{"entity": "researcher", "timestamp": "2026-07-20T01:06:26.497Z", "family": "Bacic", "given": "Luka", "initials": "L", "orcid": "0000-0001-6896-3506", "affiliations": ["Department of Cell and Molecular Biology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/0bcd56cafd294116870c894ba344cf12.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/0bcd56cafd294116870c894ba344cf12"}}, "publications": [{"entity": "publication", "iuid": "c7b4c0e889ea4415b8cbaa01eccb847b", "links": {"self": {"href": "https://publications.scilifelab.se/publication/c7b4c0e889ea4415b8cbaa01eccb847b.json"}, "display": {"href": "https://publications.scilifelab.se/publication/c7b4c0e889ea4415b8cbaa01eccb847b"}}, "title": "ALC1 Finds a New Foothold on the Nucleosome\u2019s Super-Groove", "authors": [{"family": "Bridges", "given": "Hannah R", "initials": "HR", "orcid": "0000-0001-6890-6050", "researcher": {"href": "https://publications.scilifelab.se/researcher/87b42fbd72ad4a25949e7bb7113dd0b1.json"}}, {"family": "Bacic", "given": "Luka", "initials": "L", "orcid": "0000-0001-6896-3506", "researcher": {"href": "https://publications.scilifelab.se/researcher/0bcd56cafd294116870c894ba344cf12.json"}}, {"family": "Deindl", "given": "Sebastian", "initials": "S", "orcid": "0000-0001-6807-8654", "researcher": {"href": "https://publications.scilifelab.se/researcher/2e45e8288a3445e2b346f29b73141738.json"}}, {"family": "Gaullier", "given": "Guillaume", "initials": "G", "orcid": "0000-0003-3405-6021", "researcher": {"href": "https://publications.scilifelab.se/researcher/26bde2020c164cff8216338fb2d6d652.json"}}], "type": "posted-content", "published": "2025-11-10", "journal": {"title": "biorxiv", "issn": null, "issn-l": null, "volume": null, "issue": null, "pages": null}, "abstract": null, "doi": "10.1101/2025.11.10.687450", "pmid": null, "labels": {"Cryo-EM": "Service"}, "xrefs": [], "notes": [], "created": "2025-11-17T10:09:15.163Z", "modified": "2025-12-18T18:59:43.530Z"}, {"entity": "publication", "iuid": "0558978a6cc14afe8e55e57e9b14b996", "links": {"self": {"href": "https://publications.scilifelab.se/publication/0558978a6cc14afe8e55e57e9b14b996.json"}, "display": {"href": "https://publications.scilifelab.se/publication/0558978a6cc14afe8e55e57e9b14b996"}}, "title": "Structure and dynamics of the chromatin remodeler ALC1 bound to a PARylated nucleosome.", "authors": [{"family": "Bacic", "given": "Luka", "initials": "L", "orcid": "0000-0001-6896-3506", "researcher": {"href": "https://publications.scilifelab.se/researcher/0bcd56cafd294116870c894ba344cf12.json"}}, {"family": "Gaullier", "given": "Guillaume", "initials": "G", "orcid": "0000-0003-3405-6021", "researcher": {"href": "https://publications.scilifelab.se/researcher/26bde2020c164cff8216338fb2d6d652.json"}}, {"family": "Sabantsev", "given": "Anton", "initials": "A", "orcid": "0000-0002-8559-8894", "researcher": {"href": "https://publications.scilifelab.se/researcher/ae73150df15e4730be688fc5137a1389.json"}}, {"family": "Lehmann", "given": "Laura C", "initials": "LC", "orcid": "0000-0003-2518-5606", "researcher": {"href": "https://publications.scilifelab.se/researcher/e73ae6d30ebe4c68971f3b0908d061c6.json"}}, {"family": "Brackmann", "given": "Klaus", "initials": "K"}, {"family": "Dimakou", "given": "Despoina", "initials": "D", "orcid": "0000-0002-1424-5469", "researcher": {"href": "https://publications.scilifelab.se/researcher/aba59232af334167a0574861f7e921ca.json"}}, {"family": "Halic", "given": "Mario", "initials": "M", "orcid": "0000-0002-0061-7372", "researcher": {"href": "https://publications.scilifelab.se/researcher/12af3cfa4f55436ea4fc7d64c378229e.json"}}, {"family": "Hewitt", "given": "Graeme", "initials": "G"}, {"family": "Boulton", "given": "Simon J", "initials": "SJ"}, {"family": "Deindl", "given": "Sebastian", "initials": "S", "orcid": "0000-0001-6807-8654", "researcher": {"href": "https://publications.scilifelab.se/researcher/2e45e8288a3445e2b346f29b73141738.json"}}], "type": "journal article", "published": "2021-09-06", "journal": {"title": "Elife", "issn": "2050-084X", "volume": "10", "issn-l": "2050-084X"}, "abstract": "The chromatin remodeler ALC1 is recruited to and activated by DNA damage-induced poly(ADP-ribose) (PAR) chains deposited by PARP1/PARP2/HPF1 upon detection of DNA lesions. ALC1 has emerged as a candidate drug target for cancer therapy as its loss confers synthetic lethality in homologous recombination-deficient cells. However, structure-based drug design and molecular