{"entity": "researcher", "timestamp": "2026-03-17T02:56:08.981Z", "family": "Chanez", "given": "C", "initials": "C", "orcid": "0000-0002-3432-4375", "affiliations": ["Department of Biochemistry, University of Z\u00fcrich, 8057 Z\u00fcrich, Switzerland."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/0ce66636fd1344819df3518ffa6406ce.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/0ce66636fd1344819df3518ffa6406ce"}}, "publications": [{"entity": "publication", "iuid": "47741b433ff341d898259372903d7f89", "links": {"self": {"href": "https://publications.scilifelab.se/publication/47741b433ff341d898259372903d7f89.json"}, "display": {"href": "https://publications.scilifelab.se/publication/47741b433ff341d898259372903d7f89"}}, "title": "Massively parallel analysis of single-molecule dynamics on next-generation sequencing chips.", "authors": [{"family": "Aguirre Rivera", "given": "J", "initials": "J", "orcid": "0000-0002-5263-3577", "researcher": {"href": "https://publications.scilifelab.se/researcher/f164778943f24897a1fa31ac3c6fd2ce.json"}}, {"family": "Mao", "given": "G", "initials": "G", "orcid": "0000-0001-5841-0991", "researcher": {"href": "https://publications.scilifelab.se/researcher/827d755ddbd24c2ca839364c9e139965.json"}}, {"family": "Sabantsev", "given": "A", "initials": "A", "orcid": "0000-0002-8559-8894", "researcher": {"href": "https://publications.scilifelab.se/researcher/ae73150df15e4730be688fc5137a1389.json"}}, {"family": "Panfilov", "given": "M", "initials": "M", "orcid": "0000-0003-2875-7315", "researcher": {"href": "https://publications.scilifelab.se/researcher/58d227ea91aa4b90915a7e2afeb9283e.json"}}, {"family": "Hou", "given": "Q", "initials": "Q", "orcid": "0009-0007-9296-4788", "researcher": {"href": "https://publications.scilifelab.se/researcher/e1048338ae024e9fbc9d82d79f61ffee.json"}}, {"family": "Lindell", "given": "M", "initials": "M"}, {"family": "Chanez", "given": "C", "initials": "C", "orcid": "0000-0002-3432-4375", "researcher": {"href": "https://publications.scilifelab.se/researcher/0ce66636fd1344819df3518ffa6406ce.json"}}, {"family": "Ritort", "given": "F", "initials": "F", "orcid": "0000-0002-4869-5003", "researcher": {"href": "https://publications.scilifelab.se/researcher/f7a34940c8ae42f1a2381b0b48fe06be.json"}}, {"family": "Jinek", "given": "M", "initials": "M", "orcid": "0000-0002-7601-210X", "researcher": {"href": "https://publications.scilifelab.se/researcher/5b86b5f94bbf4b87849dd4d8cebf80f8.json"}}, {"family": "Deindl", "given": "S", "initials": "S", "orcid": "0000-0001-6807-8654", "researcher": {"href": "https://publications.scilifelab.se/researcher/2e45e8288a3445e2b346f29b73141738.json"}}], "type": "journal article", "published": "2024-08-23", "journal": {"title": "Science", "issn": "1095-9203", "issn-l": "0036-8075", "volume": "385", "issue": "6711", "pages": "892-898"}, "abstract": "Single-molecule techniques are ideally poised to characterize complex dynamics but are typically limited to investigating a small number of different samples. However, a large sequence or chemical space often needs to be explored to derive a comprehensive understanding of complex biological processes. Here we describe multiplexed single-molecule characterization at the library scale (MUSCLE), a method that combines single-molecule fluorescence microscopy with next-generation sequencing to enable highly multiplexed observations of complex dynamics. We comprehensively profiled the sequence dependence of DNA hairpin properties and Cas9-induced target DNA unwinding-rewinding dynamics. The ability to explore a large sequence space for Cas9 allowed us to identify a number of target sequences with unexpected behaviors. We envision that MUSCLE will enable the mechanistic exploration of many fundamental biological processes.", "doi": "10.1126/science.adn5371", "pmid": "39172826", "labels": {"NGI Short read": "Technology development", "NGI Uppsala (SNP&SEQ Technology Platform)": "Technology development", "National Genomics Infrastructure": "Technology development"}, "xrefs": [], "notes": [], "created": "2024-08-23T12:11:46.194Z", "modified": "2024-11-05T18:25:16.868Z"}]}