "https://publications.scilifelab.se/researcher/0b0835b94db946929c1cb0c8f9319068.json"}}, {"family": "Hodek", "given": "Ond\u0159ej", "initials": "O", "orcid": "0000-0001-8307-9575", "researcher": {"href": "https://publications.scilifelab.se/researcher/0e3bcef870804d4f9ec3d3e257cbc323.json"}}, {"family": "Homan", "given": "Evert", "initials": "E"}, {"family": "Bonagas", "given": "Nadilly", "initials": "N", "orcid": "0000-0001-7336-4065", "researcher": {"href": "https://publications.scilifelab.se/researcher/1e38f3b0752740969c5c13fb77885b27.json"}}, {"family": "Ramos", "given": "Louise", "initials": "L", "orcid": "0000-0002-3808-6853", "researcher": {"href": "https://publications.scilifelab.se/researcher/fe708067508a48eba89caed34f06be36.json"}}, {"family": "Sandberg", "given": "Lars", "initials": "L"}, {"family": "Fr\u00f6din", "given": "Morten", "initials": "M"}, {"family": "Moussay", "given": "Etienne", "initials": "E", "orcid": "0000-0002-0879-8067", "researcher": {"href": "https://publications.scilifelab.se/researcher/28965f7f1da94e969c5e16ec594462e2.json"}}, {"family": "Slipicevic", "given": "Ana", "initials": "A"}, {"family": "Letellier", "given": "Elisabeth", "initials": "E", "orcid": "0000-0001-8242-9393", "researcher": {"href": "https://publications.scilifelab.se/researcher/dc06ccab76e24f2d88755bb90b713423.json"}}, {"family": "Paggetti", "given": "J\u00e9r\u00f4me", "initials": "J", "orcid": "0000-0001-9460-5876", "researcher": {"href": "https://publications.scilifelab.se/researcher/19962ab6e6974b35946c660a2c7551e5.json"}}, {"family": "S\u00f8rensen", "given": "Claus Storgaard", "initials": "CS"}, {"family": "Helleday", "given": "Thomas", "initials": "T", "orcid": "0000-0002-7384-092X", "researcher": {"href": "https://publications.scilifelab.se/researcher/3d7256c271ea4adea404d4ff355f804e.json"}}, {"family": "Henriksson", "given": "Martin", "initials": "M", "orcid": "0000-0002-2230-4887", "researcher": {"href": "https://publications.scilifelab.se/researcher/8ad0e6d72bac4962805ba267db810a90.json"}}, {"family": "Meiser", "given": "Johannes", "initials": "J", "orcid": "0000-0002-9093-6210", "researcher": {"href": "https://publications.scilifelab.se/researcher/b83edb0c60e54fc9a26ddfb3165d069b.json"}}], "type": "journal article", "published": "2023-04-00", "journal": {"title": "Nat Metab", "issn": "2522-5812", "issn-l": "2522-5812", "volume": "5", "issue": "4", "pages": "642-659"}, "abstract": "Cancer cells fuel their increased need for nucleotide supply by upregulating one-carbon (1C) metabolism, including the enzymes methylenetetrahydrofolate dehydrogenase-cyclohydrolase 1 and 2 (MTHFD1 and MTHFD2). TH9619 is a potent inhibitor of dehydrogenase and cyclohydrolase activities in both MTHFD1 and MTHFD2, and selectively kills cancer cells. Here, we reveal that, in cells, TH9619 targets nuclear MTHFD2 but does not inhibit mitochondrial MTHFD2. Hence, overflow of formate from mitochondria continues in the presence of TH9619. TH9619 inhibits the activity of MTHFD1 occurring downstream of mitochondrial formate release, leading to the accumulation of 10-formyl-tetrahydrofolate, which we term a 'folate trap'. This results in thymidylate depletion and death of MTHFD2-expressing cancer cells. This previously uncharacterized folate trapping mechanism is exacerbated by physiological hypoxanthine levels that block the de novo purine synthesis pathway, and additionally prevent 10-formyl-tetrahydrofolate consumption for purine synthesis. The folate trapping mechanism described here for TH9619 differs from other MTHFD1/2 inhibitors and antifolates. Thus, our findings uncover an approach to attack cancer and reveal a regulatory mechanism in 1C metabolism.", "doi": "10.1038/s42255-023-00771-5", "pmid": "37012496", "labels": {"Swedish Metabolomics Centre": "Collaborative", "Drug Discovery and Development": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC10132981"}, {"db": "pii", "key": "10.1038/s42255-023-00771-5"}], "notes": [], "created": "2023-08-30T07:09:03.400Z", "modified": "2025-10-17T13:05:07.625Z"}, {"entity": "publication", "iuid": "2560c871bef6447c997e5e85e55cb5df", "links": {"self": {"href": "https://publications.scilifelab.se/publication/2560c871bef6447c997e5e85e55cb5df.json"}, "display": {"href": "https://publications.scilifelab.se/publication/2560c871bef6447c997e5e85e55cb5df"}}, "title": "Mixed-mode chromatography-mass spectrometry enables targeted and untargeted screening of carboxylic acids in biological samples.", "authors": [{"family": "Hodek", "given": "Ond\u0159ej", "initials": "O", "orcid": "0000-0001-8307-9575", "researcher": {"href": "https://publications.scilifelab.se/researcher/0e3bcef870804d4f9ec3d3e257cbc323.json"}}, {"family": "Argemi-Muntadas", "given": "Lidia", "initials": "L"}, {"family": "Khan", "given": "Adnan", "initials": "A"}, {"family": "Moritz", "given": "Thomas", "initials": "T"}], "type": "journal article", "published": "2022-03-10", "journal": {"title": "Anal. Methods", "issn": "1759-9679", "volume": "14", "issue": "10", "pages": "1015-1022", "issn-l": "1759-9660"}, "abstract": "Carboxylic acids are crucial metabolites in the tricarboxylic acid (TCA) cycle and thus participate in central carbon metabolism (CCM). Research dependent on the analysis of metabolites involved in central carbon metabolism requires fast separation and sensitive detection of carboxylic acids using liquid chromatography-mass spectrometry (LC-MS). However, successful separation of all carboxylic acids from the TCA cycle by liquid chromatography remains a challenging task because of their high polarity and thus low retention on the conventional reversed-phase columns. In this study, we tested a reversed-phase/anion exchange mixed-mode stationary phase (Waters BEH C18 AX) using liquid chromatography-tandem mass spectrometry (LC-MS/MS). We developed and optimized a method that enables a 10 minute separation of all carboxylic acids from the TCA cycle and lactic acid without prior derivatization or addition of ion-pair reagents in the mobile phase. The developed method was validated for quantification of 8 acids in murine brown preadipocytes, 5 acids in human plasma and 6 acids in Arabidopsis thaliana leaves with limits of quantification ranging from 0.1 \u03bcM for malic acid to 10 \u03bcM for isocitric acid. Moreover, the mixed-mode chromatography enabled untargeted screening of medium- to long-chain fatty acids in murine brown preadipocytes, Arabidopsis thaliana, and human plasma, where 23 fatty acids were identified by using liquid chromatography with high-resolution mass spectrometry (HRMS).", "doi": "10.1039/d1ay02143e", "pmid": "35195623", "labels": {"Swedish Metabolomics Centre": "Technology development"}, "xrefs": [], "notes": [], "created": "2022-12-05T08:17:51.559Z", "modified": "2025-10-17T13:03:15.275Z"}]}