analysis of ALC1 have been hindered by the requirement for PARylation and the highly heterogeneous nature of this post-translational modification. Here, we reconstituted an ALC1 and PARylated nucleosome complex modified in vitro using PARP2 and HPF1. This complex was amenable to cryo-EM structure determination without cross-linking, which enabled visualization of several intermediate states of ALC1 from the recognition of the PARylated nucleosome to the tight binding and activation of the remodeler. Functional biochemical assays with PARylated nucleosomes highlight the importance of nucleosomal epitopes for productive remodeling and suggest that ALC1 preferentially slides nucleosomes away from DNA breaks.", "doi": "10.7554/eLife.71420", "pmid": "34486521", "labels": {"Cryo-EM": "Service"}, "xrefs": [{"db": "pii", "key": "71420"}, {"db": "pmc", "key": "PMC8463071"}], "notes": [], "created": "2021-11-03T13:44:23.365Z", "modified": "2021-12-10T13:27:15.631Z"}, {"entity": "publication", "iuid": "6878fad3e80b4b2d84ef0cf49160ba6b", "links": {"self": {"href": "https://publications.scilifelab.se/publication/6878fad3e80b4b2d84ef0cf49160ba6b.json"}, "display": {"href": "https://publications.scilifelab.se/publication/6878fad3e80b4b2d84ef0cf49160ba6b"}}, "title": "Mechanistic Insights into Regulation of the ALC1 Remodeler by the Nucleosome Acidic Patch.", "authors": [{"family": "Lehmann", "given": "Laura C", "initials": "LC", "orcid": "0000-0003-2518-5606", "researcher": {"href": "https://publications.scilifelab.se/researcher/e73ae6d30ebe4c68971f3b0908d061c6.json"}}, {"family": "Bacic", "given": "Luka", "initials": "L", "orcid": "0000-0001-6896-3506", "researcher": {"href": "https://publications.scilifelab.se/researcher/0bcd56cafd294116870c894ba344cf12.json"}}, {"family": "Hewitt", "given": "Graeme", "initials": "G"}, {"family": "Brackmann", "given": "Klaus", "initials": "K"}, {"family": "Sabantsev", "given": "Anton", "initials": "A", "orcid": "0000-0002-8559-8894", "researcher": {"href": "https://publications.scilifelab.se/researcher/ae73150df15e4730be688fc5137a1389.json"}}, {"family": "Gaullier", "given": "Guillaume", "initials": "G", "orcid": "0000-0003-3405-6021", "researcher": {"href": "https://publications.scilifelab.se/researcher/26bde2020c164cff8216338fb2d6d652.json"}}, {"family": "Pytharopoulou", "given": "Sofia", "initials": "S"}, {"family": "Degliesposti", "given": "Gianluca", "initials": "G"}, {"family": "Okkenhaug", "given": "Hanneke", "initials": "H"}, {"family": "Tan", "given": "Song", "initials": "S"}, {"family": "Costa", "given": "Alessandro", "initials": "A"}, {"family": "Skehel", "given": "J Mark", "initials": "JM"}, {"family": "Boulton", "given": "Simon J", "initials": "SJ", "orcid": "0000-0001-6936-6834", "researcher": {"href": "https://publications.scilifelab.se/researcher/8be210bb12b54a19b4020803fa0c3357.json"}}, {"family": "Deindl", "given": "Sebastian", "initials": "S", "orcid": "0000-0001-6807-8654", "researcher": {"href": "https://publications.scilifelab.se/researcher/2e45e8288a3445e2b346f29b73141738.json"}}], "type": "journal article", "published": "2020-12-22", "journal": {"title": "Cell Rep", "issn": "2211-1247", "issn-l": null, "volume": "33", "issue": "12", "pages": "108529"}, "abstract": "Upon DNA damage, the ALC1/CHD1L nucleosome remodeling enzyme (remodeler) is activated by binding to poly(ADP-ribose). How activated ALC1 recognizes the nucleosome, as well as how this recognition is coupled to remodeling, is unknown. Here, we show that remodeling by ALC1 requires a wild-type acidic patch on the entry side of the nucleosome. The cryo-electron microscopy structure of a nucleosome-ALC1 linker complex reveals a regulatory linker segment that binds to the acidic patch. Mutations within this interface alter the dynamics of ALC1 recruitment to DNA damage and impede the ATPase and remodeling activities of ALC1. Full activation requires acidic patch-linker segment interactions that tether the remodeler to the nucleosome and couple ATP hydrolysis to nucleosome mobilization. Upon DNA damage, such a requirement may be used to modulate ALC1 activity via changes in the nucleosome acidic patches.", "doi": "10.1016/j.celrep.2020.108529", "pmid": "33357431", "labels": {"Cryo-EM": "Service"}, "xrefs": [{"db": "pii", "key": "S2211-1247(20)31518-7"}, {"db": "pmc", "key": "PMC7116876"}, {"db": "mid", "key": "EMS117809"}], "notes": [], "created": "2021-03-16T07:24:16.174Z", "modified": "2023-12-04T10:15:55.187Z"}]}